Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0003849

Physical Location & Seq

Genomic Coordinates Forward (+)

1925165 .. 1927904

Gene Structure 2740 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0027300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGAACTTGAAAGACAACTACCGCATCGAGCTCCGCGCCGCTATTCGCCAGCTAACCGATCGTTGTCTCTACGCCGCGTCCAAATGGGCGGGAGAGCAACTGGTCGGCATCGAGCACGACCCGGCCAAGTTCACGCCGGCCAACACCAGATTCCAGCGCGGGAGCTCCAGCATTCGCCGGAGATTCCGCTCCAGTGACGTCATCTCGACGCCGATAGCCGGCGTGTCGTATGTGTCGACTCCGATTATGGAGGAGGACGAGATTGTGGACGGCGATTTTTACCTTCTGGCCAAGTCTTACTTTGATTGCAGAGAGTATAGAAGAGCTGCTCATGCTCTTCGTGATCAAGGTGGTAAAAAATCAGTCTTTTTGCGCTGCTATGCTCTTTACCTGGCTGGAGAGAAGCGGAAAGAGGAAGAATTGATGGAACTTGAGGGACCACTAGGAAAGAGCGACATTGTGAATCGTGAACTGGTTTCTATTGAGAGGGAGTTATCAACTTTTCGCAAAAATTGTACGATTGATCCTTTTGGACTGTACTTGTATGGCCTTGTGCTCAAAGAGAAAGGCAATGGGAACCTTGCTCGTACAGTTCTTGTGGAGTCTGTTAATAGCTACCCTTGGAACTGGAATGCCTGGTTAGAACTGCAGTCCTTGTGCACTACAGTTGACATCTTGAACAGCCTTGGTCTCAATAATCATTGGATGAAGGAGTTCTTTCTTGCCAATGTTTACCAAGAACTAAGGATGCACAAAGAATCCTTGTCAAAGTATGATTATCTACATGGCATTTTTGGTTTCAGTAATTACATTCAGGCTCAGATTGCAAAAGTCCAGTACAGTTTGAGGGAATTTGATCTAGTTGAAGGAATTTTTGAAGATCTTCTCAGGAATGACCCTTATCGAGTGGAGGACATGGATATGTATTCCAATGTGCTGTATGCAAAGGAATGTTCATCTGCACTGAGTTACCTTGCCCATAGAGTATTTATGACCGATAAATACAGACCTGAATCTTGTTGCATTATTGGGAATTATTACAGCTTAAAAGGGCTGCATGAGAAAGCAGTTCTGTACTTTAGGAGGGCACTCAAACTGGATAGAAATTATTTATCTGCTTGGACCCTTATGGGTCATGAATTTATTGAGATGAAAAACACACCAGCTGCTGTTGACGCTTATCGGCATGCTATAGATATAAATCCGAGTGATTATCGTGCTTGGTATGGATTAGGACAAGCTTATGAGATGATGGGTATGCCCTTTTATGCTCTTCATTACTTCCGGAAATCGGTATTTTTCCAGCCAAATGATTCTCGGTTATGGATTGCCATGGCTCATTGTTATCAAACTGATCAGCTAAATATGATTGATGAAGCAATGAAGTGTTACAAAAGGGCAGCAAATTGTAATGACAGGGAATCGATTGCCCTGAACCAGCTAGCTAAGTTAAATGCTGATCTTGGACGTACCGAAGAGGCAGTTTATTACCACAAGAAGGATCTGGAGAGGATGGAAGATGAAGAGAGGGAAGGACCAAATATGGTTGAAACTCTATTCTTTCTTGCTAAATACTTCAGAGAGCAGAAAAAGTTTGAAGAAGCAGAGGTCTACTGTACCCGTCTTATGGATTATACCGGCCCGGAAAAGGAAAGAGCGAAAAGTTTACTTAGAGGAATTAGAATGGAACAAACTGGTGCTCCTTCTATGGATATTGAGCGCTTTCCTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyoxalase

