Rh1BG427500

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0003784
Physical Location & Seq
Reverse (-)
54541868 .. 54546167
4300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG427500.1

Sequence Viewer

Length: 1614 bp
ATGATGAGGCTGCAAACTTACGCGGGGCTTAGCTTGATAGGAACCCTAGCGATTATTTACCATGCATTTAGCAGCAGAGGCCAGTTTTACCCCGCCATGGTTTACCTATCGACCTCCAAGATCAGTCTGGTGCTTCTTCTCAACATGGGCCTTGTCATCATGTGTATTTTGTGGCAACTGACTAAGAAGCTATTCCTCGGCTCACTCCGAGAGCCGGAGGTTGAGAGACTTAATGAGCAGTCGTGGAGGGAAGTCATGGAGATTCTTTTCGCAATCACTATTTTCCGGCAGGACTTTTCGGTGTCGTTTCTGGCTTTTGTCACTGCTCTGTTGCTGATCAAGGCGTTGCATTGGTTGGCGCAGAAGAGGGTTGAGTACATTGAGACTACTCCGTCTGTTCCCACGCTGTCCCATGTTCGGATTGTCTCCTTTTTGGGCTTCTTGTTTGTTGTAGATAGTCTCTTTTTGTATACTTCTGTCAGCACCATCATTACGACTCAGAAGCCTTCGGTTTCCCTCTTTTTTGCATTCGAGTACATGATCCTAGCAACAACAACACTGTCAACATTTGTGAAATATGTTTTCTATGTTAGTGACATGATTATGGAGGGACAATGGGAGAGGAAGCATGTCTACACTTTCTATTTGGAACTTATTCGGGACTTGCTCCACTTATCTATGTATTTGTGCTTCTTCATCGTGATTTTCATGTACTATGGCTTGCCAGTGCACTTGATTCGCGAACTGTATGAGACATTCAGGAACTTTAAGATTCGTGTTGCTGATTACCTCCGTTATCGAAAGATCACTTCAAACATGAATGACCGGTTCCCAGATGCTACACCTGAAGAGCTTACTGCAGGTGACGCAACCTGCATCATTTGTCGTGAAGAGATGACCACAGCCAAAAAACTATTGTGTGGACACCTTTTTCATGTGGCTTGCTTGCGATCATGGTTAGAGCGACAACATACCTGCCCTACGTGCCGAGCGCTTGTTGTACCTCCTGATGGTCGGACAGGTACAGCTCGAGGACAAGGTGGATCACGCTCCGATGCTCATCAACAGGGGACAGGCATATCAGGTACAACTGCTCAAGGTTCAACTAGTGGTGGAGGTGCTGGTGATAACATGAGCCATCATGAAGCAAGACTGCAAGCTGCTGCTGCAGCTGCATCAATATATGAGAAATCTTATGTATATCCATCTGCAAACAGACTAGTATGGTCTGCTGGATATGCCGCACTTCCTCTGGTTAATAGACATATGGCTGACTCCCCTAGTACTGATTTACAATCACAACCACTTGCAACCCCTGGTGGACCTGCAAACTTGTCATTGCCTCAACCTCCGCATTACATGTTTTCTCCTTTTCATGGAGCCAATGGGGAGAGGTCGGGCGGCAATACAAATATAACAGCTTCTCAGCTGGAGGCCCAGAGAAAAGTTCTTGAGCACCAGATTGAGATCCTGCAGAATCAGCTCCAAATTTTACAAAAGCCAAAACCCAAGGAAAGTGTGGAAGGGGTTCCAACATTAGCAGATAGCAAAGGAAAGAGTGTTGCACCTTTGTCTGAATCAAATTGCGGGTGTCATGAGGATGTTGTTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000153 GO:0000209 GO:0000835 GO:0000836 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0023052 GO:0030163 GO:0030433 GO:0030968 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034620 GO:0034645 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070085 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071944 GO:0098796 GO:0098827 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1902494 GO:1990234 GO:1990381
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 map04120 map04141
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

537

Amino Acids

