RchiOBHm_Chr4g0444151

NHL domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003391
Physical Location & Seq
Forward (+)
64994754 .. 64998893
4140 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41185

Sequence Viewer

Length: 1455 bp
ATGGGTCGGACAGTTTTGGTTCTGGGTCTTGTTCTTTTGGTTCTGATCGGAGGTGCCTCATCGGCTCCGATGAGTCCCACATCAAAGATTGTAAGTGGGTATTTCTCAAATTTGGCGTCTGTGTTTATGAAATGGCTCTGGTCACTGAAAGCAACAACCAAGACAGCGGTTTCGGGCCGTCCGATGGTGAAGTTTGAGAGTGGGTTTACAGTGGAGACAGTGTTTGATGGGAGCAAGCTTGGTATTGAGCCTCACACTGTTGAGGTTTTGCCAAATGGGGAGCTTTTGATTTTGGACTCTGCTAATAGTAACATTTATAGAATGTCTTCGTCTCTATCTATATATAGCAGACCAAAGCTGGTTGCAGGGTCCCCTGAAGGCAATGTTGGACATATAGATGGAAAGCCCAGAGAGGCAAGAATGAACCACCCAAAGGGGCTTACAGTTGATGACAGGGGAAACATTTATATTGCAGACACAAGAAATATGGCAATCAGGAAGATTAGTGATGCTGGGGTCACAACAATTGCAGGAGGGAAATTGGGCCGTGGGGGAAGCCATGTGGATGGACCAAGTGAAGATGCAAAATTTTCCAACGACTTTGATGTGGTTTACATTGGAAGCAGCTGCTCCTTACTTGTCATAGACAGAGGAAATCAAGCTATCAGGGAGATCCAACTCCATTTTGATGATTGTGCTTACCAATATGGAAGTGGTTTCCCACTTGGGATTGCGATGCTTCTGGGGGCAGGCTTTTTTGGTTACATGTTAGCATTACTGCAACGTAGAGTGGGTACAATTGTCTCTTCCCAAAATGATGAAGACCTAACCAAGCCAAGAATGCCGTCAAGTCCATACAAAAAGCCTCTGAAATCAGTCATGCCACCACTGATTCCAACTGAAGATGAGCAGGAGAAGCAAGAAGAAGGCTTCTTTGGATCACTTGGAAAGCTTTTAGTCAATGCCTGGGCATCTATTTTGGAAATTCTTGGGAAAAAGCATTCAAACTATCAAGAACAAAGCCAGCAGCAACAGCAGCACAAGCATTCAAATACTTGGCCTGTGCAAGATAGTTATGTGATAGCAGATGAGGATGAGCCGCCTACAATTGAGACCCGAACCCCTACCCCACGCAAAACCTATGCGTTTATGGCCAAGGATGCTGAAACAATGCAACAGTTGCGACAAAGCCGAGTTTTCTACAATGGGTGGGGTGATCATCAACAGCAGCCAAAACAGCAGCAGCATTATCATCGTCACCAGTCATCCACTCCACAAACTTTCTATGAGCAGAGCTGTGATCAAACCACTGAGATTGTGTTTGGGGCAGTCCAGGAGCAGGATGGGAAGGGTGAAGCTGTGGTCATTAAGCCTATTGATTACGGAGATCCCATGCTTGGTCATCGCAGTGTTAGCTACCGATTCAATTCAATGGGTTATCCCCGTGGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

484

Amino Acids

53.56

Weight (kDa)

6.68

Isoelectric Point (pI)

