Rorug04G0350500

NHL domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003391
Physical Location & Seq
Reverse (-)
52225455 .. 52227585
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0350500.1

Sequence Viewer

Length: 1398 bp
ATGACTTCATCCACTCTACTACTGTTTTTCTGGATTGTTCTTGTCACATTTTCCATAACTTCGGTTGAGGCAAGAAAGCACCAAACCAAGAAGAGCAAACCCCAAAAACATCAAAAAGGTGGTAATAACACTCATGATTCACATTTGCCTCGTCCTGCTCCAACTCCAGTTCCTCAGTATGGTTCCTTTTCTGTCTTGTCCTTTGGAGCCAAGGGTGATGGTGTCTCCGACGATTCTAAGGCGCTCATAGCGGCATGGAAAGCTGCCTGCAAAGTGAATGGGGCTAGTGTAGAAATCCCTTCAGGATTCAAGTTCCTCATCAAGCCAATAACTCTTCAAGGCCCTTGTATGCCTGACGTTGTTCTTCAGATAGATGGAACTCTAATGGCTCCTCCAAAAGTTAGCTCATGGTCAAAGTCCAGTTTGTTTCAGTGGATAAACTTCAAATGGGTTCATAACTTCACCATCCGGGGCACCGGAGTTGTCGATGGCCAGGGCTCTGACTGGTGGACTAGTAGTTCCTCTTCCTCTTCAATCTATAATTACTTGCAGAAGAAGTCCAACAAGCATATGCCGAATTTCAAACCCACTGCTTTGAGATTCTATTCAAGCTATGACGTGACAGTACGAGATATCAGAATCATAAACAGCCCTCAGTGCCATTTGAAATTTGACAGCTCTGGAGGGATCATAGTTGACAACATTACCATATCTTCACCAGAGAATAGTCCAAATACTGATGGAATTCACTTGCAGAACACCCAAAACGTCGAAATTCATCATTCTACTATTGGTTGTGGAGATGACTGTGTATCCATACAAACTGGCTGCTCCAATGTTCATGTTCATCATATCAATTGTGGCCCTGGCCATGGCATAAGTCTAGGAGGACTTGGAAAAGACAGAAGTGTTGCTTGTGTCTCAAATATAATTGTCAATGACATTACATTTCAAAACACTCTATCTGGAGTTAGGATCAAAACATGGCAGGGAGGCATTGGGTCTGTGAAGAATGTATCTTTTTCCAATATCCAAGTTTCGAATGTGAAGGTTCCGATAATGATAGACCAATACTACTGTGACAAGAAGTCCTGCAAGAACCAAACAGGAGCTGTAGCAATCTCAGGTGTGAAATATGACCAAATAATTGGGACTTATTCGGTGCAGCCTGTTCATCTTGCTTGCAGCAGCGACATTCCATGCACAGATGTTGACCTCATTGATATTCAGCTCAAGCCCTCAGCAGGGTACCAGAGATTTCAACACGGTTTGTGTTGGAACTCTTATGGCAAGTCACAAGCTCCTTTGGTCCCTTCAAGCATGGACTACTGCTTGAGAAGAGATGAAAATCCAGTCGAGAGAATAGCGAAGTCACATGATCGTCAGCATCTGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

465

Amino Acids

50.91

Weight (kDa)

