pycom01g24310

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003784
Physical Location & Seq
Reverse (-)
21284365 .. 21286054
1690 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g24310.1

Sequence Viewer

Length: 1080 bp
ATGTGGAGATTTTTGCTTGTTGACGTGCATAAGCTGAGGTACTATAGTGTGCCACTGCACTTGATACGTGAGGTCTATGAAACATTTAGGAACTTGAATATTCGCATTGCTGATTACATTCGTTATCGAGAGCTCACATCAAACATGAATGATCGATTCCCAGATGCTACGCATGCAGAGCTTAATGCAAGTGACGCAACTTGTATCATTTGCCGTGAAGAGATGACAACAGCCAAGAAACTATTGTGTGGACACCTTTTTCATGTGCCTTGCCTTCGATCATGGTTAGAGCAACAGCATAACTGCCCTACTTGCAGGGCCCTAGTTGTACGTCCTGACAGTGGGAAAGGTACAGCTGGCGGACATAGGGGTTCACGGTCAGATGCTCATCAACAGGGGACAGGCACAACGGCTCAGGATAGTGGAGTTGCTGGTGATGGTGTGAGCCTATATGGAGCCAGGCTCCGAGCTGCTGCTGCTGCTGCTGCATCGATTATTAGAAGTCTCATGTATATCCTTCTGCAAACAGACAATTATGGTAATAACACCTTTATCGTAAGTTGTGTGCTTGATACATATGATTTGGATATGTTGCACTGCATAGAATGCTACATACTACACCCTTACAAACTACGCCCTCTTCTGTGCGGGTCTCCTGGATATGCAGCACTTCCTCAGGTTCAAAGACATATGGTTGACTCCTCCAAGACTGAATCTAGTGGAGAAGCTTGTAGTGGACAATCACAACCGCAGCTTGCAACCCCTGGTGGACCTGCAAAGTTGTCTTTCTCTCAGTTTCCACCTTACGTGTTTGTCCCTGGAGCCAACGTGGCCTATGGGGAGAGGTCGGGCAGCGATCCAAATCTGACATCCTCTCAGCTGGAGGCCCAGAAAAAGTTTCTTCAGCACCAGATAGAGCTGATCCTGCAGAATCAGCTCCAACTTTTACAGAAACCAAAACCAAAGGAAAGTGTGCACATGGGTCCAACAAGGGCTTCTTTGGATGGCAAAGGAAAGAGTGTTGCAGCTTCTTCATCTGCTTCAGATTGTGGACGTCATGAAGATGGGGTTGATTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000153 GO:0000209 GO:0000835 GO:0000836 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0023052 GO:0030163 GO:0030433 GO:0030968 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034620 GO:0034645 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070085 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071944 GO:0098796 GO:0098827 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1902494 GO:1990234 GO:1990381
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 map04120 map04141
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

360

Amino Acids

39.41

Weight (kDa)

