AT1G70280

NHL repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003391
Physical Location & Seq
Reverse (-)
26465804 .. 26468804
3001 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70280.1

Sequence Viewer

Length: 1344 bp
ATGGTGAAATTCGAAAATGGGTATAGTGTAGAGACTGTGTTTGATGGAAGTAAACTTGGTATTGAACCGTATTCTATTGAAGTCTTACCAAACGGAGAGTTACTTATTCTTGATTCTGAGAACAGTAACATCTACAAGATCTCATCTTCTCTTTCATTATATAGCAGGCCAAGACTGGTTACTGGCTCCCCTGAAGGATATCCAGGTCATGTTGACGGGAGATTACGTGATGCGAAGCTGAACCATCCTAAGGGGCTTACGGTTGATGACAGAGGAAATATATATGTGGCTGATACTGTGAATAATGCTATCCGGAAGATAAGTGAAGGAGGAGTTACAACTATTGCCGGGGGCAAAACGGTTCGAAATGGAGGGCATGTGGATGGACCAAGTGAAGATGCAAAGTTTTCAAATGATTTTGATGTTGTTTATGTTGGAAGTAGTTGTTCTCTGCTTGTTATTGACCGTGGAAACAAAGCTATCAGAGAGATTCAACTCCATTTCGATGACTGTGCTTATCAGTATGGAAGCGGGTTTCCTCTTGGGATTGCTGTTCTTGTTGCTGCAGGTTTCTTTGGTTATATGCTAGCTCTGTTGCAACGAAGAGTCGGCTCTATCGTGTCTTCTCATAATGATCAAGAAATGTTCGAAGCTGATCCTGATCAAAAGCCTATGAAACATTCCAGACCATCTCTGATTCCGGCTGGAGACGAGCAGCTAGAGAAACAAGAGGAAACATTTGTGGTATCGCTGGGAAAACTCGTTTCAAATGCTTGGGAGTCAGTTATGGAGATCCTTAGAAAAAAGCAGACAGGCACCAGCTTTCAGCAGTACCATGGGACAACAAAGCAGTCTGCTGCATTTAGCACATCAACTCCATGGCCAATTCAAGAGAGCTTTGTGATTCGAGACGAAGACGGACCTCCTCCTGTTGAGCCCAGGAACCCAACTCCAAGAAAGACTTATGCTTTTATGTCCAAAGATGCAGAGAAAATGCAGCAGCTACGTCAGAGCCGCGCATTCTACAGTAGCTGGGACGCTGAGTTCCCCAACCAGCAGCAACAGCAGCAAAAGCAGCACCAGAAGCATCAGCATCAGCAACAACAGCAGCAGCAGCATCGAAGGCATTACTCATCGATCCCGCATACTTATTATGAGCAGGACAGTGAAAAGTCTAATGAGATAGTCTTTGGAGCGGTCCAAGAACAGAGCAGCAAGCGTGTGGCTAAGCCTAAGCCGAAGCCTATAGAGTCTGGAGATCAAATGAATAATAATACTCAACAGAATCTTCACTACAGATCTCATTCTGTTAGTTACCCATATGGATACTATCCTAATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

447

Amino Acids

50.3

Weight (kDa)

