MD01G1235300.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003784
Physical Location & Seq
Reverse (-)
32288568 .. 32292191
3624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1235300.v1.1.491

Sequence Viewer

Length: 1635 bp
ATGATGAAGCTGCAAACCTATGCGGGGATTAGTTTGGTAGCAACCCTAGCGATTGTTTATCATGCATTTAGTAGTAGAGGTCAGTTTTATCCGGCAATGGTGTATTTGTCGACGTCGAAGATCAGCTTGGTGTTGCTTCTCAACATGGGTCTGGTCATTATGTGTACTATGTGGCAATTGACTAAAAAGATCTTCCTTGGATCACTCCGGGAAGCCGAGGTGGAGAGGCTTAACGAGCAATCGTGGCGGGAAGTCATGGAAATCCTCTTTGCCATCACTATATTCAGGCAGGACTTTTCGGTCTCGTTTCTTGCTATGGTTACTACTCTCTTGTTGATCAAGGCTTTGCATTGGTTGGCTCAGAAGAGGGTTGAGTACATCGAGACTACTCCTTCCGTGCCCATGCTGTCTCATGTTCGGATTGTCTCCTTTTTGGGTTTCTTGCTTGTTCTAGATAGTCTCTTTTTGTACAGTTCTGTTAGCAACTTGATAAGGACACAAAAGGCCTCGGTTTCCCTCTTCTTTTCATTCGAGTACATGATACTAGCAACAACAACAGTGTCAACGTTTGTGAAATATGTTTTCTATGTAACTGACATGCTTAAGGAGGGACAGTGGGAGAAGAAGCCTGTCTACACGTTCTACTTGGAACTTATTCGGGACTTGCTTCATTTGTCTATGTATTTGTGCTTCTTCCTCGCGATTTTCACGTACTATGGTGTGCCACTGCACTTGATACGTGAGCTTTATGAAACATTTAGGAACTTTAAGCTTCGCATTGCTGATTACATTCGTTATCGAAAGCTCACATCAAACATGAATGATCGATTCCCAGATGCTACGCCTGAAGAGCTTAATGCAAGTGACGCAACTTGTATCATTTGCCGTGAAGAGATGACAACAGCCAAGAAACTATTATGTGGACACCTTTTTCATGTGTCTTGCCTTCGATCATGGTTAGAGCGACAGCATACCTGCCCTACTTGCAGGGCTCTAGTTGTACCTCCTGACAGTGGGACAGGTACAGCTGGCGGGCATAGGGGCTCACAGTCAGATGCTCATCAACAGGGGACAGGCACAATGGCTCAGGGTGCTGGAGTTGCTGGTGATAGTGTGAGCCAACATGAAGCCAGGCTCCGAGCTGCTGCTGCTGCTGCATCAATATATGAGAAGTCTCATGTATATGCTTCTGCAAACAGACTAGTATGGTCTCCTGGATATGCGGCACTTCCTCAGGTTCAAAGACATACGGCTGACTCCCCTAATACTGAATCTAGTGGAGAAGCGTCTAGTGGACAATCACGGCCTCAGCTTGCAACCCCTGCTGGAGCTGCAAACTTGTCCTTCTCTCAGTTTCCACCTTACATGTTTGTTCCTGGCGCCAATGTGGCCTATGGGGGGAGGCTGAGCAGCGATCAAAATCTGACAGCTTCTCAGCTGGAGGCCCAGAAAAAGTTTCTTCAGGCCCAGATAGGGATACTGCAGAATCAGCTCCAAGTTTTACAGAAACCAAAACCAAAGGAAAGTGTGGACATGGGTCCAACAGGGGCATCTTTGGATGGCAAAGGAAAGAGCGTTGCAGCTCCTTCATCTGTTTCAGATTGTGGACGTCATGAAGATCGGGTTGATACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000153 GO:0000209 GO:0000835 GO:0000836 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0023052 GO:0030163 GO:0030433 GO:0030968 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034620 GO:0034645 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070085 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071944 GO:0098796 GO:0098827 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1902494 GO:1990234 GO:1990381
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 map04120 map04141
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

545

Amino Acids

60.55

Weight (kDa)

8.79