7.96

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_SYVN1_N PF25563 12 - 48 1.4e-08 E3 ubiquitin-protein ligase synoviolin-like, TPR repeats
zf-rbx1 PF12678 63 - 106 1.4e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 67 - 106 1.3e-10 Ring finger domain
zf-C3HC4 PF00097 68 - 105 4.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 68 - 105 8.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 68 - 103 8.4e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1057
Acc36I ACCTGC 1 cut(s) 781
AciI CCGC 3 cut(s) 360, 648, 749
AclWI GGATC 2 cut(s) 851, 916
AcuI CTGAAG 2 cut(s) 887, 1026
AcyI GRCGYC 1 cut(s) 1054
AfaI GTAC 3 cut(s) 41, 330, 352
AfiI CCNNNNNNNGG 1 cut(s) 341
AflIII ACRYGT 1 cut(s) 807
AgsI TTSAA 2 cut(s) 97, 683
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 4 cut(s) 458, 655, 763, 817
AjuI GAANNNNNNNTTGG 2 cut(s) 979, 1011
Alw21I GWGCWC 2 cut(s) 135, 978
Alw26I GTCTC 2 cut(s) 509, 657
Alw44I GTGCAC 1 cut(s) 974
AlwI GGATC 2 cut(s) 851, 916
AoxI GGCC 3 cut(s) 318, 831, 885
ApaI GGGCCC 1 cut(s) 322
ApaLI GTGCAC 1 cut(s) 974
AspS9I GGNCC 5 cut(s) 318, 319, 770, 886, 983
AsuHPI GGTGA 1 cut(s) 446
AvaII GGWCC 2 cut(s) 770, 983
AxyI CCTNAGG 1 cut(s) 675
BaeGI GKGCMC 2 cut(s) 322, 978
BaeI ACNNNNGTAYC 2 cut(s) 31, 64
BanII GRGCYC 2 cut(s) 135, 322
Bbv12I GWGCWC 2 cut(s) 135, 978
BbvCI CCTCAGC 1 cut(s) 35
BccI CCATC 3 cut(s) 431, 998, 1058
BceAI ACGGC 2 cut(s) 198, 426
BciT130I CCWGG 4 cut(s) 460, 657, 765, 819
BcoDI GTCTC 2 cut(s) 509, 657
BfaI CTAG 2 cut(s) 323, 717
BfmI CTRYAG 2 cut(s) 43, 926
BfuAI ACCTGC 1 cut(s) 781
BglI GCCNNNNNGGC 1 cut(s) 830
Bme1390I CCNGG 4 cut(s) 460, 657, 765, 819
Bme18I GGWCC 2 cut(s) 770, 983
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 5 cut(s) 318, 319, 770, 886, 983
BmiI GGNNCC 5 cut(s) 320, 457, 464, 823, 984
BmrFI CCNGG 4 cut(s) 460, 657, 765, 819
BmsI GCATC 3 cut(s) 154, 373, 497
BplI GAGNNNNNCTC 2 cut(s) 447, 479
BpmI CTGGAG 2 cut(s) 840, 902
Bpu10I CCTNAGC 2 cut(s) 35, 414
Bsa29I ATCGAT 2 cut(s) 154, 491
BsaAI YACGTR 2 cut(s) 68, 808
BsaBI GATNNNNATC 2 cut(s) 387, 861
BsaHI GRCGYC 1 cut(s) 1054
BsaI GGTCTC 1 cut(s) 657
BsaJI CCNNGG 2 cut(s) 763, 817
BsaXI ACNNNNNCTCC 2 cut(s) 813, 843
Bsc4I CCNNNNNNNGG 1 cut(s) 341
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 1 cut(s) 105
Bse8I GATNNNNATC 2 cut(s) 387, 861
BseBI CCWGG 4 cut(s) 460, 657, 765, 819
BseCI ATCGAT 2 cut(s) 154, 491
BseDI CCNNGG 2 cut(s) 763, 817
BseGI GGATG 2 cut(s) 869, 1009
BseJI GATNNNNATC 2 cut(s) 387, 861
BseLI CCNNNNNNNGG 1 cut(s) 341
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 5 cut(s) 26, 428, 689, 806, 890
BseRI GAGGAG 1 cut(s) 691
BseSI GKGCMC 2 cut(s) 322, 978
BsgI GTGCAG 1 cut(s) 41
BshFI GGCC 3 cut(s) 320, 833, 887
BshVI ATCGAT 2 cut(s) 154, 491
BsiHKAI GWGCWC 2 cut(s) 135, 978
BslFI GGGAC 2 cut(s) 412, 800
BslI CCNNNNNNNGG 1 cut(s) 341
BsmAI GTCTC 2 cut(s) 509, 657
BsmFI GGGAC 2 cut(s) 412, 800
BsmI GAATGC 1 cut(s) 611
BsnI GGCC 3 cut(s) 320, 833, 887
Bso31I GGTCTC 1 cut(s) 657
Bsp120I GGGCCC 1 cut(s) 318
Bsp1286I GDGCHC 3 cut(s) 135, 322, 978
