Rw6G038970

glyoxalase I family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003849
Physical Location & Seq
Forward (+)
63042327 .. 63043119
793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G038970.1

Sequence Viewer

Length: 354 bp
ATGGCAGCGGCGTCGTTTAGGTGGATACTGCAATTGCACAAGGACGTCCCAAAAGCCGCTCGGTTCTACTCCGAAGGCTTGGATTTCACCGTCAACGTTTGCACTTTCCGCTGGGCCGAGCTTCAGTCCGGCCCACTCAAACTTGCCCTCATGCAATCCCCCAATGACCATGCCATGCAGAAGGGCTACTCTTCCCTTGTATCTTTCACAGTGTTGGACATTAACCAAACGGTGACGAAGCTAATGGCATTAGGAGCTGAGCTAGATGGTCCTATCAAATACGAAATCCATGGGAAGGTTGCTGCCCTGCGATGTATTGATGGGCACATGTTAGGCCTCTATGAACCAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

117

Amino Acids

12.97

Weight (kDa)

6.89

Isoelectric Point (pI)

48.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 8 - 99 3.8e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 48
AccBSI CCGCTC 1 cut(s) 59
AciI CCGC 3 cut(s) 8, 57, 109
AclI AACGTT 1 cut(s) 96
AcuI CTGAAG 1 cut(s) 107
AcyI GRCGYC 2 cut(s) 11, 45
AfiI CCNNNNNNNGG 1 cut(s) 295
AflIII ACRYGT 1 cut(s) 327
AluBI AGCT 4 cut(s) 121, 241, 257, 262
AluI AGCT 4 cut(s) 121, 241, 257, 262
AoxI GGCC 3 cut(s) 114, 130, 334
ApeKI GCWGC 2 cut(s) 5, 302
AspS9I GGNCC 3 cut(s) 114, 131, 269
AsuHPI GGTGA 2 cut(s) 79, 244
AvaII GGWCC 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 17, 289
BccI CCATC 2 cut(s) 260, 314
BcgI CGANNNNNNTGC 1 cut(s) 28
BciVI GTATCC 1 cut(s) 18
BfaI CTAG 1 cut(s) 263
BfuI GTATCC 1 cut(s) 18
BisI GCNGC 4 cut(s) 6, 9, 57, 303
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 4 cut(s) 7, 10, 58, 304
Bme18I GGWCC 1 cut(s) 269
BmgT120I GGNCC 3 cut(s) 114, 131, 269
Bpu1102I GCTNAGC 1 cut(s) 258
BsaHI GRCGYC 2 cut(s) 11, 45
BsaJI CCNNGG 1 cut(s) 289
Bsc4I CCNNNNNNNGG 1 cut(s) 295
BseDI CCNNGG 1 cut(s) 289
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMII CTCAG 1 cut(s) 249
BseSI GKGCMC 1 cut(s) 327
BseXI GCAGC 2 cut(s) 17, 289
BseYI CCCAGC 1 cut(s) 111
BshFI GGCC 3 cut(s) 116, 132, 336
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 32
BsnI GGCC 3 cut(s) 116, 132, 336
Bsp1286I GDGCHC 1 cut(s) 327
Bsp1720I GCTNAGC 1 cut(s) 258
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 3 cut(s) 8, 57, 109
BspANI GGCC 3 cut(s) 116, 132, 336
BspCNI CTCAG 1 cut(s) 250
BsrBI CCGCTC 1 cut(s) 59
BssECI CCNNGG 1 cut(s) 289
BssNI GRCGYC 2 cut(s) 11, 45
BssT1I CCWWGG 1 cut(s) 289
Bst4CI ACNGT 3 cut(s) 91, 211, 232
Bst6I CTCTTC 1 cut(s) 196
BstACI GRCGYC 2 cut(s) 11, 45
BstDEI CTNAG 1 cut(s) 258
BstDSI CCRYGG 1 cut(s) 289
BstMWI GCNNNNNNNGC 2 cut(s) 108, 254
BstNSI RCATGY 1 cut(s) 331
BstSLI GKGCMC 1 cut(s) 327
BstV1I GCAGC 2 cut(s) 17, 289
BsuI GTATCC 1 cut(s) 18
BsuRI GGCC 3 cut(s) 116, 132, 336
BtgI CCRYGG 1 cut(s) 289
BtgZI GCGATG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 216
Cfr13I GGNCC 3 cut(s) 114, 131, 269
CviAII CATG 5 cut(s) 151, 170, 175, 290, 328
DdeI CTNAG 1 cut(s) 258
