Rh1AG469900

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003784
Physical Location & Seq
Reverse (-)
68293878 .. 68298175
4298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG469900.1

Sequence Viewer

Length: 1302 bp
ATGATGAGGCTGCAAACTTACGCGGGGCTTAGCTTGATAGGAACCCTAGCGATTATTTACCATGCATTTAGCAGCAGAGGCCAGTTTTACCCCGCCATGGTTTACCTATCAACCTCCAAGATCAGTCTGGTGCTTCTTCTCAACATGGGCCTTGTCATCATGTGTATTTTGTGGCAACTGACTAAGAAGCTATTCCTTGGCTCACTCCGAGAGCCGGAGGTTGAGAGGCTTAATGAGCAGTCGTGGAGGGAAGTCATGGAGATTCTTTTCGCAATCACTATTTTCCGGCAGGACTTTTCGGTGTCGTTTCTGGCTTTTGTCACTGCTCTGTTGCTGATCAAGGCGCTGCATTGGTTGGCGCAGAAGAGGGTTGAGTACATTGAGACTACTCCGTCTGTTCCCACGCTGTCGCATGTTCGGATTGTCTCCTTTTTGGGGTTCTTGTTTGTTGTAGATAGTCTCTTTTTGTATACTTCTGTCAGCACCATCATTACGACTCAGAAGCCTTCGGTTTCCCTCTTTTTTGCATTCGAGTACATGATCCTAGCAACAACAACACTGTCAACATTTGTGAAATATGTTTTCTATGTTAGTGACATGATTATGGAGGGACAATGGGAGAGGAAGCATGTCTACACTTTCTATTTGGAACTTATTCGGGACTTGCTCCACTTATCTATGTATTTGTGCTTCTTCATCGTGATTTTCATGTACTATGGCTTGCCAGTGCACTTGATTCGCGAACTGTATGAGACGTTCAGGAACTTTAAGATTCGTGTTGCTGATTACCTCCGTTATCGAAAGATCACTTCAAACATGAATGACCGGTTCCCAGATGCTACACCTGAAGAGCTTACTGCAGGTGACGCAACCTGCATCATTTGTCGTGAAGAGATGACAACAGCCAAAAAACTATTATGTGGACACCTTTTTCATGTGGCTTGCTTGCGATCATGGTTAGAGCGACAACATACCTGCCCTACGTGCCGAGCGCTTGTTGTACCTCCTGATGGTCGGACAGGTACAGCTCGAGGACAAGGTGGATCACGCTCCGATGCTCATCAACAGGGGACAGGCATATCAGGTACAACTGCTCAAGGTTCAACTAGTGGTGGAGGTGCTGGTGATAACATGAGCCATCATGAAGCAAGACTGCGAGCTGCAGCTGCAGCTGCATCAATATATGAGAAATCTTATGTATATCCATCTGCAAACAGACTAGTATGGTATGCAACTGTTATTGTAGTAAAGTGGTTACACTTTTATTTTTATCTGTTCATGGTCATTTCCTTTCCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000153 GO:0000209 GO:0000835 GO:0000836 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0023052 GO:0030163 GO:0030433 GO:0030968 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034620 GO:0034645 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070085 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071944 GO:0098796 GO:0098827 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1902494 GO:1990234 GO:1990381
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 map04120 map04141
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

433

Amino Acids

49.5

Weight (kDa)