6.44

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NHL PF01436 80 - 106 9.5e-06 NHL repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 557
AccB1I GGYRCC 1 cut(s) 815
AccBSI CCGCTC 1 cut(s) 1196
AccII CGCG 1 cut(s) 1017
AccIII TCCGGA 1 cut(s) 312
AciI CCGC 4 cut(s) 531, 1015, 1142, 1196
AclWI GGATC 3 cut(s) 650, 787, 1132
AcoI YGGCCR 1 cut(s) 881
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 213
AfaI GTAC 1 cut(s) 833
AfiI CCNNNNNNNGG 1 cut(s) 250
AgsI TTSAA 6 cut(s) 65, 80, 411, 494, 768, 890
AjnI CCWGG 2 cut(s) 202, 938
AloI GAACNNNNNNTCC 2 cut(s) 1028, 1060
AluBI AGCT 9 cut(s) 238, 479, 590, 653, 718, 822, 897, 1003, 1032
AluI AGCT 9 cut(s) 238, 479, 590, 653, 718, 822, 897, 1003, 1032
Alw26I GTCTC 3 cut(s) 26, 702, 903
AlwI GGATC 3 cut(s) 650, 787, 1132
AlwNI CAGNNNCTG 1 cut(s) 1032
Aor13HI TCCGGA 1 cut(s) 312
AoxI GGCC 2 cut(s) 167, 881
ApoI RAATTY 1 cut(s) 8
AspLEI GCGC 1 cut(s) 1019
AspS9I GGNCC 3 cut(s) 386, 920, 1198
AsuC2I CCSGG 1 cut(s) 349
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 3 cut(s) 12, 364, 648
AsuNHI GCTAGC 1 cut(s) 586
AvaII GGWCC 3 cut(s) 386, 920, 1198
AxyI CCTNAGG 1 cut(s) 249
BalI TGGCCA 1 cut(s) 883
BanI GGYRCC 1 cut(s) 815
BanII GRGCYC 1 cut(s) 939
BbsI GAAGAC 2 cut(s) 615, 921
BccI CCATC 4 cut(s) 38, 252, 377, 697
BcgI CGANNNNNNTGC 4 cut(s) 494, 528, 1100, 1134
BciT130I CCWGG 2 cut(s) 204, 940
BciVI GTATCC 1 cut(s) 1319
BclI TGATCA 2 cut(s) 634, 661
BcnI CCSGG 1 cut(s) 349
BcoDI GTCTC 3 cut(s) 26, 702, 903
BfaI CTAG 2 cut(s) 587, 719
BfmI CTRYAG 4 cut(s) 564, 1024, 1245, 1294
BfuAI ACCTGC 1 cut(s) 557
BfuI GTATCC 1 cut(s) 1319
BglII AGATCT 2 cut(s) 138, 1298
BlpI GCTNAGC 1 cut(s) 1227
Bme1390I CCNGG 3 cut(s) 204, 349, 940
Bme18I GGWCC 3 cut(s) 386, 920, 1198
BmgT120I GGNCC 3 cut(s) 386, 920, 1198
BmiI GGNNCC 3 cut(s) 187, 817, 944
BmrFI CCNGG 3 cut(s) 204, 349, 940
BmsI GCATC 6 cut(s) 220, 388, 973, 1096, 1102, 1126
BmtI GCTAGC 1 cut(s) 590
BpiI GAAGAC 2 cut(s) 615, 921
BpmI CTGGAG 2 cut(s) 726, 1275
Bpu10I CCTNAGC 1 cut(s) 1233
Bpu1102I GCTNAGC 1 cut(s) 1227
Bpu14I TTCGAA 3 cut(s) 12, 364, 648
BpuMI CCSGG 1 cut(s) 349
Bsa29I ATCGAT 1 cut(s) 1136
BsaAI YACGTR 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 660
BsaJI CCNNGG 5 cut(s) 348, 466, 835, 878, 938
BsaWI WCCGGW 1 cut(s) 312
BsaXI ACNNNNNCTCC 6 cut(s) 211, 241, 363, 393, 859, 889
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse1I ACTGG 2 cut(s) 180, 187
Bse21I CCTNAGG 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 660
BseAI TCCGGA 1 cut(s) 312
BseBI CCWGG 2 cut(s) 204, 940
BseCI ATCGAT 1 cut(s) 1136