Protein Analysis

577

Amino Acids

67.44

Weight (kDa)

6.25

Isoelectric Point (pI)

42.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANAPC8	PF04049	7 - 276	1.7e-73	Anaphase promoting complex subunit 8 / Cdc23
TPR_11	PF13414	378 - 419	2.1e-08	TPR repeat
TPR_12	PF13424	476 - 536	2.4e-06	Tetratricopeptide repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003849)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64185 AT5G41650
fragaria_vesca	FvH4_5g03750 FvH4_5g03750 FvH4_5g27350 FvH4_5g27350
malus_domestica	MD07G1004700.v1.1 MD08G1128900.v1.1 MD08G1130100.v1.1 MD14G1117200.v1.1 MD15G1108200.v1.1
prunus_persica	Prupe.1G460500_v2.0.a1 Prupe.5G111900_v2.0.a1
pyrus_communis	pycom15g09890
rosa_chinensis	RchiOBHm_Chr5g0061641 RchiOBHm_Chr6g0304921 RchiOBHm_Chr7g0195901
rosa_laevigata	RLG00000004081 RLG00000010938
rosa_multiflora	Rmu_sc0000301.1_g000010 Rmu_sc0004222.1_g000003
rosa_roxburghii	Rroxscaffold_3G00260010 Rroxscaffold_7G00163340
rosa_rugosa	Rorug06G0336200 Rorug06G0336300 Rorug07G0027300
rosa_samantha	Rh6BG453900 Rh6CG461100 Rh7AG154900 Rh7BG156600 Rh7CG160400 Rh7DG155900
rosa_wichuraiana	Rw6G038970 Rw7G013240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	201
AccBSI	CCGCTC	1 cut(s)	189
AccI	GTMKAC	2 cut(s)	236, 1611
AccII	CGCG	3 cut(s)	36, 77, 159
AccIII	TCCGGA	1 cut(s)	1284
AciI	CCGC	8 cut(s)	22, 34, 39, 75, 89, 159, 187, 406
AclWI	GGATC	2 cut(s)	518, 1509
AcoI	YGGCCR	3 cut(s)	123, 138, 288
AcsI	RAATTY	3 cut(s)	851, 870, 1139
AcuI	CTGAAG	1 cut(s)	1561
AcyI	GRCGYC	2 cut(s)	198, 209
AfaI	GTAC	7 cut(s)	517, 539, 589, 839, 1076, 1471, 1618
AfeI	AGCGCT	1 cut(s)	1721
AfiI	CCNNNNNNNGG	5 cut(s)	218, 1291, 1498, 1627, 1648
AgsI	TTSAA	6 cut(s)	10, 679, 866, 878, 1550, 1598
AjnI	CCWGG	2 cut(s)	390, 635
AjuI	GAANNNNNNNTTGG	2 cut(s)	134, 166
Alw21I	GWGCWC	6 cut(s)	33, 117, 167, 558, 662, 1702
Alw26I	GTCTC	2 cut(s)	71, 695
Alw44I	GTGCAC	1 cut(s)	658
AlwI	GGATC	2 cut(s)	518, 1509
AlwNI	CAGNNNCTG	1 cut(s)	1169
Aor13HI	TCCGGA	1 cut(s)	1284
