FvH4_6g34290

Threonine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005631
Physical Location & Seq
Reverse (-)
27048593 .. 27050609
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g34290.t1

Sequence Viewer

Length: 1572 bp
ATGGCGTCTTCCTCCCTCTTCCAATCCCCCTCCTTCTCTCTCAAACCCCAAACCCCCATCTCCCCTCCCTCCCCCAAACCCCATTTCACCACCGTCAAATGCTCCTCCTCCTCCAAATGCCGCGACAACATCCGCGACGACGCCCACCGCCACAACACCACCCACCCCCACCACTTCTCCGCCAAATACGTCCCCTTCAACTCCTCCTCCTCAAACGACACGTCGTCCTCCGAATCCTACTCCCTCGACGAGATCGTCTACCGCTCCCAGTCCGGCGGCCTCCTCGACGTCCAGCACGACATGTCCGCCCTCAAAAACTACGACGGCCGCTACTGGCGCGACCTCTTTGACTCCCGCCTCGGCAAAACCACCTGGCCCTACGGCTCCGGCGTCTGGTCCAAAAAGGAGTGGGTCCTCCCCGAGATCGACTCCGACGACATCGTCTCCGCCTTCGAAGGCAACTCCAACCTCTTCTGGGCCGAGCGCTACGGCAAGCAAGTCCTCTCCATGAACGAGCTCTGGGTCAAGCACTGCGGCATCAGCCACACCGGCTCCTTCAAGGACCTCGGCATGACCGTCCTCGTCAGCCAGGTCAACCGCCTCCGCAAAATGAACCGCCCCGTCGTCGGCGTCGGTTGCGCCTCCACCGGCGACACCTCCGCCGCGTTGTCCGCCTACTGCGCCGCAGCCGGGATCCCCTCCATTGTGTTCCTCCCGGCGAACAAGATCTCCGTGGCGCAGCTGGTCCAGCCGATTGCGAATGGCGCCTTTGTGCTGAGCATTGATACCGATTTCGACGGCTGTATGCAGCTGATTAGGGAAGTCACGGCGGAGCTGCCGATTTATCTGGCGAACTCTCTCAACAGTTTGAGGCTTGAGGGACAGAAAACAGCTGCCATTGAGATCCTGCAGCAGTTCGACTGGCAGGTCCCGGATTGGGTCATAGTTCCGGGAGGAAATCTCGGAAACATTTATGCATTTTACAAAGGGTTTCACATGTGCAAGGAGCTGGGGCTGGTTGATAGGATTCCGAGGCTAGTCTGTGCTCAAGCTGCGAATGCGAATCCTTTGTATCTGCATTACAAGTCAGGGTGGGATGAGGAGTTCAGGCCGGTGAAGGCCAATACTACATTTGCGTCTGCCATTCAGATTGGGGACCCTGTTTCGATTGACAGGGCTGTGTATGCGCTGAAGAAGTCGGATGGGATTGTGGAGGAGGCGACTGAGGAGGAGCTGATGGATGCAATGGCTCAGGCGGATTCGACTGGCATGTTTATATGTCCTCACACTGGCGTGGCGCTGGCGGCGTTGATCAAGCTGAGGAATAAGGGGGTTATAGGGGCGACAGAGAGGACTGTGGTGGTGAGCACGGCTCACGGGTTGAAGTTCACACAGGCGAAGATTGATTATCACTCTAAGGAGATTCCGGATTTGGCTTGCCGGTTTGCTAACCCGCCGGTGCAAGTGAAGGCTGATTTTGGGTCGGTTATGGATGTTTTGAAGAAGTACTTGAGGCAAGCAGAAAGTTTTTGGATTCGGACTCGGATTAGACGAATATCCTACGAGCATTGA

Protein Analysis

524

Amino Acids

57.91

Weight (kDa)

7.22

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 149 - 457 4.4e-52 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 254, 440, 926
AatII GACGTC 1 cut(s) 291
Acc36I ACCTGC 1 cut(s) 916
AccB1I GGYRCC 1 cut(s) 764
AccBSI CCGCTC 1 cut(s) 264
AccI GTMKAC 1 cut(s) 258
