AT1G72810

threonine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005631
Physical Location & Seq
Reverse (-)
27397877 .. 27400474
2598 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G72810.1

Sequence Viewer

Length: 1551 bp
ATGGCCTCATTCTCTCTTCCCCACTCCGCCACCTACTTTCCTTCCCACTCTGAAACCTCACTTAAACCTCACTCCGCCGCTTCCTTCACCGTCCGTTGCACCTCCGCCTCTCCCGCCGTTCCTCCACAGACTCCACAGAAACCCCGTCGTTCACCCGATGAGAACATTCGAGACGAAGCTCGTCGCCGTCCTCATCAACTCCAAAACCTCTCCGCACGTTACGTCCCTTTCAACGCTCCACCTTCTTCAACAGAATCCTACTCCCTCGACGAGATCGTCTACCGTTCACAATCCGGTGCTCTCCTCGACGTCCAGCATGATTTCGCCGCCTTGAAACGCTACGACGGAGAATTCTGGCGTAACCTTTTCGATTCTCGCGTCGGTAAAACAAATTGGCCGTACGGATCAGGCGTGTGGTCGAAGAAAGAGTGGGTACTCCCCGAGATCGACGATGACGACATCGTCTCTGCTTTTGAAGGAAACTCAAATCTCTTCTGGGCTGAGAGATTTGGTAAACAGTATCTGCAAATGAACGATCTTTGGGTGAAACATTGTGGAATCAGCCACACTGGCTCCTTTAAAGATCTGGGCATGAGTGTTCTCGTTTCTCAGGTGAATCGTCTCCGGAAAATGAACAAACCAGTCATCGGAGTTGGTTGCGCTTCTACTGGAGATACCTCCGCTGCTCTCTCTGCTTACTGTGCTTCCGCTGGAATCCCCTCGATTGTCTTCTTACCTGCGGATAAAATCTCCATGGCTCAGCTTGTGCAGCCGATTGCAAACGGAGCGTTTGTGCTTAGTATTGACACTGATTTTGACGGATGTATGCATTTGATCAGAGAAGTTACGGCGGAGCTTCCTATTTACTTGGCGAACTCTCTCAACAGCCTCCGTCTCGAAGGTCAAAAGACGGCGGCGATTGAGATTCTGCAGCAGTTTAATTGGCAAGTTCCTGATTGGGTCATTGTTCCTGGAGGCAATCTTGGTAACATCTATGCGTTTTACAAAGGGTTTCATATGTGTAAAGAGTTAGGTCTTGTTGATCGTATCCCTAGGCTTGTTTGTGCTCAAGCTGCTAATGCAAATCCACTTTACTTGCATTACAAATCTGGGTTTAAAGAAGATTTCAATCCATTGAAAGCAAACACTACATTTGCTTCTGCTATTCAGATTGGTGACCCTGTTTCGATTGATAGAGCTGTGTATGCGTTGAAGAAATCTAATGGGATTGTTGAGGAAGCTACAGAGGAAGAGTTGATGGATGCAACAGCTCTTGCTGATTCTACTGGTATGTTTATATGTCCACATACTGGTGTTGCATTGACGGCGTTGATGAAGCTGAGAAAGTCAGGAGTTATTGGAGCGAATGATCGGACTGTGGTGGTGAGTACAGCTCATGGATTGAAGTTTACACAAAGTAAGATTGATTATCATTCTAAGAACATAAAGGAGATGGCTTGTAGATTGGCGAATCCACCAGTGAAGGTTAAGGCAAAGTTTGGTTCAGTTATGGATGTTCTCAAGGAGTATTTGAAGAGCAATGATAAATAA

Protein Analysis

516

Amino Acids

56.92

Weight (kDa)