Aor51HI	AGCGCT	1 cut(s)	1721
AoxI	GGCC	5 cut(s)	123, 138, 288, 547, 1639
ApaLI	GTGCAC	1 cut(s)	658
ApeKI	GCWGC	5 cut(s)	326, 375, 1054, 1166, 1400
ApoI	RAATTY	3 cut(s)	851, 870, 1139
Asp700I	GAANNNNTTC	2 cut(s)	713, 882
AspLEI	GCGC	4 cut(s)	38, 159, 375, 1722
AspS9I	GGNCC	4 cut(s)	437, 1122, 1535, 1640
AsuC2I	CCSGG	2 cut(s)	122, 1643
AsuNHI	GCTAGC	1 cut(s)	1441
AvaII	GGWCC	3 cut(s)	437, 1122, 1535
BaeGI	GKGCMC	2 cut(s)	662, 1090
BalI	TGGCCA	1 cut(s)	290
BanII	GRGCYC	2 cut(s)	33, 167
Bbv12I	GWGCWC	6 cut(s)	33, 117, 167, 558, 662, 1702
BbvI	GCAGC	5 cut(s)	313, 362, 1041, 1153, 1412
BccI	CCATC	3 cut(s)	418, 1246, 1507
BceAI	ACGGC	1 cut(s)	286
BciT130I	CCWGG	2 cut(s)	392, 637
BclI	TGATCA	2 cut(s)	343, 1354
BcnI	CCSGG	2 cut(s)	122, 1643
BcoDI	GTCTC	2 cut(s)	71, 695
BfaI	CTAG	3 cut(s)	443, 860, 1442
BfmI	CTRYAG	3 cut(s)	647, 663, 1191
BfoI	RGCGCY	1 cut(s)	1723
BglII	AGATCT	1 cut(s)	880
BisI	GCNGC	7 cut(s)	39, 75, 327, 376, 1055, 1167, 1401
BlsI	GCNGC	7 cut(s)	40, 76, 328, 377, 1056, 1168, 1402
Bme1390I	CCNGG	4 cut(s)	122, 392, 637, 1643
Bme18I	GGWCC	3 cut(s)	437, 1122, 1535
BmgT120I	GGNCC	4 cut(s)	437, 1122, 1535, 1640
BmiI	GGNNCC	3 cut(s)	438, 578, 1124
BmrFI	CCNGG	4 cut(s)	122, 392, 637, 1643
BmsI	GCATC	3 cut(s)	33, 117, 738
BmtI	GCTAGC	1 cut(s)	1445
BpmI	CTGGAG	4 cut(s)	151, 175, 417, 1526
BpuEI	CTTGAG	1 cut(s)	452
BpuMI	CCSGG	2 cut(s)	122, 1643
Bsa29I	ATCGAT	1 cut(s)	1424
BsaBI	GATNNNNATC	1 cut(s)	1200
BsaHI	GRCGYC	2 cut(s)	198, 209
BsaI	GGTCTC	1 cut(s)	695
BsaJI	CCNNGG	3 cut(s)	620, 685, 1332
BsaWI	WCCGGW	1 cut(s)	1284
BsaXI	ACNNNNNCTCC	2 cut(s)	1684, 1714
Bsc4I	CCNNNNNNNGG	5 cut(s)	218, 1291, 1498, 1627, 1648
Bse118I	RCCGGY	3 cut(s)	136, 218, 1637
Bse1I	ACTGG	7 cut(s)	105, 192, 477, 632, 835, 1101, 1699
Bse3DI	GCAATG	2 cut(s)	577, 1386
Bse8I	GATNNNNATC	1 cut(s)	1200
BseAI	TCCGGA	1 cut(s)	1284
BseBI	CCWGG	2 cut(s)	392, 637
BseCI	ATCGAT	1 cut(s)	1424
BseDI	CCNNGG	3 cut(s)	620, 685, 1332
BseGI	GGATG	3 cut(s)	711, 753, 1518
BseJI	GATNNNNATC	1 cut(s)	1200
BseLI	CCNNNNNNNGG	5 cut(s)	218, 1291, 1498, 1627, 1648