AccII CGCG 4 cut(s) 123, 135, 339, 665
AccIII TCCGGA 1 cut(s) 1426
AclWI GGATC 3 cut(s) 688, 701, 898
AcoI YGGCCR 1 cut(s) 325
AcuI CTGAAG 1 cut(s) 1211
AcyI GRCGYC 6 cut(s) 5, 141, 288, 390, 630, 765
AfaI GTAC 1 cut(s) 1508
AfeI AGCGCT 1 cut(s) 485
AfiI CCNNNNNNNGG 6 cut(s) 393, 475, 626, 690, 937, 1289
AflIII ACRYGT 3 cut(s) 219, 300, 996
AgsI TTSAA 4 cut(s) 199, 559, 1384, 1501
AhdI GACNNNNNGTC 1 cut(s) 223
AjiI CACGTC 1 cut(s) 222
AjnI CCWGG 2 cut(s) 371, 588
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AleI CACNNNNGTG 1 cut(s) 1292
AloI GAACNNNNNNTCC 4 cut(s) 713, 745, 1088, 1120
AluBI AGCT 9 cut(s) 517, 742, 811, 835, 893, 1009, 1052, 1234, 1318
AluI AGCT 9 cut(s) 517, 742, 811, 835, 893, 1009, 1052, 1234, 1318
Alw21I GWGCWC 3 cut(s) 519, 1048, 1370
Alw26I GTCTC 1 cut(s) 448
AlwI GGATC 3 cut(s) 688, 701, 898
Ama87I CYCGRG 1 cut(s) 419
Aor13HI TCCGGA 1 cut(s) 1426
Aor51HI AGCGCT 1 cut(s) 485
AoxI GGCC 6 cut(s) 277, 325, 374, 477, 1109, 1119
ApeKI GCWGC 7 cut(s) 686, 739, 808, 835, 893, 910, 1052
ArsI GACNNNNNNTTYG 2 cut(s) 206, 238
AspLEI GCGC 8 cut(s) 339, 486, 641, 683, 739, 767, 1189, 1300
AspS9I GGNCC 8 cut(s) 375, 396, 412, 477, 562, 745, 928, 1156
AsuC2I CCSGG 4 cut(s) 691, 716, 932, 951
AsuHPI GGTGA 3 cut(s) 79, 1126, 1375
AsuII TTCGAA 1 cut(s) 453
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 6 cut(s) 396, 412, 562, 745, 928, 1156
BamHI GGATCC 1 cut(s) 693
BanI GGYRCC 1 cut(s) 764
BanII GRGCYC 1 cut(s) 519
Bbv12I GWGCWC 3 cut(s) 519, 1048, 1370
BbvCI CCTCAGC 1 cut(s) 1319
BbvI GCAGC 7 cut(s) 698, 751, 820, 822, 880, 922, 1039
BccI CCATC 3 cut(s) 65, 1196, 1231
BceAI ACGGC 6 cut(s) 340, 397, 505, 814, 843, 1386
BciT130I CCWGG 2 cut(s) 373, 590
BclI TGATCA 1 cut(s) 1311
BcnI CCSGG 4 cut(s) 691, 716, 932, 951
BcoDI GTCTC 1 cut(s) 448
BfaI CTAG 1 cut(s) 1037
BfmI CTRYAG 1 cut(s) 908
BfoI RGCGCY 3 cut(s) 487, 768, 1301
BfuAI ACCTGC 1 cut(s) 916
BglI GCCNNNNNGGC 1 cut(s) 549
BglII AGATCT 1 cut(s) 726
BlpI GCTNAGC 1 cut(s) 776
BmcAI AGTACT 1 cut(s) 1508
Bme1390I CCNGG 6 cut(s) 373, 590, 691, 716, 932, 951
Bme18I GGWCC 6 cut(s) 396, 412, 562, 745, 928, 1156
BmeRI GACNNNNNGTC 1 cut(s) 223
BmeT110I CYCGRG 1 cut(s) 419
