Rh2AG440200

Threonine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005631
Physical Location & Seq
Reverse (-)
65088292 .. 65090458
2167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG440200.1

Sequence Viewer

Length: 1536 bp
ATGGCGTCTTCCTCTCTCTTCCAATCCCCCTCCTTCTCTCTCAAACCCCAAAAGCCCATTTCCCCGCCCTCCACCAAACCCCATTTCACCACCACCATCAAATGCTCCTCCGCCGCCAAATGCCGCGACAACATCCGCGACGACGCCCACCGCCACAACACCACCCACACCCACCACTTCTCCGCCAAGTACGTCCCCTTCAACTCCTCCGCAAACGACACGTCGTCCGAGTCCTACTCCCTCGACGAGGTCGTCTACCGCTCCCAGTCCGGCGGCCTCCTCGACGTCCAGCACGACATGGCCGCCCTCAAGAACTACGACGGCAAGTACTGGCGCGACCTCTTCGACTCCCGCGTCGGCAAAACCACCTGGCCCTACGGCTCCGGCGTCTGGTCCAAAAAGGAGTGGGTCCTCCCTGAGATCGACAGCGACGACATCGTCTCCGCCTTCGAGGGCAACTCCAACCTCTTCTGGGCCGAGCGCTACGGCAAAACAGTCCTCGGCATGAACGAGCTCTGGGTCAAGCACTGCGGCATCAGCCACACCGGCTCCTTCAAGGACCTAGGCATGACCGTCCTCGTCAGCCAGGTCAATCGCCTCCGCAAAATGAACCGCCCCGTCGTCGGCGTCGGATGCGCCTCCACCGGCGACACCTCCGCCGCCCTGTCCGCCTACTGCGCCTCCGCCGGGATCCCCTCCATTGTGTTTCTGCCGGCGAATAAGATCTCGATGGCCCAGCTGGTCCAGCCCATCGCAAACGGGGCCTTTGTGCTGAGCATCGACACCGATTTCGATGGCTGTATGAATCTGATTCGAGAAGTGACTGCAGAATTGCCGATTTATTTAGCTAATTCTCTGAACAGTTTGAGACTGGAGGGCCAGAAAACAGCAGCTATTGAGATTCTGCAGCAGTTTGATTGGCAGGTCCCGGATTGGGTTATAGTTCCGGGAGGCAATCTCGGAAATATATATGCATTCTACAAAGGGTTTCATATGTGTAAGGAATTGGGGCTGGTTGATAAGATTCCTAGACTTGTGTGTGCTCAAGCTGCAAATGCAAACCCTTTGTACTTGCATTACAAGTCGGGGTGGGATGAGGAGTTCAAGCCTGTGAAGGCAAACACTACATTTGCGTCTGCAATTCAGATTGGGGATCCTGTTTCCATTGACAGGGCCGTGTATGCGCTGAAGAAGTCGGATGGGATTGTGGAGGAGGCGACCGAGGAGGAGTTGATGGATGCAATGGCGCAAGCGGATTCTACCGGCATGTTTATATGTCCTCACACTGGTGTGGCGCTGGCAGCGTTGATCAAGCTCCGGAATAAGGGGGTTATAGGCCCGACGGAGAGGACTGTGGTGGTGAGCACGGCTCATGGGTTGAAGTTCACGCAGGCGAAGATTGATTATCACTCTAAGGAGATTCCGGATTTGGCTTGCCGGTTTGCTAACCCACCGGTTCAAGTGAGGGCAGATTTTGGGTCGGTTATGGATGTTTTGAAGAAGTACTTGAGTAAGTCGGAGGCTCCAAAGCATTAG

Protein Analysis

511

Amino Acids

56.12

Weight (kDa)