8.91

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 95 - 413 1e-80 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1248
AccB1I GGYRCC 2 cut(s) 473, 1248
AciI CCGC 1 cut(s) 251
AclWI GGATC 2 cut(s) 695, 983
AcoI YGGCCR 2 cut(s) 490, 868
AcsI RAATTY 4 cut(s) 577, 668, 744, 774
AcuI CTGAAG 2 cut(s) 285, 350
AfaI GTAC 2 cut(s) 627, 1250
AfiI CCNNNNNNNGG 4 cut(s) 179, 872, 1244, 1245
AhdI GACNNNNNGTC 1 cut(s) 1087
AhlI ACTAGT 1 cut(s) 512
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 2 cut(s) 492, 865
AloI GAACNNNNNNTCC 2 cut(s) 502, 534
AluBI AGCT 7 cut(s) 263, 405, 612, 678, 1112, 1231, 1301
AluI AGCT 7 cut(s) 263, 405, 612, 678, 1112, 1231, 1301
Alw26I GTCTC 2 cut(s) 229, 925
AlwI GGATC 2 cut(s) 695, 983
AlwNI CAGNNNCTG 2 cut(s) 1112, 1390
AoxI GGCC 4 cut(s) 340, 490, 862, 868
ApeKI GCWGC 5 cut(s) 263, 828, 1165, 1185, 1188
ApoI RAATTY 4 cut(s) 577, 668, 744, 774
Asp718I GGTACC 1 cut(s) 1248
AspLEI GCGC 1 cut(s) 244
AspS9I GGNCC 3 cut(s) 341, 863, 1309
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 3 cut(s) 227, 454, 708
AsuII TTCGAA 1 cut(s) 1040
AvaII GGWCC 1 cut(s) 1309
BaeGI GKGCMC 1 cut(s) 476
BalI TGGCCA 2 cut(s) 492, 870
BanI GGYRCC 2 cut(s) 473, 1248
BanII GRGCYC 1 cut(s) 500
BarI GAAGNNNNNNTAC 2 cut(s) 508, 540
BbvCI CCTCAGC 1 cut(s) 1240
BbvI GCAGC 5 cut(s) 250, 815, 1177, 1197, 1200
BccI CCATC 5 cut(s) 212, 368, 473, 482, 734
BciT130I CCWGG 2 cut(s) 494, 867
BciVI GTATCC 1 cut(s) 823
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 2 cut(s) 229, 925
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 4 cut(s) 285, 513, 884, 1396
BfmI CTRYAG 1 cut(s) 1113
BfoI RGCGCY 1 cut(s) 245
BfuI GTATCC 1 cut(s) 823
BisI GCNGC 6 cut(s) 252, 264, 829, 1166, 1186, 1189
BlsI GCNGC 6 cut(s) 253, 265, 830, 1167, 1187, 1190
Bme1390I CCNGG 3 cut(s) 470, 494, 867
Bme18I GGWCC 1 cut(s) 1309
BmeRI GACNNNNNGTC 1 cut(s) 1087
BmgBI CACGTC 1 cut(s) 619
BmgT120I GGNCC 3 cut(s) 341, 863, 1309
BmiI GGNNCC 7 cut(s) 184, 208, 390, 475, 1053, 1250, 1311
BmrFI CCNGG 3 cut(s) 470, 494, 867
BpmI CTGGAG 3 cut(s) 150, 702, 987
Bpu10I CCTNAGC 1 cut(s) 1240
Bpu14I TTCGAA 1 cut(s) 1040
BpuEI CTTGAG 2 cut(s) 1217, 1354
BpuMI CCSGG 1 cut(s) 470
BsaBI GATNNNNATC 1 cut(s) 1347
BsaJI CCNNGG 5 cut(s) 210, 469, 493, 865, 871
BsaWI WCCGGW 1 cut(s) 476
Bsc4I CCNNNNNNNGG 4 cut(s) 179, 872, 1244, 1245
Bse1I ACTGG 5 cut(s) 167, 420, 509, 829, 1352
Bse8I GATNNNNATC 1 cut(s) 1347
BseBI CCWGG 2 cut(s) 494, 867
BseDI CCNNGG 5 cut(s) 210, 469, 493, 865, 871
BseGI GGATG 2 cut(s) 8, 465
