FvH4_5g03750

glyoxalase I family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003849
Physical Location & Seq
Forward (+)
2205196 .. 2206686
1491 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03750.t1

Sequence Viewer

Length: 354 bp
ATGGCAGCGGCGTCGTTTAGGTGGATACTGCAGCTGCACAAGGACGTGCCCAAAGCTGCTCGCTTCTACTCGGAAGGCTTGGATTTCACCGTGCACGTCTGCACTCTTCGCTGGGCCGAGCTCCAGTCAGGTCCCCTCAAACTCGGCCTCATGCAATCCCCCAATGACCATGTAATGCAGAAGGGGCACTCTTCGCTTCTGTCGTTCACAGTGACAGACATTAATGAAACGACGAGGAAACTAATGGCATTAGGAGCTGAACTTGATGGTCCTATCAAATATGAAATACATGGCAAGGTTGCGGCAATGCGCTGTCTTGATGGGCACGTTTTAGGCCTCTATGAACCGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

118

Amino Acids

12.99

Weight (kDa)

7.01

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 8 - 102 6.1e-08 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 8, 302
AcyI GRCGYC 1 cut(s) 11
AjiI CACGTC 2 cut(s) 46, 97
AluBI AGCT 4 cut(s) 34, 56, 121, 257
AluI AGCT 4 cut(s) 34, 56, 121, 257
Alw21I GWGCWC 2 cut(s) 96, 123
Alw44I GTGCAC 1 cut(s) 92
AoxI GGCC 3 cut(s) 114, 145, 334
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 4 cut(s) 5, 31, 34, 56
AseI ATTAAT 1 cut(s) 222
AspLEI GCGC 1 cut(s) 312
AspS9I GGNCC 3 cut(s) 114, 131, 269
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 2 cut(s) 131, 269
BaeGI GKGCMC 4 cut(s) 51, 96, 189, 327
BanII GRGCYC 1 cut(s) 123
Bbv12I GWGCWC 2 cut(s) 96, 123
BbvI GCAGC 4 cut(s) 17, 21, 43, 43
BccI CCATC 2 cut(s) 260, 314
BcgI CGANNNNNNTGC 1 cut(s) 28
BciVI GTATCC 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 29
BfuI GTATCC 1 cut(s) 18
BisI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
BlsI GCNGC 6 cut(s) 7, 10, 33, 36, 58, 304
Bme18I GGWCC 2 cut(s) 131, 269
BmgBI CACGTC 2 cut(s) 46, 97
BmgT120I GGNCC 3 cut(s) 114, 131, 269
BmiI GGNNCC 1 cut(s) 133
BpmI CTGGAG 1 cut(s) 107
BsaHI GRCGYC 1 cut(s) 11
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 1 cut(s) 124
Bse3DI GCAATG 1 cut(s) 312
BseMI GCAATG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 124
BseSI GKGCMC 4 cut(s) 51, 96, 189, 327
BseXI GCAGC 4 cut(s) 17, 21, 43, 43
BseYI CCCAGC 1 cut(s) 111
BsgI GTGCAG 2 cut(s) 20, 85
BshFI GGCC 3 cut(s) 116, 147, 336
BsiHKAI GWGCWC 2 cut(s) 96, 123
BsiSI CCGG 1 cut(s) 347
BslFI GGGAC 1 cut(s) 117
BsmFI GGGAC 1 cut(s) 117
BsnI GGCC 3 cut(s) 116, 147, 336
Bsp1286I GDGCHC 5 cut(s) 51, 96, 123, 189, 327
BspACI CCGC 2 cut(s) 8, 302
BspANI GGCC 3 cut(s) 116, 147, 336
BspLI GGNNCC 1 cut(s) 133
BspMAI CTGCAG 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 312
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 1 cut(s) 124
BssAI RCCGGY 1 cut(s) 346
BssNI GRCGYC 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 91, 211
Bst6I CTCTTC 2 cut(s) 111, 196
BstACI GRCGYC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 61
BstHHI GCGC 1 cut(s) 312
BstMWI GCNNNNNNNGC 4 cut(s) 108, 184, 193, 254
BstSFI CTRYAG 1 cut(s) 29
BstSLI GKGCMC 4 cut(s) 51, 96, 189, 327
BstV1I GCAGC 4 cut(s) 17, 21, 43, 43
BsuI GTATCC 1 cut(s) 18
BsuRI GGCC 3 cut(s) 116, 147, 336
BtrI CACGTC 2 cut(s) 46, 97
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 1 cut(s) 61
CfoI GCGC 1 cut(s) 312
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 3 cut(s) 114, 131, 269
CviAII CATG 3 cut(s) 151, 170, 290
