AT1G65040

E3 ubiquitin-protein ligase synoviolin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003784
Physical Location & Seq
Reverse (-)
24159685 .. 24163669
3985 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65040.5

Sequence Viewer

Length: 846 bp
ATGATTCTGGCAACAACAACTGTTTCTATTATTGTGAAGTATGCCTTCTATGTCACTGATATGCTCAAGGAAGGTCAATGGGAAGGAAAACCAGTATACACTTTCTATCTGGAGCTTGTTCGTGACTTGCTTCACTTGTCCATGTATCTTTGCTTCTTCCTGATGATATTCATGAACTATGGCCTTCCCTTGCATCTAATACGGGAGCTCTACGAAACGTTCAGAAACTTCAAGATTCGTGTGACTGATTACCTTCGATATCGGAAAATCACTTCCAATATGAATGATCGGTTTCCTGATGCAACTCCTGAAGAACTTAGTTCAAATGATGCCACTTGTATCATATGTCGTGAAGAAATGACCTCAGCGAAGAAATTAGTATGTGGCCATCTATTTCATGTACATTGTCTTCGGTCATGGTTGGAACGACAGAACACATGTCCCACTTGCAGAGCACTGGTTGTCCCAGCTGAGAATGCTACATCAACAGCTTCTGGAAACCGTGGGCCACACCAAGAATCCCTGCAGCAAGGAACCGGTACTTCAAGTTCTGATGGTCAGGGTTCTTCTGTTTCTGCTGCTGCCAGTGAGAATATGAGCAGGCACGAGGCTAGGTTTCAAGCCGCTGCTTCTGCAGCCTCTATATATGGGAGATCCATTGTTTATCCATCTTCTGCAAACACACTAGTATGGTCTCAAGGTAATTCATTACTTCCTCAGACGGAGGTAGAAGCTCAAAGAAGATTTCTTGAGTCCCAAATCGAGGTGTTAACGAACCAACTTCGGCTTTTGGAGAAACCGACAACTGTAGATACGAAAGGAAAGTCCGTTGCGGATACTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000153 GO:0000209 GO:0000835 GO:0000836 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0023052 GO:0030163 GO:0030433 GO:0030968 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034620 GO:0034645 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070085 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071944 GO:0098796 GO:0098827 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1902494 GO:1990234 GO:1990381
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 map04120 map04141
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

281

Amino Acids

31.59

Weight (kDa)