BseJI GATNNNNATC 1 cut(s) 1347
BseLI CCNNNNNNNGG 4 cut(s) 179, 872, 1244, 1245
BseMII CTCAG 4 cut(s) 188, 668, 1137, 1254
BseNI ACTGG 5 cut(s) 167, 420, 509, 829, 1352
BseRI GAGGAG 1 cut(s) 381
BseSI GKGCMC 1 cut(s) 476
BseXI GCAGC 5 cut(s) 250, 815, 1177, 1197, 1200
BsgI GTGCAG 1 cut(s) 1184
BshFI GGCC 4 cut(s) 342, 492, 864, 870
BshNI GGYRCC 2 cut(s) 473, 1248
BsiSI CCGG 2 cut(s) 469, 477
BslFI GGGAC 2 cut(s) 1165, 1295
BslI CCNNNNNNNGG 4 cut(s) 179, 872, 1244, 1245
BsmAI GTCTC 2 cut(s) 229, 925
BsmFI GGGAC 2 cut(s) 1165, 1295
BsnI GGCC 4 cut(s) 342, 492, 864, 870
Bsp119I TTCGAA 1 cut(s) 1040
Bsp1286I GDGCHC 2 cut(s) 476, 500
Bsp143I GATC 3 cut(s) 687, 975, 1378
Bsp19I CCATGG 1 cut(s) 871
BspACI CCGC 1 cut(s) 251
BspANI GGCC 4 cut(s) 342, 492, 864, 870
BspCNI CTCAG 4 cut(s) 187, 667, 1136, 1253
BspHI TCATGA 1 cut(s) 133
BspLI GGNNCC 7 cut(s) 184, 208, 390, 475, 1053, 1250, 1311
BspPI GGATC 2 cut(s) 695, 983
BspQI GCTCTTC 1 cut(s) 86
BspT104I TTCGAA 1 cut(s) 1040
BspT107I GGYRCC 2 cut(s) 473, 1248
BsrI ACTGG 5 cut(s) 167, 420, 509, 829, 1352
BssECI CCNNGG 5 cut(s) 210, 469, 493, 865, 871
BssMI GATC 3 cut(s) 687, 975, 1378
BssT1I CCWWGG 2 cut(s) 210, 871
Bst2UI CCWGG 2 cut(s) 494, 867
Bst4CI ACNGT 5 cut(s) 24, 625, 809, 1079, 1268
Bst6I CTCTTC 5 cut(s) 86, 339, 529, 535, 1333
BstBI TTCGAA 1 cut(s) 1040
BstC8I GCNNGC 2 cut(s) 268, 1183
BstDEI CTNAG 5 cut(s) 174, 237, 654, 1123, 1240
BstDSI CCRYGG 1 cut(s) 871
BstF5I GGATG 2 cut(s) 8, 465
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 3 cut(s) 690, 978, 1381
BstMAI GTCTC 2 cut(s) 229, 925
BstMBI GATC 3 cut(s) 687, 975, 1378
BstMWI GCNNNNNNNGC 3 cut(s) 248, 260, 657
BstNI CCWGG 2 cut(s) 494, 867
BstSCI CCNGG 3 cut(s) 468, 492, 865
BstSFI CTRYAG 1 cut(s) 1113
BstSLI GKGCMC 1 cut(s) 476
BstV1I GCAGC 5 cut(s) 250, 815, 1177, 1197, 1200
BstXI CCANNNNNNTGG 1 cut(s) 1148
BsuI GTATCC 1 cut(s) 823
BsuRI GGCC 4 cut(s) 342, 492, 864, 870
BtgI CCRYGG 1 cut(s) 871
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 2 cut(s) 8, 465
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 3 cut(s) 437, 588, 662
Cac8I GCNNGC 2 cut(s) 268, 1183
CaiI CAGNNNCTG 2 cut(s) 1112, 1390
CciI TCATGA 1 cut(s) 133
CfoI GCGC 1 cut(s) 244
Cfr13I GGNCC 3 cut(s) 341, 863, 1309
Csp6I GTAC 2 cut(s) 626, 1249
CviAII CATG 9 cut(s) 134, 255, 408, 842, 872, 984, 1200, 1321, 1376
CviQI GTAC 2 cut(s) 626, 1249
DdeI CTNAG 5 cut(s) 174, 237, 654, 1123, 1240
DpnI GATC 3 cut(s) 689, 977, 1380
DpnII GATC 3 cut(s) 687, 975, 1378
DriI GACNNNNNGTC 1 cut(s) 1087