8.97

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_SYVN1_N PF25563 3 - 272 7.4e-107 E3 ubiquitin-protein ligase synoviolin-like, TPR repeats
zf-rbx1 PF12678 290 - 330 1.1e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 291 - 330 5.9e-11 Ring finger domain
zf-C3HC4 PF00097 292 - 329 5.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 292 - 327 9.7e-07 RING-type zinc-finger
zf-C3HC4_2 PF13923 292 - 329 2.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 851
AasI GACNNNNNNGTC 1 cut(s) 391
Acc36I ACCTGC 3 cut(s) 851, 881, 983
AccI GTMKAC 2 cut(s) 470, 633
AccII CGCG 2 cut(s) 23, 741
AciI CCGC 2 cut(s) 23, 93
AclWI GGATC 2 cut(s) 535, 1051
AcuI CTGAAG 1 cut(s) 867
AfaI GTAC 6 cut(s) 377, 536, 713, 1002, 1024, 1087
AfeI AGCGCT 1 cut(s) 993
AfiI CCNNNNNNNGG 4 cut(s) 97, 214, 435, 1010
AgeI ACCGGT 1 cut(s) 825
AgsI TTSAA 2 cut(s) 813, 1104
AhlI ACTAGT 2 cut(s) 1106, 1219
AluBI AGCT 7 cut(s) 33, 190, 853, 1028, 1160, 1166, 1172
AluI AGCT 7 cut(s) 33, 190, 853, 1028, 1160, 1166, 1172
Alw21I GWGCWC 1 cut(s) 732
Alw26I GTCTC 4 cut(s) 377, 430, 464, 746
Alw44I GTGCAC 1 cut(s) 728
AlwI GGATC 2 cut(s) 535, 1051
Ama87I CYCGRG 1 cut(s) 1029
Aor51HI AGCGCT 1 cut(s) 993
AoxI GGCC 2 cut(s) 79, 148
ApaLI GTGCAC 1 cut(s) 728
ApeKI GCWGC 8 cut(s) 10, 72, 346, 1160, 1163, 1166, 1169, 1172
AsiGI ACCGGT 1 cut(s) 825
Asp700I GAANNNNTTC 2 cut(s) 191, 654
AspLEI GCGC 3 cut(s) 346, 361, 994
AspS9I GGNCC 1 cut(s) 148
AsuHPI GGTGA 2 cut(s) 875, 1136
AvaI CYCGRG 1 cut(s) 1029
BaeGI GKGCMC 1 cut(s) 732
BarI GAAGNNNNNNTAC 4 cut(s) 617, 649, 674, 706
Bbv12I GWGCWC 1 cut(s) 732
BbvI GCAGC 7 cut(s) 84, 333, 1147, 1153, 1159, 1175, 1181
BccI CCATC 4 cut(s) 494, 1004, 1146, 1213
BcgI CGANNNNNNTGC 2 cut(s) 719, 753
BclI TGATCA 1 cut(s) 336
BcoDI GTCTC 4 cut(s) 377, 430, 464, 746
BcuI ACTAGT 2 cut(s) 1106, 1219
BfaI CTAG 4 cut(s) 47, 545, 1107, 1220
BfmI CTRYAG 3 cut(s) 858, 1161, 1167
BfoI RGCGCY 2 cut(s) 347, 995
BfuAI ACCTGC 3 cut(s) 851, 881, 983
BisI GCNGC 8 cut(s) 11, 73, 347, 1161, 1164, 1167, 1170, 1173
BlpI GCTNAGC 1 cut(s) 29
BlsI GCNGC 8 cut(s) 12, 74, 348, 1162, 1165, 1168, 1171, 1174
BmeT110I CYCGRG 1 cut(s) 1029
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 2 cut(s) 43, 830
BmsI GCATC 4 cut(s) 826, 885, 1045, 1184
Bpu1102I GCTNAGC 1 cut(s) 29
BpuEI CTTGAG 1 cut(s) 1080
BsaAI YACGTR 1 cut(s) 984
BsaBI GATNNNNATC 1 cut(s) 1059
BsaJI CCNNGG 2 cut(s) 96, 196
BsaWI WCCGGW 1 cut(s) 825
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 214, 435, 1010
Bse118I RCCGGY 1 cut(s) 825
Bse1I ACTGG 2 cut(s) 82, 725