BseMI	GCAATG	2 cut(s)	577, 1386
BseMII	CTCAG	3 cut(s)	833, 901, 956
BseNI	ACTGG	7 cut(s)	105, 192, 477, 632, 835, 1101, 1699
BseRI	GAGGAG	1 cut(s)	266
BseSI	GKGCMC	2 cut(s)	662, 1090
BseXI	GCAGC	5 cut(s)	313, 362, 1041, 1153, 1412
BsgI	GTGCAG	1 cut(s)	945
Bsh1236I	CGCG	3 cut(s)	36, 77, 159
Bsh1285I	CGRYCG	1 cut(s)	61
BshFI	GGCC	5 cut(s)	125, 140, 290, 549, 1641
BshVI	ATCGAT	1 cut(s)	1424
BsiEI	CGRYCG	1 cut(s)	61
BsiHKAI	GWGCWC	6 cut(s)	33, 117, 167, 558, 662, 1702
BsiSI	CCGG	7 cut(s)	122, 137, 178, 219, 1285, 1638, 1643
BslFI	GGGAC	1 cut(s)	450
BslI	CCNNNNNNNGG	5 cut(s)	218, 1291, 1498, 1627, 1648
BsmAI	GTCTC	2 cut(s)	71, 695
BsmFI	GGGAC	1 cut(s)	450
BsmI	GAATGC	2 cut(s)	171, 637
BsnI	GGCC	5 cut(s)	125, 140, 290, 549, 1641
Bso31I	GGTCTC	1 cut(s)	695
Bsp1286I	GDGCHC	7 cut(s)	33, 117, 167, 558, 662, 1090, 1702
Bsp13I	TCCGGA	1 cut(s)	1284
Bsp143I	GATC	8 cut(s)	58, 343, 523, 856, 880, 1354, 1459, 1501
Bsp19I	CCATGG	1 cut(s)	1332
BspACI	CCGC	8 cut(s)	22, 34, 39, 75, 89, 159, 187, 406
BspANI	GGCC	5 cut(s)	125, 140, 290, 549, 1641
BspCNI	CTCAG	3 cut(s)	832, 900, 957
BspDI	ATCGAT	1 cut(s)	1424
BspEI	TCCGGA	1 cut(s)	1284
BspFNI	CGCG	3 cut(s)	36, 77, 159
BspHI	TCATGA	1 cut(s)	1135
BspLI	GGNNCC	3 cut(s)	438, 578, 1124
BspMAI	CTGCAG	1 cut(s)	651
BspOI	GCTAGC	1 cut(s)	1445
BspPI	GGATC	2 cut(s)	518, 1509
BspQI	GCTCTTC	3 cut(s)	316, 342, 1278
BspTNI	GGTCTC	1 cut(s)	695
BsrBI	CCGCTC	1 cut(s)	189
BsrDI	GCAATG	2 cut(s)	577, 1386
BsrFI	RCCGGY	3 cut(s)	136, 218, 1637
BsrI	ACTGG	7 cut(s)	105, 192, 477, 632, 835, 1101, 1699
BssAI	RCCGGY	3 cut(s)	136, 218, 1637
BssECI	CCNNGG	3 cut(s)	620, 685, 1332
BssMI	GATC	8 cut(s)	58, 343, 523, 856, 880, 1354, 1459, 1501
BssNI	GRCGYC	2 cut(s)	198, 209
BssT1I	CCWWGG	3 cut(s)	620, 685, 1332
Bst2UI	CCWGG	2 cut(s)	392, 637
Bst4CI	ACNGT	5 cut(s)	537, 592, 667, 842, 1616
Bst6I	CTCTTC	5 cut(s)	316, 342, 1278, 1470, 1518
BstACI	GRCGYC	2 cut(s)	198, 209
BstC8I	GCNNGC	5 cut(s)	50, 138, 220, 1188, 1443
BstDEI	CTNAG	6 cut(s)	743, 819, 887, 965, 1446, 1670