BseDI CCNNGG 5 cut(s) 348, 466, 835, 878, 938
BseGI GGATG 2 cut(s) 244, 388
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMII CTCAG 2 cut(s) 108, 1032
BseNI ACTGG 2 cut(s) 180, 187
BseRI GAGGAG 2 cut(s) 345, 915
BseYI CCCAGC 2 cut(s) 751, 1032
Bsh1236I CGCG 1 cut(s) 1017
BshFI GGCC 2 cut(s) 169, 883
BshNI GGYRCC 1 cut(s) 815
BshVI ATCGAT 1 cut(s) 1136
BsiSI CCGG 3 cut(s) 313, 348, 701
BslFI GGGAC 2 cut(s) 853, 1049
BslI CCNNNNNNNGG 1 cut(s) 250
BsmAI GTCTC 3 cut(s) 26, 702, 903
BsmBI CGTCTC 2 cut(s) 702, 903
BsmFI GGGAC 2 cut(s) 853, 1049
BsmI GAATGC 1 cut(s) 1019
BsnI GGCC 2 cut(s) 169, 883
Bsp119I TTCGAA 3 cut(s) 12, 364, 648
Bsp1286I GDGCHC 1 cut(s) 939
Bsp13I TCCGGA 1 cut(s) 312
Bsp143I GATC 8 cut(s) 138, 634, 655, 661, 792, 1137, 1258, 1298
Bsp1720I GCTNAGC 1 cut(s) 1227
Bsp19I CCATGG 2 cut(s) 835, 878
BspACI CCGC 4 cut(s) 531, 1015, 1142, 1196
BspANI GGCC 2 cut(s) 169, 883
BspCNI CTCAG 2 cut(s) 109, 1033
BspDI ATCGAT 1 cut(s) 1136
BspEI TCCGGA 1 cut(s) 312
BspFNI CGCG 1 cut(s) 1017
BspLI GGNNCC 3 cut(s) 187, 817, 944
BspMAI CTGCAG 1 cut(s) 568
BspMI ACCTGC 1 cut(s) 557
BspOI GCTAGC 1 cut(s) 590
BspPI GGATC 3 cut(s) 650, 787, 1132
BspT104I TTCGAA 3 cut(s) 12, 364, 648
BspT107I GGYRCC 1 cut(s) 815
BsrBI CCGCTC 1 cut(s) 1196
BsrI ACTGG 2 cut(s) 180, 187
BssECI CCNNGG 5 cut(s) 348, 466, 835, 878, 938
BssMI GATC 8 cut(s) 138, 634, 655, 661, 792, 1137, 1258, 1298
BssT1I CCWWGG 2 cut(s) 835, 878
Bst2UI CCWGG 2 cut(s) 204, 940
Bst6I CTCTTC 1 cut(s) 598
BstBAI YACGTR 1 cut(s) 227
BstBI TTCGAA 3 cut(s) 12, 364, 648
BstC8I GCNNGC 3 cut(s) 167, 588, 1217
BstDEI CTNAG 6 cut(s) 117, 249, 797, 1041, 1227, 1233
BstDSI CCRYGG 3 cut(s) 466, 835, 878
BstF5I GGATG 2 cut(s) 244, 388
BstFNI CGCG 1 cut(s) 1017
BstHHI GCGC 1 cut(s) 1019
BstKTI GATC 8 cut(s) 141, 637, 658, 664, 795, 1140, 1261, 1301
BstMAI GTCTC 3 cut(s) 26, 702, 903
BstMBI GATC 8 cut(s) 138, 634, 655, 661, 792, 1137, 1258, 1298
BstMWI GCNNNNNNNGC 8 cut(s) 1063, 1066, 1072, 1075, 1084, 1105, 1114, 1123
BstNI CCWGG 2 cut(s) 204, 940
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 3 cut(s) 202, 347, 938
BstSFI CTRYAG 4 cut(s) 564, 1024, 1245, 1294
BstUI CGCG 1 cut(s) 1017
BstV2I GAAGAC 2 cut(s) 615, 921
BstX2I RGATCY 3 cut(s) 138, 792, 1298
BstYI RGATCY 3 cut(s) 138, 792, 1298
Bsu15I ATCGAT 1 cut(s) 1136
Bsu36I CCTNAGG 1 cut(s) 249
BsuI GTATCC 1 cut(s) 1319
BsuRI GGCC 2 cut(s) 169, 883
BsuTUI ATCGAT 1 cut(s) 1136
BtgI CCRYGG 3 cut(s) 466, 835, 878
BtsCI GGATG 2 cut(s) 244, 388
BtsIMutI CAGTG 1 cut(s) 1171
BveI ACCTGC 1 cut(s) 557
Cac8I GCNNGC 3 cut(s) 167, 588, 1217
