Rh1AG470000

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003784
Physical Location & Seq
Reverse (-)
68299888 .. 68306264
6377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG470000.1

Sequence Viewer

Length: 1617 bp
ATGATGAGGCTGCAAACTTACGCGGGGCTTAGCTTGATAGGAACCCTAGCGATTATTTACCATGCATTTAGCAGCAGAGGCCAGTTTTACCCCGCCATGGTTTACCTGTCAACCTCCAAGATCAGTCTGGTGCTTCTTCTCAACATGGGCCTTGTCATCATGTGTATTTTGTGGCAACTGACTAAGAAGCTATTCCTCGGCTCACTCCGAGAGCCGGAGGTTGAGAGGCTTAATGAGCAGTCGTGGAGGGAAGTCATGGAGATTCTCTTTGCCATCACTATTTTCCGGCAGGACTTTTCGGTGTCGTTTCTGGCTTTTGTCACTGCTCTGTTGCTGATCAAGGCGCTGCATTGGTTGGCGCAGAAGAGGGTTGAGTACATTGAGACTACTCCGTCTGTTCCCACGCTGTCGCATGTTCGGATTGTCTCCTTTTTGGGGTTCTTGTTTGTTGTAGATAGTCTCTTTTTGTATACTTCTGTCAGCACCATCATTACGACTCAGAAGCCTTCGGTTTCCCTCTTTTTTGCATTCGAGTACATGATCCTAGCAACAACAACAACACTGTCAACATTTGTGAAATATGTTTTCTATGTTAATGACATGATTATGGAGGGACAATGGGAGAGGAAGCATGTCTACACCTTCTATTTGGAACTTATTCGGGACTTGCTCCACTTATCTATGTATTTGTGCTTCTTCATCGTGATTTTCATGTACTATGGCTTGCCAGTGCACTTGATTCGCGAACTGTCTGAGACGTACAGGAACTTTAAGATTCGTGTTGCTGATTACCTCCGTTATCGAAAGATCACTTCAAACATGAATGACCGGTTCCCAGACGCTACACCTGAAGAGCTTACTGCAGGTGACGCAACCTGCATCATTTGTCGTGAAGAGATGACAACAGCCAAAAAACTATTATGTGGACACCTTTTTCATGTGGCTTGCTTGCGATCATGGTTAGAGCGACAACATACCTGTCCTACATGCCGAGCGCTTGTTGTACCTCCTGATAGTCGGACAGGTACAGCTCGAGGACAAGGTGGATCACGCTCCGATGCTCATCAACAGGGGACAGGCATATCAGGTACAACTGCTCAAGGTTCAACTAGTGGTGGAGGTGCTGGTGATAACATGAGCCATCATGAAGCAAGACTGCGAGCTGCTGCTGCAGCTGCATCAATATATGAGAAATCTTATGTATATCCATCTGCAAACAGACTAGTATGGTCTGCTGGAGATGCCGCACTTCCTCTGGTTAATAGACATATGGCTGACTCCCCTAGTACTGATTTACAATCACAACCACTTGCAACCCCTGGTGGACCTGCAAACTTGTCATTGCCTCAATCTCCGCATTACATGTTTTCTCCTTTTCATGGAGCCAATGGGGAGAGGTCGGGCAGCAATACAAATATAACAGCTTCTCAGCTGGAGGCCCAGAGAAAAGTTCTTCAGCACCAGATTGAGATCCTGCAGAATCAGCTCCAAATTTTACAAAAGCCAAAACCCAAGGAAAGTGTGGAAGGGGGTCCAACATTAGCAGATAGCAAAGGAAAGAGTGTTGCACCTTCGTCTGAATCAAATTGCGGGCGTCATGAGGATGTTGTTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000153 GO:0000209 GO:0000835 GO:0000836 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0023052 GO:0030163 GO:0030433 GO:0030968 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034620 GO:0034645 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070085 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071944 GO:0098796 GO:0098827 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1902494 GO:1990234 GO:1990381
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 map04120 map04141
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