BstDSI	CCRYGG	1 cut(s)	1332
BstF5I	GGATG	3 cut(s)	711, 753, 1518
BstFNI	CGCG	3 cut(s)	36, 77, 159
BstH2I	RGCGCY	1 cut(s)	1723
BstHHI	GCGC	4 cut(s)	38, 159, 375, 1722
BstKTI	GATC	8 cut(s)	61, 346, 526, 859, 883, 1357, 1462, 1504
BstMAI	GTCTC	2 cut(s)	71, 695
BstMBI	GATC	8 cut(s)	58, 343, 523, 856, 880, 1354, 1459, 1501
BstMCI	CGRYCG	1 cut(s)	61
BstMWI	GCNNNNNNNGC	3 cut(s)	332, 824, 1175
BstNI	CCWGG	2 cut(s)	392, 637
BstNSI	RCATGY	1 cut(s)	1190
BstSCI	CCNGG	4 cut(s)	120, 390, 635, 1641
BstSFI	CTRYAG	3 cut(s)	647, 663, 1191
BstSLI	GKGCMC	2 cut(s)	662, 1090
BstUI	CGCG	3 cut(s)	36, 77, 159
BstV1I	GCAGC	5 cut(s)	313, 362, 1041, 1153, 1412
BstX2I	RGATCY	2 cut(s)	880, 1501
BstYI	RGATCY	2 cut(s)	880, 1501
Bsu15I	ATCGAT	1 cut(s)	1424
BsuRI	GGCC	5 cut(s)	125, 140, 290, 549, 1641
BsuTUI	ATCGAT	1 cut(s)	1424
BtgI	CCRYGG	1 cut(s)	1332
BtsCI	GGATG	3 cut(s)	711, 753, 1518
BtsIMutI	CAGTG	2 cut(s)	199, 962
Cac8I	GCNNGC	5 cut(s)	50, 138, 220, 1188, 1443
CaiI	CAGNNNCTG	1 cut(s)	1169
CciI	TCATGA	1 cut(s)	1135
CfoI	GCGC	4 cut(s)	38, 159, 375, 1722
Cfr10I	RCCGGY	3 cut(s)	136, 218, 1637
Cfr13I	GGNCC	4 cut(s)	437, 1122, 1535, 1640
ClaI	ATCGAT	1 cut(s)	1424
CseI	GACGC	3 cut(s)	66, 217, 1184
Csp6I	GTAC	7 cut(s)	516, 538, 588, 838, 1075, 1470, 1617
CviAII	CATG	7 cut(s)	332, 785, 916, 1058, 1136, 1187, 1333
CviQI	GTAC	7 cut(s)	516, 538, 588, 838, 1075, 1470, 1617
DdeI	CTNAG	6 cut(s)	743, 819, 887, 965, 1446, 1670
DpnI	GATC	8 cut(s)	60, 345, 525, 858, 882, 1356, 1461, 1503
DpnII	GATC	8 cut(s)	58, 343, 523, 856, 880, 1354, 1459, 1501
EaeI	YGGCCR	3 cut(s)	123, 138, 288
Eam1104I	CTCTTC	5 cut(s)	316, 342, 1278, 1470, 1518
EarI	CTCTTC	5 cut(s)	316, 342, 1278, 1470, 1518
Ecl136II	GAGCTC	2 cut(s)	31, 165
Eco130I	CCWWGG	3 cut(s)	620, 685, 1332
Eco24I	GRGCYC	2 cut(s)	33, 167
Eco31I	GGTCTC	1 cut(s)	695
Eco47I	GGWCC	3 cut(s)	437, 1122, 1535
Eco47III	AGCGCT	1 cut(s)	1721
Eco53kI	GAGCTC	2 cut(s)	31, 165
Eco57I	CTGAAG	1 cut(s)	1561
EcoICRI	GAGCTC	2 cut(s)	31, 165
EcoRII	CCWGG	2 cut(s)	390, 635
EcoT14I	CCWWGG	3 cut(s)	620, 685, 1332