Eam1104I CTCTTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 289
Eco147I AGGCCT 1 cut(s) 336
Eco47I GGWCC 1 cut(s) 269
Eco57I CTGAAG 1 cut(s) 107
EcoT14I CCWWGG 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 289
FaeI CATG 5 cut(s) 154, 173, 178, 293, 331
FaiI YATR 6 cut(s) 152, 171, 176, 291, 329, 342
FaqI GGGAC 1 cut(s) 32
FatI CATG 5 cut(s) 150, 169, 174, 289, 327
Fnu4HI GCNGC 4 cut(s) 6, 9, 57, 303
Fsp4HI GCNGC 4 cut(s) 6, 9, 57, 303
FspBI CTAG 1 cut(s) 263
GluI GCNGC 4 cut(s) 6, 9, 57, 303
GsaI CCCAGC 1 cut(s) 115
HaeIII GGCC 3 cut(s) 116, 132, 336
HapII CCGG 1 cut(s) 129
Hin1I GRCGYC 2 cut(s) 11, 45
Hin1II CATG 5 cut(s) 154, 173, 178, 293, 331
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HpaII CCGG 1 cut(s) 129
HphI GGTGA 2 cut(s) 79, 244
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 73
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 3 cut(s) 68, 175, 289
HpyCH4III ACNGT 3 cut(s) 91, 211, 232
HpyCH4IV ACGT 2 cut(s) 45, 96
HpyCH4V TGCA 5 cut(s) 31, 37, 102, 154, 178
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 254
HpyF3I CTNAG 1 cut(s) 258
HpySE526I ACGT 2 cut(s) 45, 96
Hsp92I GRCGYC 2 cut(s) 11, 45
Hsp92II CATG 5 cut(s) 154, 173, 178, 293, 331
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 3 cut(s) 97, 142, 320
Lsp1109I GCAGC 2 cut(s) 17, 289
MaeI CTAG 1 cut(s) 263
MaeII ACGT 2 cut(s) 45, 96
MaeIII GTNAC 1 cut(s) 232
MbiI CCGCTC 1 cut(s) 59
MboII GAAGA 1 cut(s) 183
MfeI CAATTG 1 cut(s) 32
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 2 cut(s) 158, 347
MseI TTAA 1 cut(s) 222
MspA1I CMGCKG 2 cut(s) 8, 111
MspI CCGG 1 cut(s) 129
MunI CAATTG 1 cut(s) 32
MwoI GCNNNNNNNGC 2 cut(s) 108, 254
NcoI CCATGG 1 cut(s) 289
NlaIII CATG 5 cut(s) 154, 173, 178, 293, 331
NmeAIII GCCGAG 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 232
NspI RCATGY 1 cut(s) 331
PceI AGGCCT 1 cut(s) 336
PciI ACATGT 1 cut(s) 327
PkrI GCNGC 4 cut(s) 7, 10, 58, 304
PscI ACATGT 1 cut(s) 327
Psp1406I AACGTT 1 cut(s) 96
PspFI CCCAGC 1 cut(s) 111
PspPI GGNCC 3 cut(s) 114, 131, 269
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 4 cut(s) 6, 9, 57, 303
Sau96I GGNCC 3 cut(s) 114, 131, 269
SduI GDGCHC 1 cut(s) 327
SetI ASST 8 cut(s) 23, 48, 99, 123, 243, 259, 264, 300
SinI GGWCC 1 cut(s) 269
Sse9I AATT 1 cut(s) 32
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 3 cut(s) 8, 57, 109
SspMI CTAG 1 cut(s) 263
StuI AGGCCT 1 cut(s) 336
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 3 cut(s) 91, 211, 232
TaiI ACGT 2 cut(s) 48, 99
TasI AATT 1 cut(s) 32
TauI GCSGC 2 cut(s) 11, 59
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 216
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 2 cut(s) 5, 302
Tsp45I GTSAC 1 cut(s) 232
TspRI CASTG 1 cut(s) 216
VpaK11BI GGWCC 1 cut(s) 269
XceI RCATGY 1 cut(s) 331
XspI CTAG 1 cut(s) 263
ZraI GACGTC 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.