538

Amino Acids

60.19

Weight (kDa)

8.45

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_SYVN1_N PF25563 3 - 273 8.9e-103 E3 ubiquitin-protein ligase synoviolin-like, TPR repeats
zf-rbx1 PF12678 291 - 331 1.2e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 292 - 331 7.9e-11 Ring finger domain
zf-C3HC4 PF00097 293 - 330 7e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 293 - 328 1.3e-06 RING-type zinc-finger
zf-C3HC4_2 PF13923 293 - 330 3.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 854
AasI GACNNNNNNGTC 1 cut(s) 391
Acc36I ACCTGC 3 cut(s) 854, 884, 1336
AccI GTMKAC 2 cut(s) 470, 636
AccII CGCG 2 cut(s) 23, 744
AciI CCGC 5 cut(s) 23, 93, 1245, 1355, 1590
AclWI GGATC 3 cut(s) 535, 1054, 1465
AcsI RAATTY 1 cut(s) 1491
AcuI CTGAAG 2 cut(s) 870, 1439
AcyI GRCGYC 1 cut(s) 1594
AfaI GTAC 8 cut(s) 377, 536, 716, 761, 1005, 1027, 1090, 1288
AfeI AGCGCT 1 cut(s) 996
AfiI CCNNNNNNNGG 4 cut(s) 97, 214, 435, 1379
AflIII ACRYGT 1 cut(s) 1362
AgeI ACCGGT 1 cut(s) 828
AgsI TTSAA 2 cut(s) 816, 1107
AhlI ACTAGT 2 cut(s) 1109, 1222
AjnI CCWGG 1 cut(s) 1318
AluBI AGCT 9 cut(s) 33, 190, 856, 1031, 1163, 1175, 1424, 1432, 1486
AluI AGCT 9 cut(s) 33, 190, 856, 1031, 1163, 1175, 1424, 1432, 1486
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 4 cut(s) 377, 430, 464, 749
Alw44I GTGCAC 1 cut(s) 731
AlwI GGATC 3 cut(s) 535, 1054, 1465
Ama87I CYCGRG 1 cut(s) 1032
Aor51HI AGCGCT 1 cut(s) 996
AoxI GGCC 3 cut(s) 79, 148, 1437
ApaLI GTGCAC 1 cut(s) 731
ApeKI GCWGC 9 cut(s) 10, 72, 346, 1163, 1166, 1169, 1172, 1175, 1404
ApoI RAATTY 1 cut(s) 1491
AsiGI ACCGGT 1 cut(s) 828
Asp700I GAANNNNTTC 2 cut(s) 191, 657
AspLEI GCGC 3 cut(s) 346, 361, 997
AspS9I GGNCC 4 cut(s) 148, 1325, 1438, 1532
AsuHPI GGTGA 2 cut(s) 878, 1139
AvaI CYCGRG 1 cut(s) 1032
AvaII GGWCC 2 cut(s) 1325, 1532
BaeGI GKGCMC 1 cut(s) 735
BarI GAAGNNNNNNTAC 4 cut(s) 620, 652, 677, 709
Bbv12I GWGCWC 1 cut(s) 735
BbvI GCAGC 8 cut(s) 84, 333, 1150, 1153, 1156, 1162, 1184, 1416
BccI CCATC 4 cut(s) 281, 494, 1149, 1216
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 1 cut(s) 1320
BclI TGATCA 1 cut(s) 336
BcoDI GTCTC 4 cut(s) 377, 430, 464, 749
BcuI ACTAGT 2 cut(s) 1109, 1222
BfaI CTAG 6 cut(s) 47, 545, 1110, 1223, 1284, 1615
BfmI CTRYAG 3 cut(s) 861, 1170, 1475
BfoI RGCGCY 2 cut(s) 347, 998
BfuAI ACCTGC 3 cut(s) 854, 884, 1336
BlpI GCTNAGC 1 cut(s) 29
BmcAI AGTACT 1 cut(s) 1288
Bme1390I CCNGG 1 cut(s) 1320
