AT5G41650

glyoxalase I family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003849
Physical Location & Seq
Forward (+)
16654913 .. 16655860
948 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G41650.1

Sequence Viewer

Length: 354 bp
ATGGCGACGGCGTCGTTTAGGTGGATTCTGCAGCTTCACAGAGACGTACCAAAAGCTGCTCGATTCTATGCTCAAGGTCTCGACTTTTCCGTCAATGTCGTCACTTTACGCTGGGCTGAGCTCCATTCTGGTCCTATCAAGCTAGCTCTTATGCAATCTCCTAGCAATCATGTTGCTGAGAAGGGATACTCATCGCTTCTATCGTTCACAGTCACTGACATTAATACAACAGTGACTAAACTTATGGCTTTAGGAGCTGAATTAGATGGCACTATCAAGTATGAGATACATGGAAAGGTTGCGGCTATGAAATGCCCTGATGGTTACATGTTAGGCCTTTATGAGGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

117

Amino Acids

12.86

Weight (kDa)

7.89

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 9 - 111 1.9e-08 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AciI CCGC 1 cut(s) 302
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 1 cut(s) 343
AflIII ACRYGT 1 cut(s) 327
AluBI AGCT 6 cut(s) 34, 56, 121, 142, 146, 257
AluI AGCT 6 cut(s) 34, 56, 121, 142, 146, 257
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 2 cut(s) 36, 83
AlwNI CAGNNNCTG 1 cut(s) 215
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 3 cut(s) 31, 56, 347
AseI ATTAAT 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 131
AsuNHI GCTAGC 1 cut(s) 142
AvaII GGWCC 1 cut(s) 131
BanII GRGCYC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 3 cut(s) 43, 43, 334
BccI CCATC 2 cut(s) 260, 314
BceAI ACGGC 1 cut(s) 24
BciVI GTATCC 1 cut(s) 179
BcoDI GTCTC 2 cut(s) 36, 83
BfaI CTAG 2 cut(s) 143, 162
BfmI CTRYAG 1 cut(s) 29
BfuI GTATCC 1 cut(s) 179
BisI GCNGC 4 cut(s) 32, 57, 303, 348
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 4 cut(s) 33, 58, 304, 349
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 131
BmtI GCTAGC 1 cut(s) 146
Bpu1102I GCTNAGC 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 57
BsaBI GATNNNNATC 1 cut(s) 190
BsaHI GRCGYC 1 cut(s) 11
BsaI GGTCTC 1 cut(s) 83
Bsc4I CCNNNNNNNGG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 190
BseJI GATNNNNATC 1 cut(s) 190
BseLI CCNNNNNNNGG 1 cut(s) 343
BseMII CTCAG 2 cut(s) 108, 168
BseXI GCAGC 3 cut(s) 43, 43, 334
BseYI CCCAGC 1 cut(s) 111
BshFI GGCC 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 343
BsmAI GTCTC 2 cut(s) 36, 83
BsmBI CGTCTC 1 cut(s) 36
BsnI GGCC 1 cut(s) 336
Bso31I GGTCTC 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 123
Bsp1720I GCTNAGC 1 cut(s) 117
BspACI CCGC 1 cut(s) 302
BspANI GGCC 1 cut(s) 336
BspCNI CTCAG 2 cut(s) 109, 169
BspMAI CTGCAG 1 cut(s) 33
BspOI GCTAGC 1 cut(s) 146
BspTNI GGTCTC 1 cut(s) 83
BssNI GRCGYC 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 211, 232
BstACI GRCGYC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 2 cut(s) 117, 177
BstENI CCTNNNNNAGG 1 cut(s) 341
BstMAI GTCTC 2 cut(s) 36, 83
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNSI RCATGY 1 cut(s) 331
BstSFI CTRYAG 1 cut(s) 29
BstV1I GCAGC 3 cut(s) 43, 43, 334
BsuI GTATCC 1 cut(s) 179
BsuRI GGCC 1 cut(s) 336
BtgZI GCGATG 1 cut(s) 177
BtsIMutI CAGTG 2 cut(s) 213, 237
Cac8I GCNNGC 1 cut(s) 144
CaiI CAGNNNCTG 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 131
Csp6I GTAC 1 cut(s) 47
CviAII CATG 4 cut(s) 170, 290, 328, 351