EcoT38I	GRGCYC	2 cut(s)	33, 167
ErhI	CCWWGG	3 cut(s)	620, 685, 1332
FaeI	CATG	7 cut(s)	335, 788, 919, 1061, 1139, 1190, 1336
FalI	AAGNNNNNCTT	4 cut(s)	284, 316, 701, 733
FaqI	GGGAC	1 cut(s)	450
FatI	CATG	7 cut(s)	331, 784, 915, 1057, 1135, 1186, 1332
FauI	CCCGC	2 cut(s)	82, 152
FbaI	TGATCA	2 cut(s)	343, 1354
FblI	GTMKAC	2 cut(s)	236, 1611
Fnu4HI	GCNGC	7 cut(s)	39, 75, 327, 376, 1055, 1167, 1401
FokI	GGATG	3 cut(s)	718, 760, 1525
FriOI	GRGCYC	2 cut(s)	33, 167
Fsp4HI	GCNGC	7 cut(s)	39, 75, 327, 376, 1055, 1167, 1401
FspBI	CTAG	3 cut(s)	443, 860, 1442
GlaI	GCGC	4 cut(s)	37, 158, 374, 1721
GluI	GCNGC	7 cut(s)	39, 75, 327, 376, 1055, 1167, 1401
GsuI	CTGGAG	4 cut(s)	151, 175, 417, 1526
HaeII	RGCGCY	1 cut(s)	1723
HaeIII	GGCC	5 cut(s)	125, 140, 290, 549, 1641
HapII	CCGG	7 cut(s)	122, 137, 178, 219, 1285, 1638, 1643
HgaI	GACGC	3 cut(s)	66, 217, 1184
HhaI	GCGC	4 cut(s)	38, 159, 375, 1722
Hin1I	GRCGYC	2 cut(s)	198, 209
Hin1II	CATG	7 cut(s)	335, 788, 919, 1061, 1139, 1190, 1336
Hin6I	GCGC	4 cut(s)	36, 157, 373, 1720
HinP1I	GCGC	4 cut(s)	36, 157, 373, 1720
HincII	GTYRAC	3 cut(s)	237, 670, 1174
HindII	GTYRAC	3 cut(s)	237, 670, 1174
HindIII	AAGCTT	1 cut(s)	1239
HinfI	GANTC	9 cut(s)	150, 183, 238, 463, 602, 758, 1013, 1313, 1421
HpaII	CCGG	7 cut(s)	122, 137, 178, 219, 1285, 1638, 1643
Hpy188I	TCNGA	4 cut(s)	243, 822, 1206, 1580
Hpy188III	TCNNGA	8 cut(s)	205, 341, 467, 676, 889, 1136, 1285, 1505
Hpy99I	CGWCG	1 cut(s)	211
HpyAV	CCTTC	6 cut(s)	293, 703, 860, 1492, 1526, 1713
HpyCH4III	ACNGT	5 cut(s)	537, 592, 667, 842, 1616
HpyCH4IV	ACGT	2 cut(s)	198, 1468
HpyCH4V	TGCA	9 cut(s)	309, 649, 660, 751, 827, 944, 962, 1023, 1057
HpyF10VI	GCNNNNNNNGC	3 cut(s)	332, 824, 1175
HpyF3I	CTNAG	6 cut(s)	743, 819, 887, 965, 1446, 1670
HpySE526I	ACGT	2 cut(s)	198, 1468
Hsp92I	GRCGYC	2 cut(s)	198, 209
Hsp92II	CATG	7 cut(s)	335, 788, 919, 1061, 1139, 1190, 1336
HspAI	GCGC	4 cut(s)	36, 157, 373, 1720
Kpn2I	TCCGGA	1 cut(s)	1284
KroI	GCCGGC	2 cut(s)	136, 218
KroNI	GCCGGC	2 cut(s)	138, 220
Ksp22I	TGATCA	2 cut(s)	343, 1354