60.11

Weight (kDa)

7.98

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_SYVN1_N PF25563 3 - 272 1.3e-106 E3 ubiquitin-protein ligase synoviolin-like, TPR repeats
zf-rbx1 PF12678 290 - 330 1.2e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 291 - 330 7.9e-11 Ring finger domain
zf-C3HC4 PF00097 292 - 329 7e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 292 - 327 1.3e-06 RING-type zinc-finger
zf-C3HC4_2 PF13923 292 - 329 3.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 851
AasI GACNNNNNNGTC 1 cut(s) 391
Acc36I ACCTGC 4 cut(s) 851, 881, 983, 1333
AccI GTMKAC 2 cut(s) 470, 633
AccII CGCG 2 cut(s) 23, 741
AciI CCGC 6 cut(s) 23, 93, 1242, 1352, 1401, 1587
AclWI GGATC 3 cut(s) 535, 1051, 1462
AcsI RAATTY 1 cut(s) 1488
AcuI CTGAAG 1 cut(s) 867
AfaI GTAC 7 cut(s) 377, 536, 713, 1002, 1024, 1087, 1285
AfeI AGCGCT 1 cut(s) 993
AfiI CCNNNNNNNGG 5 cut(s) 97, 214, 417, 1010, 1376
AflIII ACRYGT 1 cut(s) 1359
AgeI ACCGGT 1 cut(s) 825
AgsI TTSAA 2 cut(s) 813, 1104
AhlI ACTAGT 2 cut(s) 1106, 1219
AjnI CCWGG 1 cut(s) 1315
AluBI AGCT 9 cut(s) 33, 190, 853, 1028, 1160, 1172, 1421, 1429, 1483
AluI AGCT 9 cut(s) 33, 190, 853, 1028, 1160, 1172, 1421, 1429, 1483
Alw21I GWGCWC 2 cut(s) 732, 1458
Alw26I GTCTC 5 cut(s) 220, 377, 430, 464, 746
Alw44I GTGCAC 1 cut(s) 728
AlwI GGATC 3 cut(s) 535, 1051, 1462
Ama87I CYCGRG 1 cut(s) 1029
Aor51HI AGCGCT 1 cut(s) 993
AoxI GGCC 3 cut(s) 79, 148, 1434
ApaLI GTGCAC 1 cut(s) 728
ApeKI GCWGC 7 cut(s) 10, 72, 1160, 1163, 1166, 1169, 1172
ApoI RAATTY 1 cut(s) 1488
AsiGI ACCGGT 1 cut(s) 825
Asp700I GAANNNNTTC 3 cut(s) 191, 654, 1527
AspLEI GCGC 2 cut(s) 361, 994
AspS9I GGNCC 3 cut(s) 148, 1322, 1435
AsuHPI GGTGA 2 cut(s) 875, 1136
AvaI CYCGRG 1 cut(s) 1029
AvaII GGWCC 1 cut(s) 1322
BaeGI GKGCMC 1 cut(s) 732
BarI GAAGNNNNNNTAC 4 cut(s) 617, 649, 674, 706
Bbv12I GWGCWC 2 cut(s) 732, 1458
BbvI GCAGC 6 cut(s) 84, 1147, 1150, 1153, 1159, 1181
BccI CCATC 4 cut(s) 494, 1004, 1146, 1213
BcgI CGANNNNNNTGC 2 cut(s) 719, 753
BciT130I CCWGG 1 cut(s) 1317
BclI TGATCA 1 cut(s) 336
BcoDI GTCTC 5 cut(s) 220, 377, 430, 464, 746
BcuI ACTAGT 2 cut(s) 1106, 1219
BfaI CTAG 6 cut(s) 47, 545, 1107, 1220, 1281, 1612
BfmI CTRYAG 3 cut(s) 858, 1167, 1472
BfoI RGCGCY 1 cut(s) 995
BfuAI ACCTGC 4 cut(s) 851, 881, 983, 1333
BisI GCNGC 9 cut(s) 11, 73, 1161, 1164, 1167, 1170, 1173, 1242, 1402
BlpI GCTNAGC 1 cut(s) 29
BlsI GCNGC 9 cut(s) 12, 74, 1162, 1165, 1168, 1171, 1174, 1243, 1403
BmcAI AGTACT 1 cut(s) 1285
