pycom17g03700

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003784
Physical Location & Seq
Forward (+)
2491295 .. 2493060
1766 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g03700.1

Sequence Viewer

Length: 834 bp
ATGTTTGGCTCCCATGTTGCGGTGAATTTTGTCCCACCCCAGGTACTATGGTGTGCCACTGCACTTGAAACATTTAGAAACTTTAAGATTGGCATTGCTGATTACATTGGTTATCGAAAGCTCACATCAAACATGAATGATCGATTCCCAGATGCTACGCATGAAGAGCTTAATGAAAGTGACGCAACTTGTATCATTTGCCGTGAAGAGATGACAACAGCCAAGAAACTATTATGTGGACACCTTTTTCATGTGTCGGTTGCGGCCCTAGTTGTACCTCCTGACAGTGGGAAAGGTACAGCTGGCGGACATATAGGGGTTCACGGTCAGATTCTCATCAACAGGGCACAACGGCTCAGAATGGTGGAGTTGCTGGTGATGGTGTCTCCTGGATATGCAGCACTTCCTCAGGTTCAAAGACATATGGTTGTCGACTCCTCTAATACTGAATCTAGTGGAGAAGCTTGTAGTGGACAATCACAACCGCAGCTTGCAACCCCTGGTGGACCTGCAAACTTGTCTTTCTCTCAGTTTCCGCCTTACATGTTTGTCCCTGGAACCAACGTGGCCTATGGGGAGAGGTCGGGCAGCGATCCAAATCTGACAGCTTCTCAACTGGAGGCCCAGAAAAAAGTTTCTTCAGCACCAGATACAGTAACTGCTTTTCTTATACAGATCCTGCAGAATCAGCTCCAAGTTTTACAGAAACCAAAACCAAAGGAAAGTGTGGATATGGGTCCAACAAGGGCTTCTTTGGATGGCAAAGGAAAGAGTGTTGCAGCTTCTTCATCTGTTTCAGATTGTGGACGTCATGAAGATGGGGTTGATTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000153 GO:0000209 GO:0000835 GO:0000836 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006486 GO:0006487 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006986 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016567 GO:0016740 GO:0018193 GO:0018196 GO:0018279 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0023052 GO:0030163 GO:0030433 GO:0030968 GO:0031984 GO:0032446 GO:0032991 GO:0033554 GO:0034620 GO:0034645 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044322 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051082 GO:0051603 GO:0051716 GO:0061630 GO:0061659 GO:0065007 GO:0070085 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071944 GO:0098796 GO:0098827 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1902494 GO:1990234 GO:1990381
KEGG Pathways
Metabolic & Signaling
ko04120 ko04141 map04120 map04141
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

278

Amino Acids

29.63

Weight (kDa)