7.04

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_SYVN1_N PF25563 25 - 119 1.2e-34 E3 ubiquitin-protein ligase synoviolin-like, TPR repeats
zf-rbx1 PF12678 136 - 177 2.6e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 138 - 177 2.4e-12 Ring finger domain
zf-C3HC4 PF00097 139 - 176 3.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 139 - 176 4.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 139 - 174 6.4e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 96
AciI CCGC 2 cut(s) 624, 833
AclI AACGTT 1 cut(s) 218
AclWI GGATC 1 cut(s) 648
AcoI YGGCCR 1 cut(s) 385
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 2 cut(s) 402, 541
AfiI CCNNNNNNNGG 1 cut(s) 763
AflIII ACRYGT 1 cut(s) 437
AgeI ACCGGT 1 cut(s) 536
AgsI TTSAA 4 cut(s) 232, 324, 546, 620
AhlI ACTAGT 1 cut(s) 685
AluBI AGCT 5 cut(s) 115, 208, 470, 491, 734
AluI AGCT 5 cut(s) 115, 208, 470, 491, 734
Alw21I GWGCWC 2 cut(s) 210, 457
Alw26I GTCTC 1 cut(s) 699
AlwI GGATC 1 cut(s) 648
AlwNI CAGNNNCTG 1 cut(s) 494
AoxI GGCC 3 cut(s) 181, 385, 506
ApeKI GCWGC 5 cut(s) 526, 578, 581, 626, 635
ArsI GACNNNNNNTTYG 2 cut(s) 809, 841
AsiGI ACCGGT 1 cut(s) 536
AspS9I GGNCC 1 cut(s) 506
BalI TGGCCA 1 cut(s) 387
BanII GRGCYC 1 cut(s) 210
BarI GAAGNNNNNNTAC 4 cut(s) 137, 169, 393, 425
BauI CACGAG 1 cut(s) 605
BbsI GAAGAC 1 cut(s) 401
Bbv12I GWGCWC 2 cut(s) 210, 457
BbvCI CCTCAGC 1 cut(s) 364
BbvI GCAGC 5 cut(s) 538, 565, 568, 613, 647
BccI CCATC 3 cut(s) 396, 548, 676
BciVI GTATCC 1 cut(s) 829
BcoDI GTCTC 1 cut(s) 699
BcuI ACTAGT 1 cut(s) 685
BfaI CTAG 2 cut(s) 612, 686
BfmI CTRYAG 3 cut(s) 524, 633, 807
BfuI GTATCC 1 cut(s) 829
BisI GCNGC 6 cut(s) 527, 579, 582, 624, 627, 636
BlsI GCNGC 6 cut(s) 528, 580, 583, 625, 628, 637
BmgT120I GGNCC 1 cut(s) 506
BmiI GGNNCC 1 cut(s) 535
BmsI GCATC 3 cut(s) 202, 289, 319
BpiI GAAGAC 1 cut(s) 401
BpmI CTGGAG 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 364
BpuEI CTTGAG 3 cut(s) 50, 681, 770
BsaI GGTCTC 1 cut(s) 699
BsaJI CCNNGG 1 cut(s) 502
BsaWI WCCGGW 1 cut(s) 536
Bsc4I CCNNNNNNNGG 1 cut(s) 763
Bse118I RCCGGY 1 cut(s) 536
Bse1I ACTGG 3 cut(s) 92, 462, 585
BseDI CCNNGG 1 cut(s) 502
BseLI CCNNNNNNNGG 1 cut(s) 763
BseMII CTCAG 3 cut(s) 378, 462, 731
BseNI ACTGG 3 cut(s) 92, 462, 585
BseXI GCAGC 5 cut(s) 538, 565, 568, 613, 647
BseYI CCCAGC 1 cut(s) 466
BshFI GGCC 3 cut(s) 183, 387, 508
BshTI ACCGGT 1 cut(s) 536
BsiHKAI GWGCWC 2 cut(s) 210, 457
BsiSI CCGG 1 cut(s) 537
BslFI GGGAC 3 cut(s) 426, 449, 739
BslI CCNNNNNNNGG 1 cut(s) 763
BsmAI GTCTC 1 cut(s) 699
BsmFI GGGAC 3 cut(s) 426, 449, 739
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 3 cut(s) 183, 387, 508
Bso31I GGTCTC 1 cut(s) 699
Bsp1286I GDGCHC 2 cut(s) 210, 457
Bsp1407I TGTACA 1 cut(s) 400
Bsp143I GATC 2 cut(s) 286, 653
BspACI CCGC 2 cut(s) 624, 833
BspANI GGCC 3 cut(s) 183, 387, 508
BspCNI CTCAG 3 cut(s) 377, 463, 730
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 1 cut(s) 535