Bme1390I CCNGG 1 cut(s) 1317
Bme18I GGWCC 1 cut(s) 1322
BmeT110I CYCGRG 1 cut(s) 1029
BmgT120I GGNCC 3 cut(s) 148, 1322, 1435
BmiI GGNNCC 4 cut(s) 43, 830, 1381, 1530
BmrFI CCNGG 1 cut(s) 1317
BmsI GCATC 4 cut(s) 826, 885, 1045, 1184
BpmI CTGGAG 1 cut(s) 1451
Bpu1102I GCTNAGC 1 cut(s) 29
BpuEI CTTGAG 2 cut(s) 1080, 1472
BsaAI YACGTR 1 cut(s) 984
BsaBI GATNNNNATC 2 cut(s) 1059, 1466
BsaJI CCNNGG 4 cut(s) 96, 196, 1315, 1509
BsaWI WCCGGW 1 cut(s) 825
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 214, 417, 1010, 1376
Bse118I RCCGGY 1 cut(s) 825
Bse1I ACTGG 2 cut(s) 82, 725
Bse3DI GCAATG 1 cut(s) 1337
Bse8I GATNNNNATC 2 cut(s) 1059, 1466
BseBI CCWGG 1 cut(s) 1317
BseDI CCNNGG 4 cut(s) 96, 196, 1315, 1509
BseGI GGATG 1 cut(s) 1606
BseJI GATNNNNATC 2 cut(s) 1059, 1466
BseLI CCNNNNNNNGG 5 cut(s) 97, 214, 417, 1010, 1376
BseMI GCAATG 1 cut(s) 1337
BseMII CTCAG 2 cut(s) 512, 1439
BseNI ACTGG 2 cut(s) 82, 725
BseSI GKGCMC 1 cut(s) 732
BseXI GCAGC 6 cut(s) 84, 1147, 1150, 1153, 1159, 1181
Bsh1236I CGCG 2 cut(s) 23, 741
BshFI GGCC 3 cut(s) 81, 150, 1436
BshTI ACCGGT 1 cut(s) 825
BsiHKAI GWGCWC 2 cut(s) 732, 1458
BsiHKCI CYCGRG 1 cut(s) 1029
BsiSI CCGG 3 cut(s) 215, 286, 826
BslFI GGGAC 4 cut(s) 394, 624, 674, 1084
BslI CCNNNNNNNGG 5 cut(s) 97, 214, 417, 1010, 1376
BsmAI GTCTC 5 cut(s) 220, 377, 430, 464, 746
BsmFI GGGAC 4 cut(s) 394, 624, 674, 1084
BsmI GAATGC 1 cut(s) 527
BsnI GGCC 3 cut(s) 81, 150, 1436
BsoBI CYCGRG 1 cut(s) 1029
Bsp1286I GDGCHC 2 cut(s) 732, 1458
Bsp143I GATC 7 cut(s) 120, 336, 540, 804, 950, 1043, 1467
Bsp1720I GCTNAGC 1 cut(s) 29
Bsp19I CCATGG 1 cut(s) 96
Bsp68I TCGCGA 1 cut(s) 741
BspACI CCGC 6 cut(s) 23, 93, 1242, 1352, 1401, 1587
BspANI GGCC 3 cut(s) 81, 150, 1436
BspCNI CTCAG 2 cut(s) 511, 1438
BspFNI CGCG 2 cut(s) 23, 741
BspHI TCATGA 2 cut(s) 1141, 1594
BspLI GGNNCC 4 cut(s) 43, 830, 1381, 1530
BspMAI CTGCAG 3 cut(s) 862, 1171, 1476
BspMI ACCTGC 4 cut(s) 851, 881, 983, 1333
BspPI GGATC 3 cut(s) 535, 1051, 1462
BspQI GCTCTTC 1 cut(s) 843
BsrDI GCAATG 1 cut(s) 1337
BsrFI RCCGGY 1 cut(s) 825
BsrI ACTGG 2 cut(s) 82, 725
BssAI RCCGGY 1 cut(s) 825
BssECI CCNNGG 4 cut(s) 96, 196, 1315, 1509
BssMI GATC 7 cut(s) 120, 336, 540, 804, 950, 1043, 1467
BssNAI GTATAC 1 cut(s) 471
BssT1I CCWWGG 2 cut(s) 96, 1509
Bst1107I GTATAC 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 1317
Bst4CI ACNGT 2 cut(s) 561, 747
Bst6I CTCTTC 3 cut(s) 359, 843, 885
BstBAI YACGTR 1 cut(s) 984
BstC8I GCNNGC 4 cut(s) 722, 943, 947, 1158