6.94

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 148 - 456 2.8e-52 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 251, 353, 437
AatII GACGTC 1 cut(s) 288
Acc36I ACCTGC 1 cut(s) 913
AccBSI CCGCTC 1 cut(s) 261
AccI GTMKAC 1 cut(s) 255
AccII CGCG 4 cut(s) 126, 138, 336, 354
AccIII TCCGGA 2 cut(s) 1317, 1423
AclWI GGATC 4 cut(s) 685, 698, 1148, 1161
AcoI YGGCCR 1 cut(s) 300
AcuI CTGAAG 1 cut(s) 1208
AcyI GRCGYC 5 cut(s) 5, 144, 285, 387, 627
AfaI GTAC 4 cut(s) 191, 329, 1070, 1505
AfeI AGCGCT 1 cut(s) 482
AfiI CCNNNNNNNGG 9 cut(s) 247, 390, 472, 623, 687, 934, 1170, 1286, 1324
AflIII ACRYGT 1 cut(s) 219
AgeI ACCGGT 1 cut(s) 1453
AgsI TTSAA 6 cut(s) 202, 556, 1105, 1381, 1460, 1498
AhdI GACNNNNNGTC 1 cut(s) 223
AjiI CACGTC 1 cut(s) 222
AjnI CCWGG 2 cut(s) 368, 585
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AleI CACNNNNGTG 2 cut(s) 1287, 1289
AloI GAACNNNNNNTCC 2 cut(s) 1085, 1117
AluBI AGCT 6 cut(s) 514, 739, 848, 893, 1049, 1315
AluI AGCT 6 cut(s) 514, 739, 848, 893, 1049, 1315
Alw21I GWGCWC 3 cut(s) 516, 1045, 1367
Alw26I GTCTC 2 cut(s) 445, 862
AlwI GGATC 4 cut(s) 685, 698, 1148, 1161
Aor13HI TCCGGA 2 cut(s) 1317, 1423
Aor51HI AGCGCT 1 cut(s) 482
AoxI GGCC 9 cut(s) 274, 300, 371, 474, 732, 762, 877, 1173, 1336
ApeKI GCWGC 4 cut(s) 890, 907, 1049, 1301
ArsI GACNNNNNNTTYG 4 cut(s) 206, 238, 773, 805
AsiGI ACCGGT 1 cut(s) 1453
AspA2I CCTAGG 1 cut(s) 562
AspLEI GCGC 7 cut(s) 336, 483, 638, 680, 1186, 1249, 1297
AsuC2I CCSGG 3 cut(s) 688, 929, 948
AsuHPI GGTGA 2 cut(s) 79, 1372
AvaII GGWCC 5 cut(s) 393, 409, 559, 742, 925
AvrII CCTAGG 1 cut(s) 562
BamHI GGATCC 2 cut(s) 690, 1153
BanII GRGCYC 1 cut(s) 516
BarI GAAGNNNNNNTAC 2 cut(s) 182, 214
Bbv12I GWGCWC 3 cut(s) 516, 1045, 1367
BbvI GCAGC 4 cut(s) 902, 919, 1036, 1313
BccI CCATC 6 cut(s) 104, 724, 758, 788, 1193, 1228
BceAI ACGGC 5 cut(s) 337, 394, 502, 1160, 1383
BciT130I CCWGG 2 cut(s) 370, 587
BclI TGATCA 1 cut(s) 1308
BcnI CCSGG 3 cut(s) 688, 929, 948
BcoDI GTCTC 2 cut(s) 445, 862
BfaI CTAG 2 cut(s) 563, 1029
BfmI CTRYAG 2 cut(s) 825, 905
BfoI RGCGCY 2 cut(s) 484, 1298
BfuAI ACCTGC 1 cut(s) 913
BglI GCCNNNNNGGC 1 cut(s) 546
BglII AGATCT 1 cut(s) 723
BlnI CCTAGG 1 cut(s) 562
BlpI GCTNAGC 1 cut(s) 773
BmcAI AGTACT 2 cut(s) 329, 1505
Bme1390I CCNGG 5 cut(s) 370, 587, 688, 929, 948
Bme18I GGWCC 5 cut(s) 393, 409, 559, 742, 925
BmeRI GACNNNNNGTC 1 cut(s) 223
