MD15G1108200.v1.1

glyoxalase I family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003849
Physical Location & Seq
Reverse (-)
7548790 .. 7549625
836 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1108200.v1.1.491

Sequence Viewer

Length: 354 bp
ATGGCAGCGGCGTCGTTTAGGTGGCTACTGCAGCTGCACAAGGACGTGCCAAAAGCCGCTCGGTTTTACGCCGAAGGGTTGGACTTCACCGTCAATGTCTGCACTCTTCGATGGGCGGAGCTCCAGTCCGGCCCGCTCAAGCTTTCCCTCATGCAATCCCCCAAGGATCATGTGATGCAGAGCGGAAACTCTTCACTTTTGTCCTTCACGGTGCCAGACATTAATCAAACGGTGACCAAGTTGATGGCGTTGGGAGCTGAACTCGATGGCCCTATCAAATATGAAGTTCACGGCAAGGTTGCAGCCATGCGATGTATTGACGGGCACATGCTAGGCCTCTATGAACCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

118

Amino Acids

12.89

Weight (kDa)

6.89

Isoelectric Point (pI)

49.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 11 - 102 2.5e-06 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 211
AccBSI CCGCTC 3 cut(s) 59, 136, 183
AciI CCGC 5 cut(s) 8, 57, 116, 134, 183
AclWI GGATC 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 11
AjiI CACGTC 1 cut(s) 46
AluBI AGCT 4 cut(s) 34, 121, 142, 257
AluI AGCT 4 cut(s) 34, 121, 142, 257
Alw21I GWGCWC 1 cut(s) 123
AlwI GGATC 1 cut(s) 174
AoxI GGCC 3 cut(s) 130, 268, 334
ApeKI GCWGC 4 cut(s) 5, 31, 34, 302
AseI ATTAAT 1 cut(s) 222
Asp700I GAANNNNTTC 1 cut(s) 190
AspS9I GGNCC 2 cut(s) 131, 269
AsuHPI GGTGA 2 cut(s) 79, 244
BaeGI GKGCMC 1 cut(s) 327
BanI GGYRCC 1 cut(s) 211
BanII GRGCYC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 4 cut(s) 17, 21, 43, 314
BccI CCATC 3 cut(s) 105, 238, 260
BceAI ACGGC 1 cut(s) 307
BcgI CGANNNNNNTGC 1 cut(s) 28
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 29
BisI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
BlsI GCNGC 6 cut(s) 7, 10, 33, 36, 58, 304
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 131, 269
BmiI GGNNCC 1 cut(s) 213
BmsI GCATC 1 cut(s) 165
BplI GAGNNNNNCTC 2 cut(s) 246, 278
BpmI CTGGAG 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 122
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 162
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bse1I ACTGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 162
BseNI ACTGG 1 cut(s) 124
BseSI GKGCMC 1 cut(s) 327
BseXI GCAGC 4 cut(s) 17, 21, 43, 314
BsgI GTGCAG 2 cut(s) 20, 85
BshFI GGCC 3 cut(s) 132, 270, 336
BshNI GGYRCC 1 cut(s) 211
BsiHKAI GWGCWC 1 cut(s) 123
BsiSI CCGG 1 cut(s) 129
BsnI GGCC 3 cut(s) 132, 270, 336
Bsp1286I GDGCHC 2 cut(s) 123, 327
Bsp143I GATC 1 cut(s) 166
BspACI CCGC 5 cut(s) 8, 57, 116, 134, 183
BspANI GGCC 3 cut(s) 132, 270, 336
BspLI GGNNCC 1 cut(s) 213
BspMAI CTGCAG 1 cut(s) 33
BspPI GGATC 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 211
BsrBI CCGCTC 3 cut(s) 59, 136, 183
BsrI ACTGG 1 cut(s) 124
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 1 cut(s) 166
BssNI GRCGYC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 162
Bst4CI ACNGT 3 cut(s) 91, 211, 232
Bst6I CTCTTC 2 cut(s) 111, 196
BstACI GRCGYC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 134
BstDEI CTNAG 1 cut(s) 351
BstEII GGTNACC 1 cut(s) 232
BstKTI GATC 1 cut(s) 169
BstMBI GATC 1 cut(s) 166
BstMWI GCNNNNNNNGC 2 cut(s) 31, 254
BstNSI RCATGY 1 cut(s) 331
BstPI GGTNACC 1 cut(s) 232
BstSFI CTRYAG 1 cut(s) 29
BstSLI GKGCMC 1 cut(s) 327
BstV1I GCAGC 4 cut(s) 17, 21, 43, 314
BstXI CCANNNNNNTGG 1 cut(s) 244
BsuRI GGCC 3 cut(s) 132, 270, 336
BtgZI GCGATG 1 cut(s) 325
BtrI CACGTC 1 cut(s) 46