BspMAI CTGCAG 2 cut(s) 528, 637
BspPI GGATC 1 cut(s) 648
BspTNI GGTCTC 1 cut(s) 699
BsrFI RCCGGY 1 cut(s) 536
BsrGI TGTACA 1 cut(s) 400
BsrI ACTGG 3 cut(s) 92, 462, 585
BssAI RCCGGY 1 cut(s) 536
BssECI CCNNGG 1 cut(s) 502
BssMI GATC 2 cut(s) 286, 653
BssNAI GTATAC 1 cut(s) 97
BssSI CACGAG 1 cut(s) 605
Bst1107I GTATAC 1 cut(s) 97
Bst2BI CACGAG 1 cut(s) 605
Bst4CI ACNGT 3 cut(s) 22, 503, 808
BstAUI TGTACA 1 cut(s) 400
BstC8I GCNNGC 1 cut(s) 602
BstDEI CTNAG 4 cut(s) 317, 364, 471, 717
BstDSI CCRYGG 1 cut(s) 502
BstKTI GATC 2 cut(s) 289, 656
BstMAI GTCTC 1 cut(s) 699
BstMBI GATC 2 cut(s) 286, 653
BstMWI GCNNNNNNNGC 4 cut(s) 476, 632, 635, 839
BstNSI RCATGY 1 cut(s) 441
BstSFI CTRYAG 3 cut(s) 524, 633, 807
BstV1I GCAGC 5 cut(s) 538, 565, 568, 613, 647
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 1 cut(s) 653
BstYI RGATCY 1 cut(s) 653
BstZ17I GTATAC 1 cut(s) 97
BsuI GTATCC 1 cut(s) 829
BsuRI GGCC 3 cut(s) 183, 387, 508
BtgI CCRYGG 1 cut(s) 502
BtsIMutI CAGTG 3 cut(s) 54, 455, 592
Cac8I GCNNGC 1 cut(s) 602
CaiI CAGNNNCTG 1 cut(s) 494
CciI TCATGA 1 cut(s) 171
Cfr10I RCCGGY 1 cut(s) 536
Cfr13I GGNCC 1 cut(s) 506
Csp6I GTAC 2 cut(s) 401, 540
CspAI ACCGGT 1 cut(s) 536
CviAII CATG 5 cut(s) 142, 172, 398, 417, 438
CviQI GTAC 2 cut(s) 401, 540
DdeI CTNAG 4 cut(s) 317, 364, 471, 717
DpnI GATC 2 cut(s) 288, 655
DpnII GATC 2 cut(s) 286, 653
EaeI YGGCCR 1 cut(s) 385
Ecl136II GAGCTC 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 210
Eco31I GGTCTC 1 cut(s) 699
Eco32I GATATC 1 cut(s) 260
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 208
EcoRV GATATC 1 cut(s) 260
EcoT38I GRGCYC 1 cut(s) 210
FaeI CATG 5 cut(s) 145, 175, 401, 420, 441
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 3 cut(s) 426, 449, 739
FatI CATG 5 cut(s) 141, 171, 397, 416, 437
FauNDI CATATG 1 cut(s) 344
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 6 cut(s) 527, 579, 582, 624, 627, 636
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 6 cut(s) 527, 579, 582, 624, 627, 636
FspBI CTAG 2 cut(s) 612, 686
GluI GCNGC 6 cut(s) 527, 579, 582, 624, 627, 636
GsaI CCCAGC 1 cut(s) 470
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 3 cut(s) 183, 387, 508
HapII CCGG 1 cut(s) 537
Hin1II CATG 5 cut(s) 145, 175, 401, 420, 441
HincII GTYRAC 1 cut(s) 771
HindII GTYRAC 1 cut(s) 771
HinfI GANTC 4 cut(s) 4, 235, 518, 752
HpaI GTTAAC 1 cut(s) 771
HpaII CCGG 1 cut(s) 537
Hpy166II GTNNAC 2 cut(s) 97, 771
Hpy188I TCNGA 4 cut(s) 224, 264, 553, 720
Hpy8I GTNNAC 2 cut(s) 97, 771
HpyAV CCTTC 5 cut(s) 55, 65, 77, 194, 263
HpyCH4III ACNGT 3 cut(s) 22, 503, 808
HpyCH4IV ACGT 1 cut(s) 218
HpyCH4V TGCA 7 cut(s) 193, 302, 450, 526, 635, 677, 842
HpyF10VI GCNNNNNNNGC 4 cut(s) 476, 632, 635, 839
HpyF3I CTNAG 4 cut(s) 317, 364, 471, 717
HpySE526I ACGT 1 cut(s) 218
Hsp92II CATG 5 cut(s) 145, 175, 401, 420, 441
KspAI GTTAAC 1 cut(s) 771