BmgBI CACGTC 1 cut(s) 222
BmiI GGNNCC 8 cut(s) 382, 410, 550, 692, 763, 927, 1155, 1524
BmrFI CCNGG 5 cut(s) 370, 587, 688, 929, 948
BmrI ACTGGG 1 cut(s) 259
BmsI GCATC 4 cut(s) 543, 623, 786, 1228
BmuI ACTGGG 1 cut(s) 259
BplI GAGNNNNNCTC 8 cut(s) 221, 253, 443, 475, 942, 974, 1354, 1386
BpmI CTGGAG 1 cut(s) 893
Bpu1102I GCTNAGC 1 cut(s) 773
BpuEI CTTGAG 3 cut(s) 293, 1029, 1528
BpuMI CCSGG 3 cut(s) 688, 929, 948
BsaHI GRCGYC 5 cut(s) 5, 144, 285, 387, 627
BsaJI CCNNGG 3 cut(s) 499, 562, 1221
BsaWI WCCGGW 3 cut(s) 1317, 1423, 1453
BsaXI ACNNNNNCTCC 8 cut(s) 164, 194, 425, 455, 533, 563, 665, 695
Bsc4I CCNNNNNNNGG 9 cut(s) 247, 390, 472, 623, 687, 934, 1170, 1286, 1324
Bse118I RCCGGY 6 cut(s) 545, 644, 712, 1262, 1437, 1453
Bse1I ACTGG 4 cut(s) 265, 335, 876, 1291
Bse3DI GCAATG 1 cut(s) 1248
BseAI TCCGGA 2 cut(s) 1317, 1423
BseBI CCWGG 2 cut(s) 370, 587
BseDI CCNNGG 3 cut(s) 499, 562, 1221
BseGI GGATG 6 cut(s) 132, 638, 1099, 1204, 1243, 1495
BseLI CCNNNNNNNGG 9 cut(s) 247, 390, 472, 623, 687, 934, 1170, 1286, 1324
BseMI GCAATG 1 cut(s) 1248
BseMII CTCAG 2 cut(s) 408, 764
BseNI ACTGG 4 cut(s) 265, 335, 876, 1291
BseRI GAGGAG 7 cut(s) 97, 196, 269, 1112, 1226, 1238, 1241
BseXI GCAGC 4 cut(s) 902, 919, 1036, 1313
BseYI CCCAGC 1 cut(s) 735
Bsh1236I CGCG 4 cut(s) 126, 138, 336, 354
Bsh1285I CGRYCG 1 cut(s) 1221
BshFI GGCC 9 cut(s) 276, 302, 373, 476, 734, 764, 879, 1175, 1338
BshTI ACCGGT 1 cut(s) 1453
BsiEI CGRYCG 1 cut(s) 1221
BsiHKAI GWGCWC 3 cut(s) 516, 1045, 1367
BslFI GGGAC 2 cut(s) 179, 911
BslI CCNNNNNNNGG 9 cut(s) 247, 390, 472, 623, 687, 934, 1170, 1286, 1324
BsmAI GTCTC 2 cut(s) 445, 862
BsmBI CGTCTC 1 cut(s) 445
BsmFI GGGAC 2 cut(s) 179, 911
BsmI GAATGC 1 cut(s) 974
BsnI GGCC 9 cut(s) 276, 302, 373, 476, 734, 764, 879, 1175, 1338
Bsp1286I GDGCHC 3 cut(s) 516, 1045, 1367
Bsp13I TCCGGA 2 cut(s) 1317, 1423
Bsp143I GATC 5 cut(s) 420, 690, 723, 1153, 1308
Bsp1720I GCTNAGC 1 cut(s) 773
BspANI GGCC 9 cut(s) 276, 302, 373, 476, 734, 764, 879, 1175, 1338
BspCNI CTCAG 2 cut(s) 409, 765
BspEI TCCGGA 2 cut(s) 1317, 1423
BspFNI CGCG 4 cut(s) 126, 138, 336, 354
BspLI GGNNCC 8 cut(s) 382, 410, 550, 692, 763, 927, 1155, 1524
BspMAI CTGCAG 2 cut(s) 829, 909
BspMI ACCTGC 1 cut(s) 913
BspPI GGATC 4 cut(s) 685, 698, 1148, 1161
BsrBI CCGCTC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 1248
BsrFI RCCGGY 6 cut(s) 545, 644, 712, 1262, 1437, 1453
