MD08G1130100.v1.1

glyoxalase I family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003849
Physical Location & Seq
Reverse (-)
12248292 .. 12253011
4720 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1130100.v1.1.491

Sequence Viewer

Length: 489 bp
ATGGCAGCGGAGTCGTTTAGGTGGCTTCTGCAGCTGCACAAGGAAGTGCCAAAAGCCTCCCGGTTCAACACCGAAGGGTTGGACTTCACCGTTAATGTCTGCACTATTCGATGGGCGGAGCTACAGTTCGGCCCACTCAAGTTTTCCCTCGTACAATCCCCCAATTTTCCATCGGACCAGTCAACTTTGAGGCCATTTTCCGACCATCTCTGGCAATCATTGGGCTTCCAAATAGTAGCTATGCAGCTGCACAAGGACGTGCCAAAAGGCGCCCGGTTCAACTCCGAAGGGTTGGACTTCGCCGTTAATGTCTGCACTATTCGATGGGCGGAGCTACAGTTGGGCACACCAGATGGGTGCTCGATCCCCCAAAATGGAAAGTCTTCACTTTTGTTCTTGACAGTGCCAGACATTAATCAAACAGTGACGAATTTAATGGCGTTAGGAGGAGCTGAACTTGATGGCCCTATCAAATTTAAGTCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

163

Amino Acids

18.01

Weight (kDa)