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_SYVN1_N PF25563 3 - 272 4.2e-108 E3 ubiquitin-protein ligase synoviolin-like, TPR repeats
zf-rbx1 PF12678 287 - 330 9e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 291 - 330 5e-11 Ring finger domain
zf-C3HC4 PF00097 292 - 329 4.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 292 - 327 9.5e-07 RING-type zinc-finger
zf-C3HC4_2 PF13923 292 - 329 2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
AatII GACGTC 2 cut(s) 116, 1612
Acc36I ACCTGC 1 cut(s) 983
AccB1I GGYRCC 1 cut(s) 1379
AccI GTMKAC 2 cut(s) 110, 633
AccII CGCG 1 cut(s) 701
AciI CCGC 4 cut(s) 23, 247, 1032, 1223
AclI AACGTT 1 cut(s) 566
AclWI GGATC 1 cut(s) 208
AcuI CTGAAG 2 cut(s) 867, 1445
AcyI GRCGYC 3 cut(s) 113, 1380, 1609
AfaI GTAC 7 cut(s) 166, 377, 470, 536, 713, 1002, 1024
AfiI CCNNNNNNNGG 4 cut(s) 24, 1013, 1398, 1473
AflII CTTAAG 1 cut(s) 602
AflIII ACRYGT 2 cut(s) 636, 1365
AgsI TTSAA 1 cut(s) 1241
AhlI ACTAGT 1 cut(s) 1201
AjnI CCWGG 3 cut(s) 1130, 1213, 1375
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
Alw26I GTCTC 7 cut(s) 307, 377, 414, 430, 464, 1179, 1215
AlwI GGATC 1 cut(s) 208
AoxI GGCC 5 cut(s) 504, 1304, 1389, 1443, 1464
ApeKI GCWGC 9 cut(s) 10, 1142, 1145, 1148, 1151, 1154, 1331, 1410, 1580
Asp700I GAANNNNTTC 1 cut(s) 654
AspLEI GCGC 1 cut(s) 1382
AspS9I GGNCC 3 cut(s) 1444, 1465, 1538
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 1 cut(s) 1118
AvaII GGWCC 1 cut(s) 1538
AxyI CCTNAGG 1 cut(s) 1233
BaeGI GKGCMC 1 cut(s) 402
BanI GGYRCC 1 cut(s) 1379
BanII GRGCYC 2 cut(s) 994, 1046
BarI GAAGNNNNNNTAC 4 cut(s) 617, 649, 674, 706
BbvCI CCTCAGC 1 cut(s) 1308
BbvI GCAGC 8 cut(s) 1129, 1132, 1135, 1138, 1141, 1318, 1422, 1592
BccI CCATC 2 cut(s) 281, 1553
BceAI ACGGC 3 cut(s) 870, 1266, 1319
BciT130I CCWGG 3 cut(s) 1132, 1215, 1377
BciVI GTATCC 1 cut(s) 1470
BclI TGATCA 1 cut(s) 336
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 7 cut(s) 307, 377, 414, 430, 464, 1179, 1215
BcuI ACTAGT 1 cut(s) 1201
BfaI CTAG 7 cut(s) 47, 452, 545, 995, 1202, 1275, 1290
BfmI CTRYAG 1 cut(s) 1481
BfoI RGCGCY 1 cut(s) 1383
BfrI CTTAAG 1 cut(s) 602
BfuAI ACCTGC 1 cut(s) 983
BfuI GTATCC 1 cut(s) 1470
BglI GCCNNNNNGGC 1 cut(s) 1388
BglII AGATCT 1 cut(s) 189
BlpI GCTNAGC 1 cut(s) 1406
Bme1390I CCNGG 4 cut(s) 209, 1132, 1215, 1377
Bme18I GGWCC 1 cut(s) 1538
BmgT120I GGNCC 3 cut(s) 1444, 1465, 1538
BmiI GGNNCC 3 cut(s) 1136, 1381, 1539
BmrFI CCNGG 4 cut(s) 209, 1132, 1215, 1377
BmsI GCATC 4 cut(s) 826, 1045, 1166, 1559
BoxI GACNNNNGTC 1 cut(s) 1536
BpmI CTGGAG 3 cut(s) 1116, 1347, 1460
Bpu10I CCTNAGC 2 cut(s) 1086, 1308