8.23

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 156 - 464 3.5e-51 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 275, 461
AatII GACGTC 1 cut(s) 312
Acc36I ACCTGC 1 cut(s) 745
AccB7I CCANNNNNTGG 1 cut(s) 1310
AccI GTMKAC 1 cut(s) 279
AccII CGCG 1 cut(s) 378
AccIII TCCGGA 1 cut(s) 624
AclWI GGATC 1 cut(s) 412
AcoI YGGCCR 1 cut(s) 395
AcsI RAATTY 1 cut(s) 350
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 3 cut(s) 401, 435, 1390
AfiI CCNNNNNNNGG 2 cut(s) 647, 1310
AgsI TTSAA 9 cut(s) 232, 249, 334, 476, 1129, 1138, 1213, 1405, 1534
AjnI CCWGG 1 cut(s) 970
AluBI AGCT 9 cut(s) 179, 763, 856, 1073, 1199, 1241, 1271, 1339, 1394
AluI AGCT 9 cut(s) 179, 763, 856, 1073, 1199, 1241, 1271, 1339, 1394
Alw21I GWGCWC 2 cut(s) 301, 1069
Alw26I GTCTC 4 cut(s) 165, 469, 626, 899
AlwI GGATC 1 cut(s) 412
AlwNI CAGNNNCTG 1 cut(s) 523
Ama87I CYCGRG 1 cut(s) 440
Aor13HI TCCGGA 1 cut(s) 624
AoxI GGCC 2 cut(s) 3, 395
ApeKI GCWGC 4 cut(s) 683, 769, 931, 1073
ApoI RAATTY 1 cut(s) 350
ArsI GACNNNNNNTTYG 2 cut(s) 797, 829
AspA2I CCTAGG 1 cut(s) 1052
AspLEI GCGC 1 cut(s) 662
AsuHPI GGTGA 6 cut(s) 79, 144, 556, 625, 1187, 1396
AvaI CYCGRG 1 cut(s) 440
AvrII CCTAGG 1 cut(s) 1052
BbsI GAAGAC 1 cut(s) 721
Bbv12I GWGCWC 2 cut(s) 301, 1069
BbvI GCAGC 4 cut(s) 670, 781, 943, 1060
BccI CCATC 2 cut(s) 1252, 1447
BceAI ACGGC 6 cut(s) 101, 171, 382, 864, 927, 1341
BciT130I CCWGG 1 cut(s) 972
BciVI GTATCC 1 cut(s) 1058
BclI TGATCA 1 cut(s) 834
BcoDI GTCTC 4 cut(s) 165, 469, 626, 899
BfaI CTAG 1 cut(s) 1053
BfmI CTRYAG 2 cut(s) 929, 1242
BfuAI ACCTGC 1 cut(s) 745
BfuI GTATCC 1 cut(s) 1058
BglI GCCNNNNNGGC 1 cut(s) 570
BglII AGATCT 1 cut(s) 583
BisI GCNGC 7 cut(s) 78, 327, 684, 770, 915, 932, 1074
BlnI CCTAGG 1 cut(s) 1052
BlpI GCTNAGC 1 cut(s) 759
BlsI GCNGC 7 cut(s) 79, 328, 685, 771, 916, 933, 1075
Bme1390I CCNGG 1 cut(s) 972
BmeT110I CYCGRG 1 cut(s) 440
BmiI GGNNCC 1 cut(s) 574
BmrFI CCNGG 1 cut(s) 972
BmsI GCATC 1 cut(s) 1252
BpiI GAAGAC 1 cut(s) 721
BplI GAGNNNNNCTC 2 cut(s) 1378, 1410
BpmI CTGGAG 2 cut(s) 690, 993
Bpu1102I GCTNAGC 1 cut(s) 759
BpuEI CTTGAG 2 cut(s) 1053, 1505
BsaBI GATNNNNATC 1 cut(s) 1128
BsaHI GRCGYC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 753, 1052
BsaWI WCCGGW 2 cut(s) 293, 624
BsaXI ACNNNNNCTCC 4 cut(s) 557, 587, 777, 807
Bsc4I CCNNNNNNNGG 2 cut(s) 647, 1310
Bse1I ACTGG 6 cut(s) 574, 641, 673, 1291, 1315, 1478
Bse3DI GCAATG 1 cut(s) 1546
Bse8I GATNNNNATC 1 cut(s) 1128
BseAI TCCGGA 1 cut(s) 624
BseBI CCWGG 1 cut(s) 972
BseDI CCNNGG 2 cut(s) 753, 1052
BseGI GGATG 3 cut(s) 827, 1267, 1519
BseJI GATNNNNATC 1 cut(s) 1128
BseLI CCNNNNNNNGG 2 cut(s) 647, 1310
BseMI GCAATG 1 cut(s) 1546
BseMII CTCAG 4 cut(s) 492, 623, 773, 1331