6.23

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 811
Acc36I ACCTGC 1 cut(s) 517
AccI GTMKAC 1 cut(s) 432
AciI CCGC 5 cut(s) 20, 263, 306, 485, 536
AclWI GGATC 2 cut(s) 587, 670
AcsI RAATTY 1 cut(s) 25
AcuI CTGAAG 1 cut(s) 624
AcyI GRCGYC 1 cut(s) 808
AfaI GTAC 3 cut(s) 45, 276, 298
AfiI CCNNNNNNNGG 3 cut(s) 19, 40, 287
AflIII ACRYGT 1 cut(s) 543
AgsI TTSAA 2 cut(s) 68, 416
AjnI CCWGG 4 cut(s) 39, 388, 499, 553
AjuI GAANNNNNNNTTGG 2 cut(s) 733, 765
AluBI AGCT 8 cut(s) 121, 169, 302, 464, 490, 608, 691, 782
AluI AGCT 8 cut(s) 121, 169, 302, 464, 490, 608, 691, 782
Alw26I GTCTC 1 cut(s) 390
AlwI GGATC 2 cut(s) 587, 670
AlwNI CAGNNNCTG 2 cut(s) 659, 679
AoxI GGCC 3 cut(s) 264, 567, 621
ApeKI GCWGC 4 cut(s) 398, 487, 588, 779
ApoI RAATTY 1 cut(s) 25
AspS9I GGNCC 4 cut(s) 265, 506, 622, 737
AsuHPI GGTGA 2 cut(s) 34, 388
AvaII GGWCC 2 cut(s) 506, 737
AxyI CCTNAGG 1 cut(s) 408
BaeGI GKGCMC 1 cut(s) 349
BaeI ACNNNNGTAYC 2 cut(s) 35, 68
BbvI GCAGC 4 cut(s) 410, 499, 600, 791
BccI CCATC 3 cut(s) 373, 752, 812
BceAI ACGGC 2 cut(s) 186, 368
BciT130I CCWGG 4 cut(s) 41, 390, 501, 555
BcoDI GTCTC 1 cut(s) 390
BfaI CTAG 2 cut(s) 269, 453
BfmI CTRYAG 1 cut(s) 680
BfuAI ACCTGC 1 cut(s) 517
BisI GCNGC 5 cut(s) 264, 399, 488, 589, 780
BlsI GCNGC 5 cut(s) 265, 400, 489, 590, 781
Bme1390I CCNGG 4 cut(s) 41, 390, 501, 555
Bme18I GGWCC 2 cut(s) 506, 737
BmgT120I GGNCC 4 cut(s) 265, 506, 622, 737
BmiI GGNNCC 3 cut(s) 10, 559, 738
BmrFI CCNGG 4 cut(s) 41, 390, 501, 555
BmsI GCATC 1 cut(s) 142
BpmI CTGGAG 1 cut(s) 638
Bsa29I ATCGAT 1 cut(s) 142
BsaBI GATNNNNATC 2 cut(s) 335, 597
BsaHI GRCGYC 1 cut(s) 808
BsaJI CCNNGG 3 cut(s) 39, 499, 553
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 40, 287
Bse1I ACTGG 1 cut(s) 621
Bse21I CCTNAGG 1 cut(s) 408
Bse3DI GCAATG 1 cut(s) 93
Bse8I GATNNNNATC 2 cut(s) 335, 597
BseBI CCWGG 4 cut(s) 41, 390, 501, 555
BseCI ATCGAT 1 cut(s) 142
BseDI CCNNGG 3 cut(s) 39, 499, 553
BseGI GGATG 1 cut(s) 763
BseJI GATNNNNATC 2 cut(s) 335, 597
BseLI CCNNNNNNNGG 3 cut(s) 19, 40, 287
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 3 cut(s) 370, 422, 542
BseNI ACTGG 1 cut(s) 621
BseRI GAGGAG 1 cut(s) 427
BseSI GKGCMC 1 cut(s) 349
BseXI GCAGC 4 cut(s) 410, 499, 600, 791
BsgI GTGCAG 1 cut(s) 45
BshFI GGCC 3 cut(s) 266, 569, 623
BshVI ATCGAT 1 cut(s) 142
BslFI GGGAC 2 cut(s) 17, 536
BslI CCNNNNNNNGG 3 cut(s) 19, 40, 287
BsmAI GTCTC 1 cut(s) 390
BsmFI GGGAC 2 cut(s) 17, 536
BsnI GGCC 3 cut(s) 266, 569, 623
Bsp1286I GDGCHC 1 cut(s) 349
Bsp143I GATC 3 cut(s) 139, 592, 675
BspACI CCGC 5 cut(s) 20, 263, 306, 485, 536
BspANI GGCC 3 cut(s) 266, 569, 623
BspCNI CTCAG 3 cut(s) 369, 421, 541
BspDI ATCGAT 1 cut(s) 142
BspHI TCATGA 1 cut(s) 811
BspLI GGNNCC 3 cut(s) 10, 559, 738
BspMAI CTGCAG 1 cut(s) 684
BspMI ACCTGC 1 cut(s) 517
BspPI GGATC 2 cut(s) 587, 670
BspQI GCTCTTC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 93