Bpu1102I GCTNAGC 1 cut(s) 1406
BpuMI CCSGG 1 cut(s) 209
Bsa29I ATCGAT 1 cut(s) 826
BsaAI YACGTR 3 cut(s) 711, 740, 1632
BsaBI GATNNNNATC 2 cut(s) 1059, 1419
BsaHI GRCGYC 3 cut(s) 113, 1380, 1609
BsaI GGTCTC 2 cut(s) 307, 1215
BsaJI CCNNGG 3 cut(s) 196, 216, 507
BsaXI ACNNNNNCTCC 2 cut(s) 599, 629
Bsc4I CCNNNNNNNGG 4 cut(s) 24, 1013, 1398, 1473
Bse21I CCTNAGG 1 cut(s) 1233
Bse3DI GCAATG 2 cut(s) 102, 777
Bse8I GATNNNNATC 2 cut(s) 1059, 1419
BseBI CCWGG 3 cut(s) 1132, 1215, 1377
BseCI ATCGAT 1 cut(s) 826
BseDI CCNNGG 3 cut(s) 196, 216, 507
BseGI GGATG 1 cut(s) 1564
BseJI GATNNNNATC 2 cut(s) 1059, 1419
BseLI CCNNNNNNNGG 4 cut(s) 24, 1013, 1398, 1473
BseMI GCAATG 2 cut(s) 102, 777
BseMII CTCAG 7 cut(s) 374, 1100, 1247, 1322, 1364, 1397, 1448
BseSI GKGCMC 1 cut(s) 402
BseXI GCAGC 8 cut(s) 1129, 1132, 1135, 1138, 1141, 1318, 1422, 1592
BsgI GTGCAG 1 cut(s) 713
Bsh1236I CGCG 1 cut(s) 701
BshFI GGCC 5 cut(s) 506, 1306, 1391, 1445, 1466
BshNI GGYRCC 1 cut(s) 1379
BshVI ATCGAT 1 cut(s) 826
BsiSI CCGG 2 cut(s) 92, 208
BslFI GGGAC 4 cut(s) 624, 674, 1030, 1084
BslI CCNNNNNNNGG 4 cut(s) 24, 1013, 1398, 1473
BsmAI GTCTC 7 cut(s) 307, 377, 414, 430, 464, 1179, 1215
BsmFI GGGAC 4 cut(s) 624, 674, 1030, 1084
BsnI GGCC 5 cut(s) 506, 1306, 1391, 1445, 1466
Bso31I GGTCTC 2 cut(s) 307, 1215
Bsp1286I GDGCHC 3 cut(s) 402, 994, 1046
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 8 cut(s) 120, 189, 200, 336, 823, 950, 1414, 1618
Bsp1720I GCTNAGC 1 cut(s) 1406
Bsp68I TCGCGA 1 cut(s) 701
BspACI CCGC 4 cut(s) 23, 247, 1032, 1223
BspANI GGCC 5 cut(s) 506, 1306, 1391, 1445, 1466
BspCNI CTCAG 7 cut(s) 373, 1099, 1246, 1321, 1363, 1398, 1447
BspDI ATCGAT 1 cut(s) 826
BspFNI CGCG 1 cut(s) 701
BspHI TCATGA 1 cut(s) 1612
BspLI GGNNCC 3 cut(s) 1136, 1381, 1539
BspMAI CTGCAG 1 cut(s) 1485
BspMI ACCTGC 1 cut(s) 983
BspPI GGATC 1 cut(s) 208
BspQI GCTCTTC 1 cut(s) 843
BspT107I GGYRCC 1 cut(s) 1379
BspTI CTTAAG 1 cut(s) 602
BspTNI GGTCTC 2 cut(s) 307, 1215
BsrDI GCAATG 2 cut(s) 102, 777
BsrGI TGTACA 1 cut(s) 468
BssECI CCNNGG 3 cut(s) 196, 216, 507
BssMI GATC 8 cut(s) 120, 189, 200, 336, 823, 950, 1414, 1618
BssNI GRCGYC 3 cut(s) 113, 1380, 1609
BssT1I CCWWGG 1 cut(s) 196
Bst2UI CCWGG 3 cut(s) 1132, 1215, 1377
Bst4CI ACNGT 5 cut(s) 473, 559, 615, 1013, 1050
Bst6I CTCTTC 4 cut(s) 359, 524, 843, 885
BstACI GRCGYC 3 cut(s) 113, 1380, 1609
BstAFI CTTAAG 1 cut(s) 602
BstAPI GCANNNNNTGC 1 cut(s) 1322
BstAUI TGTACA 1 cut(s) 468
BstBAI YACGTR 3 cut(s) 711, 740, 1632
BstC8I GCNNGC 3 cut(s) 1030, 1034, 1314
BstDEI CTNAG 7 cut(s) 360, 1086, 1233, 1308, 1350, 1406, 1434