CaiI CAGNNNCTG 1 cut(s) 1032
CfoI GCGC 1 cut(s) 1019
Cfr13I GGNCC 3 cut(s) 386, 920, 1198
ClaI ATCGAT 1 cut(s) 1136
CseI GACGC 1 cut(s) 1046
Csp6I GTAC 1 cut(s) 832
CviAII CATG 5 cut(s) 209, 377, 836, 879, 1341
CviQI GTAC 1 cut(s) 832
DdeI CTNAG 6 cut(s) 117, 249, 797, 1041, 1227, 1233
DpnI GATC 8 cut(s) 140, 636, 657, 663, 794, 1139, 1260, 1300
DpnII GATC 8 cut(s) 138, 634, 655, 661, 792, 1137, 1258, 1298
EaeI YGGCCR 1 cut(s) 881
Eam1104I CTCTTC 1 cut(s) 598
EarI CTCTTC 1 cut(s) 598
Eco130I CCWWGG 2 cut(s) 835, 878
Eco24I GRGCYC 1 cut(s) 939
Eco32I GATATC 1 cut(s) 200
Eco47I GGWCC 3 cut(s) 386, 920, 1198
Eco57I CTGAAG 1 cut(s) 213
Eco81I CCTNAGG 1 cut(s) 249
EcoRII CCWGG 2 cut(s) 202, 938
EcoRV GATATC 1 cut(s) 200
EcoT14I CCWWGG 2 cut(s) 835, 878
EcoT38I GRGCYC 1 cut(s) 939
ErhI CCWWGG 2 cut(s) 835, 878
Esp3I CGTCTC 2 cut(s) 702, 903
FaeI CATG 5 cut(s) 212, 380, 839, 882, 1344
FalI AAGNNNNNCTT 2 cut(s) 946, 978
FaqI GGGAC 2 cut(s) 853, 1049
FatI CATG 5 cut(s) 208, 376, 835, 878, 1340
FauI CCCGC 2 cut(s) 524, 1149
FauNDI CATATG 1 cut(s) 1321
FbaI TGATCA 2 cut(s) 634, 661
FokI GGATG 2 cut(s) 231, 395
FriOI GRGCYC 1 cut(s) 939
FspBI CTAG 2 cut(s) 587, 719
GlaI GCGC 1 cut(s) 1018
GsaI CCCAGC 2 cut(s) 755, 1036
GsuI CTGGAG 2 cut(s) 726, 1275
HaeIII GGCC 2 cut(s) 169, 883
HapII CCGG 3 cut(s) 313, 348, 701
HgaI GACGC 1 cut(s) 1046
HhaI GCGC 1 cut(s) 1019
Hin1II CATG 5 cut(s) 212, 380, 839, 882, 1344
Hin6I GCGC 1 cut(s) 1017
HinP1I GCGC 1 cut(s) 1017
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 8 cut(s) 113, 490, 606, 697, 779, 904, 1250, 1285
HpaII CCGG 3 cut(s) 313, 348, 701
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 53, 214
Hpy188I TCNGA 4 cut(s) 118, 485, 696, 1011
Hpy188III TCNNGA 8 cut(s) 110, 313, 638, 659, 684, 890, 908, 1254
Hpy8I GTNNAC 2 cut(s) 53, 214
HpyAV CCTTC 3 cut(s) 188, 320, 1116
HpyCH4IV ACGT 2 cut(s) 226, 1006
HpyCH4V TGCA 6 cut(s) 401, 566, 598, 860, 986, 997
HpyF10VI GCNNNNNNNGC 8 cut(s) 1063, 1066, 1072, 1075, 1084, 1105, 1114, 1123
HpyF3I CTNAG 6 cut(s) 117, 249, 797, 1041, 1227, 1233
HpySE526I ACGT 2 cut(s) 226, 1006
Hsp92II CATG 5 cut(s) 212, 380, 839, 882, 1344
HspAI GCGC 1 cut(s) 1017
Kpn2I TCCGGA 1 cut(s) 312
Ksp22I TGATCA 2 cut(s) 634, 661
Kzo9I GATC 8 cut(s) 138, 634, 655, 661, 792, 1137, 1258, 1298
LmnI GCTCC 2 cut(s) 191, 1193
LweI GCATC 6 cut(s) 220, 388, 973, 1096, 1102, 1126
MaeI CTAG 2 cut(s) 587, 719
MaeII ACGT 2 cut(s) 226, 1006
MaeIII GTNAC 5 cut(s) 99, 125, 178, 334, 1313
MalI GATC 8 cut(s) 140, 636, 657, 663, 794, 1139, 1260, 1300
MbiI CCGCTC 1 cut(s) 1196
MboI GATC 8 cut(s) 138, 634, 655, 661, 792, 1137, 1258, 1298