Bse8I GATNNNNATC 1 cut(s) 1059
BseDI CCNNGG 2 cut(s) 96, 196
BseJI GATNNNNATC 1 cut(s) 1059
BseLI CCNNNNNNNGG 4 cut(s) 97, 214, 435, 1010
BseMII CTCAG 1 cut(s) 512
BseNI ACTGG 2 cut(s) 82, 725
BseSI GKGCMC 1 cut(s) 732
BseXI GCAGC 7 cut(s) 84, 333, 1147, 1153, 1159, 1175, 1181
Bsh1236I CGCG 2 cut(s) 23, 741
BshFI GGCC 2 cut(s) 81, 150
BshTI ACCGGT 1 cut(s) 825
BsiHKAI GWGCWC 1 cut(s) 732
BsiHKCI CYCGRG 1 cut(s) 1029
BsiSI CCGG 3 cut(s) 215, 286, 826
BslFI GGGAC 3 cut(s) 624, 674, 1084
BslI CCNNNNNNNGG 4 cut(s) 97, 214, 435, 1010
BsmAI GTCTC 4 cut(s) 377, 430, 464, 746
BsmBI CGTCTC 1 cut(s) 746
BsmFI GGGAC 3 cut(s) 624, 674, 1084
BsmI GAATGC 1 cut(s) 527
BsnI GGCC 2 cut(s) 81, 150
BsoBI CYCGRG 1 cut(s) 1029
Bsp1286I GDGCHC 1 cut(s) 732
Bsp143I GATC 6 cut(s) 120, 336, 540, 804, 950, 1043
Bsp1720I GCTNAGC 1 cut(s) 29
Bsp19I CCATGG 1 cut(s) 96
Bsp68I TCGCGA 1 cut(s) 741
BspACI CCGC 2 cut(s) 23, 93
BspANI GGCC 2 cut(s) 81, 150
BspCNI CTCAG 1 cut(s) 511
BspFNI CGCG 2 cut(s) 23, 741
BspHI TCATGA 1 cut(s) 1141
BspLI GGNNCC 2 cut(s) 43, 830
BspMAI CTGCAG 3 cut(s) 862, 1165, 1171
BspMI ACCTGC 3 cut(s) 851, 881, 983
BspPI GGATC 2 cut(s) 535, 1051
BspQI GCTCTTC 1 cut(s) 843
BsrFI RCCGGY 1 cut(s) 825
BsrI ACTGG 2 cut(s) 82, 725
BssAI RCCGGY 1 cut(s) 825
BssECI CCNNGG 2 cut(s) 96, 196
BssMI GATC 6 cut(s) 120, 336, 540, 804, 950, 1043
BssNAI GTATAC 1 cut(s) 471
BssT1I CCWWGG 2 cut(s) 96, 196
Bst1107I GTATAC 1 cut(s) 471
Bst4CI ACNGT 3 cut(s) 561, 747, 1237
Bst6I CTCTTC 3 cut(s) 359, 843, 885
BstBAI YACGTR 1 cut(s) 984
BstC8I GCNNGC 4 cut(s) 722, 943, 947, 1158
BstDEI CTNAG 3 cut(s) 29, 183, 498
BstDSI CCRYGG 1 cut(s) 96
BstFNI CGCG 2 cut(s) 23, 741
BstH2I RGCGCY 2 cut(s) 347, 995
BstHHI GCGC 3 cut(s) 346, 361, 994
BstKTI GATC 6 cut(s) 123, 339, 543, 807, 953, 1046
BstMAI GTCTC 4 cut(s) 377, 430, 464, 746
BstMBI GATC 6 cut(s) 120, 336, 540, 804, 950, 1043
BstMWI GCNNNNNNNGC 7 cut(s) 78, 235, 866, 984, 1166, 1169, 1172
BstNSI RCATGY 2 cut(s) 416, 632
BstSFI CTRYAG 3 cut(s) 858, 1161, 1167
BstSLI GKGCMC 1 cut(s) 732
BstUI CGCG 2 cut(s) 23, 741
BstV1I GCAGC 7 cut(s) 84, 333, 1147, 1153, 1159, 1175, 1181
BstZ17I GTATAC 1 cut(s) 471
BsuRI GGCC 2 cut(s) 81, 150
BtgI CCRYGG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 3 cut(s) 321, 557, 732
BtuMI TCGCGA 1 cut(s) 741
BveI ACCTGC 3 cut(s) 851, 881, 983
Cac8I GCNNGC 4 cut(s) 722, 943, 947, 1158
CciI TCATGA 1 cut(s) 1141
CfoI GCGC 3 cut(s) 346, 361, 994
Cfr10I RCCGGY 1 cut(s) 825
Cfr13I GGNCC 1 cut(s) 148
CseI GACGC 1 cut(s) 875
Csp6I GTAC 6 cut(s) 376, 535, 712, 1001, 1023, 1086
CspAI ACCGGT 1 cut(s) 825
