RchiOBHm_Chr1g0384081

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003784
Physical Location & Seq
Reverse (-)
68109021 .. 68123552
14532 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60693

Sequence Viewer

Length: 939 bp
ATGGTTTACCTATCGACCTCCAAGATCAGTCTGGTGCTTCTTCTCAACATGGGCCTTGTCATCATGTGTATTTTGTGGCAACTGACTAAGAAGCTATTCCTCGGCTCACTCCGAGAGCCGGAGGTTGAGAGGCTTAATGAGCAGTCGTGGAGGGAAGTCATGGAGATTCTTTTCGCAATCACTATTTTCCGGCAGGACTTTTCGGTGTCGTTTCTGGCTTTTGTCACTGCTCTGTTGCTGATCAAGGCGTTGCATTGGTTGGCGCAGAAGAGGGTTGAGTACATTGAGACTACTCCGTCTGTTCCCACGCTGTCGCATGTTCGGATTGTCTCCTTTTTGGGCTTCTTGTTTGTTGTAGATAGTCTCTTTTTGTATACTTCTGTCAGCACCATCATTACGACTCAGAAGCCTTCGGTTTCCCTCTTTTTTGCATTCGAGTACATGATCCTAGCAACAACAACACTGTCAACATTTGTGAAATATGTTTTCTATGTTAGTGACATGATTATGGAGGGACAATGGGAGAGGAAGCATGTCTACACTTTCTATTTGGAACTTATTCGGGACTTGCTCCACTTATCTATGTATTTGTGCTTCTTCATCGTGATTTTAATTCGGTACTATGGCTTCCCAGTGCACTTGATTCTCAAACTGTATGAGACGTTCAGGAACTTTAAAATTAGTGTTGCTGATTACCTCCGTTATCGAAAGATCACTTCAAACATGAATGACCGGTTCCCAGATGCTACACCTGAAGAGCTTACTGCAGGTGACGCAACCTGCATCATTTGTCGTGAAGAGATGACAACAGCCAAAAAACTATTGTGTGGACACCTTTTACATGTGGCTTGCTTGCGATCATGGTTAGAGCGACAACATACCTGCCCTATGTGCCGAGCGCTTGTTGTACCTCCCGAAGGTCGGACAGGTACAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000153 GO:0000209 GO:0000835 GO:0000836 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0023052 GO:0030163 GO:0030433 GO:0030968 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034620 GO:0034645 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070085 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071944 GO:0098796 GO:0098827 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1902494 GO:1990234 GO:1990381
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 map04120 map04141
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

312

Amino Acids

36.34

Weight (kDa)

