Rroxscaffold_3G00260010

glyoxalase I family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003849
Physical Location & Seq
Forward (+)
54391102 .. 54391835
734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00260010.1

Sequence Viewer

Length: 354 bp
ATGGCAGCGGCGTCGTTTAGGTGGATACTGCAGCTGCACAAGGACGTGCCCAAAGCTGCTCGGTTCTACTCGGAAGGCTTGGACTTCACCGTGCACGTCTGTACTCTTCGCTGGGCTGAGCTTCAGTCTGGTCCACTCAAACTCGGCCTCATGCAATCCCCCAATGACCATGTAATGCAGAAGGGACACTCTTTGCTTCTGTCGTTCACAGTGACAGACATTAATCAAACAATGACGAAACTAATGGCATTAGGAGCTGAACTAGATGGTCCTATCAAATATGAAATCCATGGCAAGGTTGCAGCAATGCGCTGTCTTGATGGGCACATTTTAGGCCTCTATGAACCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

117

Amino Acids

13.01

Weight (kDa)

7.01

Isoelectric Point (pI)

52.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 8 - 102 5.4e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 1 cut(s) 103
AfiI CCNNNNNNNGG 1 cut(s) 295
AjiI CACGTC 2 cut(s) 46, 97
AluBI AGCT 4 cut(s) 34, 56, 121, 257
AluI AGCT 4 cut(s) 34, 56, 121, 257
Alw21I GWGCWC 1 cut(s) 96
Alw44I GTGCAC 1 cut(s) 92
AoxI GGCC 2 cut(s) 145, 334
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 5 cut(s) 5, 31, 34, 56, 302
AseI ATTAAT 1 cut(s) 222
AspLEI GCGC 1 cut(s) 312
AspS9I GGNCC 2 cut(s) 131, 269
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 2 cut(s) 131, 269
BaeGI GKGCMC 3 cut(s) 51, 96, 327
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 5 cut(s) 17, 21, 43, 43, 314
BccI CCATC 2 cut(s) 260, 314
BcgI CGANNNNNNTGC 1 cut(s) 28
BciVI GTATCC 1 cut(s) 18
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 1 cut(s) 29
BfuI GTATCC 1 cut(s) 18
BisI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 6 cut(s) 7, 10, 33, 36, 58, 304
Bme18I GGWCC 2 cut(s) 131, 269
BmgBI CACGTC 2 cut(s) 46, 97
BmgT120I GGNCC 2 cut(s) 131, 269
Bpu1102I GCTNAGC 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 289
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse3DI GCAATG 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 289
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMI GCAATG 1 cut(s) 312
BseMII CTCAG 1 cut(s) 108
BseSI GKGCMC 3 cut(s) 51, 96, 327
BseXI GCAGC 5 cut(s) 17, 21, 43, 43, 314
BseYI CCCAGC 1 cut(s) 111
BsgI GTGCAG 1 cut(s) 20
BshFI GGCC 2 cut(s) 147, 336
BsiHKAI GWGCWC 1 cut(s) 96
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 198
BsnI GGCC 2 cut(s) 147, 336
Bsp1286I GDGCHC 3 cut(s) 51, 96, 327
Bsp1720I GCTNAGC 1 cut(s) 117
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 1 cut(s) 8
BspANI GGCC 2 cut(s) 147, 336
BspCNI CTCAG 1 cut(s) 109
BspMAI CTGCAG 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 312
BssECI CCNNGG 1 cut(s) 289
BssNI GRCGYC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 289
Bst4CI ACNGT 2 cut(s) 91, 211
Bst6I CTCTTC 1 cut(s) 111
BstACI GRCGYC 1 cut(s) 11
BstDEI CTNAG 1 cut(s) 117
BstDSI CCRYGG 1 cut(s) 289
BstHHI GCGC 1 cut(s) 312
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstSFI CTRYAG 1 cut(s) 29
BstSLI GKGCMC 3 cut(s) 51, 96, 327
BstV1I GCAGC 5 cut(s) 17, 21, 43, 43, 314
BsuI GTATCC 1 cut(s) 18
BsuRI GGCC 2 cut(s) 147, 336
BtgI CCRYGG 1 cut(s) 289
BtrI CACGTC 2 cut(s) 46, 97
BtsIMutI CAGTG 1 cut(s) 216