49.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 53
AciI CCGC 2 cut(s) 167, 1100
AclWI GGATC 3 cut(s) 667, 946, 1382
AcoI YGGCCR 1 cut(s) 1152
AcsI RAATTY 3 cut(s) 109, 587, 984
AcuI CTGAAG 2 cut(s) 396, 921
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 1 cut(s) 796
AfiI CCNNNNNNNGG 4 cut(s) 184, 433, 1339, 1397
AflIII ACRYGT 1 cut(s) 765
AgsI TTSAA 4 cut(s) 1005, 1050, 1426, 1431
AjnI CCWGG 2 cut(s) 965, 1332
AjuI GAANNNNNNNTTGG 4 cut(s) 369, 401, 918, 950
AluBI AGCT 9 cut(s) 238, 283, 358, 627, 662, 952, 1296, 1358, 1416
AluI AGCT 9 cut(s) 238, 283, 358, 627, 662, 952, 1296, 1358, 1416
Alw26I GTCTC 4 cut(s) 209, 336, 808, 1106
AlwI GGATC 3 cut(s) 667, 946, 1382
AoxI GGCC 4 cut(s) 175, 544, 1058, 1152
ApeKI GCWGC 7 cut(s) 624, 627, 1027, 1036, 1228, 1240, 1243
ApoI RAATTY 3 cut(s) 109, 587, 984
ArsI GACNNNNNNTTYG 4 cut(s) 101, 133, 154, 186
Asp700I GAANNNNTTC 1 cut(s) 325
AspS9I GGNCC 4 cut(s) 175, 369, 544, 569
AsuHPI GGTGA 4 cut(s) 199, 1226, 1250, 1364
AvaII GGWCC 2 cut(s) 369, 569
BaeI ACNNNNGTAYC 2 cut(s) 786, 819
BalI TGGCCA 1 cut(s) 1154
BanI GGYRCC 1 cut(s) 53
BbsI GAAGAC 2 cut(s) 318, 828
BbvI GCAGC 7 cut(s) 614, 636, 1039, 1048, 1240, 1252, 1255
BccI CCATC 5 cut(s) 178, 221, 392, 560, 1337
BceAI ACGGC 3 cut(s) 162, 531, 829
BcgI CGANNNNNNTGC 2 cut(s) 1235, 1269
BciT130I CCWGG 2 cut(s) 967, 1334
BclI TGATCA 2 cut(s) 1216, 1300
BcoDI GTCTC 4 cut(s) 209, 336, 808, 1106
BglI GCCNNNNNGGC 1 cut(s) 62
BisI GCNGC 8 cut(s) 625, 628, 1028, 1037, 1100, 1229, 1241, 1244
BlsI GCNGC 8 cut(s) 626, 629, 1029, 1038, 1101, 1230, 1242, 1245
Bme1390I CCNGG 2 cut(s) 967, 1334
Bme18I GGWCC 2 cut(s) 369, 569
BmgT120I GGNCC 4 cut(s) 175, 369, 544, 569
BmiI GGNNCC 4 cut(s) 55, 66, 370, 371
BmrFI CCNGG 2 cut(s) 967, 1334
BmsI GCATC 5 cut(s) 499, 571, 726, 980, 1150
BpiI GAAGAC 2 cut(s) 318, 828
BsaHI GRCGYC 1 cut(s) 116
BsaI GGTCTC 1 cut(s) 1106
BsaJI CCNNGG 4 cut(s) 547, 966, 1155, 1444
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 433, 1339, 1397
Bse1I ACTGG 1 cut(s) 1261
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 2 cut(s) 967, 1334
BseDI CCNNGG 4 cut(s) 547, 966, 1155, 1444
BseGI GGATG 5 cut(s) 571, 1099, 1165, 1265, 1348
BseLI CCNNNNNNNGG 4 cut(s) 184, 433, 1339, 1397
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 1 cut(s) 1302
BseNI ACTGG 1 cut(s) 1261
BseXI GCAGC 7 cut(s) 614, 636, 1039, 1048, 1240, 1252, 1255