8.55

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_SYVN1_N PF25563 1 - 241 4.2e-90 E3 ubiquitin-protein ligase synoviolin-like, TPR repeats
zf-rbx1 PF12678 259 - 299 9e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 260 - 299 3.7e-10 Ring finger domain
zf-C3HC4 PF00097 261 - 298 2.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 261 - 296 5.6e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 758
AasI GACNNNNNNGTC 1 cut(s) 295
Acc36I ACCTGC 3 cut(s) 758, 788, 890
AccI GTMKAC 2 cut(s) 374, 537
AclWI GGATC 1 cut(s) 439
AcuI CTGAAG 1 cut(s) 774
AfaI GTAC 5 cut(s) 281, 440, 620, 909, 931
AfeI AGCGCT 1 cut(s) 900
AfiI CCNNNNNNNGG 3 cut(s) 118, 917, 921
AflIII ACRYGT 1 cut(s) 841
AgeI ACCGGT 1 cut(s) 732
AgsI TTSAA 1 cut(s) 720
AluBI AGCT 3 cut(s) 94, 760, 935
AluI AGCT 3 cut(s) 94, 760, 935
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 4 cut(s) 281, 334, 368, 653
Alw44I GTGCAC 1 cut(s) 635
AlwI GGATC 1 cut(s) 439
Aor51HI AGCGCT 1 cut(s) 900
AoxI GGCC 1 cut(s) 52
ApaLI GTGCAC 1 cut(s) 635
AsiGI ACCGGT 1 cut(s) 732
Asp700I GAANNNNTTC 2 cut(s) 95, 558
AspLEI GCGC 2 cut(s) 265, 901
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 782
BaeGI GKGCMC 1 cut(s) 639
BarI GAAGNNNNNNTAC 6 cut(s) 521, 553, 578, 610, 611, 643
Bbv12I GWGCWC 1 cut(s) 639
BccI CCATC 1 cut(s) 398
BclI TGATCA 1 cut(s) 240
BcoDI GTCTC 4 cut(s) 281, 334, 368, 653
BfaI CTAG 1 cut(s) 449
BfmI CTRYAG 1 cut(s) 765
BfoI RGCGCY 1 cut(s) 902
BfuAI ACCTGC 3 cut(s) 758, 788, 890
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 737
BmrI ACTGGG 1 cut(s) 626
BmsI GCATC 2 cut(s) 733, 792
BmuI ACTGGG 1 cut(s) 626
BsaJI CCNNGG 1 cut(s) 100
BsaWI WCCGGW 1 cut(s) 732
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 917, 921
Bse118I RCCGGY 1 cut(s) 732
Bse1I ACTGG 1 cut(s) 632
BseDI CCNNGG 1 cut(s) 100
BseLI CCNNNNNNNGG 3 cut(s) 118, 917, 921
BseMII CTCAG 1 cut(s) 416
BseNI ACTGG 1 cut(s) 632
BseSI GKGCMC 1 cut(s) 639
BshFI GGCC 1 cut(s) 54
BshTI ACCGGT 1 cut(s) 732
BsiHKAI GWGCWC 1 cut(s) 639
BsiSI CCGG 3 cut(s) 119, 190, 733
BslFI GGGAC 2 cut(s) 528, 578
BslI CCNNNNNNNGG 3 cut(s) 118, 917, 921
BsmAI GTCTC 4 cut(s) 281, 334, 368, 653
BsmBI CGTCTC 1 cut(s) 653
BsmFI GGGAC 2 cut(s) 528, 578
BsmI GAATGC 1 cut(s) 431
BsnI GGCC 1 cut(s) 54
Bsp1286I GDGCHC 1 cut(s) 639
Bsp143I GATC 5 cut(s) 24, 240, 444, 711, 857
BspANI GGCC 1 cut(s) 54
BspCNI CTCAG 1 cut(s) 415
BspLI GGNNCC 1 cut(s) 737
BspMAI CTGCAG 1 cut(s) 769
BspMI ACCTGC 3 cut(s) 758, 788, 890
BspPI GGATC 1 cut(s) 439
BspQI GCTCTTC 1 cut(s) 750
BsrFI RCCGGY 1 cut(s) 732
BsrI ACTGG 1 cut(s) 632
BssAI RCCGGY 1 cut(s) 732
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 5 cut(s) 24, 240, 444, 711, 857
BssNAI GTATAC 1 cut(s) 375
Bst1107I GTATAC 1 cut(s) 375
Bst4CI ACNGT 2 cut(s) 465, 654
Bst6I CTCTTC 3 cut(s) 263, 750, 792
BstC8I GCNNGC 2 cut(s) 850, 854
BstDEI CTNAG 2 cut(s) 87, 402
BstENI CCTNNNNNAGG 1 cut(s) 915
BstH2I RGCGCY 1 cut(s) 902
BstHHI GCGC 2 cut(s) 265, 901
BstKTI GATC 5 cut(s) 27, 243, 447, 714, 860
BstMAI GTCTC 4 cut(s) 281, 334, 368, 653
BstMBI GATC 5 cut(s) 24, 240, 444, 711, 857
BstMWI GCNNNNNNNGC 3 cut(s) 139, 773, 891
BstNSI RCATGY 3 cut(s) 320, 536, 845
BstSFI CTRYAG 1 cut(s) 765
BstSLI GKGCMC 1 cut(s) 639
BstZ17I GTATAC 1 cut(s) 375
BsuRI GGCC 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 3 cut(s) 225, 461, 639
BveI ACCTGC 3 cut(s) 758, 788, 890
Cac8I GCNNGC 2 cut(s) 850, 854
CfoI GCGC 2 cut(s) 265, 901
Cfr10I RCCGGY 1 cut(s) 732
Cfr13I GGNCC 1 cut(s) 52
CseI GACGC 1 cut(s) 782
Csp6I GTAC 5 cut(s) 280, 439, 619, 908, 930
CspAI ACCGGT 1 cut(s) 732
CviQI GTAC 5 cut(s) 280, 439, 619, 908, 930
DdeI CTNAG 2 cut(s) 87, 402
