Prupe.6G242100_v2.0.a1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0003784
Physical Location & Seq
Reverse (-)
24051999 .. 24053093
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G242100.1

Sequence Viewer

Length: 570 bp
ACAGGCACAACAGCTCAAGGTGGTGGGGTTGCTGGTGATAATCTCAGTCAACATGAAGCCAGACTCCAAGCTGCTGCTGCTGCAGCATCGATATATGAGAAGTCTCATGTATATCCATCTGCAAACAGACTAGTCTCCTGGACATGTGGCACAGTGGCACTTCCTCAGTTTCAAAGACAAATGACCGACTCCGCTAATACTGAATCTAGTGGAGTATCGCACCCACAGCTTGCAACTCCTGGTGGACCTTCAAACTTGTCCTCCCCTCAATTTCCACATTACATGTTTGTCCCATTTGAGGTTCCTGGAGCCAATGTGGCCTATGGGGAGAGGTTGGGCGGCGATCCGAATATAACAGCTTCTCAACTGGAGGCCCAGAAAAAATTTCTCCAACACCAGATCGAGATCCTGCAGAATCAGCTCCAAACTTTACAGAAGCCAAAACTGAAGGAAAGCATGGACACGGGTCCATCAGCATCTTCGGATGGCAAAGGAAAGAATGTCGAATCTTCGTCCTCATCTGTTTCAGATTGTGGACGTCATGTGGAGCTTGAAGAGATTGATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000153 GO:0000209 GO:0000835 GO:0000836 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0023052 GO:0030163 GO:0030433 GO:0030968 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034620 GO:0034645 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070085 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071944 GO:0098796 GO:0098827 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1902494 GO:1990234 GO:1990381
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 map04120 map04141
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

190

Amino Acids

20.0

Weight (kDa)

5.44

Isoelectric Point (pI)