BstDEI CTNAG 4 cut(s) 29, 183, 498, 1425
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 1606
BstFNI CGCG 2 cut(s) 23, 741
BstH2I RGCGCY 1 cut(s) 995
BstHHI GCGC 2 cut(s) 361, 994
BstKTI GATC 7 cut(s) 123, 339, 543, 807, 953, 1046, 1470
BstMAI GTCTC 5 cut(s) 220, 377, 430, 464, 746
BstMBI GATC 7 cut(s) 120, 336, 540, 804, 950, 1043, 1467
BstMWI GCNNNNNNNGC 8 cut(s) 78, 866, 984, 1166, 1169, 1172, 1238, 1480
BstNI CCWGG 1 cut(s) 1317
BstNSI RCATGY 2 cut(s) 632, 1363
BstSCI CCNGG 1 cut(s) 1315
BstSFI CTRYAG 3 cut(s) 858, 1167, 1472
BstSLI GKGCMC 1 cut(s) 732
BstUI CGCG 2 cut(s) 23, 741
BstV1I GCAGC 6 cut(s) 84, 1147, 1150, 1153, 1159, 1181
BstX2I RGATCY 1 cut(s) 1467
BstYI RGATCY 1 cut(s) 1467
BstZ17I GTATAC 1 cut(s) 471
BsuRI GGCC 3 cut(s) 81, 150, 1436
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 1606
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 3 cut(s) 321, 557, 732
BtuMI TCGCGA 1 cut(s) 741
BveI ACCTGC 4 cut(s) 851, 881, 983, 1333
Cac8I GCNNGC 4 cut(s) 722, 943, 947, 1158
CciI TCATGA 2 cut(s) 1141, 1594
CfoI GCGC 2 cut(s) 361, 994
Cfr10I RCCGGY 1 cut(s) 825
Cfr13I GGNCC 3 cut(s) 148, 1322, 1435
CseI GACGC 1 cut(s) 875
Csp6I GTAC 7 cut(s) 376, 535, 712, 1001, 1023, 1086, 1284
CspAI ACCGGT 1 cut(s) 825
CviQI GTAC 7 cut(s) 376, 535, 712, 1001, 1023, 1086, 1284
DdeI CTNAG 4 cut(s) 29, 183, 498, 1425
DpnI GATC 7 cut(s) 122, 338, 542, 806, 952, 1045, 1469
DpnII GATC 7 cut(s) 120, 336, 540, 804, 950, 1043, 1467
DrdI GACNNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 1 cut(s) 391
Eam1104I CTCTTC 3 cut(s) 359, 843, 885
EarI CTCTTC 3 cut(s) 359, 843, 885
Eco130I CCWWGG 2 cut(s) 96, 1509
Eco47I GGWCC 1 cut(s) 1322
Eco47III AGCGCT 1 cut(s) 993
Eco57I CTGAAG 1 cut(s) 867
Eco88I CYCGRG 1 cut(s) 1029
EcoRII CCWGG 1 cut(s) 1315
EcoT14I CCWWGG 2 cut(s) 96, 1509
EcoT22I ATGCAT 1 cut(s) 67
ErhI CCWWGG 2 cut(s) 96, 1509
FaqI GGGAC 4 cut(s) 394, 624, 674, 1084
FauI CCCGC 3 cut(s) 16, 100, 1580
FauNDI CATATG 1 cut(s) 1266
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 2 cut(s) 470, 633
Fnu4HI GCNGC 9 cut(s) 11, 73, 1161, 1164, 1167, 1170, 1173, 1242, 1402
Fsp4HI GCNGC 9 cut(s) 11, 73, 1161, 1164, 1167, 1170, 1173, 1242, 1402
FspBI CTAG 6 cut(s) 47, 545, 1107, 1220, 1281, 1612
GlaI GCGC 2 cut(s) 360, 993
GluI GCNGC 9 cut(s) 11, 73, 1161, 1164, 1167, 1170, 1173, 1242, 1402
GsuI CTGGAG 1 cut(s) 1451
HaeII RGCGCY 1 cut(s) 995
HaeIII GGCC 3 cut(s) 81, 150, 1436
HapII CCGG 3 cut(s) 215, 286, 826