6.27

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 269
AciI CCGC 3 cut(s) 8, 116, 329
AclWI GGATC 1 cut(s) 358
AcsI RAATTY 2 cut(s) 430, 473
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 1 cut(s) 153
AfiI CCNNNNNNNGG 1 cut(s) 374
AgsI TTSAA 2 cut(s) 67, 280
AjiI CACGTC 1 cut(s) 259
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
AluBI AGCT 6 cut(s) 34, 121, 239, 247, 334, 452
AluI AGCT 6 cut(s) 34, 121, 239, 247, 334, 452
Alw21I GWGCWC 1 cut(s) 362
AlwI GGATC 1 cut(s) 358
AoxI GGCC 3 cut(s) 130, 191, 463
ApeKI GCWGC 5 cut(s) 5, 31, 34, 244, 247
ApoI RAATTY 2 cut(s) 430, 473
AseI ATTAAT 1 cut(s) 414
Asp700I GAANNNNTTC 1 cut(s) 382
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 3 cut(s) 131, 175, 464
AsuC2I CCSGG 2 cut(s) 61, 274
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 347
BanI GGYRCC 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 362
BbvI GCAGC 5 cut(s) 17, 21, 43, 234, 256
BccI CCATC 6 cut(s) 105, 178, 213, 318, 347, 455
BceAI ACGGC 1 cut(s) 287
BcgI CGANNNNNNTGC 1 cut(s) 28
BcnI CCSGG 2 cut(s) 61, 274
BfmI CTRYAG 3 cut(s) 29, 122, 335
BfoI RGCGCY 1 cut(s) 273
BisI GCNGC 5 cut(s) 6, 32, 35, 245, 248
BlsI GCNGC 5 cut(s) 7, 33, 36, 246, 249
Bme1390I CCNGG 2 cut(s) 61, 274
Bme18I GGWCC 1 cut(s) 175
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 3 cut(s) 131, 175, 464
BmiI GGNNCC 1 cut(s) 271
BmrFI CCNGG 2 cut(s) 61, 274
BpiI GAAGAC 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 122
BpuMI CCSGG 2 cut(s) 61, 274
BsaHI GRCGYC 1 cut(s) 270
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 323, 353
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse1I ACTGG 1 cut(s) 178
BseLI CCNNNNNNNGG 1 cut(s) 374
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 1 cut(s) 462
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 5 cut(s) 17, 21, 43, 234, 256
BsgI GTGCAG 4 cut(s) 20, 85, 233, 298
BshFI GGCC 3 cut(s) 132, 193, 465
BshNI GGYRCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 362
BsiSI CCGG 2 cut(s) 61, 274
BslI CCNNNNNNNGG 1 cut(s) 374
BsnI GGCC 3 cut(s) 132, 193, 465
Bsp1286I GDGCHC 2 cut(s) 347, 362
Bsp143I GATC 1 cut(s) 363
BspACI CCGC 3 cut(s) 8, 116, 329
BspANI GGCC 3 cut(s) 132, 193, 465
BspLI GGNNCC 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 33
BspPI GGATC 1 cut(s) 358
BspT107I GGYRCC 1 cut(s) 269
BsrI ACTGG 1 cut(s) 178
BssMI GATC 1 cut(s) 363
BssNI GRCGYC 1 cut(s) 270
Bst4CI ACNGT 5 cut(s) 91, 126, 339, 403, 424
BstACI GRCGYC 1 cut(s) 270
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 1 cut(s) 366
BstMBI GATC 1 cut(s) 363
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 59, 272
BstSFI CTRYAG 3 cut(s) 29, 122, 335
BstSLI GKGCMC 1 cut(s) 347
BstV1I GCAGC 5 cut(s) 17, 21, 43, 234, 256
BstV2I GAAGAC 1 cut(s) 375
BsuRI GGCC 3 cut(s) 132, 193, 465
BtrI CACGTC 1 cut(s) 259
BtsIMutI CAGTG 2 cut(s) 408, 429
CfoI GCGC 1 cut(s) 272
Cfr13I GGNCC 3 cut(s) 131, 175, 464
Csp6I GTAC 1 cut(s) 152
CviAII CATG 1 cut(s) 486
CviQI GTAC 1 cut(s) 152
DinI GGCGCC 1 cut(s) 271
DpnI GATC 1 cut(s) 365
DpnII GATC 1 cut(s) 363
EciI GGCGGA 2 cut(s) 131, 344
Eco47I GGWCC 1 cut(s) 175
EgeI GGCGCC 1 cut(s) 271
EheI GGCGCC 1 cut(s) 271
FaeI CATG 1 cut(s) 489
FaiI YATR 2 cut(s) 242, 487
FatI CATG 1 cut(s) 485
Fnu4HI GCNGC 5 cut(s) 6, 32, 35, 245, 248
Fsp4HI GCNGC 5 cut(s) 6, 32, 35, 245, 248
GlaI GCGC 1 cut(s) 271
GluI GCNGC 5 cut(s) 6, 32, 35, 245, 248
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 3 cut(s) 132, 193, 465
HapII CCGG 2 cut(s) 61, 274
HhaI GCGC 1 cut(s) 272
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 1 cut(s) 489
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HincII GTYRAC 2 cut(s) 183, 483
HindII GTYRAC 2 cut(s) 183, 483
HinfI GANTC 1 cut(s) 11
HpaII CCGG 2 cut(s) 61, 274
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 2 cut(s) 183, 483
Hpy188I TCNGA 3 cut(s) 175, 202, 286
Hpy188III TCNNGA 1 cut(s) 397
Hpy8I GTNNAC 2 cut(s) 183, 483
HpyAV CCTTC 2 cut(s) 68, 281
HpyCH4III ACNGT 5 cut(s) 91, 126, 339, 403, 424
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 6 cut(s) 31, 37, 102, 244, 250, 315
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 1 cut(s) 489
HspAI GCGC 1 cut(s) 270
KasI GGCGCC 1 cut(s) 269
Kzo9I GATC 1 cut(s) 363
LmnI GCTCC 3 cut(s) 118, 331, 449
LpnPI CCDG 6 cut(s) 74, 191, 196, 287, 363, 420
Lsp1109I GCAGC 5 cut(s) 17, 21, 43, 234, 256
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 424
MalI GATC 1 cut(s) 365
MboI GATC 1 cut(s) 363
MboII GAAGA 1 cut(s) 375
MhlI GDGCHC 2 cut(s) 347, 362
MluCI AATT 3 cut(s) 163, 430, 473
Mly113I GGCGCC 1 cut(s) 270
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 3 cut(s) 60, 225, 273
MnlI CCTC 4 cut(s) 67, 158, 183, 440
MroXI GAANNNNTTC 1 cut(s) 382
MseI TTAA 5 cut(s) 93, 306, 414, 434, 477
MspA1I CMGCKG 3 cut(s) 8, 34, 247
MspI CCGG 2 cut(s) 61, 274
MspR9I CCNGG 2 cut(s) 61, 274
MwoI GCNNNNNNNGC 1 cut(s) 31
NarI GGCGCC 1 cut(s) 270
NciI CCSGG 2 cut(s) 61, 274
NdeII GATC 1 cut(s) 363
NlaIII CATG 1 cut(s) 489
NlaIV GGNNCC 1 cut(s) 271
NmuCI GTSAC 1 cut(s) 424
PdmI GAANNNNTTC 1 cut(s) 382
PkrI GCNGC 5 cut(s) 7, 33, 36, 246, 249
PleI GAGTC 1 cut(s) 19
PluTI GGCGCC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 19
PshBI ATTAAT 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 3 cut(s) 131, 175, 464
PstI CTGCAG 1 cut(s) 33
PvuII CAGCTG 2 cut(s) 34, 247
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
SaqAI TTAA 5 cut(s) 93, 306, 414, 434, 477
SatI GCNGC 5 cut(s) 6, 32, 35, 245, 248
Sau3AI GATC 1 cut(s) 363
Sau96I GGNCC 3 cut(s) 131, 175, 464
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 2 cut(s) 61, 274
SduI GDGCHC 2 cut(s) 347, 362
SetI ASST 8 cut(s) 23, 36, 123, 241, 249, 261, 336, 454
SfcI CTRYAG 3 cut(s) 29, 122, 335
SfoI GGCGCC 1 cut(s) 271
SinI GGWCC 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 3 cut(s) 163, 430, 473
SsiI CCGC 3 cut(s) 8, 116, 329
SspDI GGCGCC 1 cut(s) 269
StyD4I CCNGG 2 cut(s) 59, 272
TaaI ACNGT 5 cut(s) 91, 126, 339, 403, 424
TaiI ACGT 1 cut(s) 261
TaqI TCGA 3 cut(s) 109, 322, 362
TasI AATT 3 cut(s) 163, 430, 473
Tru1I TTAA 5 cut(s) 93, 306, 414, 434, 477
Tru9I TTAA 5 cut(s) 93, 306, 414, 434, 477
TscAI CASTG 2 cut(s) 408, 429
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 5 cut(s) 5, 31, 34, 244, 247
Tsp45I GTSAC 1 cut(s) 424
TspRI CASTG 2 cut(s) 408, 429
VpaK11BI GGWCC 1 cut(s) 175
VspI ATTAAT 1 cut(s) 414
XapI RAATTY 2 cut(s) 430, 473
XmnI GAANNNNTTC 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.