EaeI YGGCCR 2 cut(s) 490, 868
Eam1104I CTCTTC 5 cut(s) 86, 339, 529, 535, 1333
Eam1105I GACNNNNNGTC 1 cut(s) 1087
EarI CTCTTC 5 cut(s) 86, 339, 529, 535, 1333
Eco130I CCWWGG 2 cut(s) 210, 871
Eco24I GRGCYC 1 cut(s) 500
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 1309
Eco57I CTGAAG 2 cut(s) 285, 350
EcoO109I RGGNCCY 1 cut(s) 341
EcoRI GAATTC 1 cut(s) 744
EcoRII CCWGG 2 cut(s) 492, 865
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 2 cut(s) 210, 871
EcoT38I GRGCYC 1 cut(s) 500
ErhI CCWWGG 2 cut(s) 210, 871
FaeI CATG 9 cut(s) 137, 258, 411, 845, 875, 987, 1203, 1324, 1379
FalI AAGNNNNNCTT 2 cut(s) 898, 930
FaqI GGGAC 2 cut(s) 1165, 1295
FatI CATG 9 cut(s) 133, 254, 407, 841, 871, 983, 1199, 1320, 1375
FauNDI CATATG 1 cut(s) 570
Fnu4HI GCNGC 6 cut(s) 252, 264, 829, 1166, 1186, 1189
FokI GGATG 1 cut(s) 452
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 6 cut(s) 252, 264, 829, 1166, 1186, 1189
FspBI CTAG 4 cut(s) 285, 513, 884, 1396
GlaI GCGC 1 cut(s) 243
GluI GCNGC 6 cut(s) 252, 264, 829, 1166, 1186, 1189
GsuI CTGGAG 3 cut(s) 150, 702, 987
HaeII RGCGCY 1 cut(s) 245
HaeIII GGCC 4 cut(s) 342, 492, 864, 870
HapII CCGG 2 cut(s) 469, 477
HhaI GCGC 1 cut(s) 244
Hin1II CATG 9 cut(s) 137, 258, 411, 845, 875, 987, 1203, 1324, 1379
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HincII GTYRAC 2 cut(s) 697, 1213
HindII GTYRAC 2 cut(s) 697, 1213
HinfI GANTC 5 cut(s) 137, 233, 306, 600, 639
HpaII CCGG 2 cut(s) 469, 477
HphI GGTGA 3 cut(s) 227, 454, 708
Hpy166II GTNNAC 3 cut(s) 510, 697, 1213
Hpy188I TCNGA 5 cut(s) 229, 369, 502, 638, 1056
Hpy188III TCNNGA 6 cut(s) 31, 134, 303, 681, 966, 1357
Hpy8I GTNNAC 3 cut(s) 510, 697, 1213
Hpy99I CGWCG 2 cut(s) 233, 773
HpyAV CCTTC 3 cut(s) 309, 1042, 1323
HpyCH4III ACNGT 5 cut(s) 24, 625, 809, 1079, 1268
HpyCH4IV ACGT 3 cut(s) 357, 618, 768
HpyCH4V TGCA 7 cut(s) 270, 550, 754, 1095, 1165, 1185, 1203
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 260, 657
HpyF3I CTNAG 5 cut(s) 174, 237, 654, 1123, 1240
HpySE526I ACGT 3 cut(s) 357, 618, 768
Hsp92II CATG 9 cut(s) 137, 258, 411, 845, 875, 987, 1203, 1324, 1379
HspAI GCGC 1 cut(s) 242
KpnI GGTACC 1 cut(s) 1252
Kzo9I GATC 3 cut(s) 687, 975, 1378
LguI GCTCTTC 1 cut(s) 86
LmnI GCTCC 6 cut(s) 163, 206, 394, 836, 1109, 1306
Lsp1109I GCAGC 5 cut(s) 250, 815, 1177, 1197, 1200
MaeI CTAG 4 cut(s) 285, 513, 884, 1396
MaeII ACGT 3 cut(s) 357, 618, 768
MaeIII GTNAC 5 cut(s) 43, 619, 1079, 1293, 1371
MalI GATC 3 cut(s) 689, 977, 1380
MboI GATC 3 cut(s) 687, 975, 1378