HgaI GACGC 1 cut(s) 875
HhaI GCGC 2 cut(s) 361, 994
Hin6I GCGC 2 cut(s) 359, 992
HinP1I GCGC 2 cut(s) 359, 992
HincII GTYRAC 2 cut(s) 564, 1609
HindII GTYRAC 2 cut(s) 564, 1609
HinfI GANTC 7 cut(s) 262, 496, 736, 772, 1274, 1477, 1577
HpaII CCGG 3 cut(s) 215, 286, 826
HphI GGTGA 2 cut(s) 875, 1136
Hpy166II GTNNAC 8 cut(s) 103, 471, 564, 634, 730, 923, 1322, 1609
Hpy188I TCNGA 6 cut(s) 209, 420, 501, 1017, 1054, 1576
Hpy188III TCNNGA 9 cut(s) 659, 700, 740, 760, 887, 1007, 1142, 1451, 1595
Hpy8I GTNNAC 8 cut(s) 103, 471, 564, 634, 730, 923, 1322, 1609
HpyAV CCTTC 2 cut(s) 516, 1517
HpyCH4III ACNGT 2 cut(s) 561, 747
HpyCH4IV ACGT 1 cut(s) 983
HpyF10VI GCNNNNNNNGC 8 cut(s) 78, 866, 984, 1166, 1169, 1172, 1238, 1480
HpyF3I CTNAG 4 cut(s) 29, 183, 498, 1425
HpySE526I ACGT 1 cut(s) 983
HspAI GCGC 2 cut(s) 359, 992
Ksp22I TGATCA 1 cut(s) 336
Kzo9I GATC 7 cut(s) 120, 336, 540, 804, 950, 1043, 1467
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 4 cut(s) 672, 1055, 1379, 1488
Lsp1109I GCAGC 6 cut(s) 84, 1147, 1150, 1153, 1159, 1181
LweI GCATC 4 cut(s) 826, 885, 1045, 1184
MaeI CTAG 6 cut(s) 47, 545, 1107, 1220, 1281, 1612
MaeII ACGT 1 cut(s) 983
MaeIII GTNAC 3 cut(s) 319, 593, 863
MalI GATC 7 cut(s) 122, 338, 542, 806, 952, 1045, 1469
MboI GATC 7 cut(s) 120, 336, 540, 804, 950, 1043, 1467
MboII GAAGA 5 cut(s) 128, 376, 685, 860, 902
MflI RGATCY 1 cut(s) 1467
MhlI GDGCHC 2 cut(s) 732, 1458
MluCI AATT 2 cut(s) 1488, 1582
MlyI GAGTC 2 cut(s) 490, 1268
MmeI TCCRAC 2 cut(s) 995, 1556
Mph1103I ATGCAT 1 cut(s) 67
MroXI GAANNNNTTC 3 cut(s) 191, 654, 1527
MseI TTAA 3 cut(s) 231, 768, 1257
MslI CAYNNNNRTG 2 cut(s) 602, 1599
MspA1I CMGCKG 2 cut(s) 1172, 1429
MspI CCGG 3 cut(s) 215, 286, 826
MspR9I CCNGG 1 cut(s) 1317
Mva1269I GAATGC 1 cut(s) 527
MvaI CCWGG 1 cut(s) 1317
MvnI CGCG 2 cut(s) 23, 741
MwoI GCNNNNNNNGC 8 cut(s) 78, 866, 984, 1166, 1169, 1172, 1238, 1480
NcoI CCATGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 1266
NdeII GATC 7 cut(s) 120, 336, 540, 804, 950, 1043, 1467
NlaIV GGNNCC 4 cut(s) 43, 830, 1381, 1530
NmeAIII GCCGAG 2 cut(s) 177, 1013
NmuCI GTSAC 3 cut(s) 319, 593, 863
NruI TCGCGA 1 cut(s) 741
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 2 cut(s) 632, 1363
PaeR7I CTCGAG 1 cut(s) 1029
PagI TCATGA 2 cut(s) 1141, 1594
PaqCI CACCTGC 1 cut(s) 851
PciI ACATGT 1 cut(s) 1359
PciSI GCTCTTC 1 cut(s) 843
PctI GAATGC 1 cut(s) 527
PdmI GAANNNNTTC 3 cut(s) 191, 654, 1527
PfeI GAWTC 5 cut(s) 262, 736, 772, 1477, 1577
