MD01G1235000.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003784
Physical Location & Seq
Reverse (-)
32262270 .. 32264115
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1235000.v1.1.491

Sequence Viewer

Length: 384 bp
ATGTGGAGATTTTTGATTGTTGACGTGCATAAGCTGAGGTACTATGGTGTGCCACTGCACTTGATACGTGAGCTCTATGAAACATTTAGGAACTTTAAGATTCGCATTGCTGATTACATTCGTTATCGAAAGCTCACATCAAACATGAATGATCAATTCCCAGATGCTACGCATGAAGAACTTAATGCAAGTGACGCAACTTGTATCATTTGCCGTGAAGAGATACAACAGCCAAGAAACTATTATGTGGACACCTTTTTCATGTGCCTTGCCTTCGATAATGGGACAGGCACAACGTCTCAGGATGGTGGAGTTGCGGGTGATCGTGTGAGCCAATATGAAGCCAGGCTCCGAGCTGCTGCTACTGCTGCATCGATATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000153 GO:0000209 GO:0000835 GO:0000836 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0023052 GO:0030163 GO:0030433 GO:0030968 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034620 GO:0034645 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070085 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071944 GO:0098796 GO:0098827 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1902494 GO:1990234 GO:1990381
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 map04120 map04141
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

128

Amino Acids

14.8

Weight (kDa)

6.28

Isoelectric Point (pI)