BmgBI CACGTC 1 cut(s) 222
BmgT120I GGNCC 8 cut(s) 375, 396, 412, 477, 562, 745, 928, 1156
BmiI GGNNCC 8 cut(s) 385, 413, 553, 695, 766, 930, 1157, 1158
BmrFI CCNGG 6 cut(s) 373, 590, 691, 716, 932, 951
BmrI ACTGGG 1 cut(s) 262
BmsI GCATC 2 cut(s) 546, 1231
BmuI ACTGGG 1 cut(s) 262
BplI GAGNNNNNCTC 6 cut(s) 413, 445, 945, 977, 1357, 1389
Bpu10I CCTNAGC 2 cut(s) 1251, 1319
Bpu1102I GCTNAGC 1 cut(s) 776
Bpu14I TTCGAA 1 cut(s) 453
BpuEI CTTGAG 3 cut(s) 896, 1032, 1531
BpuMI CCSGG 4 cut(s) 691, 716, 932, 951
BsaHI GRCGYC 6 cut(s) 5, 141, 288, 390, 630, 765
BsaJI CCNNGG 4 cut(s) 358, 565, 732, 1031
BsaWI WCCGGW 1 cut(s) 1426
Bsc4I CCNNNNNNNGG 6 cut(s) 393, 475, 626, 690, 937, 1289
Bse118I RCCGGY 5 cut(s) 548, 647, 1111, 1440, 1456
Bse1I ACTGG 5 cut(s) 268, 338, 926, 1270, 1294
Bse3DI GCAATG 1 cut(s) 1251
BseAI TCCGGA 1 cut(s) 1426
BseBI CCWGG 2 cut(s) 373, 590
BseDI CCNNGG 4 cut(s) 358, 565, 732, 1031
BseGI GGATG 5 cut(s) 129, 1102, 1207, 1246, 1498
BseLI CCNNNNNNNGG 6 cut(s) 393, 475, 626, 690, 937, 1289
BseMI GCAATG 1 cut(s) 1251
BseMII CTCAG 4 cut(s) 767, 1215, 1265, 1310
BseNI ACTGG 5 cut(s) 268, 338, 926, 1270, 1294
BseX3I CGGCCG 1 cut(s) 325
BseXI GCAGC 7 cut(s) 698, 751, 820, 822, 880, 922, 1039
BseYI CCCAGC 1 cut(s) 1009
Bsh1236I CGCG 4 cut(s) 123, 135, 339, 665
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 6 cut(s) 279, 327, 376, 479, 1111, 1121
BshNI GGYRCC 1 cut(s) 764
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 3 cut(s) 519, 1048, 1370
BsiHKCI CYCGRG 1 cut(s) 419
BslFI GGGAC 4 cut(s) 176, 894, 914, 1169
BslI CCNNNNNNNGG 6 cut(s) 393, 475, 626, 690, 937, 1289
BsmAI GTCTC 1 cut(s) 448
BsmBI CGTCTC 1 cut(s) 448
BsmFI GGGAC 4 cut(s) 176, 894, 914, 1169
BsmI GAATGC 1 cut(s) 1063
BsnI GGCC 6 cut(s) 279, 327, 376, 479, 1111, 1121
BsoBI CYCGRG 1 cut(s) 419
Bsp119I TTCGAA 1 cut(s) 453
Bsp1286I GDGCHC 3 cut(s) 519, 1048, 1370
Bsp13I TCCGGA 1 cut(s) 1426
Bsp143I GATC 6 cut(s) 252, 423, 693, 726, 903, 1311
Bsp1720I GCTNAGC 1 cut(s) 776
BspANI GGCC 6 cut(s) 279, 327, 376, 479, 1111, 1121
BspCNI CTCAG 4 cut(s) 768, 1216, 1264, 1311
BspEI TCCGGA 1 cut(s) 1426
BspFNI CGCG 4 cut(s) 123, 135, 339, 665