BstF5I GGATG 1 cut(s) 1564
BstFNI CGCG 1 cut(s) 701
BstH2I RGCGCY 1 cut(s) 1383
BstHHI GCGC 1 cut(s) 1382
BstKTI GATC 8 cut(s) 123, 192, 203, 339, 826, 953, 1417, 1621
BstMAI GTCTC 7 cut(s) 307, 377, 414, 430, 464, 1179, 1215
BstMBI GATC 8 cut(s) 120, 189, 200, 336, 823, 950, 1414, 1618
BstNI CCWGG 3 cut(s) 1132, 1215, 1377
BstNSI RCATGY 2 cut(s) 601, 1369
BstPAI GACNNNNGTC 1 cut(s) 1536
BstSCI CCNGG 4 cut(s) 207, 1130, 1213, 1375
BstSFI CTRYAG 1 cut(s) 1481
BstSLI GKGCMC 1 cut(s) 402
BstSNI TACGTA 1 cut(s) 1632
BstUI CGCG 1 cut(s) 701
BstV1I GCAGC 8 cut(s) 1129, 1132, 1135, 1138, 1141, 1318, 1422, 1592
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
Bsu15I ATCGAT 1 cut(s) 826
Bsu36I CCTNAGG 1 cut(s) 1233
BsuI GTATCC 1 cut(s) 1470
BsuRI GGCC 5 cut(s) 506, 1306, 1391, 1445, 1466
BsuTUI ATCGAT 1 cut(s) 826
BtsCI GGATG 1 cut(s) 1564
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 4 cut(s) 564, 620, 725, 1018
BtuMI TCGCGA 1 cut(s) 701
BveI ACCTGC 1 cut(s) 983
Cac8I GCNNGC 3 cut(s) 1030, 1034, 1314
CciI TCATGA 1 cut(s) 1612
CfoI GCGC 1 cut(s) 1382
Cfr13I GGNCC 3 cut(s) 1444, 1465, 1538
ClaI ATCGAT 1 cut(s) 826
CseI GACGC 2 cut(s) 875, 1275
Csp6I GTAC 7 cut(s) 165, 376, 469, 535, 712, 1001, 1023
CspCI CAANNNNNGTGG 2 cut(s) 714, 749
CviQI GTAC 7 cut(s) 165, 376, 469, 535, 712, 1001, 1023
DdeI CTNAG 7 cut(s) 360, 1086, 1233, 1308, 1350, 1406, 1434
DinI GGCGCC 1 cut(s) 1381
DpnI GATC 8 cut(s) 122, 191, 202, 338, 825, 952, 1416, 1620
DpnII GATC 8 cut(s) 120, 189, 200, 336, 823, 950, 1414, 1618
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
Eam1104I CTCTTC 4 cut(s) 359, 524, 843, 885
EarI CTCTTC 4 cut(s) 359, 524, 843, 885
Eco105I TACGTA 1 cut(s) 1632
Eco130I CCWWGG 1 cut(s) 196
Eco147I AGGCCT 1 cut(s) 506
Eco24I GRGCYC 2 cut(s) 994, 1046
Eco31I GGTCTC 2 cut(s) 307, 1215
Eco47I GGWCC 1 cut(s) 1538
Eco57I CTGAAG 2 cut(s) 867, 1445
Eco81I CCTNAGG 1 cut(s) 1233
EcoRII CCWGG 3 cut(s) 1130, 1213, 1375
EcoT14I CCWWGG 1 cut(s) 196
EcoT22I ATGCAT 1 cut(s) 67
EcoT38I GRGCYC 2 cut(s) 994, 1046
EgeI GGCGCC 1 cut(s) 1381
EheI GGCGCC 1 cut(s) 1381
ErhI CCWWGG 1 cut(s) 196
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 4 cut(s) 624, 674, 1030, 1084
FauI CCCGC 3 cut(s) 16, 240, 1025
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 2 cut(s) 110, 633
FokI GGATG 1 cut(s) 1571
FriOI GRGCYC 2 cut(s) 994, 1046
FspBI CTAG 7 cut(s) 47, 452, 545, 995, 1202, 1275, 1290
GlaI GCGC 1 cut(s) 1381
GsuI CTGGAG 3 cut(s) 1116, 1347, 1460
HaeII RGCGCY 1 cut(s) 1383
HaeIII GGCC 5 cut(s) 506, 1306, 1391, 1445, 1466