Cac8I GCNNGC 1 cut(s) 134
Cfr13I GGNCC 2 cut(s) 131, 269
CviAII CATG 4 cut(s) 151, 170, 307, 328
DdeI CTNAG 1 cut(s) 351
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
Eam1104I CTCTTC 2 cut(s) 111, 196
EarI CTCTTC 2 cut(s) 111, 196
EciI GGCGGA 1 cut(s) 131
Ecl136II GAGCTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 162
Eco147I AGGCCT 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 121
Eco91I GGTNACC 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 121
EcoO65I GGTNACC 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 4 cut(s) 154, 173, 310, 331
FaiI YATR 6 cut(s) 152, 171, 282, 308, 329, 342
FatI CATG 4 cut(s) 150, 169, 306, 327
FauI CCCGC 1 cut(s) 141
Fnu4HI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
FspBI CTAG 1 cut(s) 332
GluI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 3 cut(s) 132, 270, 336
HapII CCGG 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 4 cut(s) 154, 173, 310, 331
HindIII AAGCTT 1 cut(s) 140
HpaII CCGG 1 cut(s) 129
HphI GGTGA 2 cut(s) 79, 244
Hpy166II GTNNAC 1 cut(s) 289
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 2 cut(s) 68, 214
HpyCH4III ACNGT 3 cut(s) 91, 211, 232
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 6 cut(s) 31, 37, 102, 154, 178, 302
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 254
HpyF3I CTNAG 1 cut(s) 351
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 4 cut(s) 154, 173, 310, 331
Kzo9I GATC 1 cut(s) 166
LmnI GCTCC 3 cut(s) 118, 126, 254
LpnPI CCDG 3 cut(s) 137, 142, 228
Lsp1109I GCAGC 4 cut(s) 17, 21, 43, 314
LweI GCATC 1 cut(s) 165
MaeI CTAG 1 cut(s) 332
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 232
MalI GATC 1 cut(s) 168
MbiI CCGCTC 3 cut(s) 59, 136, 183
MboI GATC 1 cut(s) 166
MboII GAAGA 2 cut(s) 98, 183
MhlI GDGCHC 2 cut(s) 123, 327
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 2 cut(s) 158, 347
MroXI GAANNNNTTC 1 cut(s) 190
MseI TTAA 1 cut(s) 222
MspA1I CMGCKG 2 cut(s) 8, 34
MspI CCGG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 31, 254
NdeII GATC 1 cut(s) 166
NlaIII CATG 4 cut(s) 154, 173, 310, 331
NlaIV GGNNCC 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 232
NspI RCATGY 1 cut(s) 331
PceI AGGCCT 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 190
PkrI GCNGC 6 cut(s) 7, 10, 33, 36, 58, 304
PshBI ATTAAT 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 123
PspEI GGTNACC 1 cut(s) 232
PspN4I GGNNCC 1 cut(s) 213
PspPI GGNCC 2 cut(s) 131, 269
PstI CTGCAG 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 34
SacI GAGCTC 1 cut(s) 123
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
Sau3AI GATC 1 cut(s) 166
Sau96I GGNCC 2 cut(s) 131, 269
SduI GDGCHC 2 cut(s) 123, 327
SetI ASST 8 cut(s) 23, 36, 48, 123, 144, 259, 300, 349
SfaNI GCATC 1 cut(s) 165
SfcI CTRYAG 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 5 cut(s) 8, 57, 116, 134, 183
SspMI CTAG 1 cut(s) 332
SstI GAGCTC 1 cut(s) 123
StuI AGGCCT 1 cut(s) 336
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 3 cut(s) 91, 211, 232
TaiI ACGT 1 cut(s) 48
TaqI TCGA 2 cut(s) 109, 264
TauI GCSGC 2 cut(s) 11, 59
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 4 cut(s) 5, 31, 34, 302
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 1 cut(s) 297
VspI ATTAAT 1 cut(s) 222
XceI RCATGY 1 cut(s) 331
XmnI GAANNNNTTC 1 cut(s) 190
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.