Bsp143I GATC 4 cut(s) 151, 278, 856, 921
BspACI CCGC 3 cut(s) 360, 648, 749
BspANI GGCC 3 cut(s) 320, 833, 887
BspCNI CTCAG 5 cut(s) 27, 427, 688, 805, 889
BspDI ATCGAT 2 cut(s) 154, 491
BspHI TCATGA 1 cut(s) 1057
BspLI GGNNCC 5 cut(s) 320, 457, 464, 823, 984
BspMAI CTGCAG 1 cut(s) 930
BspMI ACCTGC 1 cut(s) 781
BspPI GGATC 2 cut(s) 851, 916
BspTNI GGTCTC 1 cut(s) 657
BsrDI GCAATG 1 cut(s) 105
BssECI CCNNGG 2 cut(s) 763, 817
BssMI GATC 4 cut(s) 151, 278, 856, 921
BssNI GRCGYC 1 cut(s) 1054
Bst2UI CCWGG 4 cut(s) 460, 657, 765, 819
Bst4CI ACNGT 2 cut(s) 341, 378
Bst6I CTCTTC 2 cut(s) 213, 645
BstACI GRCGYC 1 cut(s) 1054
BstAPI GCANNNNNTGC 1 cut(s) 606
BstBAI YACGTR 2 cut(s) 68, 808
BstC8I GCNNGC 3 cut(s) 174, 358, 756
BstDEI CTNAG 5 cut(s) 35, 414, 675, 792, 876
BstF5I GGATG 2 cut(s) 869, 1009
BstKTI GATC 4 cut(s) 154, 281, 859, 924
BstMAI GTCTC 2 cut(s) 509, 657
BstMBI GATC 4 cut(s) 151, 278, 856, 921
BstNI CCWGG 4 cut(s) 460, 657, 765, 819
BstNSI RCATGY 1 cut(s) 176
BstSCI CCNGG 4 cut(s) 458, 655, 763, 817
BstSFI CTRYAG 2 cut(s) 43, 926
BstSLI GKGCMC 2 cut(s) 322, 978
Bsu15I ATCGAT 2 cut(s) 154, 491
Bsu36I CCTNAGG 1 cut(s) 675
BsuRI GGCC 3 cut(s) 320, 833, 887
BsuTUI ATCGAT 2 cut(s) 154, 491
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 2 cut(s) 869, 1009
BtsI GCAGTG 2 cut(s) 53, 595
BtsIMutI CAGTG 3 cut(s) 53, 346, 595
BveI ACCTGC 1 cut(s) 781
Cac8I GCNNGC 3 cut(s) 174, 358, 756
CciI TCATGA 1 cut(s) 1057
Cfr13I GGNCC 5 cut(s) 318, 319, 770, 886, 983
ClaI ATCGAT 2 cut(s) 154, 491
CseI GACGC 1 cut(s) 203
Csp6I GTAC 3 cut(s) 40, 329, 351
CspCI CAANNNNNGTGG 2 cut(s) 42, 77
CviAII CATG 7 cut(s) 145, 173, 263, 282, 508, 979, 1058
CviQI GTAC 3 cut(s) 40, 329, 351
DdeI CTNAG 5 cut(s) 35, 414, 675, 792, 876
DpnI GATC 4 cut(s) 153, 280, 858, 923
DpnII GATC 4 cut(s) 151, 278, 856, 921
Eam1104I CTCTTC 2 cut(s) 213, 645
EarI CTCTTC 2 cut(s) 213, 645
EciI GGCGGA 1 cut(s) 375
Ecl136II GAGCTC 1 cut(s) 133
Eco24I GRGCYC 2 cut(s) 135, 322
Eco31I GGTCTC 1 cut(s) 657
Eco47I GGWCC 2 cut(s) 770, 983
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 887, 1026
Eco81I CCTNAGG 1 cut(s) 675
EcoICRI GAGCTC 1 cut(s) 133
EcoO109I RGGNCCY 2 cut(s) 318, 319
EcoRII CCWGG 4 cut(s) 458, 655, 763, 817
EcoT38I GRGCYC 2 cut(s) 135, 322
FaeI CATG 7 cut(s) 148, 176, 266, 285, 511, 982, 1061
FalI AAGNNNNNCTT 2 cut(s) 982, 1014
FaqI GGGAC 2 cut(s) 412, 800
FatI CATG 7 cut(s) 144, 172, 262, 281, 507, 978, 1057
FauI CCCGC 1 cut(s) 641
FauNDI CATATG 2 cut(s) 577, 690
FokI GGATG 2 cut(s) 856, 1016
FriOI GRGCYC 2 cut(s) 135, 322
FspBI CTAG 2 cut(s) 323, 717
GsuI CTGGAG 2 cut(s) 840, 902
HaeIII GGCC 3 cut(s) 320, 833, 887
HgaI GACGC 1 cut(s) 203
Hin1I GRCGYC 1 cut(s) 1054
Hin1II CATG 7 cut(s) 148, 176, 266, 285, 511, 982, 1061
HincII GTYRAC 2 cut(s) 22, 697
HindII GTYRAC 2 cut(s) 22, 697
HindIII AAGCTT 1 cut(s) 726
HinfI GANTC 5 cut(s) 156, 698, 713, 931, 1073
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 8 cut(s) 22, 251, 374, 697, 737, 770, 976, 1052
Hpy188I TCNGA 4 cut(s) 382, 467, 867, 1045
Hpy188III TCNNGA 4 cut(s) 128, 335, 416, 1058