Bme18I GGWCC 2 cut(s) 1325, 1532
BmeT110I CYCGRG 1 cut(s) 1032
BmgT120I GGNCC 4 cut(s) 148, 1325, 1438, 1532
BmiI GGNNCC 4 cut(s) 43, 833, 1384, 1533
BmrFI CCNGG 1 cut(s) 1320
BmsI GCATC 4 cut(s) 888, 1048, 1187, 1231
BpmI CTGGAG 2 cut(s) 1257, 1454
Bpu1102I GCTNAGC 1 cut(s) 29
BpuEI CTTGAG 1 cut(s) 1083
BsaBI GATNNNNATC 2 cut(s) 1062, 1469
BsaHI GRCGYC 1 cut(s) 1594
BsaJI CCNNGG 4 cut(s) 96, 196, 1318, 1512
BsaWI WCCGGW 1 cut(s) 828
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 214, 435, 1379
Bse118I RCCGGY 1 cut(s) 828
Bse1I ACTGG 2 cut(s) 82, 728
Bse3DI GCAATG 1 cut(s) 1340
Bse8I GATNNNNATC 2 cut(s) 1062, 1469
BseBI CCWGG 1 cut(s) 1320
BseDI CCNNGG 4 cut(s) 96, 196, 1318, 1512
BseGI GGATG 1 cut(s) 1609
BseJI GATNNNNATC 2 cut(s) 1062, 1469
BseLI CCNNNNNNNGG 4 cut(s) 97, 214, 435, 1379
BseMI GCAATG 1 cut(s) 1340
BseMII CTCAG 3 cut(s) 512, 744, 1442
BseNI ACTGG 2 cut(s) 82, 728
BseSI GKGCMC 1 cut(s) 735
BseXI GCAGC 8 cut(s) 84, 333, 1150, 1153, 1156, 1162, 1184, 1416
Bsh1236I CGCG 2 cut(s) 23, 744
BshFI GGCC 3 cut(s) 81, 150, 1439
BshTI ACCGGT 1 cut(s) 828
BsiHKAI GWGCWC 1 cut(s) 735
BsiHKCI CYCGRG 1 cut(s) 1032
BsiSI CCGG 3 cut(s) 215, 286, 829
BslFI GGGAC 3 cut(s) 627, 677, 1087
BslI CCNNNNNNNGG 4 cut(s) 97, 214, 435, 1379
BsmAI GTCTC 4 cut(s) 377, 430, 464, 749
BsmBI CGTCTC 1 cut(s) 749
BsmFI GGGAC 3 cut(s) 627, 677, 1087
BsmI GAATGC 1 cut(s) 527
BsnI GGCC 3 cut(s) 81, 150, 1439
BsoBI CYCGRG 1 cut(s) 1032
Bsp1286I GDGCHC 1 cut(s) 735
Bsp143I GATC 7 cut(s) 120, 336, 540, 807, 953, 1046, 1470
Bsp1720I GCTNAGC 1 cut(s) 29
Bsp19I CCATGG 1 cut(s) 96
Bsp68I TCGCGA 1 cut(s) 744
BspACI CCGC 5 cut(s) 23, 93, 1245, 1355, 1590
BspANI GGCC 3 cut(s) 81, 150, 1439
BspCNI CTCAG 3 cut(s) 511, 745, 1441
BspFNI CGCG 2 cut(s) 23, 744
BspHI TCATGA 2 cut(s) 1144, 1597
BspLI GGNNCC 4 cut(s) 43, 833, 1384, 1533
BspMAI CTGCAG 3 cut(s) 865, 1174, 1479
BspMI ACCTGC 3 cut(s) 854, 884, 1336
BspPI GGATC 3 cut(s) 535, 1054, 1465
BspQI GCTCTTC 1 cut(s) 846
BsrDI GCAATG 1 cut(s) 1340
BsrFI RCCGGY 1 cut(s) 828
BsrI ACTGG 2 cut(s) 82, 728
BssAI RCCGGY 1 cut(s) 828
BssECI CCNNGG 4 cut(s) 96, 196, 1318, 1512
BssMI GATC 7 cut(s) 120, 336, 540, 807, 953, 1046, 1470
BssNAI GTATAC 1 cut(s) 471
BssNI GRCGYC 1 cut(s) 1594
BssT1I CCWWGG 2 cut(s) 96, 1512
Bst1107I GTATAC 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 1320
Bst4CI ACNGT 2 cut(s) 564, 750
Bst6I CTCTTC 3 cut(s) 359, 846, 888
BstACI GRCGYC 1 cut(s) 1594