MboII GAAGA 9 cut(s) 103, 326, 356, 516, 522, 565, 705, 1021, 1350
MfeI CAATTG 1 cut(s) 856
MhlI GDGCHC 2 cut(s) 476, 500
MlsI TGGCCA 2 cut(s) 492, 870
MluCI AATT 8 cut(s) 541, 577, 668, 744, 774, 856, 930, 1146
MluNI TGGCCA 2 cut(s) 492, 870
MmeI TCCRAC 4 cut(s) 185, 252, 585, 1256
Mox20I TGGCCA 2 cut(s) 492, 870
MscI TGGCCA 2 cut(s) 492, 870
Msp20I TGGCCA 2 cut(s) 492, 870
MspI CCGG 2 cut(s) 469, 477
MspR9I CCNGG 3 cut(s) 470, 494, 867
MunI CAATTG 1 cut(s) 856
MvaI CCWGG 2 cut(s) 494, 867
MwoI GCNNNNNNNGC 3 cut(s) 248, 260, 657
NciI CCSGG 1 cut(s) 470
NcoI CCATGG 1 cut(s) 871
NdeI CATATG 1 cut(s) 570
NdeII GATC 3 cut(s) 687, 975, 1378
NlaIII CATG 9 cut(s) 137, 258, 411, 845, 875, 987, 1203, 1324, 1379
NlaIV GGNNCC 7 cut(s) 184, 208, 390, 475, 1053, 1250, 1311
NmuCI GTSAC 5 cut(s) 43, 619, 1079, 1293, 1371
NspV TTCGAA 1 cut(s) 1040
PagI TCATGA 1 cut(s) 133
PciSI GCTCTTC 1 cut(s) 86
PfeI GAWTC 5 cut(s) 137, 233, 306, 600, 639
PkrI GCNGC 6 cut(s) 253, 265, 830, 1167, 1187, 1190
Psp6I CCWGG 2 cut(s) 492, 865
PspGI CCWGG 2 cut(s) 492, 865
PspN4I GGNNCC 7 cut(s) 184, 208, 390, 475, 1053, 1250, 1311
PspPI GGNCC 3 cut(s) 341, 863, 1309
PstNI CAGNNNCTG 2 cut(s) 1112, 1390
RsaI GTAC 2 cut(s) 627, 1250
RsaNI GTAC 2 cut(s) 626, 1249
SapI GCTCTTC 1 cut(s) 86
SatI GCNGC 6 cut(s) 252, 264, 829, 1166, 1186, 1189
Sau3AI GATC 3 cut(s) 687, 975, 1378
Sau96I GGNCC 3 cut(s) 341, 863, 1309
ScrFI CCNGG 3 cut(s) 470, 494, 867
SduI GDGCHC 2 cut(s) 476, 500
SfcI CTRYAG 1 cut(s) 1113
SfuI TTCGAA 1 cut(s) 1040
SinI GGWCC 1 cut(s) 1309
SmlI CTYRAG 2 cut(s) 1232, 1333
SmoI CTYRAG 2 cut(s) 1232, 1333
SpeI ACTAGT 1 cut(s) 512
Sse9I AATT 8 cut(s) 541, 577, 668, 744, 774, 856, 930, 1146
SsiI CCGC 1 cut(s) 251
SspMI CTAG 4 cut(s) 285, 513, 884, 1396
StyD4I CCNGG 3 cut(s) 468, 492, 865
StyI CCWWGG 2 cut(s) 210, 871
TaaI ACNGT 5 cut(s) 24, 625, 809, 1079, 1268
TaiI ACGT 3 cut(s) 360, 621, 771
TaqI TCGA 4 cut(s) 486, 771, 1040, 1356
TasI AATT 8 cut(s) 541, 577, 668, 744, 774, 856, 930, 1146
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 5 cut(s) 137, 233, 306, 600, 639
TscAI CASTG 3 cut(s) 437, 595, 662
TseFI GTSAC 5 cut(s) 43, 619, 1079, 1293, 1371
TseI GCWGC 5 cut(s) 263, 828, 1165, 1185, 1188
Tsp45I GTSAC 5 cut(s) 43, 619, 1079, 1293, 1371
TspDTI ATGAA 6 cut(s) 443, 767, 830, 836, 1163, 1359
TspRI CASTG 3 cut(s) 437, 595, 662
VpaK11BI GGWCC 1 cut(s) 1309
XapI RAATTY 4 cut(s) 577, 668, 744, 774
XspI CTAG 4 cut(s) 285, 513, 884, 1396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.