Hpy8I GTNNAC 8 cut(s) 22, 251, 374, 697, 737, 770, 976, 1052
HpyAV CCTTC 2 cut(s) 284, 527
HpyCH4III ACNGT 2 cut(s) 341, 378
HpyCH4IV ACGT 6 cut(s) 24, 67, 331, 807, 828, 1054
HpyF3I CTNAG 5 cut(s) 35, 414, 675, 792, 876
HpySE526I ACGT 6 cut(s) 24, 67, 331, 807, 828, 1054
Hsp92I GRCGYC 1 cut(s) 1054
Hsp92II CATG 7 cut(s) 148, 176, 266, 285, 511, 982, 1061
Kzo9I GATC 4 cut(s) 151, 278, 856, 921
LmnI GCTCC 4 cut(s) 455, 468, 821, 942
LweI GCATC 3 cut(s) 154, 373, 497
MaeI CTAG 2 cut(s) 323, 717
MaeII ACGT 6 cut(s) 24, 67, 331, 807, 828, 1054
MaeIII GTNAC 1 cut(s) 191
MalI GATC 4 cut(s) 153, 280, 858, 923
MboI GATC 4 cut(s) 151, 278, 856, 921
MboII GAAGA 5 cut(s) 230, 632, 893, 1023, 1073
MhlI GDGCHC 3 cut(s) 135, 322, 978
MluCI AATT 1 cut(s) 532
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 2 cut(s) 964, 1010
MnlI CCTC 8 cut(s) 30, 64, 648, 684, 712, 837, 877, 883
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 1 cut(s) 1062
MspA1I CMGCKG 2 cut(s) 356, 880
MspR9I CCNGG 4 cut(s) 460, 657, 765, 819
Mva1269I GAATGC 1 cut(s) 611
MvaI CCWGG 4 cut(s) 460, 657, 765, 819
NdeI CATATG 2 cut(s) 577, 690
NdeII GATC 4 cut(s) 151, 278, 856, 921
NlaIII CATG 7 cut(s) 148, 176, 266, 285, 511, 982, 1061
NlaIV GGNNCC 5 cut(s) 320, 457, 464, 823, 984
NmuCI GTSAC 1 cut(s) 191
NspI RCATGY 1 cut(s) 176
PaeI GCATGC 1 cut(s) 176
PagI TCATGA 1 cut(s) 1057
PctI GAATGC 1 cut(s) 611
PfeI GAWTC 4 cut(s) 156, 713, 931, 1073
PfoI TCCNGGA 1 cut(s) 655
PleI GAGTC 1 cut(s) 692
PpsI GAGTC 1 cut(s) 692
Ppu21I YACGTR 2 cut(s) 68, 808
Psp124BI GAGCTC 1 cut(s) 135
Psp6I CCWGG 4 cut(s) 458, 655, 763, 817
PspGI CCWGG 4 cut(s) 458, 655, 763, 817
PspN4I GGNNCC 5 cut(s) 320, 457, 464, 823, 984
PspOMI GGGCCC 1 cut(s) 318
PspPI GGNCC 5 cut(s) 318, 319, 770, 886, 983
PstI CTGCAG 1 cut(s) 930
PvuII CAGCTG 2 cut(s) 356, 880
RsaI GTAC 3 cut(s) 41, 330, 352
RsaNI GTAC 3 cut(s) 40, 329, 351
RseI CAYNNNNRTG 1 cut(s) 1062
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 183
Sau3AI GATC 4 cut(s) 151, 278, 856, 921
Sau96I GGNCC 5 cut(s) 318, 319, 770, 886, 983
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 4 cut(s) 460, 657, 765, 819
SduI GDGCHC 3 cut(s) 135, 322, 978
SfaNI GCATC 3 cut(s) 154, 373, 497
SfcI CTRYAG 2 cut(s) 43, 926
SinI GGWCC 2 cut(s) 770, 983
SmiMI CAYNNNNRTG 1 cut(s) 1062
SphI GCATGC 1 cut(s) 176
Sse9I AATT 1 cut(s) 532
SsiI CCGC 3 cut(s) 360, 648, 749
SspI AATATT 1 cut(s) 100
SspMI CTAG 2 cut(s) 323, 717
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 4 cut(s) 458, 655, 763, 817
TaaI ACNGT 2 cut(s) 341, 378
TaiI ACGT 6 cut(s) 27, 70, 334, 810, 831, 1057
TaqI TCGA 4 cut(s) 127, 154, 277, 491
TasI AATT 1 cut(s) 532
TfiI GAWTC 4 cut(s) 156, 713, 931, 1073
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 3 cut(s) 60, 346, 602
TseFI GTSAC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 5 cut(s) 93, 161, 251, 1023, 1074
TspRI CASTG 3 cut(s) 60, 346, 602
VneI GTGCAC 1 cut(s) 974
VpaK11BI GGWCC 2 cut(s) 770, 983
XceI RCATGY 1 cut(s) 176
XspI CTAG 2 cut(s) 323, 717
ZraI GACGTC 1 cut(s) 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.