56.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 541
AciI CCGC 2 cut(s) 192, 339
AclWI GGATC 2 cut(s) 338, 400
AcsI RAATTY 1 cut(s) 383
AcuI CTGAAG 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 538
AfiI CCNNNNNNNGG 1 cut(s) 298
AflIII ACRYGT 2 cut(s) 143, 282
AgsI TTSAA 3 cut(s) 173, 252, 554
AhlI ACTAGT 1 cut(s) 130
AjnI CCWGG 3 cut(s) 137, 238, 304
AluBI AGCT 6 cut(s) 14, 71, 229, 359, 421, 550
AluI AGCT 6 cut(s) 14, 71, 229, 359, 421, 550
Alw26I GTCTC 2 cut(s) 108, 139
AlwI GGATC 2 cut(s) 338, 400
AoxI GGCC 2 cut(s) 318, 372
ApeKI GCWGC 5 cut(s) 71, 74, 77, 80, 83
ApoI RAATTY 1 cut(s) 383
AspS9I GGNCC 3 cut(s) 245, 373, 467
AsuHPI GGTGA 1 cut(s) 47
AvaII GGWCC 2 cut(s) 245, 467
BbvI GCAGC 5 cut(s) 58, 61, 64, 67, 95
BccI CCATC 3 cut(s) 124, 478, 479
BciT130I CCWGG 3 cut(s) 139, 240, 306
BcoDI GTCTC 2 cut(s) 108, 139
BcuI ACTAGT 1 cut(s) 130
BfaI CTAG 2 cut(s) 131, 207
BfmI CTRYAG 2 cut(s) 81, 410
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 6 cut(s) 72, 75, 78, 81, 84, 340
BlsI GCNGC 6 cut(s) 73, 76, 79, 82, 85, 341
Bme1390I CCNGG 3 cut(s) 139, 240, 306
Bme18I GGWCC 2 cut(s) 245, 467
BmgT120I GGNCC 3 cut(s) 245, 373, 467
BmiI GGNNCC 3 cut(s) 303, 310, 468
BmrFI CCNGG 3 cut(s) 139, 240, 306
BmsI GCATC 2 cut(s) 95, 485
BoxI GACNNNNGTC 1 cut(s) 465
BpmI CTGGAG 2 cut(s) 327, 389
Bsa29I ATCGAT 1 cut(s) 89
BsaBI GATNNNNATC 1 cut(s) 404
BsaHI GRCGYC 1 cut(s) 538
BsaXI ACNNNNNCTCC 4 cut(s) 245, 275, 300, 330
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse1I ACTGG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 404
BseBI CCWGG 3 cut(s) 139, 240, 306
BseCI ATCGAT 1 cut(s) 89
BseGI GGATG 1 cut(s) 490
BseJI GATNNNNATC 1 cut(s) 404
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMII CTCAG 2 cut(s) 58, 179
BseNI ACTGG 1 cut(s) 372
BseXI GCAGC 5 cut(s) 58, 61, 64, 67, 95
BshFI GGCC 2 cut(s) 320, 374
BshVI ATCGAT 1 cut(s) 89
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 2 cut(s) 108, 139
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 2 cut(s) 320, 374
Bsp143I GATC 3 cut(s) 343, 399, 405
BspACI CCGC 2 cut(s) 192, 339
BspANI GGCC 2 cut(s) 320, 374
BspCNI CTCAG 2 cut(s) 57, 178
BspDI ATCGAT 1 cut(s) 89
BspLI GGNNCC 3 cut(s) 303, 310, 468
BspMAI CTGCAG 2 cut(s) 85, 414
BspPI GGATC 2 cut(s) 338, 400
BsrI ACTGG 1 cut(s) 372
BssMI GATC 3 cut(s) 343, 399, 405
BssNI GRCGYC 1 cut(s) 538
Bst2UI CCWGG 3 cut(s) 139, 240, 306
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 1 cut(s) 549
BstACI GRCGYC 1 cut(s) 538
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 2 cut(s) 44, 165
BstF5I GGATG 1 cut(s) 490
BstKTI GATC 3 cut(s) 346, 402, 408
BstMAI GTCTC 2 cut(s) 108, 139
BstMBI GATC 3 cut(s) 343, 399, 405
BstMWI GCNNNNNNNGC 6 cut(s) 77, 80, 83, 226, 317, 418
BstNI CCWGG 3 cut(s) 139, 240, 306
BstNSI RCATGY 2 cut(s) 147, 286
BstPAI GACNNNNGTC 1 cut(s) 465
BstSCI CCNGG 3 cut(s) 137, 238, 304
BstSFI CTRYAG 2 cut(s) 81, 410
BstV1I GCAGC 5 cut(s) 58, 61, 64, 67, 95
BstX2I RGATCY 1 cut(s) 405
BstYI RGATCY 1 cut(s) 405
Bsu15I ATCGAT 1 cut(s) 89
BsuRI GGCC 2 cut(s) 320, 374
BsuTUI ATCGAT 1 cut(s) 89
BtsCI GGATG 1 cut(s) 490
BtsIMutI CAGTG 1 cut(s) 159
Cac8I GCNNGC 1 cut(s) 231
Cfr13I GGNCC 3 cut(s) 245, 373, 467
ClaI ATCGAT 1 cut(s) 89
CviAII CATG 6 cut(s) 53, 107, 144, 283, 457, 542
DdeI CTNAG 2 cut(s) 44, 165
DpnI GATC 3 cut(s) 345, 401, 407
DpnII GATC 3 cut(s) 343, 399, 405