BsrI ACTGG 1 cut(s) 621
BssECI CCNNGG 3 cut(s) 39, 499, 553
BssMI GATC 3 cut(s) 139, 592, 675
BssNI GRCGYC 1 cut(s) 808
Bst2UI CCWGG 4 cut(s) 41, 390, 501, 555
Bst4CI ACNGT 3 cut(s) 287, 326, 655
Bst6I CTCTTC 2 cut(s) 159, 201
BstACI GRCGYC 1 cut(s) 808
BstC8I GCNNGC 2 cut(s) 304, 492
BstDEI CTNAG 3 cut(s) 356, 408, 528
BstF5I GGATG 1 cut(s) 763
BstKTI GATC 3 cut(s) 142, 595, 678
BstMAI GTCTC 1 cut(s) 390
BstMBI GATC 3 cut(s) 139, 592, 675
BstMWI GCNNNNNNNGC 2 cut(s) 166, 688
BstNI CCWGG 4 cut(s) 41, 390, 501, 555
BstNSI RCATGY 1 cut(s) 547
BstSCI CCNGG 4 cut(s) 39, 388, 499, 553
BstSFI CTRYAG 1 cut(s) 680
BstSLI GKGCMC 1 cut(s) 349
BstV1I GCAGC 4 cut(s) 410, 499, 600, 791
BstX2I RGATCY 1 cut(s) 675
BstYI RGATCY 1 cut(s) 675
Bsu15I ATCGAT 1 cut(s) 142
Bsu36I CCTNAGG 1 cut(s) 408
BsuRI GGCC 3 cut(s) 266, 569, 623
BsuTUI ATCGAT 1 cut(s) 142
BtsCI GGATG 1 cut(s) 763
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 2 cut(s) 57, 292
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 2 cut(s) 304, 492
CaiI CAGNNNCTG 2 cut(s) 659, 679
CciI TCATGA 1 cut(s) 811
Cfr13I GGNCC 4 cut(s) 265, 506, 622, 737
ClaI ATCGAT 1 cut(s) 142
CseI GACGC 1 cut(s) 191
Csp6I GTAC 3 cut(s) 44, 275, 297
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviAII CATG 6 cut(s) 14, 133, 161, 251, 544, 812
CviQI GTAC 3 cut(s) 44, 275, 297
DdeI CTNAG 3 cut(s) 356, 408, 528
DpnI GATC 3 cut(s) 141, 594, 677
DpnII GATC 3 cut(s) 139, 592, 675
Eam1104I CTCTTC 2 cut(s) 159, 201
EarI CTCTTC 2 cut(s) 159, 201
EciI GGCGGA 2 cut(s) 321, 525
Eco47I GGWCC 2 cut(s) 506, 737
Eco57I CTGAAG 1 cut(s) 624
Eco81I CCTNAGG 1 cut(s) 408
EcoRII CCWGG 4 cut(s) 39, 388, 499, 553
FaeI CATG 6 cut(s) 17, 136, 164, 254, 547, 815
FalI AAGNNNNNCTT 2 cut(s) 736, 768
FaqI GGGAC 2 cut(s) 17, 536
FatI CATG 6 cut(s) 13, 132, 160, 250, 543, 811
FauNDI CATATG 1 cut(s) 423
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 5 cut(s) 264, 399, 488, 589, 780
FokI GGATG 1 cut(s) 770
Fsp4HI GCNGC 5 cut(s) 264, 399, 488, 589, 780
FspBI CTAG 2 cut(s) 269, 453
GluI GCNGC 5 cut(s) 264, 399, 488, 589, 780
GsuI CTGGAG 1 cut(s) 638
HaeIII GGCC 3 cut(s) 266, 569, 623
HgaI GACGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 808
Hin1II CATG 6 cut(s) 17, 136, 164, 254, 547, 815
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 6 cut(s) 144, 331, 434, 449, 685, 827
HphI GGTGA 2 cut(s) 34, 388
Hpy166II GTNNAC 6 cut(s) 239, 322, 433, 473, 506, 806
Hpy188I TCNGA 4 cut(s) 330, 359, 603, 799
Hpy188III TCNNGA 2 cut(s) 281, 812
Hpy8I GTNNAC 6 cut(s) 239, 322, 433, 473, 506, 806
HpyCH4III ACNGT 3 cut(s) 287, 326, 655
HpyCH4IV ACGT 2 cut(s) 564, 808
HpyCH4V TGCA 6 cut(s) 62, 398, 494, 512, 682, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 688
HpyF3I CTNAG 3 cut(s) 356, 408, 528
HpySE526I ACGT 2 cut(s) 564, 808
Hsp92I GRCGYC 1 cut(s) 808
Hsp92II CATG 6 cut(s) 17, 136, 164, 254, 547, 815