Kzo9I GATC 2 cut(s) 286, 653
LmnI GCTCC 2 cut(s) 112, 205
Lsp1109I GCAGC 5 cut(s) 538, 565, 568, 613, 647
LweI GCATC 3 cut(s) 202, 289, 319
MaeI CTAG 2 cut(s) 612, 686
MaeII ACGT 1 cut(s) 218
MaeIII GTNAC 3 cut(s) 52, 122, 241
MalI GATC 2 cut(s) 288, 655
MboI GATC 2 cut(s) 286, 653
MboII GAAGA 8 cut(s) 148, 323, 365, 382, 401, 558, 663, 753
MflI RGATCY 1 cut(s) 653
MhlI GDGCHC 2 cut(s) 210, 457
MlsI TGGCCA 1 cut(s) 387
MluCI AATT 2 cut(s) 374, 703
MluNI TGGCCA 1 cut(s) 387
MlyI GAGTC 1 cut(s) 761
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 6 cut(s) 373, 601, 649, 718, 726, 757
Mox20I TGGCCA 1 cut(s) 387
MscI TGGCCA 1 cut(s) 387
MseI TTAA 1 cut(s) 770
MslI CAYNNNNRTG 2 cut(s) 59, 688
Msp20I TGGCCA 1 cut(s) 387
MspA1I CMGCKG 2 cut(s) 470, 626
MspI CCGG 1 cut(s) 537
Mva1269I GAATGC 1 cut(s) 481
MwoI GCNNNNNNNGC 4 cut(s) 476, 632, 635, 839
NdeI CATATG 1 cut(s) 344
NdeII GATC 2 cut(s) 286, 653
NlaIII CATG 5 cut(s) 145, 175, 401, 420, 441
NlaIV GGNNCC 1 cut(s) 535
NmuCI GTSAC 3 cut(s) 52, 122, 241
NspI RCATGY 1 cut(s) 441
PagI TCATGA 1 cut(s) 171
PciI ACATGT 1 cut(s) 437
PctI GAATGC 1 cut(s) 481
PfeI GAWTC 3 cut(s) 4, 235, 518
PinAI ACCGGT 1 cut(s) 536
PkrI GCNGC 6 cut(s) 528, 580, 583, 625, 628, 637
PleI GAGTC 1 cut(s) 760
PpsI GAGTC 1 cut(s) 760
PscI ACATGT 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 210
Psp1406I AACGTT 1 cut(s) 218
PspFI CCCAGC 1 cut(s) 466
PspN4I GGNNCC 1 cut(s) 535
PspPI GGNCC 1 cut(s) 506
PsrI GAACNNNNNNTAC 2 cut(s) 532, 564
PstI CTGCAG 2 cut(s) 528, 637
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 653
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 2 cut(s) 402, 541
RsaNI GTAC 2 cut(s) 401, 540
RseI CAYNNNNRTG 2 cut(s) 59, 688
SacI GAGCTC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 770
SatI GCNGC 6 cut(s) 527, 579, 582, 624, 627, 636
Sau3AI GATC 2 cut(s) 286, 653
Sau96I GGNCC 1 cut(s) 506
SchI GAGTC 1 cut(s) 761
SduI GDGCHC 2 cut(s) 210, 457
SfaNI GCATC 3 cut(s) 202, 289, 319
SfcI CTRYAG 3 cut(s) 524, 633, 807
SmiMI CAYNNNNRTG 2 cut(s) 59, 688
SmlI CTYRAG 3 cut(s) 65, 696, 749
SmoI CTYRAG 3 cut(s) 65, 696, 749
SpeI ACTAGT 1 cut(s) 685
Sse9I AATT 2 cut(s) 374, 703
SsiI CCGC 2 cut(s) 624, 833
SspMI CTAG 2 cut(s) 612, 686
SstI GAGCTC 1 cut(s) 210
TaaI ACNGT 3 cut(s) 22, 503, 808
TaiI ACGT 1 cut(s) 221
TaqI TCGA 2 cut(s) 256, 762
TaqII GACCGA 1 cut(s) 402
TasI AATT 2 cut(s) 374, 703
TatI WGTACW 1 cut(s) 400
TauI GCSGC 1 cut(s) 626
TfiI GAWTC 3 cut(s) 4, 235, 518
Tru1I TTAA 1 cut(s) 770
Tru9I TTAA 1 cut(s) 770
TscAI CASTG 3 cut(s) 61, 462, 592
TseFI GTSAC 3 cut(s) 52, 122, 241
TseI GCWGC 5 cut(s) 526, 578, 581, 626, 635
Tsp45I GTSAC 3 cut(s) 52, 122, 241
TspDTI ATGAA 5 cut(s) 160, 188, 296, 386, 696
TspGWI ACGGA 2 cut(s) 737, 817
TspRI CASTG 3 cut(s) 61, 462, 592
XceI RCATGY 1 cut(s) 441
XmiI GTMKAC 1 cut(s) 96
XspI CTAG 2 cut(s) 612, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.