AT1G64185

glyoxalase I family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003849
Physical Location & Seq
Forward (+)
23824487 .. 23825383
897 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64185.1

Sequence Viewer

Length: 357 bp
ATGGCAACGGCGTCGTTTAGGTGGATCTTGCAGCTCCACAGAGACGTACCAAAAGCGGCTCGATTCTATGAGAAAGGTCTCGATTTCTCCGTCAATGTCGTTACCTTACGTTGGGCTGAACTTCAGTCAGGTCCCCTCAAGCTAGCTCTTATGCAAGCTCCCAGCGAGCATGTGATGAGTGAGAAAGGATACTCTTCGTTACTATCGTTCACTGTAGCAGACATTAATACAACAATCTCTAAACTTATGGAATTAGGAGCTGAACTCGATGGCTCTATCAAATATGAAGTCCATGGCAAGGTTGCTTCTGTGAGATGTCTTGATGGTCATGTGCTCGGACTCTATGAACCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

118

Amino Acids

13.07

Weight (kDa)

6.4

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 9 - 104 4.4e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 56
AclWI GGATC 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 2 cut(s) 111, 298
AluBI AGCT 5 cut(s) 34, 142, 146, 158, 260
AluI AGCT 5 cut(s) 34, 142, 146, 158, 260
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 2 cut(s) 36, 83
AlwI GGATC 1 cut(s) 32
ApeKI GCWGC 1 cut(s) 31
ArsI GACNNNNNNTTYG 2 cut(s) 273, 305
AseI ATTAAT 1 cut(s) 225
AspS9I GGNCC 1 cut(s) 131
AsuNHI GCTAGC 1 cut(s) 142
AvaII GGWCC 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 336
BbvI GCAGC 1 cut(s) 43
BccI CCATC 2 cut(s) 263, 317
BceAI ACGGC 1 cut(s) 24
BcgI CGANNNNNNTGC 1 cut(s) 28
BciVI GTATCC 1 cut(s) 182
BcoDI GTCTC 2 cut(s) 36, 83
BfaI CTAG 1 cut(s) 143
BfmI CTRYAG 1 cut(s) 213
BfuI GTATCC 1 cut(s) 182
BisI GCNGC 2 cut(s) 32, 57
BlsI GCNGC 2 cut(s) 33, 58
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 133
BmtI GCTAGC 1 cut(s) 146
BplI GAGNNNNNCTC 2 cut(s) 249, 281
BpuEI CTTGAG 1 cut(s) 122
BsaHI GRCGYC 1 cut(s) 11
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 1 cut(s) 292
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 298
BseDI CCNNGG 1 cut(s) 292
BseLI CCNNNNNNNGG 2 cut(s) 111, 298
BseXI GCAGC 1 cut(s) 43
BseYI CCCAGC 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 336
BslFI GGGAC 1 cut(s) 117
BslI CCNNNNNNNGG 2 cut(s) 111, 298
BsmAI GTCTC 2 cut(s) 36, 83
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 1 cut(s) 117
Bso31I GGTCTC 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 1 cut(s) 24
Bsp19I CCATGG 1 cut(s) 292
BspACI CCGC 1 cut(s) 56
BspHI TCATGA 1 cut(s) 353
BspLI GGNNCC 1 cut(s) 133
BspOI GCTAGC 1 cut(s) 146
BspPI GGATC 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 292
BssMI GATC 1 cut(s) 24
BssNI GRCGYC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 292
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 1 cut(s) 199
BstACI GRCGYC 1 cut(s) 11
BstC8I GCNNGC 3 cut(s) 144, 156, 167
BstDSI CCRYGG 1 cut(s) 292
BstKTI GATC 1 cut(s) 27
BstMAI GTCTC 2 cut(s) 36, 83
BstMBI GATC 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 173
BstSFI CTRYAG 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 43
BstX2I RGATCY 1 cut(s) 24
BstYI RGATCY 1 cut(s) 24
BsuI GTATCC 1 cut(s) 182
BtgI CCRYGG 1 cut(s) 292
BtsIMutI CAGTG 1 cut(s) 210
Cac8I GCNNGC 3 cut(s) 144, 156, 167