BseYI CCCAGC 1 cut(s) 512
BshFI GGCC 4 cut(s) 177, 546, 1060, 1154
BshNI GGYRCC 1 cut(s) 53
BslFI GGGAC 2 cut(s) 60, 355
BslI CCNNNNNNNGG 4 cut(s) 184, 433, 1339, 1397
BsmAI GTCTC 4 cut(s) 209, 336, 808, 1106
BsmBI CGTCTC 1 cut(s) 336
BsmFI GGGAC 2 cut(s) 60, 355
BsmI GAATGC 3 cut(s) 846, 1000, 1045
BsnI GGCC 4 cut(s) 177, 546, 1060, 1154
Bso31I GGTCTC 1 cut(s) 1106
Bsp143I GATC 6 cut(s) 45, 672, 938, 1216, 1300, 1387
BspACI CCGC 2 cut(s) 167, 1100
BspANI GGCC 4 cut(s) 177, 546, 1060, 1154
BspCNI CTCAG 1 cut(s) 1303
BspLI GGNNCC 4 cut(s) 55, 66, 370, 371
BspPI GGATC 3 cut(s) 667, 946, 1382
BspT107I GGYRCC 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 1106
BsrDI GCAATG 1 cut(s) 388
BsrI ACTGG 1 cut(s) 1261
BssECI CCNNGG 4 cut(s) 547, 966, 1155, 1444
BssMI GATC 6 cut(s) 45, 672, 938, 1216, 1300, 1387
BssNI GRCGYC 1 cut(s) 116
BssT1I CCWWGG 1 cut(s) 1155
Bst2UI CCWGG 2 cut(s) 967, 1334
Bst4CI ACNGT 6 cut(s) 13, 211, 220, 259, 445, 1179
Bst6I CTCTTC 1 cut(s) 811
BstACI GRCGYC 1 cut(s) 116
BstAPI GCANNNNNTGC 1 cut(s) 1180
BstC8I GCNNGC 3 cut(s) 236, 751, 1025
BstDEI CTNAG 1 cut(s) 1311
BstDSI CCRYGG 2 cut(s) 547, 1444
BstF5I GGATG 5 cut(s) 571, 1099, 1165, 1265, 1348
BstKTI GATC 6 cut(s) 48, 675, 941, 1219, 1303, 1390
BstMAI GTCTC 4 cut(s) 209, 336, 808, 1106
BstMBI GATC 6 cut(s) 45, 672, 938, 1216, 1300, 1387
BstNI CCWGG 2 cut(s) 967, 1334
BstNSI RCATGY 1 cut(s) 769
BstSCI CCNGG 2 cut(s) 965, 1332
BstV1I GCAGC 7 cut(s) 614, 636, 1039, 1048, 1240, 1252, 1255
BstV2I GAAGAC 2 cut(s) 318, 828
BstX2I RGATCY 2 cut(s) 672, 1387
BstXI CCANNNNNNTGG 1 cut(s) 566
BstYI RGATCY 2 cut(s) 672, 1387
BsuRI GGCC 4 cut(s) 177, 546, 1060, 1154
BtgI CCRYGG 2 cut(s) 547, 1444
BtgZI GCGATG 2 cut(s) 749, 1388
BtsCI GGATG 5 cut(s) 571, 1099, 1165, 1265, 1348
BtsI GCAGTG 1 cut(s) 1414
BtsIMutI CAGTG 7 cut(s) 143, 216, 225, 255, 887, 1308, 1414
Cac8I GCNNGC 3 cut(s) 236, 751, 1025
Cfr13I GGNCC 4 cut(s) 175, 369, 544, 569
CseI GACGC 1 cut(s) 105
Csp6I GTAC 1 cut(s) 795
CspCI CAANNNNNGTGG 2 cut(s) 1297, 1332
CviAII CATG 4 cut(s) 560, 766, 880, 1393
CviQI GTAC 1 cut(s) 795
DdeI CTNAG 1 cut(s) 1311
DpnI GATC 6 cut(s) 47, 674, 940, 1218, 1302, 1389
DpnII GATC 6 cut(s) 45, 672, 938, 1216, 1300, 1387
EaeI YGGCCR 1 cut(s) 1152
Eam1104I CTCTTC 1 cut(s) 811
EarI CTCTTC 1 cut(s) 811
Eco130I CCWWGG 1 cut(s) 1155
Eco31I GGTCTC 1 cut(s) 1106