BseNI ACTGG 6 cut(s) 574, 641, 673, 1291, 1315, 1478
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 4 cut(s) 670, 781, 943, 1060
BsgI GTGCAG 1 cut(s) 788
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 2 cut(s) 5, 397
BsiHKAI GWGCWC 2 cut(s) 301, 1069
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 2 cut(s) 294, 625
BsiWI CGTACG 1 cut(s) 399
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 2 cut(s) 647, 1310
BsmAI GTCTC 4 cut(s) 165, 469, 626, 899
BsmBI CGTCTC 4 cut(s) 165, 469, 626, 899
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 2 cut(s) 5, 397
BsoBI CYCGRG 1 cut(s) 440
Bsp1286I GDGCHC 2 cut(s) 301, 1069
Bsp13I TCCGGA 1 cut(s) 624
Bsp143I GATC 8 cut(s) 273, 404, 444, 535, 583, 834, 1042, 1369
Bsp1720I GCTNAGC 1 cut(s) 759
Bsp19I CCATGG 1 cut(s) 753
BspANI GGCC 2 cut(s) 5, 397
BspCNI CTCAG 4 cut(s) 493, 622, 772, 1332
BspEI TCCGGA 1 cut(s) 624
BspFNI CGCG 1 cut(s) 378
BspLI GGNNCC 1 cut(s) 574
BspMAI CTGCAG 1 cut(s) 933
BspMI ACCTGC 1 cut(s) 745
BspPI GGATC 1 cut(s) 412
BspQI GCTCTTC 1 cut(s) 1529
BsrDI GCAATG 1 cut(s) 1546
BsrI ACTGG 6 cut(s) 574, 641, 673, 1291, 1315, 1478
BssECI CCNNGG 2 cut(s) 753, 1052
BssMI GATC 8 cut(s) 273, 404, 444, 535, 583, 834, 1042, 1369
BssNI GRCGYC 1 cut(s) 309
BssT1I CCWWGG 2 cut(s) 753, 1052
Bst2UI CCWGG 1 cut(s) 972
Bst4CI ACNGT 5 cut(s) 91, 284, 519, 701, 1378
Bst6I CTCTTC 4 cut(s) 21, 497, 1245, 1529
BstACI GRCGYC 1 cut(s) 309
BstDEI CTNAG 6 cut(s) 501, 609, 759, 797, 1340, 1437
BstDSI CCRYGG 1 cut(s) 753
BstEII GGTNACC 1 cut(s) 1175
BstF5I GGATG 3 cut(s) 827, 1267, 1519
BstFNI CGCG 1 cut(s) 378
BstHHI GCGC 1 cut(s) 662
BstKTI GATC 8 cut(s) 276, 407, 447, 538, 586, 837, 1045, 1372
BstMAI GTCTC 4 cut(s) 165, 469, 626, 899
BstMBI GATC 8 cut(s) 273, 404, 444, 535, 583, 834, 1042, 1369
BstNI CCWGG 1 cut(s) 972
BstPI GGTNACC 1 cut(s) 1175
BstSCI CCNGG 1 cut(s) 970
BstSFI CTRYAG 2 cut(s) 929, 1242
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 4 cut(s) 670, 781, 943, 1060
BstV2I GAAGAC 1 cut(s) 721
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BsuI GTATCC 1 cut(s) 1058
BsuRI GGCC 2 cut(s) 5, 397
BtgI CCRYGG 1 cut(s) 753
BtsCI GGATG 3 cut(s) 827, 1267, 1519
BtsIMutI CAGTG 3 cut(s) 567, 807, 1485
BveI ACCTGC 1 cut(s) 745
CaiI CAGNNNCTG 1 cut(s) 523
CfoI GCGC 1 cut(s) 662
CseI GACGC 1 cut(s) 367
Csp6I GTAC 3 cut(s) 400, 434, 1389
CspCI CAANNNNNGTGG 2 cut(s) 1077, 1112
CviAII CATG 4 cut(s) 317, 592, 754, 1397
CviQI GTAC 3 cut(s) 400, 434, 1389
DdeI CTNAG 6 cut(s) 501, 609, 759, 797, 1340, 1437
DpnI GATC 8 cut(s) 275, 406, 446, 537, 585, 836, 1044, 1371
DpnII GATC 8 cut(s) 273, 404, 444, 535, 583, 834, 1042, 1369
DraI TTTAAA 2 cut(s) 580, 1117