DpnI GATC 5 cut(s) 26, 242, 446, 713, 859
DpnII GATC 5 cut(s) 24, 240, 444, 711, 857
DraI TTTAAA 1 cut(s) 676
DrdI GACNNNNNNGTC 1 cut(s) 295
DseDI GACNNNNNNGTC 1 cut(s) 295
Eam1104I CTCTTC 3 cut(s) 263, 750, 792
EarI CTCTTC 3 cut(s) 263, 750, 792
Eco47III AGCGCT 1 cut(s) 900
Eco57I CTGAAG 1 cut(s) 774
EcoNI CCTNNNNNAGG 1 cut(s) 915
Esp3I CGTCTC 1 cut(s) 653
FaqI GGGAC 2 cut(s) 528, 578
FbaI TGATCA 1 cut(s) 240
FblI GTMKAC 2 cut(s) 374, 537
FspBI CTAG 1 cut(s) 449
GlaI GCGC 2 cut(s) 264, 900
HaeII RGCGCY 1 cut(s) 902
HaeIII GGCC 1 cut(s) 54
HapII CCGG 3 cut(s) 119, 190, 733
HgaI GACGC 1 cut(s) 782
HhaI GCGC 2 cut(s) 265, 901
Hin6I GCGC 2 cut(s) 263, 899
HinP1I GCGC 2 cut(s) 263, 899
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HinfI GANTC 3 cut(s) 166, 400, 643
HpaII CCGG 3 cut(s) 119, 190, 733
HphI GGTGA 1 cut(s) 782
Hpy166II GTNNAC 6 cut(s) 7, 375, 468, 538, 637, 830
Hpy188I TCNGA 4 cut(s) 113, 324, 405, 924
Hpy188III TCNNGA 5 cut(s) 563, 604, 667, 794, 914
Hpy8I GTNNAC 6 cut(s) 7, 375, 468, 538, 637, 830
HpyAV CCTTC 2 cut(s) 420, 911
HpyCH4III ACNGT 2 cut(s) 465, 654
HpyCH4IV ACGT 1 cut(s) 662
HpyCH4V TGCA 5 cut(s) 253, 431, 637, 767, 783
HpyF10VI GCNNNNNNNGC 3 cut(s) 139, 773, 891
HpyF3I CTNAG 2 cut(s) 87, 402
HpySE526I ACGT 1 cut(s) 662
HspAI GCGC 2 cut(s) 263, 899
Ksp22I TGATCA 1 cut(s) 240
Kzo9I GATC 5 cut(s) 24, 240, 444, 711, 857
LguI GCTCTTC 1 cut(s) 750
LmnI GCTCC 1 cut(s) 576
LweI GCATC 2 cut(s) 733, 792
MaeI CTAG 1 cut(s) 449
MaeII ACGT 1 cut(s) 662
MaeIII GTNAC 3 cut(s) 223, 497, 770
MalI GATC 5 cut(s) 26, 242, 446, 713, 859
MboI GATC 5 cut(s) 24, 240, 444, 711, 857
MboII GAAGA 5 cut(s) 32, 280, 589, 767, 809
MhlI GDGCHC 1 cut(s) 639
MluCI AATT 2 cut(s) 612, 678
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 902
MroXI GAANNNNTTC 2 cut(s) 95, 558
MseI TTAA 3 cut(s) 135, 611, 675
MslI CAYNNNNRTG 1 cut(s) 506
MspI CCGG 3 cut(s) 119, 190, 733
Mva1269I GAATGC 1 cut(s) 431
MwoI GCNNNNNNNGC 3 cut(s) 139, 773, 891
NdeII GATC 5 cut(s) 24, 240, 444, 711, 857
NlaIV GGNNCC 1 cut(s) 737
NmeAIII GCCGAG 2 cut(s) 81, 920
NmuCI GTSAC 3 cut(s) 223, 497, 770
NspI RCATGY 3 cut(s) 320, 536, 845
PaqCI CACCTGC 1 cut(s) 758
PciI ACATGT 1 cut(s) 841
PciSI GCTCTTC 1 cut(s) 750
PctI GAATGC 1 cut(s) 431
PdmI GAANNNNTTC 2 cut(s) 95, 558
PfeI GAWTC 2 cut(s) 166, 643
PinAI ACCGGT 1 cut(s) 732
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
PscI ACATGT 1 cut(s) 841
PspN4I GGNNCC 1 cut(s) 737
PspPI GGNCC 1 cut(s) 52
PsrI GAACNNNNNNTAC 2 cut(s) 647, 679
PstI CTGCAG 1 cut(s) 769
RsaI GTAC 5 cut(s) 281, 440, 620, 909, 931
RsaNI GTAC 5 cut(s) 280, 439, 619, 908, 930
RseI CAYNNNNRTG 1 cut(s) 506
SapI GCTCTTC 1 cut(s) 750
SaqAI TTAA 3 cut(s) 135, 611, 675
Sau3AI GATC 5 cut(s) 24, 240, 444, 711, 857
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 1 cut(s) 394
SduI GDGCHC 1 cut(s) 639
SfaNI GCATC 2 cut(s) 733, 792
SfcI CTRYAG 1 cut(s) 765
SmiMI CAYNNNNRTG 1 cut(s) 506
Sse9I AATT 2 cut(s) 612, 678
SspMI CTAG 1 cut(s) 449
TaaI ACNGT 2 cut(s) 465, 654
TaiI ACGT 1 cut(s) 665
TaqI TCGA 3 cut(s) 14, 435, 706
TasI AATT 2 cut(s) 612, 678
TatI WGTACW 2 cut(s) 279, 438
TfiI GAWTC 2 cut(s) 166, 643
Tru1I TTAA 3 cut(s) 135, 611, 675
Tru9I TTAA 3 cut(s) 135, 611, 675
TscAI CASTG 3 cut(s) 232, 468, 639
TseFI GTSAC 3 cut(s) 223, 497, 770
Tsp45I GTSAC 3 cut(s) 223, 497, 770
TspDTI ATGAA 2 cut(s) 589, 740
TspGWI ACGGA 2 cut(s) 285, 689
TspRI CASTG 3 cut(s) 232, 468, 639
VneI GTGCAC 1 cut(s) 635
XagI CCTNNNNNAGG 1 cut(s) 915
XceI RCATGY 3 cut(s) 320, 536, 845
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
XmiI GTMKAC 2 cut(s) 374, 537
XmnI GAANNNNTTC 2 cut(s) 95, 558
XspI CTAG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.