BstC8I GCNNGC 5 cut(s) 725, 946, 950, 1161, 1592
BstDEI CTNAG 5 cut(s) 29, 183, 498, 753, 1428
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 1609
BstFNI CGCG 2 cut(s) 23, 744
BstH2I RGCGCY 2 cut(s) 347, 998
BstHHI GCGC 3 cut(s) 346, 361, 997
BstKTI GATC 7 cut(s) 123, 339, 543, 810, 956, 1049, 1473
BstMAI GTCTC 4 cut(s) 377, 430, 464, 749
BstMBI GATC 7 cut(s) 120, 336, 540, 807, 953, 1046, 1470
BstMWI GCNNNNNNNGC 8 cut(s) 78, 235, 869, 1169, 1172, 1175, 1241, 1483
BstNI CCWGG 1 cut(s) 1320
BstNSI RCATGY 4 cut(s) 416, 635, 990, 1366
BstSCI CCNGG 1 cut(s) 1318
BstSFI CTRYAG 3 cut(s) 861, 1170, 1475
BstSLI GKGCMC 1 cut(s) 735
BstUI CGCG 2 cut(s) 23, 744
BstV1I GCAGC 8 cut(s) 84, 333, 1150, 1153, 1156, 1162, 1184, 1416
BstX2I RGATCY 1 cut(s) 1470
BstYI RGATCY 1 cut(s) 1470
BstZ17I GTATAC 1 cut(s) 471
BsuRI GGCC 3 cut(s) 81, 150, 1439
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 1609
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 3 cut(s) 321, 560, 735
BtuMI TCGCGA 1 cut(s) 744
BveI ACCTGC 3 cut(s) 854, 884, 1336
Cac8I GCNNGC 5 cut(s) 725, 946, 950, 1161, 1592
CciI TCATGA 2 cut(s) 1144, 1597
CfoI GCGC 3 cut(s) 346, 361, 997
Cfr10I RCCGGY 1 cut(s) 828
Cfr13I GGNCC 4 cut(s) 148, 1325, 1438, 1532
CseI GACGC 3 cut(s) 848, 878, 1583
Csp6I GTAC 8 cut(s) 376, 535, 715, 760, 1004, 1026, 1089, 1287
CspAI ACCGGT 1 cut(s) 828
CviQI GTAC 8 cut(s) 376, 535, 715, 760, 1004, 1026, 1089, 1287
DdeI CTNAG 5 cut(s) 29, 183, 498, 753, 1428
DpnI GATC 7 cut(s) 122, 338, 542, 809, 955, 1048, 1472
DpnII GATC 7 cut(s) 120, 336, 540, 807, 953, 1046, 1470
DrdI GACNNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 1 cut(s) 391
Eam1104I CTCTTC 3 cut(s) 359, 846, 888
EarI CTCTTC 3 cut(s) 359, 846, 888
Eco130I CCWWGG 2 cut(s) 96, 1512
Eco47I GGWCC 2 cut(s) 1325, 1532
Eco47III AGCGCT 1 cut(s) 996
Eco57I CTGAAG 2 cut(s) 870, 1439
Eco88I CYCGRG 1 cut(s) 1032
EcoRII CCWGG 1 cut(s) 1318
EcoT14I CCWWGG 2 cut(s) 96, 1512
EcoT22I ATGCAT 1 cut(s) 67
ErhI CCWWGG 2 cut(s) 96, 1512
Esp3I CGTCTC 1 cut(s) 749
FaqI GGGAC 3 cut(s) 627, 677, 1087
FauI CCCGC 3 cut(s) 16, 100, 1583
FauNDI CATATG 1 cut(s) 1269
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 2 cut(s) 470, 636
FspBI CTAG 6 cut(s) 47, 545, 1110, 1223, 1284, 1615
GlaI GCGC 3 cut(s) 345, 360, 996
GsuI CTGGAG 2 cut(s) 1257, 1454
HaeII RGCGCY 2 cut(s) 347, 998
HaeIII GGCC 3 cut(s) 81, 150, 1439
HapII CCGG 3 cut(s) 215, 286, 829