DrdI GACNNNNNNGTC 2 cut(s) 275, 461
DseDI GACNNNNNNGTC 2 cut(s) 275, 461
EaeI YGGCCR 1 cut(s) 395
Eam1104I CTCTTC 4 cut(s) 21, 497, 1245, 1529
EarI CTCTTC 4 cut(s) 21, 497, 1245, 1529
EciI GGCGGA 4 cut(s) 16, 64, 94, 866
Eco130I CCWWGG 2 cut(s) 753, 1052
Eco88I CYCGRG 1 cut(s) 440
Eco91I GGTNACC 1 cut(s) 1175
EcoO65I GGTNACC 1 cut(s) 1175
EcoRI GAATTC 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 970
EcoT14I CCWWGG 2 cut(s) 753, 1052
EcoT22I ATGCAT 1 cut(s) 831
ErhI CCWWGG 2 cut(s) 753, 1052
Esp3I CGTCTC 4 cut(s) 165, 469, 626, 899
FaeI CATG 4 cut(s) 320, 595, 757, 1400
FaqI GGGAC 1 cut(s) 209
FatI CATG 4 cut(s) 316, 591, 753, 1396
FauI CCCGC 1 cut(s) 121
FauNDI CATATG 1 cut(s) 1017
FbaI TGATCA 1 cut(s) 834
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 7 cut(s) 78, 327, 684, 770, 915, 932, 1074
FokI GGATG 3 cut(s) 834, 1274, 1526
Fsp4HI GCNGC 7 cut(s) 78, 327, 684, 770, 915, 932, 1074
FspBI CTAG 1 cut(s) 1053
GlaI GCGC 1 cut(s) 661
GluI GCNGC 7 cut(s) 78, 327, 684, 770, 915, 932, 1074
GsuI CTGGAG 2 cut(s) 690, 993
HaeIII GGCC 2 cut(s) 5, 397
HapII CCGG 2 cut(s) 294, 625
HgaI GACGC 1 cut(s) 367
HhaI GCGC 1 cut(s) 662
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 4 cut(s) 320, 595, 757, 1400
Hin6I GCGC 1 cut(s) 660
HinP1I GCGC 1 cut(s) 660
HinfI GANTC 9 cut(s) 130, 254, 371, 558, 616, 714, 925, 1280, 1471
HpaII CCGG 2 cut(s) 294, 625
HphI GGTGA 6 cut(s) 79, 144, 556, 625, 1187, 1396
Hpy166II GTNNAC 6 cut(s) 152, 280, 287, 515, 1304, 1410
Hpy188I TCNGA 5 cut(s) 52, 650, 839, 1170, 1374
Hpy188III TCNNGA 5 cut(s) 170, 625, 896, 953, 1350
Hpy8I GTNNAC 6 cut(s) 152, 280, 287, 515, 1304, 1410
Hpy99I CGWCG 7 cut(s) 150, 186, 272, 311, 347, 383, 452
HpyAV CCTTC 6 cut(s) 51, 94, 252, 470, 893, 1477
HpyCH4III ACNGT 5 cut(s) 91, 284, 519, 701, 1378
HpyCH4IV ACGT 3 cut(s) 217, 222, 309
HpyF3I CTNAG 6 cut(s) 501, 609, 759, 797, 1340, 1437
HpySE526I ACGT 3 cut(s) 217, 222, 309
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 4 cut(s) 320, 595, 757, 1400
HspAI GCGC 1 cut(s) 660
Kpn2I TCCGGA 1 cut(s) 624
Ksp22I TGATCA 1 cut(s) 834
Kzo9I GATC 8 cut(s) 273, 404, 444, 535, 583, 834, 1042, 1369
LguI GCTCTTC 1 cut(s) 1529
LmnI GCTCC 5 cut(s) 241, 578, 785, 853, 1361
Lsp1109I GCAGC 4 cut(s) 670, 781, 943, 1060
LweI GCATC 1 cut(s) 1252
MaeI CTAG 1 cut(s) 1053
MaeII ACGT 3 cut(s) 217, 222, 309
MaeIII GTNAC 5 cut(s) 218, 359, 844, 986, 1175
MalI GATC 8 cut(s) 275, 406, 446, 537, 585, 836, 1044, 1371
MboI GATC 8 cut(s) 273, 404, 444, 535, 583, 834, 1042, 1369
MboII GAAGA 9 cut(s) 8, 237, 433, 484, 721, 1133, 1225, 1262, 1546
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 2 cut(s) 301, 1069
MluCI AATT 3 cut(s) 350, 391, 940