Kzo9I	GATC	8 cut(s)	58, 343, 523, 856, 880, 1354, 1459, 1501
LguI	GCTCTTC	3 cut(s)	316, 342, 1278
LmnI	GCTCC	5 cut(s)	36, 162, 170, 194, 1705
Lsp1109I	GCAGC	5 cut(s)	313, 362, 1041, 1153, 1412
LweI	GCATC	3 cut(s)	33, 117, 738
MaeI	CTAG	3 cut(s)	443, 860, 1442
MaeII	ACGT	2 cut(s)	198, 1468
MaeIII	GTNAC	3 cut(s)	194, 968, 1388
MalI	GATC	8 cut(s)	60, 345, 525, 858, 882, 1356, 1461, 1503
MbiI	CCGCTC	1 cut(s)	189
MboI	GATC	8 cut(s)	58, 343, 523, 856, 880, 1354, 1459, 1501
MflI	RGATCY	2 cut(s)	880, 1501
MhlI	GDGCHC	7 cut(s)	33, 117, 167, 558, 662, 1090, 1702
MlsI	TGGCCA	1 cut(s)	290
MluNI	TGGCCA	1 cut(s)	290
MlyI	GAGTC	2 cut(s)	232, 611
Mox20I	TGGCCA	1 cut(s)	290
MroI	TCCGGA	1 cut(s)	1284
MroNI	GCCGGC	2 cut(s)	136, 218
MroXI	GAANNNNTTC	2 cut(s)	713, 882
MscI	TGGCCA	1 cut(s)	290
MseI	TTAA	3 cut(s)	609, 1046, 1451
MslI	CAYNNNNRTG	1 cut(s)	920
Msp20I	TGGCCA	1 cut(s)	290
MspA1I	CMGCKG	1 cut(s)	1166
MspI	CCGG	7 cut(s)	122, 137, 178, 219, 1285, 1638, 1643
MspR9I	CCNGG	4 cut(s)	122, 392, 637, 1643
Mva1269I	GAATGC	2 cut(s)	171, 637
MvaI	CCWGG	2 cut(s)	392, 637
MvnI	CGCG	3 cut(s)	36, 77, 159
MwoI	GCNNNNNNNGC	3 cut(s)	332, 824, 1175
NaeI	GCCGGC	2 cut(s)	138, 220
NciI	CCSGG	2 cut(s)	122, 1643
NcoI	CCATGG	1 cut(s)	1332
NdeII	GATC	8 cut(s)	58, 343, 523, 856, 880, 1354, 1459, 1501
NgoMIV	GCCGGC	2 cut(s)	136, 218
NheI	GCTAGC	1 cut(s)	1441
NlaIII	CATG	7 cut(s)	335, 788, 919, 1061, 1139, 1190, 1336
NlaIV	GGNNCC	3 cut(s)	438, 578, 1124
NmuCI	GTSAC	1 cut(s)	194
NspI	RCATGY	1 cut(s)	1190
PaeI	GCATGC	1 cut(s)	1190
PagI	TCATGA	1 cut(s)	1135
PciSI	GCTCTTC	3 cut(s)	316, 342, 1278
PcsI	WCGNNNNNNNCGW	1 cut(s)	233
PctI	GAATGC	2 cut(s)	171, 637
PdiI	GCCGGC	2 cut(s)	138, 220
PdmI	GAANNNNTTC	2 cut(s)	713, 882
PfeI	GAWTC	7 cut(s)	150, 183, 463, 758, 1013, 1313, 1421
PkrI	GCNGC	7 cut(s)	40, 76, 328, 377, 1056, 1168, 1402
Ple19I	CGATCG	1 cut(s)	61
PleI	GAGTC	2 cut(s)	232, 610
PpsI	GAGTC	2 cut(s)	232, 610
Psp124BI	GAGCTC	2 cut(s)	33, 167
Psp6I	CCWGG	2 cut(s)	390, 635
PspGI	CCWGG	2 cut(s)	390, 635
PspN4I	GGNNCC	3 cut(s)	438, 578, 1124