BspLI GGNNCC 8 cut(s) 385, 413, 553, 695, 766, 930, 1157, 1158
BspMAI CTGCAG 1 cut(s) 912
BspMI ACCTGC 1 cut(s) 916
BspPI GGATC 3 cut(s) 688, 701, 898
BspT104I TTCGAA 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 764
BsrBI CCGCTC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 1251
BsrFI RCCGGY 5 cut(s) 548, 647, 1111, 1440, 1456
BsrI ACTGG 5 cut(s) 268, 338, 926, 1270, 1294
BssAI RCCGGY 5 cut(s) 548, 647, 1111, 1440, 1456
BssECI CCNNGG 4 cut(s) 358, 565, 732, 1031
BssMI GATC 6 cut(s) 252, 423, 693, 726, 903, 1311
BssNI GRCGYC 6 cut(s) 5, 141, 288, 390, 630, 765
Bst2UI CCWGG 2 cut(s) 373, 590
Bst4CI ACNGT 4 cut(s) 94, 577, 866, 1357
Bst6I CTCTTC 2 cut(s) 23, 476
BstACI GRCGYC 6 cut(s) 5, 141, 288, 390, 630, 765
BstBI TTCGAA 1 cut(s) 453
BstC8I GCNNGC 4 cut(s) 494, 1302, 1438, 1518
BstDEI CTNAG 5 cut(s) 776, 1224, 1251, 1319, 1416
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 5 cut(s) 129, 1102, 1207, 1246, 1498
BstFNI CGCG 4 cut(s) 123, 135, 339, 665
BstH2I RGCGCY 3 cut(s) 487, 768, 1301
BstHHI GCGC 8 cut(s) 339, 486, 641, 683, 739, 767, 1189, 1300
BstKTI GATC 6 cut(s) 255, 426, 696, 729, 906, 1314
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 6 cut(s) 252, 423, 693, 726, 903, 1311
BstMCI CGRYCG 1 cut(s) 328
BstNI CCWGG 2 cut(s) 373, 590
BstNSI RCATGY 3 cut(s) 304, 1000, 1273
BstSCI CCNGG 6 cut(s) 371, 588, 689, 714, 930, 949
BstSFI CTRYAG 1 cut(s) 908
BstUI CGCG 4 cut(s) 123, 135, 339, 665
BstV1I GCAGC 7 cut(s) 698, 751, 820, 822, 880, 922, 1039
BstX2I RGATCY 3 cut(s) 693, 726, 903
BstYI RGATCY 3 cut(s) 693, 726, 903
BstZI CGGCCG 1 cut(s) 325
BsuRI GGCC 6 cut(s) 279, 327, 376, 479, 1111, 1121
BtgI CCRYGG 1 cut(s) 732
BtrI CACGTC 1 cut(s) 222
BtsCI GGATG 5 cut(s) 129, 1102, 1207, 1246, 1498
BtsI GCAGTG 1 cut(s) 529
BtsIMutI CAGTG 2 cut(s) 529, 1287
BveI ACCTGC 1 cut(s) 916
Cac8I GCNNGC 4 cut(s) 494, 1302, 1438, 1518
CfoI GCGC 8 cut(s) 339, 486, 641, 683, 739, 767, 1189, 1300
Cfr10I RCCGGY 5 cut(s) 548, 647, 1111, 1440, 1456
Cfr13I GGNCC 8 cut(s) 375, 396, 412, 477, 562, 745, 928, 1156
CseI GACGC 4 cut(s) 149, 379, 619, 1125
Csp6I GTAC 1 cut(s) 1507
CviAII CATG 5 cut(s) 301, 508, 571, 997, 1270
CviQI GTAC 1 cut(s) 1507