CfoI GCGC 1 cut(s) 312
Cfr13I GGNCC 2 cut(s) 131, 269
Csp6I GTAC 1 cut(s) 102
CviAII CATG 3 cut(s) 151, 170, 290
CviJI RGCY 8 cut(s) 34, 56, 78, 116, 121, 147, 257, 336
CviKI_1 RGCY 8 cut(s) 34, 56, 78, 116, 121, 147, 257, 336
CviQI GTAC 1 cut(s) 102
DdeI CTNAG 1 cut(s) 117
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 289
Eco147I AGGCCT 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 131, 269
Eco57I CTGAAG 1 cut(s) 107
EcoT14I CCWWGG 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 289
FaeI CATG 3 cut(s) 154, 173, 293
FaiI YATR 6 cut(s) 152, 171, 282, 291, 342, 352
FaqI GGGAC 1 cut(s) 198
FatI CATG 3 cut(s) 150, 169, 289
Fnu4HI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
Fsp4HI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
FspBI CTAG 1 cut(s) 263
GlaI GCGC 1 cut(s) 311
GluI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
GsaI CCCAGC 1 cut(s) 115
HaeIII GGCC 2 cut(s) 147, 336
HhaI GCGC 1 cut(s) 312
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 3 cut(s) 154, 173, 293
Hin6I GCGC 1 cut(s) 310
HinP1I GCGC 1 cut(s) 310
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 3 cut(s) 94, 134, 207
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 3 cut(s) 94, 134, 207
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 2 cut(s) 68, 175
HpyCH4III ACNGT 2 cut(s) 91, 211
HpyCH4IV ACGT 2 cut(s) 45, 96
HpyCH4V TGCA 7 cut(s) 31, 37, 94, 154, 178, 302, 350
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 1 cut(s) 117
HpySE526I ACGT 2 cut(s) 45, 96
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 3 cut(s) 154, 173, 293
HspAI GCGC 1 cut(s) 310
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 2 cut(s) 97, 114
Lsp1109I GCAGC 5 cut(s) 17, 21, 43, 43, 314
MaeI CTAG 1 cut(s) 263
MaeII ACGT 2 cut(s) 45, 96
MaeIII GTNAC 1 cut(s) 211
MboII GAAGA 1 cut(s) 98
MhlI GDGCHC 3 cut(s) 51, 96, 327
MnlI CCTC 2 cut(s) 158, 347
MseI TTAA 1 cut(s) 222
MspA1I CMGCKG 2 cut(s) 8, 34
MwoI GCNNNNNNNGC 1 cut(s) 254
NcoI CCATGG 1 cut(s) 289
NlaIII CATG 3 cut(s) 154, 173, 293
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 211
PceI AGGCCT 1 cut(s) 336
PkrI GCNGC 6 cut(s) 7, 10, 33, 36, 58, 304
PshBI ATTAAT 1 cut(s) 222
PspFI CCCAGC 1 cut(s) 111
PspPI GGNCC 2 cut(s) 131, 269
PstI CTGCAG 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 34
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
Sau96I GGNCC 2 cut(s) 131, 269
SduI GDGCHC 3 cut(s) 51, 96, 327
SetI ASST 9 cut(s) 23, 36, 48, 58, 99, 123, 259, 300, 349
SfcI CTRYAG 1 cut(s) 29
SinI GGWCC 2 cut(s) 131, 269
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 1 cut(s) 8
SspMI CTAG 1 cut(s) 263
StuI AGGCCT 1 cut(s) 336
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 2 cut(s) 91, 211
TaiI ACGT 2 cut(s) 48, 99
TatI WGTACW 1 cut(s) 101
TauI GCSGC 1 cut(s) 11
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 216
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 5 cut(s) 5, 31, 34, 56, 302
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 1 cut(s) 297
TspRI CASTG 1 cut(s) 216
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 2 cut(s) 131, 269
VspI ATTAAT 1 cut(s) 222
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.