HgaI GACGC 3 cut(s) 848, 878, 1583
HhaI GCGC 3 cut(s) 346, 361, 997
Hin1I GRCGYC 1 cut(s) 1594
Hin6I GCGC 3 cut(s) 344, 359, 995
HinP1I GCGC 3 cut(s) 344, 359, 995
HincII GTYRAC 3 cut(s) 111, 567, 1612
HindII GTYRAC 3 cut(s) 111, 567, 1612
HinfI GANTC 7 cut(s) 262, 496, 739, 775, 1277, 1480, 1580
HpaII CCGG 3 cut(s) 215, 286, 829
HphI GGTGA 2 cut(s) 878, 1139
Hpy166II GTNNAC 9 cut(s) 103, 111, 471, 567, 637, 733, 926, 1325, 1612
Hpy188I TCNGA 7 cut(s) 209, 420, 501, 754, 1020, 1057, 1579
Hpy188III TCNNGA 7 cut(s) 662, 703, 743, 890, 1010, 1145, 1598
Hpy8I GTNNAC 9 cut(s) 103, 111, 471, 567, 637, 733, 926, 1325, 1612
HpyAV CCTTC 4 cut(s) 516, 652, 1520, 1581
HpyCH4III ACNGT 2 cut(s) 564, 750
HpyCH4IV ACGT 1 cut(s) 758
HpyF10VI GCNNNNNNNGC 8 cut(s) 78, 235, 869, 1169, 1172, 1175, 1241, 1483
HpyF3I CTNAG 5 cut(s) 29, 183, 498, 753, 1428
HpySE526I ACGT 1 cut(s) 758
Hsp92I GRCGYC 1 cut(s) 1594
HspAI GCGC 3 cut(s) 344, 359, 995
Ksp22I TGATCA 1 cut(s) 336
Kzo9I GATC 7 cut(s) 120, 336, 540, 807, 953, 1046, 1470
LguI GCTCTTC 1 cut(s) 846
LmnI GCTCC 4 cut(s) 675, 1058, 1382, 1491
Lsp1109I GCAGC 8 cut(s) 84, 333, 1150, 1153, 1156, 1162, 1184, 1416
LweI GCATC 4 cut(s) 888, 1048, 1187, 1231
MaeI CTAG 6 cut(s) 47, 545, 1110, 1223, 1284, 1615
MaeII ACGT 1 cut(s) 758
MaeIII GTNAC 2 cut(s) 319, 866
MalI GATC 7 cut(s) 122, 338, 542, 809, 955, 1048, 1472
MboI GATC 7 cut(s) 120, 336, 540, 807, 953, 1046, 1470
MboII GAAGA 6 cut(s) 128, 376, 688, 863, 905, 1445
MflI RGATCY 1 cut(s) 1470
MhlI GDGCHC 1 cut(s) 735
MluCI AATT 2 cut(s) 1491, 1585
MlyI GAGTC 2 cut(s) 490, 1271
MmeI TCCRAC 2 cut(s) 998, 1559
Mph1103I ATGCAT 1 cut(s) 67
MroXI GAANNNNTTC 2 cut(s) 191, 657
MseI TTAA 4 cut(s) 231, 594, 771, 1260
MslI CAYNNNNRTG 2 cut(s) 605, 1602
MspA1I CMGCKG 2 cut(s) 1175, 1432
MspI CCGG 3 cut(s) 215, 286, 829
MspR9I CCNGG 1 cut(s) 1320
Mva1269I GAATGC 1 cut(s) 527
MvaI CCWGG 1 cut(s) 1320
MvnI CGCG 2 cut(s) 23, 744
MwoI GCNNNNNNNGC 8 cut(s) 78, 235, 869, 1169, 1172, 1175, 1241, 1483
NcoI CCATGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 1269
NdeII GATC 7 cut(s) 120, 336, 540, 807, 953, 1046, 1470
NlaIV GGNNCC 4 cut(s) 43, 833, 1384, 1533
NmeAIII GCCGAG 2 cut(s) 177, 1016
NmuCI GTSAC 2 cut(s) 319, 866
NruI TCGCGA 1 cut(s) 744
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 4 cut(s) 416, 635, 990, 1366
PaeR7I CTCGAG 1 cut(s) 1032
PagI TCATGA 2 cut(s) 1144, 1597
PaqCI CACCTGC 1 cut(s) 854
PciI ACATGT 1 cut(s) 1362