DdeI CTNAG 5 cut(s) 776, 1224, 1251, 1319, 1416
DinI GGCGCC 1 cut(s) 766
DpnI GATC 6 cut(s) 254, 425, 695, 728, 905, 1313
DpnII GATC 6 cut(s) 252, 423, 693, 726, 903, 1311
DrdI GACNNNNNNGTC 3 cut(s) 254, 440, 926
DriI GACNNNNNGTC 1 cut(s) 223
DseDI GACNNNNNNGTC 3 cut(s) 254, 440, 926
EaeI YGGCCR 1 cut(s) 325
EagI CGGCCG 1 cut(s) 325
Eam1104I CTCTTC 2 cut(s) 23, 476
Eam1105I GACNNNNNGTC 1 cut(s) 223
EarI CTCTTC 2 cut(s) 23, 476
EciI GGCGGA 7 cut(s) 169, 295, 436, 649, 661, 845, 1271
Ecl136II GAGCTC 1 cut(s) 517
EclXI CGGCCG 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 519
Eco47I GGWCC 6 cut(s) 396, 412, 562, 745, 928, 1156
Eco47III AGCGCT 1 cut(s) 485
Eco52I CGGCCG 1 cut(s) 325
Eco53kI GAGCTC 1 cut(s) 517
Eco57I CTGAAG 1 cut(s) 1211
Eco88I CYCGRG 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 517
EcoO109I RGGNCCY 4 cut(s) 412, 562, 928, 1156
EcoRII CCWGG 2 cut(s) 371, 588
EcoT22I ATGCAT 1 cut(s) 979
EcoT38I GRGCYC 1 cut(s) 519
EgeI GGCGCC 1 cut(s) 766
EheI GGCGCC 1 cut(s) 766
Esp3I CGTCTC 1 cut(s) 448
FaeI CATG 5 cut(s) 304, 511, 574, 1000, 1273
FalI AAGNNNNNCTT 2 cut(s) 1493, 1525
FaqI GGGAC 4 cut(s) 176, 894, 914, 1169
FatI CATG 5 cut(s) 300, 507, 570, 996, 1269
FauI CCCGC 2 cut(s) 362, 1461
FbaI TGATCA 1 cut(s) 1311
FblI GTMKAC 1 cut(s) 258
FokI GGATG 5 cut(s) 116, 1109, 1214, 1253, 1505
FriOI GRGCYC 1 cut(s) 519
FspBI CTAG 1 cut(s) 1037
GlaI GCGC 8 cut(s) 338, 485, 640, 682, 738, 766, 1188, 1299
GsaI CCCAGC 1 cut(s) 1013
HaeII RGCGCY 3 cut(s) 487, 768, 1301
HaeIII GGCC 6 cut(s) 279, 327, 376, 479, 1111, 1121
HgaI GACGC 4 cut(s) 149, 379, 619, 1125
HhaI GCGC 8 cut(s) 339, 486, 641, 683, 739, 767, 1189, 1300
Hin1I GRCGYC 6 cut(s) 5, 141, 288, 390, 630, 765
Hin1II CATG 5 cut(s) 304, 511, 574, 1000, 1273
Hin6I GCGC 8 cut(s) 337, 484, 639, 681, 737, 765, 1187, 1298
HinP1I GCGC 8 cut(s) 337, 484, 639, 681, 737, 765, 1187, 1298
HincII GTYRAC 1 cut(s) 595
HindII GTYRAC 1 cut(s) 595
HinfI GANTC 9 cut(s) 233, 350, 428, 1027, 1063, 1259, 1423, 1534, 1540
HphI GGTGA 3 cut(s) 79, 1126, 1375
Hpy166II GTNNAC 3 cut(s) 259, 595, 1389
Hpy188I TCNGA 8 cut(s) 232, 433, 965, 1032, 1149, 1201, 1539, 1545