Eco47I GGWCC 2 cut(s) 369, 569
Eco57I CTGAAG 2 cut(s) 396, 921
EcoO109I RGGNCCY 1 cut(s) 369
EcoRII CCWGG 2 cut(s) 965, 1332
EcoT14I CCWWGG 1 cut(s) 1155
ErhI CCWWGG 1 cut(s) 1155
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 4 cut(s) 563, 769, 883, 1396
FaqI GGGAC 2 cut(s) 60, 355
FatI CATG 4 cut(s) 559, 765, 879, 1392
FbaI TGATCA 2 cut(s) 1216, 1300
Fnu4HI GCNGC 8 cut(s) 625, 628, 1028, 1037, 1100, 1229, 1241, 1244
FokI GGATG 5 cut(s) 578, 1106, 1172, 1252, 1355
Fsp4HI GCNGC 8 cut(s) 625, 628, 1028, 1037, 1100, 1229, 1241, 1244
GluI GCNGC 8 cut(s) 625, 628, 1028, 1037, 1100, 1229, 1241, 1244
GsaI CCCAGC 1 cut(s) 516
HaeIII GGCC 4 cut(s) 177, 546, 1060, 1154
HgaI GACGC 1 cut(s) 105
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 4 cut(s) 563, 769, 883, 1396
HindIII AAGCTT 2 cut(s) 236, 950
HinfI GANTC 4 cut(s) 73, 296, 892, 1422
HphI GGTGA 4 cut(s) 199, 1226, 1250, 1364
Hpy166II GTNNAC 2 cut(s) 207, 613
Hpy188I TCNGA 6 cut(s) 9, 45, 50, 69, 183, 870
Hpy188III TCNNGA 2 cut(s) 496, 1013
Hpy8I GTNNAC 2 cut(s) 207, 613
HpyAV CCTTC 3 cut(s) 371, 920, 1342
HpyCH4III ACNGT 6 cut(s) 13, 211, 220, 259, 445, 1179
HpyCH4IV ACGT 1 cut(s) 784
HpyCH4V TGCA 7 cut(s) 365, 473, 530, 584, 781, 1066, 1174
HpyF3I CTNAG 1 cut(s) 1311
HpySE526I ACGT 1 cut(s) 784
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 4 cut(s) 563, 769, 883, 1396
KflI GGGWCCC 1 cut(s) 369
Ksp22I TGATCA 2 cut(s) 1216, 1300
Kzo9I GATC 6 cut(s) 45, 672, 938, 1216, 1300, 1387
LmnI GCTCC 5 cut(s) 70, 231, 280, 635, 1336
Lsp1109I GCAGC 7 cut(s) 614, 636, 1039, 1048, 1240, 1252, 1255
LweI GCATC 5 cut(s) 499, 571, 726, 980, 1150
MaeII ACGT 1 cut(s) 784
MaeIII GTNAC 5 cut(s) 141, 308, 517, 761, 1256
MalI GATC 6 cut(s) 47, 674, 940, 1218, 1302, 1389
MboI GATC 6 cut(s) 45, 672, 938, 1216, 1300, 1387
MboII GAAGA 7 cut(s) 318, 511, 590, 798, 833, 914, 935
MfeI CAATTG 3 cut(s) 525, 798, 1107
MflI RGATCY 2 cut(s) 672, 1387
MlsI TGGCCA 1 cut(s) 1154
MluCI AATT 8 cut(s) 109, 525, 539, 587, 798, 984, 1107, 1426
MluNI TGGCCA 1 cut(s) 1154
MlyI GAGTC 2 cut(s) 82, 290
MmeI TCCRAC 4 cut(s) 367, 618, 700, 920
MnlI CCTC 9 cut(s) 44, 67, 256, 261, 406, 527, 644, 876, 1084
Mox20I TGGCCA 1 cut(s) 1154
MroXI GAANNNNTTC 1 cut(s) 325
MscI TGGCCA 1 cut(s) 1154
MseI TTAA 1 cut(s) 1368
MslI CAYNNNNRTG 4 cut(s) 396, 564, 687, 1407
Msp20I TGGCCA 1 cut(s) 1154