CviQI GTAC 6 cut(s) 376, 535, 712, 1001, 1023, 1086
DdeI CTNAG 3 cut(s) 29, 183, 498
DpnI GATC 6 cut(s) 122, 338, 542, 806, 952, 1045
DpnII GATC 6 cut(s) 120, 336, 540, 804, 950, 1043
DrdI GACNNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 1 cut(s) 391
Eam1104I CTCTTC 3 cut(s) 359, 843, 885
EarI CTCTTC 3 cut(s) 359, 843, 885
Eco130I CCWWGG 2 cut(s) 96, 196
Eco47III AGCGCT 1 cut(s) 993
Eco57I CTGAAG 1 cut(s) 867
Eco88I CYCGRG 1 cut(s) 1029
EcoT14I CCWWGG 2 cut(s) 96, 196
EcoT22I ATGCAT 1 cut(s) 67
ErhI CCWWGG 2 cut(s) 96, 196
Esp3I CGTCTC 1 cut(s) 746
FaqI GGGAC 3 cut(s) 624, 674, 1084
FauI CCCGC 2 cut(s) 16, 100
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 2 cut(s) 470, 633
Fnu4HI GCNGC 8 cut(s) 11, 73, 347, 1161, 1164, 1167, 1170, 1173
Fsp4HI GCNGC 8 cut(s) 11, 73, 347, 1161, 1164, 1167, 1170, 1173
FspBI CTAG 4 cut(s) 47, 545, 1107, 1220
GlaI GCGC 3 cut(s) 345, 360, 993
GluI GCNGC 8 cut(s) 11, 73, 347, 1161, 1164, 1167, 1170, 1173
HaeII RGCGCY 2 cut(s) 347, 995
HaeIII GGCC 2 cut(s) 81, 150
HapII CCGG 3 cut(s) 215, 286, 826
HgaI GACGC 1 cut(s) 875
HhaI GCGC 3 cut(s) 346, 361, 994
Hin6I GCGC 3 cut(s) 344, 359, 992
HinP1I GCGC 3 cut(s) 344, 359, 992
HincII GTYRAC 1 cut(s) 564
HindII GTYRAC 1 cut(s) 564
HinfI GANTC 4 cut(s) 262, 496, 736, 772
HpaII CCGG 3 cut(s) 215, 286, 826
HphI GGTGA 2 cut(s) 875, 1136
Hpy166II GTNNAC 6 cut(s) 103, 471, 564, 634, 730, 923
Hpy188I TCNGA 5 cut(s) 209, 420, 501, 1017, 1054
Hpy188III TCNNGA 7 cut(s) 659, 700, 740, 760, 887, 1007, 1142
Hpy8I GTNNAC 6 cut(s) 103, 471, 564, 634, 730, 923
HpyAV CCTTC 1 cut(s) 516
HpyCH4III ACNGT 3 cut(s) 561, 747, 1237
HpyCH4IV ACGT 2 cut(s) 755, 983
HpyF10VI GCNNNNNNNGC 7 cut(s) 78, 235, 866, 984, 1166, 1169, 1172
HpyF3I CTNAG 3 cut(s) 29, 183, 498
HpySE526I ACGT 2 cut(s) 755, 983
HspAI GCGC 3 cut(s) 344, 359, 992
Ksp22I TGATCA 1 cut(s) 336
Kzo9I GATC 6 cut(s) 120, 336, 540, 804, 950, 1043
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 2 cut(s) 672, 1055
Lsp1109I GCAGC 7 cut(s) 84, 333, 1147, 1153, 1159, 1175, 1181
LweI GCATC 4 cut(s) 826, 885, 1045, 1184
MaeI CTAG 4 cut(s) 47, 545, 1107, 1220
MaeII ACGT 2 cut(s) 755, 983
MaeIII GTNAC 4 cut(s) 319, 593, 863, 1254
MalI GATC 6 cut(s) 122, 338, 542, 806, 952, 1045
MboI GATC 6 cut(s) 120, 336, 540, 804, 950, 1043
MboII GAAGA 5 cut(s) 128, 376, 685, 860, 902
MhlI GDGCHC 1 cut(s) 732
MlyI GAGTC 1 cut(s) 490
MmeI TCCRAC 1 cut(s) 995
Mph1103I ATGCAT 1 cut(s) 67
MroXI GAANNNNTTC 2 cut(s) 191, 654
MseI TTAA 2 cut(s) 231, 768
MslI CAYNNNNRTG 1 cut(s) 602
MspA1I CMGCKG 2 cut(s) 1166, 1172