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
Eco47I GGWCC 2 cut(s) 245, 467
Eco57I CTGAAG 1 cut(s) 467
EcoRII CCWGG 3 cut(s) 137, 238, 304
FaeI CATG 6 cut(s) 56, 110, 147, 286, 460, 545
FaqI GGGAC 1 cut(s) 275
FatI CATG 6 cut(s) 52, 106, 143, 282, 456, 541
Fnu4HI GCNGC 6 cut(s) 72, 75, 78, 81, 84, 340
FokI GGATG 1 cut(s) 497
Fsp4HI GCNGC 6 cut(s) 72, 75, 78, 81, 84, 340
FspBI CTAG 2 cut(s) 131, 207
GluI GCNGC 6 cut(s) 72, 75, 78, 81, 84, 340
GsuI CTGGAG 2 cut(s) 327, 389
HaeIII GGCC 2 cut(s) 320, 374
Hin1I GRCGYC 1 cut(s) 538
Hin1II CATG 6 cut(s) 56, 110, 147, 286, 460, 545
HincII GTYRAC 1 cut(s) 50
HindII GTYRAC 1 cut(s) 50
HinfI GANTC 6 cut(s) 63, 188, 203, 415, 506, 563
HphI GGTGA 1 cut(s) 47
Hpy166II GTNNAC 3 cut(s) 50, 245, 536
Hpy188I TCNGA 3 cut(s) 348, 484, 529
Hpy188III TCNNGA 1 cut(s) 403
Hpy8I GTNNAC 3 cut(s) 50, 245, 536
HpyAV CCTTC 2 cut(s) 258, 442
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 1 cut(s) 538
HpyCH4V TGCA 4 cut(s) 83, 122, 233, 412
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 80, 83, 226, 317, 418
HpyF3I CTNAG 2 cut(s) 44, 165
HpySE526I ACGT 1 cut(s) 538
Hsp92I GRCGYC 1 cut(s) 538
Hsp92II CATG 6 cut(s) 56, 110, 147, 286, 460, 545
Kzo9I GATC 3 cut(s) 343, 399, 405
LmnI GCTCC 3 cut(s) 308, 426, 547
Lsp1109I GCAGC 5 cut(s) 58, 61, 64, 67, 95
LweI GCATC 2 cut(s) 95, 485
MaeI CTAG 2 cut(s) 131, 207
MaeII ACGT 1 cut(s) 538
MalI GATC 3 cut(s) 345, 401, 407
MboI GATC 3 cut(s) 343, 399, 405
MboII GAAGA 3 cut(s) 471, 501, 566
MflI RGATCY 1 cut(s) 405
MluCI AATT 2 cut(s) 269, 383
MlyI GAGTC 2 cut(s) 57, 182
MmeI TCCRAC 1 cut(s) 415
MnlI CCTC 7 cut(s) 174, 271, 276, 292, 324, 364, 526
MspR9I CCNGG 3 cut(s) 139, 240, 306
MvaI CCWGG 3 cut(s) 139, 240, 306
MwoI GCNNNNNNNGC 6 cut(s) 77, 80, 83, 226, 317, 418
NdeII GATC 3 cut(s) 343, 399, 405
NlaIII CATG 6 cut(s) 56, 110, 147, 286, 460, 545
NlaIV GGNNCC 3 cut(s) 303, 310, 468
NspI RCATGY 2 cut(s) 147, 286
PciI ACATGT 2 cut(s) 143, 282
PfeI GAWTC 4 cut(s) 203, 415, 506, 563
PfoI TCCNGGA 2 cut(s) 137, 304
PkrI GCNGC 6 cut(s) 73, 76, 79, 82, 85, 341
PleI GAGTC 2 cut(s) 57, 182
PpsI GAGTC 2 cut(s) 57, 182
PscI ACATGT 2 cut(s) 143, 282
PshAI GACNNNNGTC 1 cut(s) 465
Psp6I CCWGG 3 cut(s) 137, 238, 304
PspGI CCWGG 3 cut(s) 137, 238, 304
PspN4I GGNNCC 3 cut(s) 303, 310, 468
PspPI GGNCC 3 cut(s) 245, 373, 467
PstI CTGCAG 2 cut(s) 85, 414
PsuI RGATCY 1 cut(s) 405
SatI GCNGC 6 cut(s) 72, 75, 78, 81, 84, 340
Sau3AI GATC 3 cut(s) 343, 399, 405
Sau96I GGNCC 3 cut(s) 245, 373, 467
SchI GAGTC 2 cut(s) 57, 182
ScrFI CCNGG 3 cut(s) 139, 240, 306
SfaNI GCATC 2 cut(s) 95, 485
SfcI CTRYAG 2 cut(s) 81, 410
SinI GGWCC 2 cut(s) 245, 467
SmlI CTYRAG 1 cut(s) 15
SmoI CTYRAG 1 cut(s) 15
SpeI ACTAGT 1 cut(s) 130
Sse9I AATT 2 cut(s) 269, 383
SsiI CCGC 2 cut(s) 192, 339
SspMI CTAG 2 cut(s) 131, 207
StyD4I CCNGG 3 cut(s) 137, 238, 304
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 1 cut(s) 541
TaqI TCGA 3 cut(s) 89, 402, 504
TaqII GACCGA 1 cut(s) 200
TasI AATT 2 cut(s) 269, 383
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 4 cut(s) 203, 415, 506, 563
TscAI CASTG 1 cut(s) 159
TseI GCWGC 5 cut(s) 71, 74, 77, 80, 83
TspDTI ATGAA 2 cut(s) 69, 555
TspRI CASTG 1 cut(s) 159
VpaK11BI GGWCC 2 cut(s) 245, 467
XapI RAATTY 1 cut(s) 383
XceI RCATGY 2 cut(s) 147, 286
XspI CTAG 2 cut(s) 131, 207
ZraI GACGTC 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.