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 2 cut(s) 117, 177
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
Ecl136II GAGCTC 1 cut(s) 121
Eco147I AGGCCT 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 131
Eco53kI GAGCTC 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 121
EcoNI CCTNNNNNAGG 1 cut(s) 341
EcoT38I GRGCYC 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 4 cut(s) 173, 293, 331, 354
FatI CATG 4 cut(s) 169, 289, 327, 350
Fnu4HI GCNGC 4 cut(s) 32, 57, 303, 348
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 4 cut(s) 32, 57, 303, 348
FspBI CTAG 2 cut(s) 143, 162
GluI GCNGC 4 cut(s) 32, 57, 303, 348
GsaI CCCAGC 1 cut(s) 115
HaeIII GGCC 1 cut(s) 336
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 4 cut(s) 173, 293, 331, 354
HinfI GANTC 2 cut(s) 25, 63
Hpy166II GTNNAC 1 cut(s) 207
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 1 cut(s) 207
Hpy99I CGWCG 2 cut(s) 10, 16
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 2 cut(s) 211, 232
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 3 cut(s) 31, 154, 350
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 2 cut(s) 117, 177
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 4 cut(s) 173, 293, 331, 354
LmnI GCTCC 2 cut(s) 126, 254
LpnPI CCDG 3 cut(s) 97, 114, 330
Lsp1109I GCAGC 3 cut(s) 43, 43, 334
MaeI CTAG 2 cut(s) 143, 162
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 4 cut(s) 100, 211, 232, 323
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 1 cut(s) 260
MnlI CCTC 1 cut(s) 337
MseI TTAA 1 cut(s) 222
MwoI GCNNNNNNNGC 1 cut(s) 254
NheI GCTAGC 1 cut(s) 142
NlaIII CATG 4 cut(s) 173, 293, 331, 354
NmuCI GTSAC 3 cut(s) 100, 211, 232
NspI RCATGY 1 cut(s) 331
PceI AGGCCT 1 cut(s) 336
PciI ACATGT 1 cut(s) 327
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 200
PfeI GAWTC 2 cut(s) 25, 63
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 4 cut(s) 33, 58, 304, 349
PscI ACATGT 1 cut(s) 327
PshBI ATTAAT 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 123
PspFI CCCAGC 1 cut(s) 111
PspPI GGNCC 1 cut(s) 131
PstI CTGCAG 1 cut(s) 33
PstNI CAGNNNCTG 1 cut(s) 215
PsyI GACNNNGTC 1 cut(s) 10
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
SacI GAGCTC 1 cut(s) 123
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 4 cut(s) 32, 57, 303, 348
Sau96I GGNCC 1 cut(s) 131
SduI GDGCHC 1 cut(s) 123
SfcI CTRYAG 1 cut(s) 29
SinI GGWCC 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 1 cut(s) 260
SseBI AGGCCT 1 cut(s) 336
SsiI CCGC 1 cut(s) 302
SspMI CTAG 2 cut(s) 143, 162
SstI GAGCTC 1 cut(s) 123
StuI AGGCCT 1 cut(s) 336
TaaI ACNGT 2 cut(s) 211, 232
TaiI ACGT 1 cut(s) 48
TaqI TCGA 2 cut(s) 61, 81
TasI AATT 1 cut(s) 260
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 2 cut(s) 25, 63
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 2 cut(s) 220, 237
TseFI GTSAC 3 cut(s) 100, 211, 232
TseI GCWGC 3 cut(s) 31, 56, 347
Tsp45I GTSAC 3 cut(s) 100, 211, 232
TspDTI ATGAA 1 cut(s) 323
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 2 cut(s) 220, 237
Tth111I GACNNNGTC 1 cut(s) 10
VpaK11BI GGWCC 1 cut(s) 131
VspI ATTAAT 1 cut(s) 222
XagI CCTNNNNNAGG 1 cut(s) 341
XceI RCATGY 1 cut(s) 331
XspI CTAG 2 cut(s) 143, 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.