MspI CCGG 3 cut(s) 215, 286, 826
Mva1269I GAATGC 1 cut(s) 527
MvnI CGCG 2 cut(s) 23, 741
MwoI GCNNNNNNNGC 7 cut(s) 78, 235, 866, 984, 1166, 1169, 1172
NcoI CCATGG 1 cut(s) 96
NdeII GATC 6 cut(s) 120, 336, 540, 804, 950, 1043
NlaIV GGNNCC 2 cut(s) 43, 830
NmeAIII GCCGAG 1 cut(s) 1013
NmuCI GTSAC 3 cut(s) 319, 593, 863
NruI TCGCGA 1 cut(s) 741
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 2 cut(s) 416, 632
PaeR7I CTCGAG 1 cut(s) 1029
PagI TCATGA 1 cut(s) 1141
PaqCI CACCTGC 1 cut(s) 851
PciSI GCTCTTC 1 cut(s) 843
PctI GAATGC 1 cut(s) 527
PdmI GAANNNNTTC 2 cut(s) 191, 654
PfeI GAWTC 3 cut(s) 262, 736, 772
PinAI ACCGGT 1 cut(s) 825
PkrI GCNGC 8 cut(s) 12, 74, 348, 1162, 1165, 1168, 1171, 1174
PleI GAGTC 1 cut(s) 490
PpsI GAGTC 1 cut(s) 490
Ppu21I YACGTR 1 cut(s) 984
PspN4I GGNNCC 2 cut(s) 43, 830
PspPI GGNCC 1 cut(s) 148
PspXI VCTCGAGB 1 cut(s) 1029
PsrI GAACNNNNNNTAC 2 cut(s) 740, 772
PstI CTGCAG 3 cut(s) 862, 1165, 1171
PvuII CAGCTG 2 cut(s) 1166, 1172
RruI TCGCGA 1 cut(s) 741
RsaI GTAC 6 cut(s) 377, 536, 713, 1002, 1024, 1087
RsaNI GTAC 6 cut(s) 376, 535, 712, 1001, 1023, 1086
RseI CAYNNNNRTG 1 cut(s) 602
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 2 cut(s) 231, 768
SatI GCNGC 8 cut(s) 11, 73, 347, 1161, 1164, 1167, 1170, 1173
Sau3AI GATC 6 cut(s) 120, 336, 540, 804, 950, 1043
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 1 cut(s) 490
SduI GDGCHC 1 cut(s) 732
SfaNI GCATC 4 cut(s) 826, 885, 1045, 1184
SfcI CTRYAG 3 cut(s) 858, 1161, 1167
Sfr274I CTCGAG 1 cut(s) 1029
SlaI CTCGAG 1 cut(s) 1029
SmiMI CAYNNNNRTG 1 cut(s) 602
SmlI CTYRAG 2 cut(s) 1029, 1095
SmoI CTYRAG 2 cut(s) 1029, 1095
SpeI ACTAGT 2 cut(s) 1106, 1219
SsiI CCGC 2 cut(s) 23, 93
SspMI CTAG 4 cut(s) 47, 545, 1107, 1220
StyI CCWWGG 2 cut(s) 96, 196
TaaI ACNGT 3 cut(s) 561, 747, 1237
TaiI ACGT 2 cut(s) 758, 986
TaqI TCGA 3 cut(s) 531, 799, 1030
TatI WGTACW 3 cut(s) 375, 534, 711
TfiI GAWTC 3 cut(s) 262, 736, 772
Tru1I TTAA 2 cut(s) 231, 768
Tru9I TTAA 2 cut(s) 231, 768
TscAI CASTG 3 cut(s) 328, 564, 732
TseFI GTSAC 3 cut(s) 319, 593, 863
TseI GCWGC 8 cut(s) 10, 72, 346, 1160, 1163, 1166, 1169, 1172
Tsp45I GTSAC 3 cut(s) 319, 593, 863
TspDTI ATGAA 6 cut(s) 685, 697, 833, 923, 1158, 1267
TspGWI ACGGA 2 cut(s) 381, 782
TspRI CASTG 3 cut(s) 328, 564, 732
VneI GTGCAC 1 cut(s) 728
XceI RCATGY 2 cut(s) 416, 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XhoI CTCGAG 1 cut(s) 1029
XmiI GTMKAC 2 cut(s) 470, 633
XmnI GAANNNNTTC 2 cut(s) 191, 654
XspI CTAG 4 cut(s) 47, 545, 1107, 1220
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.