PinAI ACCGGT 1 cut(s) 825
PkrI GCNGC 9 cut(s) 12, 74, 1162, 1165, 1168, 1171, 1174, 1243, 1403
PleI GAGTC 2 cut(s) 490, 1268
PpsI GAGTC 2 cut(s) 490, 1268
Ppu21I YACGTR 1 cut(s) 984
PscI ACATGT 1 cut(s) 1359
Psp6I CCWGG 1 cut(s) 1315
PspGI CCWGG 1 cut(s) 1315
PspN4I GGNNCC 4 cut(s) 43, 830, 1381, 1530
PspPI GGNCC 3 cut(s) 148, 1322, 1435
PspXI VCTCGAGB 1 cut(s) 1029
PstI CTGCAG 3 cut(s) 862, 1171, 1476
PsuI RGATCY 1 cut(s) 1467
PvuII CAGCTG 2 cut(s) 1172, 1429
RruI TCGCGA 1 cut(s) 741
RsaI GTAC 7 cut(s) 377, 536, 713, 1002, 1024, 1087, 1285
RsaNI GTAC 7 cut(s) 376, 535, 712, 1001, 1023, 1086, 1284
RseI CAYNNNNRTG 2 cut(s) 602, 1599
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 3 cut(s) 231, 768, 1257
SatI GCNGC 9 cut(s) 11, 73, 1161, 1164, 1167, 1170, 1173, 1242, 1402
Sau3AI GATC 7 cut(s) 120, 336, 540, 804, 950, 1043, 1467
Sau96I GGNCC 3 cut(s) 148, 1322, 1435
ScaI AGTACT 1 cut(s) 1285
SchI GAGTC 2 cut(s) 490, 1268
ScrFI CCNGG 1 cut(s) 1317
SduI GDGCHC 2 cut(s) 732, 1458
SfaNI GCATC 4 cut(s) 826, 885, 1045, 1184
SfcI CTRYAG 3 cut(s) 858, 1167, 1472
Sfr274I CTCGAG 1 cut(s) 1029
SinI GGWCC 1 cut(s) 1322
SlaI CTCGAG 1 cut(s) 1029
SmiMI CAYNNNNRTG 2 cut(s) 602, 1599
SmlI CTYRAG 3 cut(s) 1029, 1095, 1451
SmoI CTYRAG 3 cut(s) 1029, 1095, 1451
SpeI ACTAGT 2 cut(s) 1106, 1219
Sse9I AATT 2 cut(s) 1488, 1582
SsiI CCGC 6 cut(s) 23, 93, 1242, 1352, 1401, 1587
SspMI CTAG 6 cut(s) 47, 545, 1107, 1220, 1281, 1612
StyD4I CCNGG 1 cut(s) 1315
StyI CCWWGG 2 cut(s) 96, 1509
TaaI ACNGT 2 cut(s) 561, 747
TaiI ACGT 1 cut(s) 986
TaqI TCGA 4 cut(s) 110, 531, 799, 1030
TasI AATT 2 cut(s) 1488, 1582
TatI WGTACW 4 cut(s) 375, 534, 711, 1283
TauI GCSGC 2 cut(s) 1244, 1404
TfiI GAWTC 5 cut(s) 262, 736, 772, 1477, 1577
Tru1I TTAA 3 cut(s) 231, 768, 1257
Tru9I TTAA 3 cut(s) 231, 768, 1257
TscAI CASTG 3 cut(s) 328, 564, 732
TseFI GTSAC 3 cut(s) 319, 593, 863
TseI GCWGC 7 cut(s) 10, 72, 1160, 1163, 1166, 1169, 1172
Tsp45I GTSAC 3 cut(s) 319, 593, 863
TspDTI ATGAA 6 cut(s) 685, 697, 833, 923, 1158, 1364
TspGWI ACGGA 2 cut(s) 381, 782
TspRI CASTG 3 cut(s) 328, 564, 732
VneI GTGCAC 1 cut(s) 728
VpaK11BI GGWCC 1 cut(s) 1322
XapI RAATTY 1 cut(s) 1488
XceI RCATGY 2 cut(s) 632, 1363
XcmI CCANNNNNNNNNTGG 2 cut(s) 124, 1516
XhoI CTCGAG 1 cut(s) 1029
XmiI GTMKAC 2 cut(s) 470, 633
XmnI GAANNNNTTC 3 cut(s) 191, 654, 1527
XspI CTAG 6 cut(s) 47, 545, 1107, 1220, 1281, 1612
ZrmI AGTACT 1 cut(s) 1285
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.