HapII CCGG 2 cut(s) 92, 208
HgaI GACGC 2 cut(s) 875, 1275
HhaI GCGC 1 cut(s) 1382
Hin1I GRCGYC 3 cut(s) 113, 1380, 1609
Hin6I GCGC 1 cut(s) 1380
HinP1I GCGC 1 cut(s) 1380
HincII GTYRAC 2 cut(s) 111, 564
HindII GTYRAC 2 cut(s) 111, 564
HindIII AAGCTT 1 cut(s) 770
HinfI GANTC 4 cut(s) 828, 1256, 1271, 1486
HpaII CCGG 2 cut(s) 92, 208
HphI GGTGA 1 cut(s) 1118
Hpy166II GTNNAC 8 cut(s) 111, 165, 564, 634, 923, 1295, 1531, 1607
Hpy188I TCNGA 6 cut(s) 363, 420, 1054, 1139, 1425, 1600
Hpy188III TCNNGA 6 cut(s) 382, 452, 659, 700, 1007, 1613
Hpy8I GTNNAC 8 cut(s) 111, 165, 564, 634, 923, 1295, 1531, 1607
Hpy99I CGWCG 2 cut(s) 115, 118
HpyAV CCTTC 4 cut(s) 402, 956, 1354, 1596
HpyCH4III ACNGT 5 cut(s) 473, 559, 615, 1013, 1050
HpyCH4IV ACGT 7 cut(s) 113, 566, 638, 710, 739, 1609, 1631
HpyF3I CTNAG 7 cut(s) 360, 1086, 1233, 1308, 1350, 1406, 1434
HpySE526I ACGT 7 cut(s) 113, 566, 638, 710, 739, 1609, 1631
Hsp92I GRCGYC 3 cut(s) 113, 1380, 1609
HspAI GCGC 1 cut(s) 1380
KasI GGCGCC 1 cut(s) 1379
Ksp22I TGATCA 1 cut(s) 336
Kzo9I GATC 8 cut(s) 120, 189, 200, 336, 823, 950, 1414, 1618
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 4 cut(s) 1140, 1328, 1497, 1588
Lsp1109I GCAGC 8 cut(s) 1129, 1132, 1135, 1138, 1141, 1318, 1422, 1592
LweI GCATC 4 cut(s) 826, 1045, 1166, 1559
MaeI CTAG 7 cut(s) 47, 452, 545, 995, 1202, 1275, 1290
MaeII ACGT 7 cut(s) 113, 566, 638, 710, 739, 1609, 1631
MaeIII GTNAC 3 cut(s) 319, 589, 863
MalI GATC 8 cut(s) 122, 191, 202, 338, 825, 952, 1416, 1620
MboI GATC 8 cut(s) 120, 189, 200, 336, 823, 950, 1414, 1618
MfeI CAATTG 1 cut(s) 176
MflI RGATCY 1 cut(s) 189
MhlI GDGCHC 3 cut(s) 402, 994, 1046
MluCI AATT 1 cut(s) 176
Mly113I GGCGCC 1 cut(s) 1380
MlyI GAGTC 1 cut(s) 1250
MmeI TCCRAC 1 cut(s) 1565
Mph1103I ATGCAT 1 cut(s) 67
MroXI GAANNNNTTC 1 cut(s) 654
MseI TTAA 4 cut(s) 231, 603, 768, 855
MslI CAYNNNNRTG 1 cut(s) 1182
MspA1I CMGCKG 2 cut(s) 1028, 1438
MspCI CTTAAG 1 cut(s) 602
MspI CCGG 2 cut(s) 92, 208
MspR9I CCNGG 4 cut(s) 209, 1132, 1215, 1377
MunI CAATTG 1 cut(s) 176
MvaI CCWGG 3 cut(s) 1132, 1215, 1377
MvnI CGCG 1 cut(s) 701
NarI GGCGCC 1 cut(s) 1380
NciI CCSGG 1 cut(s) 209
NdeII GATC 8 cut(s) 120, 189, 200, 336, 823, 950, 1414, 1618
NlaIV GGNNCC 3 cut(s) 1136, 1381, 1539
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 1 cut(s) 863
NruI TCGCGA 1 cut(s) 701
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 2 cut(s) 601, 1369
PagI TCATGA 1 cut(s) 1612
PceI AGGCCT 1 cut(s) 506
PciI ACATGT 1 cut(s) 1365
PciSI GCTCTTC 1 cut(s) 843
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 3 cut(s) 828, 1271, 1486