Hpy188III TCNNGA 1 cut(s) 1427
Hpy8I GTNNAC 3 cut(s) 259, 595, 1389
HpyAV CCTTC 7 cut(s) 43, 205, 449, 460, 565, 1111, 1462
HpyCH4III ACNGT 4 cut(s) 94, 577, 866, 1357
HpyCH4IV ACGT 3 cut(s) 189, 221, 288
HpyCH4V TGCA 7 cut(s) 808, 910, 977, 1002, 1078, 1244, 1462
HpyF3I CTNAG 5 cut(s) 776, 1224, 1251, 1319, 1416
HpySE526I ACGT 3 cut(s) 189, 221, 288
Hsp92I GRCGYC 6 cut(s) 5, 141, 288, 390, 630, 765
Hsp92II CATG 5 cut(s) 304, 511, 574, 1000, 1273
HspAI GCGC 8 cut(s) 337, 484, 639, 681, 737, 765, 1187, 1298
KasI GGCGCC 1 cut(s) 764
KflI GGGWCCC 1 cut(s) 1156
Kpn2I TCCGGA 1 cut(s) 1426
Ksp22I TGATCA 1 cut(s) 1311
Kzo9I GATC 6 cut(s) 252, 423, 693, 726, 903, 1311
LmnI GCTCC 7 cut(s) 107, 269, 389, 557, 832, 1006, 1231
Lsp1109I GCAGC 7 cut(s) 698, 751, 820, 822, 880, 922, 1039
LweI GCATC 2 cut(s) 546, 1231
MaeI CTAG 1 cut(s) 1037
MaeII ACGT 3 cut(s) 189, 221, 288
MaeIII GTNAC 1 cut(s) 823
MalI GATC 6 cut(s) 254, 425, 695, 728, 905, 1313
MbiI CCGCTC 1 cut(s) 264
MboI GATC 6 cut(s) 252, 423, 693, 726, 903, 1311
MboII GAAGA 5 cut(s) 10, 463, 1204, 1411, 1513
MflI RGATCY 3 cut(s) 693, 726, 903
MhlI GDGCHC 3 cut(s) 519, 1048, 1370
Mly113I GGCGCC 1 cut(s) 765
MlyI GAGTC 3 cut(s) 344, 422, 1534
MmeI TCCRAC 3 cut(s) 456, 489, 1179
Mph1103I ATGCAT 1 cut(s) 979
MroI TCCGGA 1 cut(s) 1426
MslI CAYNNNNRTG 1 cut(s) 1292
MspA1I CMGCKG 3 cut(s) 742, 811, 893
MspR9I CCNGG 6 cut(s) 373, 590, 691, 716, 932, 951
Mva1269I GAATGC 1 cut(s) 1063
MvaI CCWGG 2 cut(s) 373, 590
MvnI CGCG 4 cut(s) 123, 135, 339, 665
NarI GGCGCC 1 cut(s) 765
NciI CCSGG 4 cut(s) 691, 716, 932, 951
NdeII GATC 6 cut(s) 252, 423, 693, 726, 903, 1311
NlaIII CATG 5 cut(s) 304, 511, 574, 1000, 1273
NlaIV GGNNCC 8 cut(s) 385, 413, 553, 695, 766, 930, 1157, 1158
NmeAIII GCCGAG 3 cut(s) 339, 505, 546
NmuCI GTSAC 1 cut(s) 823
NsiI ATGCAT 1 cut(s) 979
NspI RCATGY 3 cut(s) 304, 1000, 1273
NspV TTCGAA 1 cut(s) 453
OliI CACNNNNGTG 1 cut(s) 1292
PciI ACATGT 2 cut(s) 300, 996
PcsI WCGNNNNNNNCGW 6 cut(s) 252, 294, 387, 432, 573, 1549
PctI GAATGC 1 cut(s) 1063
PfeI GAWTC 6 cut(s) 233, 1027, 1063, 1259, 1423, 1534
PflFI GACNNNGTC 1 cut(s) 440
PfoI TCCNGGA 2 cut(s) 930, 949
PleI GAGTC 3 cut(s) 344, 422, 1534