21.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_SYVN1_N PF25563 11 - 48 1.8e-10 E3 ubiquitin-protein ligase synoviolin-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 317
AfaI GTAC 1 cut(s) 41
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 1 cut(s) 344
AluBI AGCT 4 cut(s) 34, 73, 133, 356
AluI AGCT 4 cut(s) 34, 73, 133, 356
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 1 cut(s) 303
ApeKI GCWGC 3 cut(s) 356, 359, 368
AsuHPI GGTGA 1 cut(s) 332
BaeI ACNNNNGTAYC 2 cut(s) 31, 64
BanII GRGCYC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 75
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 3 cut(s) 343, 346, 355
BccI CCATC 1 cut(s) 299
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 1 cut(s) 346
BclI TGATCA 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 303
BisI GCNGC 3 cut(s) 357, 360, 369
BlsI GCNGC 3 cut(s) 358, 361, 370
Bme1390I CCNGG 1 cut(s) 346
BmgBI CACGTC 1 cut(s) 25
BmiI GGNNCC 1 cut(s) 350
BmrFI CCNGG 1 cut(s) 346
BmsI GCATC 2 cut(s) 154, 380
Bpu10I CCTNAGC 1 cut(s) 35
Bsa29I ATCGAT 1 cut(s) 374
BsaAI YACGTR 1 cut(s) 68
Bse3DI GCAATG 1 cut(s) 105
BseBI CCWGG 1 cut(s) 346
BseCI ATCGAT 1 cut(s) 374
BseGI GGATG 1 cut(s) 310
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 2 cut(s) 26, 314
BseXI GCAGC 3 cut(s) 343, 346, 355
BsgI GTGCAG 1 cut(s) 41
BshVI ATCGAT 1 cut(s) 374
BsiHKAI GWGCWC 1 cut(s) 75
BslFI GGGAC 1 cut(s) 298
BsmAI GTCTC 1 cut(s) 303
BsmBI CGTCTC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 2 cut(s) 151, 322
BspACI CCGC 1 cut(s) 317
BspCNI CTCAG 2 cut(s) 27, 313
BspDI ATCGAT 1 cut(s) 374
BspLI GGNNCC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 105
BssMI GATC 2 cut(s) 151, 322
Bst2UI CCWGG 1 cut(s) 346
Bst6I CTCTTC 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 68
BstDEI CTNAG 2 cut(s) 35, 300
BstF5I GGATG 1 cut(s) 310
BstKTI GATC 2 cut(s) 154, 325
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 2 cut(s) 151, 322
BstMWI GCNNNNNNNGC 3 cut(s) 194, 365, 368
BstNI CCWGG 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 344
BstV1I GCAGC 3 cut(s) 343, 346, 355
Bsu15I ATCGAT 1 cut(s) 374
BsuTUI ATCGAT 1 cut(s) 374
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 1 cut(s) 310
BtsI GCAGTG 1 cut(s) 53
BtsIMutI CAGTG 1 cut(s) 53
ClaI ATCGAT 1 cut(s) 374
CseI GACGC 1 cut(s) 203
Csp6I GTAC 1 cut(s) 40
CspCI CAANNNNNGTGG 2 cut(s) 42, 77
CviAII CATG 3 cut(s) 145, 173, 262
CviJI RGCY 8 cut(s) 34, 73, 133, 232, 333, 344, 349, 356
CviKI_1 RGCY 8 cut(s) 34, 73, 133, 232, 333, 344, 349, 356
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 2 cut(s) 35, 300
DpnI GATC 2 cut(s) 153, 324
DpnII GATC 2 cut(s) 151, 322
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 73
Eco24I GRGCYC 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 344
EcoT38I GRGCYC 1 cut(s) 75
Esp3I CGTCTC 1 cut(s) 303
FaeI CATG 3 cut(s) 148, 176, 265
FaiI YATR 9 cut(s) 30, 45, 78, 146, 174, 246, 263, 339, 379
FaqI GGGAC 1 cut(s) 298
FatI CATG 3 cut(s) 144, 172, 261
FauI CCCGC 1 cut(s) 310
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 3 cut(s) 357, 360, 369
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 3 cut(s) 357, 360, 369
GluI GCNGC 3 cut(s) 357, 360, 369
HgaI GACGC 1 cut(s) 203
Hin1II CATG 3 cut(s) 148, 176, 265
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HinfI GANTC 1 cut(s) 100
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 2 cut(s) 22, 250
Hpy188I TCNGA 1 cut(s) 353
Hpy188III TCNNGA 1 cut(s) 302
Hpy8I GTNNAC 2 cut(s) 22, 250
HpyAV CCTTC 1 cut(s) 283
HpyCH4IV ACGT 3 cut(s) 24, 67, 296
HpyCH4V TGCA 4 cut(s) 28, 58, 188, 371
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 365, 368
HpyF3I CTNAG 2 cut(s) 35, 300
HpySE526I ACGT 3 cut(s) 24, 67, 296
Hsp92II CATG 3 cut(s) 148, 176, 265
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 2 cut(s) 151, 322
LmnI GCTCC 1 cut(s) 354
LpnPI CCDG 5 cut(s) 174, 273, 287, 331, 358
Lsp1109I GCAGC 3 cut(s) 343, 346, 355
LweI GCATC 2 cut(s) 154, 380
MaeII ACGT 3 cut(s) 24, 67, 296
MaeIII GTNAC 1 cut(s) 191
MalI GATC 2 cut(s) 153, 324
MboI GATC 2 cut(s) 151, 322
MboII GAAGA 2 cut(s) 188, 230
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 1 cut(s) 155
MnlI CCTC 1 cut(s) 30
MseI TTAA 2 cut(s) 96, 183
MspR9I CCNGG 1 cut(s) 346
MvaI CCWGG 1 cut(s) 346
MwoI GCNNNNNNNGC 3 cut(s) 194, 365, 368
NdeII GATC 2 cut(s) 151, 322
NlaIII CATG 3 cut(s) 148, 176, 265
NlaIV GGNNCC 1 cut(s) 350
NmuCI GTSAC 1 cut(s) 191
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 3 cut(s) 358, 361, 370
Ppu21I YACGTR 1 cut(s) 68
Psp124BI GAGCTC 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 344
PspGI CCWGG 1 cut(s) 344
PspN4I GGNNCC 1 cut(s) 350
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 2 cut(s) 96, 183
SatI GCNGC 3 cut(s) 357, 360, 369
Sau3AI GATC 2 cut(s) 151, 322
ScrFI CCNGG 1 cut(s) 346
SduI GDGCHC 1 cut(s) 75
SetI ASST 9 cut(s) 27, 36, 41, 70, 75, 135, 257, 299, 358
SfaNI GCATC 2 cut(s) 154, 380
Sse9I AATT 1 cut(s) 155
SsiI CCGC 1 cut(s) 317
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 344
TaiI ACGT 3 cut(s) 27, 70, 299
TaqI TCGA 3 cut(s) 127, 276, 374
TasI AATT 1 cut(s) 155
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 2 cut(s) 96, 183
Tru9I TTAA 2 cut(s) 96, 183
TscAI CASTG 1 cut(s) 60
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 3 cut(s) 356, 359, 368
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 5 cut(s) 93, 161, 189, 250, 354
TspRI CASTG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.