PciSI GCTCTTC 1 cut(s) 846
PctI GAATGC 1 cut(s) 527
PdmI GAANNNNTTC 2 cut(s) 191, 657
PfeI GAWTC 5 cut(s) 262, 739, 775, 1480, 1580
PinAI ACCGGT 1 cut(s) 828
PleI GAGTC 2 cut(s) 490, 1271
PpsI GAGTC 2 cut(s) 490, 1271
PscI ACATGT 1 cut(s) 1362
Psp6I CCWGG 1 cut(s) 1318
PspGI CCWGG 1 cut(s) 1318
PspN4I GGNNCC 4 cut(s) 43, 833, 1384, 1533
PspPI GGNCC 4 cut(s) 148, 1325, 1438, 1532
PspXI VCTCGAGB 1 cut(s) 1032
PstI CTGCAG 3 cut(s) 865, 1174, 1479
PsuI RGATCY 1 cut(s) 1470
PvuII CAGCTG 2 cut(s) 1175, 1432
RruI TCGCGA 1 cut(s) 744
RsaI GTAC 8 cut(s) 377, 536, 716, 761, 1005, 1027, 1090, 1288
RsaNI GTAC 8 cut(s) 376, 535, 715, 760, 1004, 1026, 1089, 1287
RseI CAYNNNNRTG 2 cut(s) 605, 1602
SapI GCTCTTC 1 cut(s) 846
SaqAI TTAA 4 cut(s) 231, 594, 771, 1260
Sau3AI GATC 7 cut(s) 120, 336, 540, 807, 953, 1046, 1470
Sau96I GGNCC 4 cut(s) 148, 1325, 1438, 1532
ScaI AGTACT 1 cut(s) 1288
SchI GAGTC 2 cut(s) 490, 1271
ScrFI CCNGG 1 cut(s) 1320
SduI GDGCHC 1 cut(s) 735
SfaNI GCATC 4 cut(s) 888, 1048, 1187, 1231
SfcI CTRYAG 3 cut(s) 861, 1170, 1475
Sfr274I CTCGAG 1 cut(s) 1032
SinI GGWCC 2 cut(s) 1325, 1532
SlaI CTCGAG 1 cut(s) 1032
SmiMI CAYNNNNRTG 2 cut(s) 605, 1602
SmlI CTYRAG 2 cut(s) 1032, 1098
SmoI CTYRAG 2 cut(s) 1032, 1098
SpeI ACTAGT 2 cut(s) 1109, 1222
Sse9I AATT 2 cut(s) 1491, 1585
SsiI CCGC 5 cut(s) 23, 93, 1245, 1355, 1590
SspMI CTAG 6 cut(s) 47, 545, 1110, 1223, 1284, 1615
StyD4I CCNGG 1 cut(s) 1318
StyI CCWWGG 2 cut(s) 96, 1512
TaaI ACNGT 2 cut(s) 564, 750
TaiI ACGT 1 cut(s) 761
TaqI TCGA 3 cut(s) 531, 802, 1033
TasI AATT 2 cut(s) 1491, 1585
TatI WGTACW 4 cut(s) 375, 534, 714, 1286
TauI GCSGC 1 cut(s) 1247
TfiI GAWTC 5 cut(s) 262, 739, 775, 1480, 1580
Tru1I TTAA 4 cut(s) 231, 594, 771, 1260
Tru9I TTAA 4 cut(s) 231, 594, 771, 1260
TscAI CASTG 3 cut(s) 328, 567, 735
TseFI GTSAC 2 cut(s) 319, 866
TseI GCWGC 9 cut(s) 10, 72, 346, 1163, 1166, 1169, 1172, 1175, 1404
Tsp45I GTSAC 2 cut(s) 319, 866
TspDTI ATGAA 6 cut(s) 688, 700, 836, 926, 1161, 1367
TspGWI ACGGA 2 cut(s) 381, 785
TspRI CASTG 3 cut(s) 328, 567, 735
VneI GTGCAC 1 cut(s) 731
VpaK11BI GGWCC 2 cut(s) 1325, 1532
XapI RAATTY 1 cut(s) 1491
XceI RCATGY 4 cut(s) 416, 635, 990, 1366
XcmI CCANNNNNNNNNTGG 2 cut(s) 124, 1519
XhoI CTCGAG 1 cut(s) 1032
XmiI GTMKAC 2 cut(s) 470, 636
XmnI GAANNNNTTC 2 cut(s) 191, 657
XspI CTAG 6 cut(s) 47, 545, 1110, 1223, 1284, 1615
ZrmI AGTACT 1 cut(s) 1288
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.