MboII GAAGA 7 cut(s) 138, 328, 407, 615, 615, 926, 1280
MflI RGATCY 3 cut(s) 138, 792, 1298
MhlI GDGCHC 1 cut(s) 939
MlsI TGGCCA 1 cut(s) 883
MluCI AATT 2 cut(s) 8, 885
MluNI TGGCCA 1 cut(s) 883
MlyI GAGTC 3 cut(s) 615, 788, 1259
MmeI TCCRAC 1 cut(s) 415
MnlI CCTC 7 cut(s) 266, 323, 365, 549, 724, 933, 936
Mox20I TGGCCA 1 cut(s) 883
MroI TCCGGA 1 cut(s) 312
MscI TGGCCA 1 cut(s) 883
MslI CAYNNNNRTG 2 cut(s) 381, 504
Msp20I TGGCCA 1 cut(s) 883
MspI CCGG 3 cut(s) 313, 348, 701
MspR9I CCNGG 3 cut(s) 204, 349, 940
Mva1269I GAATGC 1 cut(s) 1019
MvaI CCWGG 2 cut(s) 204, 940
MvnI CGCG 1 cut(s) 1017
MwoI GCNNNNNNNGC 8 cut(s) 1063, 1066, 1072, 1075, 1084, 1105, 1114, 1123
NciI CCSGG 1 cut(s) 349
NcoI CCATGG 2 cut(s) 835, 878
NdeI CATATG 1 cut(s) 1321
NdeII GATC 8 cut(s) 138, 634, 655, 661, 792, 1137, 1258, 1298
NheI GCTAGC 1 cut(s) 586
NlaIII CATG 5 cut(s) 212, 380, 839, 882, 1344
NlaIV GGNNCC 3 cut(s) 187, 817, 944
NspI RCATGY 1 cut(s) 380
NspV TTCGAA 3 cut(s) 12, 364, 648
PcsI WCGNNNNNNNCGW 1 cut(s) 615
PctI GAATGC 1 cut(s) 1019
PfeI GAWTC 5 cut(s) 113, 490, 697, 904, 1285
PleI GAGTC 3 cut(s) 614, 787, 1258
PpsI GAGTC 3 cut(s) 614, 787, 1258
Ppu21I YACGTR 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 202, 938
PspFI CCCAGC 2 cut(s) 751, 1032
PspGI CCWGG 2 cut(s) 202, 938
PspN4I GGNNCC 3 cut(s) 187, 817, 944
PspPI GGNCC 3 cut(s) 386, 920, 1198
PstI CTGCAG 1 cut(s) 568
PstNI CAGNNNCTG 1 cut(s) 1032
PsuI RGATCY 3 cut(s) 138, 792, 1298
RsaI GTAC 1 cut(s) 833
RsaNI GTAC 1 cut(s) 832
RseI CAYNNNNRTG 2 cut(s) 381, 504
Sau3AI GATC 8 cut(s) 138, 634, 655, 661, 792, 1137, 1258, 1298
Sau96I GGNCC 3 cut(s) 386, 920, 1198
SchI GAGTC 3 cut(s) 615, 788, 1259
ScrFI CCNGG 3 cut(s) 204, 349, 940
SduI GDGCHC 1 cut(s) 939
SfaNI GCATC 6 cut(s) 220, 388, 973, 1096, 1102, 1126
SfcI CTRYAG 4 cut(s) 564, 1024, 1245, 1294
SfuI TTCGAA 3 cut(s) 12, 364, 648
SinI GGWCC 3 cut(s) 386, 920, 1198
SmiMI CAYNNNNRTG 2 cut(s) 381, 504
Sse9I AATT 2 cut(s) 8, 885
SsiI CCGC 4 cut(s) 531, 1015, 1142, 1196
SspMI CTAG 2 cut(s) 587, 719
StyD4I CCNGG 3 cut(s) 202, 347, 938
StyI CCWWGG 2 cut(s) 835, 878
TaiI ACGT 2 cut(s) 229, 1009
TaqI TCGA 7 cut(s) 12, 364, 504, 648, 907, 1120, 1136
TasI AATT 2 cut(s) 8, 885
TauI GCSGC 1 cut(s) 1017
TfiI GAWTC 5 cut(s) 113, 490, 697, 904, 1285
TscAI CASTG 1 cut(s) 1171
TspDTI ATGAA 3 cut(s) 144, 689, 1280
TspGWI ACGGA 2 cut(s) 108, 933
TspRI CASTG 1 cut(s) 1171
VpaK11BI GGWCC 3 cut(s) 386, 920, 1198
XapI RAATTY 1 cut(s) 8
XceI RCATGY 1 cut(s) 380
XspI CTAG 2 cut(s) 587, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.