BsrI ACTGG 4 cut(s) 265, 335, 876, 1291
BssAI RCCGGY 6 cut(s) 545, 644, 712, 1262, 1437, 1453
BssECI CCNNGG 3 cut(s) 499, 562, 1221
BssMI GATC 5 cut(s) 420, 690, 723, 1153, 1308
BssNI GRCGYC 5 cut(s) 5, 144, 285, 387, 627
BssT1I CCWWGG 1 cut(s) 562
Bst2UI CCWGG 2 cut(s) 370, 587
Bst4CI ACNGT 4 cut(s) 496, 574, 863, 1354
Bst6I CTCTTC 3 cut(s) 23, 347, 473
BstACI GRCGYC 5 cut(s) 5, 144, 285, 387, 627
BstC8I GCNNGC 5 cut(s) 714, 1251, 1299, 1392, 1435
BstDEI CTNAG 3 cut(s) 417, 773, 1413
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 6 cut(s) 132, 638, 1099, 1204, 1243, 1495
BstFNI CGCG 4 cut(s) 126, 138, 336, 354
BstH2I RGCGCY 2 cut(s) 484, 1298
BstHHI GCGC 7 cut(s) 336, 483, 638, 680, 1186, 1249, 1297
BstKTI GATC 5 cut(s) 423, 693, 726, 1156, 1311
BstMAI GTCTC 2 cut(s) 445, 862
BstMBI GATC 5 cut(s) 420, 690, 723, 1153, 1308
BstMCI CGRYCG 1 cut(s) 1221
BstNI CCWGG 2 cut(s) 370, 587
BstNSI RCATGY 1 cut(s) 1270
BstSCI CCNGG 5 cut(s) 368, 585, 686, 927, 946
BstSFI CTRYAG 2 cut(s) 825, 905
BstUI CGCG 4 cut(s) 126, 138, 336, 354
BstV1I GCAGC 4 cut(s) 902, 919, 1036, 1313
BstX2I RGATCY 3 cut(s) 690, 723, 1153
BstYI RGATCY 3 cut(s) 690, 723, 1153
BsuRI GGCC 9 cut(s) 276, 302, 373, 476, 734, 764, 879, 1175, 1338
BtgZI GCGATG 1 cut(s) 736
BtrI CACGTC 1 cut(s) 222
BtsCI GGATG 6 cut(s) 132, 638, 1099, 1204, 1243, 1495
BtsI GCAGTG 1 cut(s) 526
BtsIMutI CAGTG 2 cut(s) 526, 1284
BveI ACCTGC 1 cut(s) 913
Cac8I GCNNGC 5 cut(s) 714, 1251, 1299, 1392, 1435
CfoI GCGC 7 cut(s) 336, 483, 638, 680, 1186, 1249, 1297
Cfr10I RCCGGY 6 cut(s) 545, 644, 712, 1262, 1437, 1453
CseI GACGC 5 cut(s) 152, 343, 376, 616, 1122
Csp6I GTAC 4 cut(s) 190, 328, 1069, 1504
CspAI ACCGGT 1 cut(s) 1453
CviAII CATG 5 cut(s) 298, 505, 568, 1267, 1373
CviQI GTAC 4 cut(s) 190, 328, 1069, 1504
DdeI CTNAG 3 cut(s) 417, 773, 1413
DpnI GATC 5 cut(s) 422, 692, 725, 1155, 1310
DpnII GATC 5 cut(s) 420, 690, 723, 1153, 1308
DrdI GACNNNNNNGTC 3 cut(s) 251, 353, 437
DriI GACNNNNNGTC 1 cut(s) 223
DseDI GACNNNNNNGTC 3 cut(s) 251, 353, 437
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 3 cut(s) 23, 347, 473
Eam1105I GACNNNNNGTC 1 cut(s) 223
EarI CTCTTC 3 cut(s) 23, 347, 473
EciI GGCGGA 6 cut(s) 100, 172, 433, 646, 658, 673
Ecl136II GAGCTC 1 cut(s) 514
Eco130I CCWWGG 1 cut(s) 562
Eco24I GRGCYC 1 cut(s) 516
Eco47I GGWCC 5 cut(s) 393, 409, 559, 742, 925