CviJI RGCY 8 cut(s) 34, 56, 78, 116, 121, 147, 257, 336
CviKI_1 RGCY 8 cut(s) 34, 56, 78, 116, 121, 147, 257, 336
Eam1104I CTCTTC 2 cut(s) 111, 196
EarI CTCTTC 2 cut(s) 111, 196
Ecl136II GAGCTC 1 cut(s) 121
Eco147I AGGCCT 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 123
Eco47I GGWCC 2 cut(s) 131, 269
Eco53kI GAGCTC 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 131
EcoT38I GRGCYC 1 cut(s) 123
FaeI CATG 3 cut(s) 154, 173, 293
FaiI YATR 6 cut(s) 152, 171, 282, 291, 342, 352
FaqI GGGAC 1 cut(s) 117
FatI CATG 3 cut(s) 150, 169, 289
Fnu4HI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
GlaI GCGC 1 cut(s) 311
GluI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
GsaI CCCAGC 1 cut(s) 115
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 3 cut(s) 116, 147, 336
HapII CCGG 1 cut(s) 347
HhaI GCGC 1 cut(s) 312
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 3 cut(s) 154, 173, 293
Hin6I GCGC 1 cut(s) 310
HinP1I GCGC 1 cut(s) 310
HpaII CCGG 1 cut(s) 347
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 2 cut(s) 94, 207
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 2 cut(s) 94, 207
Hpy99I CGWCG 2 cut(s) 16, 235
HpyAV CCTTC 2 cut(s) 68, 175
HpyCH4III ACNGT 2 cut(s) 91, 211
HpyCH4IV ACGT 3 cut(s) 45, 96, 327
HpyCH4V TGCA 6 cut(s) 31, 37, 94, 102, 154, 178
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 184, 193, 254
HpySE526I ACGT 3 cut(s) 45, 96, 327
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 3 cut(s) 154, 173, 293
HspAI GCGC 1 cut(s) 310
LmnI GCTCC 2 cut(s) 126, 254
LpnPI CCDG 3 cut(s) 97, 114, 137
Lsp1109I GCAGC 4 cut(s) 17, 21, 43, 43
MaeII ACGT 3 cut(s) 45, 96, 327
MaeIII GTNAC 1 cut(s) 211
MboII GAAGA 2 cut(s) 98, 183
MhlI GDGCHC 5 cut(s) 51, 96, 123, 189, 327
MnlI CCTC 4 cut(s) 146, 158, 228, 347
MseI TTAA 1 cut(s) 222
MspA1I CMGCKG 2 cut(s) 8, 34
MspI CCGG 1 cut(s) 347
MwoI GCNNNNNNNGC 4 cut(s) 108, 184, 193, 254
NlaIII CATG 3 cut(s) 154, 173, 293
NlaIV GGNNCC 1 cut(s) 133
NmeAIII GCCGAG 2 cut(s) 123, 142
NmuCI GTSAC 1 cut(s) 211
PceI AGGCCT 1 cut(s) 336
PcsI WCGNNNNNNNCGW 1 cut(s) 200
PkrI GCNGC 6 cut(s) 7, 10, 33, 36, 58, 304
PpuMI RGGWCCY 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 123
Psp5II RGGWCCY 1 cut(s) 131
PspFI CCCAGC 1 cut(s) 111
PspN4I GGNNCC 1 cut(s) 133
PspPI GGNCC 3 cut(s) 114, 131, 269
PspPPI RGGWCCY 1 cut(s) 131
PstI CTGCAG 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 34
SacI GAGCTC 1 cut(s) 123
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 6 cut(s) 6, 9, 32, 35, 57, 303
Sau96I GGNCC 3 cut(s) 114, 131, 269
SduI GDGCHC 5 cut(s) 51, 96, 123, 189, 327
SfcI CTRYAG 1 cut(s) 29
SinI GGWCC 2 cut(s) 131, 269
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 2 cut(s) 8, 302
SstI GAGCTC 1 cut(s) 123
StuI AGGCCT 1 cut(s) 336
TaaI ACNGT 2 cut(s) 91, 211
TaiI ACGT 3 cut(s) 48, 99, 330
TauI GCSGC 2 cut(s) 11, 305
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 216
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 4 cut(s) 5, 31, 34, 56
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 2 cut(s) 240, 297
TspRI CASTG 1 cut(s) 216
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 2 cut(s) 131, 269
VspI ATTAAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.