CciI TCATGA 1 cut(s) 353
Cfr13I GGNCC 1 cut(s) 131
Csp6I GTAC 1 cut(s) 47
CviAII CATG 4 cut(s) 170, 293, 329, 354
CviJI RGCY 8 cut(s) 34, 59, 116, 142, 146, 158, 260, 273
CviKI_1 RGCY 8 cut(s) 34, 59, 116, 142, 146, 158, 260, 273
CviQI GTAC 1 cut(s) 47
DpnI GATC 1 cut(s) 26
DpnII GATC 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 292
Eco31I GGTCTC 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 292
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 4 cut(s) 173, 296, 332, 357
FaiI YATR 9 cut(s) 69, 152, 171, 248, 285, 294, 330, 345, 355
FaqI GGGAC 1 cut(s) 117
FatI CATG 4 cut(s) 169, 292, 328, 353
Fnu4HI GCNGC 2 cut(s) 32, 57
Fsp4HI GCNGC 2 cut(s) 32, 57
FspBI CTAG 1 cut(s) 143
GluI GCNGC 2 cut(s) 32, 57
GsaI CCCAGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 4 cut(s) 173, 296, 332, 357
HinfI GANTC 2 cut(s) 63, 339
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 1 cut(s) 338
Hpy188III TCNNGA 3 cut(s) 80, 320, 354
Hpy8I GTNNAC 1 cut(s) 210
Hpy99I CGWCG 1 cut(s) 16
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 2 cut(s) 45, 109
HpyCH4V TGCA 2 cut(s) 31, 154
HpySE526I ACGT 2 cut(s) 45, 109
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 4 cut(s) 173, 296, 332, 357
Kzo9I GATC 1 cut(s) 24
LmnI GCTCC 3 cut(s) 39, 163, 257
LpnPI CCDG 2 cut(s) 114, 175
Lsp1109I GCAGC 1 cut(s) 43
MaeI CTAG 1 cut(s) 143
MaeII ACGT 2 cut(s) 45, 109
MaeIII GTNAC 2 cut(s) 100, 198
MalI GATC 1 cut(s) 26
MboI GATC 1 cut(s) 24
MboII GAAGA 1 cut(s) 186
MflI RGATCY 1 cut(s) 24
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 1 cut(s) 251
MlyI GAGTC 1 cut(s) 333
MnlI CCTC 1 cut(s) 146
MseI TTAA 1 cut(s) 225
NcoI CCATGG 1 cut(s) 292
NdeII GATC 1 cut(s) 24
NheI GCTAGC 1 cut(s) 142
NlaIII CATG 4 cut(s) 173, 296, 332, 357
NlaIV GGNNCC 1 cut(s) 133
NspI RCATGY 1 cut(s) 173
PagI TCATGA 1 cut(s) 353
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 203
PfeI GAWTC 1 cut(s) 63
PkrI GCNGC 2 cut(s) 33, 58
PleI GAGTC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 333
PpuMI RGGWCCY 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 131
PspFI CCCAGC 1 cut(s) 161
PspN4I GGNNCC 1 cut(s) 133
PspPI GGNCC 1 cut(s) 131
PspPPI RGGWCCY 1 cut(s) 131
PsuI RGATCY 1 cut(s) 24
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 2 cut(s) 32, 57
Sau3AI GATC 1 cut(s) 24
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 1 cut(s) 333
SduI GDGCHC 1 cut(s) 336
SfcI CTRYAG 1 cut(s) 213
SinI GGWCC 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 1 cut(s) 251
SsiI CCGC 1 cut(s) 56
SspMI CTAG 1 cut(s) 143
StyI CCWWGG 1 cut(s) 292
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 2 cut(s) 48, 112
TaqI TCGA 3 cut(s) 61, 81, 267
TasI AATT 1 cut(s) 251
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 1 cut(s) 63
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TscAI CASTG 1 cut(s) 217
TseI GCWGC 1 cut(s) 31
TspDTI ATGAA 2 cut(s) 300, 342
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 131
VspI ATTAAT 1 cut(s) 225
XceI RCATGY 1 cut(s) 173
XspI CTAG 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.