PluTI GGCGCC 1 cut(s) 768
PpsI GAGTC 3 cut(s) 344, 422, 1534
PpuMI RGGWCCY 4 cut(s) 412, 562, 928, 1156
PscI ACATGT 2 cut(s) 300, 996
Psp124BI GAGCTC 1 cut(s) 519
Psp5II RGGWCCY 4 cut(s) 412, 562, 928, 1156
Psp6I CCWGG 2 cut(s) 371, 588
PspFI CCCAGC 1 cut(s) 1009
PspGI CCWGG 2 cut(s) 371, 588
PspN4I GGNNCC 8 cut(s) 385, 413, 553, 695, 766, 930, 1157, 1158
PspPI GGNCC 8 cut(s) 375, 396, 412, 477, 562, 745, 928, 1156
PspPPI RGGWCCY 4 cut(s) 412, 562, 928, 1156
PstI CTGCAG 1 cut(s) 912
PsuI RGATCY 3 cut(s) 693, 726, 903
PsyI GACNNNGTC 1 cut(s) 440
PvuII CAGCTG 3 cut(s) 742, 811, 893
RsaI GTAC 1 cut(s) 1508
RsaNI GTAC 1 cut(s) 1507
RseI CAYNNNNRTG 1 cut(s) 1292
SacI GAGCTC 1 cut(s) 519
Sau3AI GATC 6 cut(s) 252, 423, 693, 726, 903, 1311
Sau96I GGNCC 8 cut(s) 375, 396, 412, 477, 562, 745, 928, 1156
ScaI AGTACT 1 cut(s) 1508
SchI GAGTC 3 cut(s) 344, 422, 1534
ScrFI CCNGG 6 cut(s) 373, 590, 691, 716, 932, 951
SduI GDGCHC 3 cut(s) 519, 1048, 1370
SfaNI GCATC 2 cut(s) 546, 1231
SfcI CTRYAG 1 cut(s) 908
SfoI GGCGCC 1 cut(s) 766
SfuI TTCGAA 1 cut(s) 453
SgrAI CRCCGGYG 2 cut(s) 647, 1456
SinI GGWCC 6 cut(s) 396, 412, 562, 745, 928, 1156
SmiMI CAYNNNNRTG 1 cut(s) 1292
SmlI CTYRAG 3 cut(s) 875, 1047, 1510
SmoI CTYRAG 3 cut(s) 875, 1047, 1510
SspDI GGCGCC 1 cut(s) 764
SspMI CTAG 1 cut(s) 1037
SstI GAGCTC 1 cut(s) 519
StyD4I CCNGG 6 cut(s) 371, 588, 689, 714, 930, 949
TaaI ACNGT 4 cut(s) 94, 577, 866, 1357
TaiI ACGT 3 cut(s) 192, 224, 291
TaqI TCGA 8 cut(s) 246, 285, 426, 453, 795, 918, 1166, 1262
TatI WGTACW 1 cut(s) 1506
TauI GCSGC 7 cut(s) 123, 279, 330, 537, 665, 686, 1307
TfiI GAWTC 6 cut(s) 233, 1027, 1063, 1259, 1423, 1534
TscAI CASTG 2 cut(s) 536, 1294
TseFI GTSAC 1 cut(s) 823
TseI GCWGC 7 cut(s) 686, 739, 808, 835, 893, 910, 1052
Tsp45I GTSAC 1 cut(s) 823
TspDTI ATGAA 2 cut(s) 524, 626
TspGWI ACGGA 1 cut(s) 721
TspRI CASTG 2 cut(s) 536, 1294
Tth111I GACNNNGTC 1 cut(s) 440
VpaK11BI GGWCC 6 cut(s) 396, 412, 562, 745, 928, 1156
XceI RCATGY 3 cut(s) 304, 1000, 1273
XmiI GTMKAC 1 cut(s) 258
XspI CTAG 1 cut(s) 1037
ZraI GACGTC 1 cut(s) 289
ZrmI AGTACT 1 cut(s) 1508
Zsp2I ATGCAT 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.