PfoI TCCNGGA 2 cut(s) 207, 1213
PleI GAGTC 1 cut(s) 1250
PluTI GGCGCC 1 cut(s) 1383
PpsI GAGTC 1 cut(s) 1250
Ppu21I YACGTR 3 cut(s) 711, 740, 1632
PscI ACATGT 1 cut(s) 1365
PshAI GACNNNNGTC 1 cut(s) 1536
Psp1406I AACGTT 1 cut(s) 566
Psp6I CCWGG 3 cut(s) 1130, 1213, 1375
PspGI CCWGG 3 cut(s) 1130, 1213, 1375
PspN4I GGNNCC 3 cut(s) 1136, 1381, 1539
PspPI GGNCC 3 cut(s) 1444, 1465, 1538
PstI CTGCAG 1 cut(s) 1485
PsuI RGATCY 1 cut(s) 189
PvuII CAGCTG 2 cut(s) 1028, 1438
RruI TCGCGA 1 cut(s) 701
RsaI GTAC 7 cut(s) 166, 377, 470, 536, 713, 1002, 1024
RsaNI GTAC 7 cut(s) 165, 376, 469, 535, 712, 1001, 1023
RseI CAYNNNNRTG 1 cut(s) 1182
SalI GTCGAC 1 cut(s) 109
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 4 cut(s) 231, 603, 768, 855
Sau3AI GATC 8 cut(s) 120, 189, 200, 336, 823, 950, 1414, 1618
Sau96I GGNCC 3 cut(s) 1444, 1465, 1538
SchI GAGTC 1 cut(s) 1250
ScrFI CCNGG 4 cut(s) 209, 1132, 1215, 1377
SduI GDGCHC 3 cut(s) 402, 994, 1046
SfaNI GCATC 4 cut(s) 826, 1045, 1166, 1559
SfcI CTRYAG 1 cut(s) 1481
SfoI GGCGCC 1 cut(s) 1381
SinI GGWCC 1 cut(s) 1538
SmiMI CAYNNNNRTG 1 cut(s) 1182
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
SnaBI TACGTA 1 cut(s) 1632
SpeI ACTAGT 1 cut(s) 1201
Sse9I AATT 1 cut(s) 176
SseBI AGGCCT 1 cut(s) 506
SsiI CCGC 4 cut(s) 23, 247, 1032, 1223
SspDI GGCGCC 1 cut(s) 1379
SspMI CTAG 7 cut(s) 47, 452, 545, 995, 1202, 1275, 1290
StuI AGGCCT 1 cut(s) 506
StyD4I CCNGG 4 cut(s) 207, 1130, 1213, 1375
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 5 cut(s) 473, 559, 615, 1013, 1050
TaiI ACGT 7 cut(s) 116, 569, 641, 713, 742, 1612, 1634
TaqI TCGA 7 cut(s) 110, 116, 381, 531, 799, 826, 949
TaqII GACCGA 1 cut(s) 289
TasI AATT 1 cut(s) 176
TatI WGTACW 4 cut(s) 164, 375, 468, 534
TauI GCSGC 1 cut(s) 1226
TfiI GAWTC 3 cut(s) 828, 1271, 1486
Tru1I TTAA 4 cut(s) 231, 603, 768, 855
Tru9I TTAA 4 cut(s) 231, 603, 768, 855
TscAI CASTG 4 cut(s) 564, 620, 732, 1018
TseFI GTSAC 1 cut(s) 863
TseI GCWGC 9 cut(s) 10, 1142, 1145, 1148, 1151, 1154, 1331, 1410, 1580
Tsp45I GTSAC 1 cut(s) 863
TspDTI ATGAA 9 cut(s) 20, 516, 659, 765, 833, 923, 1140, 1578, 1629
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 4 cut(s) 564, 620, 732, 1018
Vha464I CTTAAG 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 1538
XbaI TCTAGA 1 cut(s) 451
XceI RCATGY 2 cut(s) 601, 1369
XmiI GTMKAC 2 cut(s) 110, 633
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 7 cut(s) 47, 452, 545, 995, 1202, 1275, 1290
ZraI GACGTC 2 cut(s) 114, 1610
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.