MspA1I CMGCKG 2 cut(s) 167, 627
MspR9I CCNGG 2 cut(s) 967, 1334
MunI CAATTG 3 cut(s) 525, 798, 1107
Mva1269I GAATGC 3 cut(s) 846, 1000, 1045
MvaI CCWGG 2 cut(s) 967, 1334
NdeII GATC 6 cut(s) 45, 672, 938, 1216, 1300, 1387
NlaIII CATG 4 cut(s) 563, 769, 883, 1396
NlaIV GGNNCC 4 cut(s) 55, 66, 370, 371
NmeAIII GCCGAG 1 cut(s) 1217
NmuCI GTSAC 3 cut(s) 141, 517, 1256
NspI RCATGY 1 cut(s) 769
PciI ACATGT 1 cut(s) 765
PcsI WCGNNNNNNNCGW 1 cut(s) 179
PctI GAATGC 3 cut(s) 846, 1000, 1045
PdmI GAANNNNTTC 1 cut(s) 325
PfeI GAWTC 2 cut(s) 892, 1422
PfoI TCCNGGA 1 cut(s) 1332
PkrI GCNGC 8 cut(s) 626, 629, 1029, 1038, 1101, 1230, 1242, 1245
PleI GAGTC 2 cut(s) 81, 290
PpsI GAGTC 2 cut(s) 81, 290
PpuMI RGGWCCY 1 cut(s) 369
PscI ACATGT 1 cut(s) 765
Psp5II RGGWCCY 1 cut(s) 369
Psp6I CCWGG 2 cut(s) 965, 1332
PspFI CCCAGC 1 cut(s) 512
PspGI CCWGG 2 cut(s) 965, 1332
PspN4I GGNNCC 4 cut(s) 55, 66, 370, 371
PspPI GGNCC 4 cut(s) 175, 369, 544, 569
PspPPI RGGWCCY 1 cut(s) 369
PsuI RGATCY 2 cut(s) 672, 1387
PvuII CAGCTG 1 cut(s) 627
RsaI GTAC 1 cut(s) 796
RsaNI GTAC 1 cut(s) 795
RseI CAYNNNNRTG 4 cut(s) 396, 564, 687, 1407
SaqAI TTAA 1 cut(s) 1368
SatI GCNGC 8 cut(s) 625, 628, 1028, 1037, 1100, 1229, 1241, 1244
Sau3AI GATC 6 cut(s) 45, 672, 938, 1216, 1300, 1387
Sau96I GGNCC 4 cut(s) 175, 369, 544, 569
SchI GAGTC 2 cut(s) 82, 290
ScrFI CCNGG 2 cut(s) 967, 1334
SfaNI GCATC 5 cut(s) 499, 571, 726, 980, 1150
SinI GGWCC 2 cut(s) 369, 569
SmiMI CAYNNNNRTG 4 cut(s) 396, 564, 687, 1407
Sse9I AATT 8 cut(s) 109, 525, 539, 587, 798, 984, 1107, 1426
SsiI CCGC 2 cut(s) 167, 1100
StyD4I CCNGG 2 cut(s) 965, 1332
StyI CCWWGG 1 cut(s) 1155
TaaI ACNGT 6 cut(s) 13, 211, 220, 259, 445, 1179
TaiI ACGT 1 cut(s) 787
TasI AATT 8 cut(s) 109, 525, 539, 587, 798, 984, 1107, 1426
TauI GCSGC 1 cut(s) 1102
TfiI GAWTC 2 cut(s) 892, 1422
Tru1I TTAA 1 cut(s) 1368
Tru9I TTAA 1 cut(s) 1368
TscAI CASTG 7 cut(s) 150, 216, 225, 262, 894, 1315, 1414
TseFI GTSAC 3 cut(s) 141, 517, 1256
TseI GCWGC 7 cut(s) 624, 627, 1027, 1036, 1228, 1240, 1243
Tsp45I GTSAC 3 cut(s) 141, 517, 1256
TspDTI ATGAA 3 cut(s) 143, 437, 834
TspGWI ACGGA 1 cut(s) 1398
TspRI CASTG 7 cut(s) 150, 216, 225, 262, 894, 1315, 1414
VpaK11BI GGWCC 2 cut(s) 369, 569
XapI RAATTY 3 cut(s) 109, 587, 984
XceI RCATGY 1 cut(s) 769
XcmI CCANNNNNNNNNTGG 2 cut(s) 710, 1340
XmnI GAANNNNTTC 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.