MlyI GAGTC 1 cut(s) 124
Mph1103I ATGCAT 1 cut(s) 831
MroI TCCGGA 1 cut(s) 624
MseI TTAA 5 cut(s) 63, 579, 939, 1116, 1488
MslI CAYNNNNRTG 1 cut(s) 1311
MspA1I CMGCKG 2 cut(s) 683, 710
MspI CCGG 2 cut(s) 294, 625
MspR9I CCNGG 1 cut(s) 972
MvaI CCWGG 1 cut(s) 972
MvnI CGCG 1 cut(s) 378
NcoI CCATGG 1 cut(s) 753
NdeI CATATG 1 cut(s) 1017
NdeII GATC 8 cut(s) 273, 404, 444, 535, 583, 834, 1042, 1369
NlaIII CATG 4 cut(s) 320, 595, 757, 1400
NlaIV GGNNCC 1 cut(s) 574
NmuCI GTSAC 1 cut(s) 1175
NsiI ATGCAT 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 1529
PcsI WCGNNNNNNNCGW 4 cut(s) 273, 375, 408, 453
PfeI GAWTC 8 cut(s) 254, 371, 558, 616, 714, 925, 1280, 1471
Pfl23II CGTACG 1 cut(s) 399
PflFI GACNNNGTC 1 cut(s) 461
PflMI CCANNNNNTGG 1 cut(s) 1310
PfoI TCCNGGA 1 cut(s) 970
PkrI GCNGC 7 cut(s) 79, 328, 685, 771, 916, 933, 1075
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp6I CCWGG 1 cut(s) 970
PspEI GGTNACC 1 cut(s) 1175
PspGI CCWGG 1 cut(s) 970
PspLI CGTACG 1 cut(s) 399
PspN4I GGNNCC 1 cut(s) 574
PstI CTGCAG 1 cut(s) 933
PstNI CAGNNNCTG 1 cut(s) 523
PsuI RGATCY 1 cut(s) 583
PsyI GACNNNGTC 1 cut(s) 461
RsaI GTAC 3 cut(s) 401, 435, 1390
RsaNI GTAC 3 cut(s) 400, 434, 1389
RseI CAYNNNNRTG 1 cut(s) 1311
SapI GCTCTTC 1 cut(s) 1529
SaqAI TTAA 5 cut(s) 63, 579, 939, 1116, 1488
SatI GCNGC 7 cut(s) 78, 327, 684, 770, 915, 932, 1074
Sau3AI GATC 8 cut(s) 273, 404, 444, 535, 583, 834, 1042, 1369
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 972
SduI GDGCHC 2 cut(s) 301, 1069
SfaNI GCATC 1 cut(s) 1252
SfcI CTRYAG 2 cut(s) 929, 1242
SmiMI CAYNNNNRTG 1 cut(s) 1311
SmlI CTYRAG 2 cut(s) 1068, 1520
SmoI CTYRAG 2 cut(s) 1068, 1520
Sse9I AATT 3 cut(s) 350, 391, 940
SspMI CTAG 1 cut(s) 1053
StyD4I CCNGG 1 cut(s) 970
StyI CCWWGG 2 cut(s) 753, 1052
TaaI ACNGT 5 cut(s) 91, 284, 519, 701, 1378
TaiI ACGT 3 cut(s) 220, 225, 312
TaqI TCGA 9 cut(s) 169, 267, 306, 369, 419, 447, 722, 897, 1187
TasI AATT 3 cut(s) 350, 391, 940
TatI WGTACW 1 cut(s) 1388
TauI GCSGC 3 cut(s) 80, 329, 917
TfiI GAWTC 8 cut(s) 254, 371, 558, 616, 714, 925, 1280, 1471
Tru1I TTAA 5 cut(s) 63, 579, 939, 1116, 1488
Tru9I TTAA 5 cut(s) 63, 579, 939, 1116, 1488
TscAI CASTG 3 cut(s) 574, 814, 1485
TseFI GTSAC 1 cut(s) 1175
TseI GCWGC 4 cut(s) 683, 769, 931, 1073
Tsp45I GTSAC 1 cut(s) 1175
TspDTI ATGAA 4 cut(s) 545, 647, 1004, 1349
TspGWI ACGGA 6 cut(s) 83, 360, 417, 798, 834, 881
TspRI CASTG 3 cut(s) 574, 814, 1485
Tth111I GACNNNGTC 1 cut(s) 461
Van91I CCANNNNNTGG 1 cut(s) 1310
XapI RAATTY 1 cut(s) 350
XmaJI CCTAGG 1 cut(s) 1052
XmiI GTMKAC 1 cut(s) 279
XspI CTAG 1 cut(s) 1053
ZraI GACGTC 1 cut(s) 310
Zsp2I ATGCAT 1 cut(s) 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.