PspPI	GGNCC	4 cut(s)	437, 1122, 1535, 1640
PstI	CTGCAG	1 cut(s)	651
PstNI	CAGNNNCTG	1 cut(s)	1169
PsuI	RGATCY	2 cut(s)	880, 1501
PvuI	CGATCG	1 cut(s)	61
PvuII	CAGCTG	1 cut(s)	1166
RsaI	GTAC	7 cut(s)	517, 539, 589, 839, 1076, 1471, 1618
RsaNI	GTAC	7 cut(s)	516, 538, 588, 838, 1075, 1470, 1617
RseI	CAYNNNNRTG	1 cut(s)	920
SacI	GAGCTC	2 cut(s)	33, 167
SalI	GTCGAC	1 cut(s)	235
SapI	GCTCTTC	3 cut(s)	316, 342, 1278
SaqAI	TTAA	3 cut(s)	609, 1046, 1451
SatI	GCNGC	7 cut(s)	39, 75, 327, 376, 1055, 1167, 1401
Sau3AI	GATC	8 cut(s)	58, 343, 523, 856, 880, 1354, 1459, 1501
Sau96I	GGNCC	4 cut(s)	437, 1122, 1535, 1640
SchI	GAGTC	2 cut(s)	232, 611
ScrFI	CCNGG	4 cut(s)	122, 392, 637, 1643
SduI	GDGCHC	7 cut(s)	33, 117, 167, 558, 662, 1090, 1702
SfaNI	GCATC	3 cut(s)	33, 117, 738
SfcI	CTRYAG	3 cut(s)	647, 663, 1191
SinI	GGWCC	3 cut(s)	437, 1122, 1535
SmiMI	CAYNNNNRTG	1 cut(s)	920
SmlI	CTYRAG	1 cut(s)	431
SmoI	CTYRAG	1 cut(s)	431
SphI	GCATGC	1 cut(s)	1190
SsiI	CCGC	8 cut(s)	22, 34, 39, 75, 89, 159, 187, 406
SspMI	CTAG	3 cut(s)	443, 860, 1442
SstI	GAGCTC	2 cut(s)	33, 167
StyD4I	CCNGG	4 cut(s)	120, 390, 635, 1641
StyI	CCWWGG	3 cut(s)	620, 685, 1332
TaaI	ACNGT	5 cut(s)	537, 592, 667, 842, 1616
TaiI	ACGT	2 cut(s)	201, 1471
TaqI	TCGA	6 cut(s)	27, 111, 206, 236, 904, 1424
TaqII	GACCGA	1 cut(s)	1010
TatI	WGTACW	3 cut(s)	537, 837, 1074
TauI	GCSGC	2 cut(s)	41, 77
TfiI	GAWTC	7 cut(s)	150, 183, 463, 758, 1013, 1313, 1421
Tru1I	TTAA	3 cut(s)	609, 1046, 1451
Tru9I	TTAA	3 cut(s)	609, 1046, 1451
TscAI	CASTG	2 cut(s)	199, 969
TseFI	GTSAC	1 cut(s)	194
TseI	GCWGC	5 cut(s)	326, 375, 1054, 1166, 1400
Tsp45I	GTSAC	1 cut(s)	194
TspDTI	ATGAA	9 cut(s)	17, 722, 945, 1152, 1166, 1265, 1389, 1397, 1536
TspRI	CASTG	2 cut(s)	199, 969
VneI	GTGCAC	1 cut(s)	658
VpaK11BI	GGWCC	3 cut(s)	437, 1122, 1535
XapI	RAATTY	3 cut(s)	851, 870, 1139
XceI	RCATGY	1 cut(s)	1190
XmiI	GTMKAC	2 cut(s)	236, 1611
XmnI	GAANNNNTTC	2 cut(s)	713, 882
XspI	CTAG	3 cut(s)	443, 860, 1442
ZraI	GACGTC	1 cut(s)	199

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.