Kzo9I GATC 3 cut(s) 139, 592, 675
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 2 cut(s) 14, 696
Lsp1109I GCAGC 4 cut(s) 410, 499, 600, 791
LweI GCATC 1 cut(s) 142
MaeI CTAG 2 cut(s) 269, 453
MaeII ACGT 2 cut(s) 564, 808
MaeIII GTNAC 2 cut(s) 179, 655
MalI GATC 3 cut(s) 141, 594, 677
MboI GATC 3 cut(s) 139, 592, 675
MboII GAAGA 5 cut(s) 176, 218, 630, 777, 827
MflI RGATCY 1 cut(s) 675
MhlI GDGCHC 1 cut(s) 349
MluCI AATT 1 cut(s) 25
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 1 cut(s) 764
MnlI CCTC 5 cut(s) 288, 417, 448, 573, 613
MseI TTAA 2 cut(s) 84, 171
MslI CAYNNNNRTG 1 cut(s) 816
MspA1I CMGCKG 1 cut(s) 302
MspR9I CCNGG 4 cut(s) 41, 390, 501, 555
MvaI CCWGG 4 cut(s) 41, 390, 501, 555
MwoI GCNNNNNNNGC 2 cut(s) 166, 688
NdeI CATATG 1 cut(s) 423
NdeII GATC 3 cut(s) 139, 592, 675
NlaIII CATG 6 cut(s) 17, 136, 164, 254, 547, 815
NlaIV GGNNCC 3 cut(s) 10, 559, 738
NmuCI GTSAC 1 cut(s) 179
NspI RCATGY 1 cut(s) 547
PagI TCATGA 1 cut(s) 811
PciI ACATGT 1 cut(s) 543
PciSI GCTCTTC 1 cut(s) 159
PfeI GAWTC 5 cut(s) 144, 331, 449, 685, 827
PfoI TCCNGGA 1 cut(s) 388
PkrI GCNGC 5 cut(s) 265, 400, 489, 590, 781
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
PscI ACATGT 1 cut(s) 543
Psp6I CCWGG 4 cut(s) 39, 388, 499, 553
PspGI CCWGG 4 cut(s) 39, 388, 499, 553
PspN4I GGNNCC 3 cut(s) 10, 559, 738
PspPI GGNCC 4 cut(s) 265, 506, 622, 737
PstI CTGCAG 1 cut(s) 684
PstNI CAGNNNCTG 2 cut(s) 659, 679
PsuI RGATCY 1 cut(s) 675
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 3 cut(s) 45, 276, 298
RsaNI GTAC 3 cut(s) 44, 275, 297
RseI CAYNNNNRTG 1 cut(s) 816
SalI GTCGAC 1 cut(s) 431
SapI GCTCTTC 1 cut(s) 159
SaqAI TTAA 2 cut(s) 84, 171
SatI GCNGC 5 cut(s) 264, 399, 488, 589, 780
Sau3AI GATC 3 cut(s) 139, 592, 675
Sau96I GGNCC 4 cut(s) 265, 506, 622, 737
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 4 cut(s) 41, 390, 501, 555
SduI GDGCHC 1 cut(s) 349
SfaNI GCATC 1 cut(s) 142
SfcI CTRYAG 1 cut(s) 680
SinI GGWCC 2 cut(s) 506, 737
SmiMI CAYNNNNRTG 1 cut(s) 816
Sse9I AATT 1 cut(s) 25
SsiI CCGC 5 cut(s) 20, 263, 306, 485, 536
SspMI CTAG 2 cut(s) 269, 453
StyD4I CCNGG 4 cut(s) 39, 388, 499, 553
TaaI ACNGT 3 cut(s) 287, 326, 655
TaiI ACGT 2 cut(s) 567, 811
TaqI TCGA 3 cut(s) 115, 142, 432
TasI AATT 1 cut(s) 25
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 5 cut(s) 144, 331, 449, 685, 827
Tru1I TTAA 2 cut(s) 84, 171
Tru9I TTAA 2 cut(s) 84, 171
TscAI CASTG 2 cut(s) 64, 292
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 4 cut(s) 398, 487, 588, 779
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 6 cut(s) 149, 177, 189, 239, 777, 828
TspRI CASTG 2 cut(s) 64, 292
VpaK11BI GGWCC 2 cut(s) 506, 737
XapI RAATTY 1 cut(s) 25
XceI RCATGY 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 432
XspI CTAG 2 cut(s) 269, 453
ZraI GACGTC 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.