Eco47III AGCGCT 1 cut(s) 482
Eco53kI GAGCTC 1 cut(s) 514
Eco57I CTGAAG 1 cut(s) 1208
EcoICRI GAGCTC 1 cut(s) 514
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoO109I RGGNCCY 4 cut(s) 409, 559, 762, 925
EcoRII CCWGG 2 cut(s) 368, 585
EcoT14I CCWWGG 1 cut(s) 562
EcoT22I ATGCAT 1 cut(s) 976
EcoT38I GRGCYC 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 562
Esp3I CGTCTC 1 cut(s) 445
FaeI CATG 5 cut(s) 301, 508, 571, 1270, 1376
FalI AAGNNNNNCTT 2 cut(s) 1490, 1522
FaqI GGGAC 2 cut(s) 179, 911
FatI CATG 5 cut(s) 297, 504, 567, 1266, 1372
FauI CCCGC 2 cut(s) 72, 359
FauNDI CATATG 1 cut(s) 993
FbaI TGATCA 1 cut(s) 1308
FblI GTMKAC 1 cut(s) 255
FokI GGATG 6 cut(s) 119, 645, 1106, 1211, 1250, 1502
FriOI GRGCYC 1 cut(s) 516
FspBI CTAG 2 cut(s) 563, 1029
GlaI GCGC 7 cut(s) 335, 482, 637, 679, 1185, 1248, 1296
GsaI CCCAGC 1 cut(s) 739
GsuI CTGGAG 1 cut(s) 893
HaeII RGCGCY 2 cut(s) 484, 1298
HaeIII GGCC 9 cut(s) 276, 302, 373, 476, 734, 764, 879, 1175, 1338
HgaI GACGC 5 cut(s) 152, 343, 376, 616, 1122
HhaI GCGC 7 cut(s) 336, 483, 638, 680, 1186, 1249, 1297
Hin1I GRCGYC 5 cut(s) 5, 144, 285, 387, 627
Hin1II CATG 5 cut(s) 301, 508, 571, 1270, 1376
Hin6I GCGC 7 cut(s) 334, 481, 636, 678, 1184, 1247, 1295
HinP1I GCGC 7 cut(s) 334, 481, 636, 678, 1184, 1247, 1295
HinfI GANTC 8 cut(s) 230, 347, 805, 811, 901, 1024, 1256, 1420
HphI GGTGA 2 cut(s) 79, 1372
Hpy166II GTNNAC 2 cut(s) 256, 1386
Hpy188I TCNGA 8 cut(s) 229, 632, 810, 858, 962, 1146, 1198, 1519
Hpy188III TCNNGA 5 cut(s) 310, 727, 815, 1318, 1424
Hpy8I GTNNAC 2 cut(s) 256, 1386
HpyAV CCTTC 5 cut(s) 43, 208, 457, 562, 1108
HpyCH4III ACNGT 4 cut(s) 496, 574, 863, 1354
HpyCH4IV ACGT 3 cut(s) 192, 221, 285
HpyCH4V TGCA 8 cut(s) 827, 907, 974, 1052, 1058, 1075, 1139, 1241
HpyF3I CTNAG 3 cut(s) 417, 773, 1413
HpySE526I ACGT 3 cut(s) 192, 221, 285
Hsp92I GRCGYC 5 cut(s) 5, 144, 285, 387, 627
Hsp92II CATG 5 cut(s) 301, 508, 571, 1270, 1376
HspAI GCGC 7 cut(s) 334, 481, 636, 678, 1184, 1247, 1295
Kpn2I TCCGGA 2 cut(s) 1317, 1423
KroI GCCGGC 1 cut(s) 712
KroNI GCCGGC 1 cut(s) 714
Ksp22I TGATCA 1 cut(s) 1308
Kzo9I GATC 5 cut(s) 420, 690, 723, 1153, 1308
LmnI GCTCC 6 cut(s) 110, 266, 386, 554, 1320, 1528
Lsp1109I GCAGC 4 cut(s) 902, 919, 1036, 1313
LweI GCATC 4 cut(s) 543, 623, 786, 1228
MaeI CTAG 2 cut(s) 563, 1029
MaeII ACGT 3 cut(s) 192, 221, 285
MaeIII GTNAC 1 cut(s) 820
MalI GATC 5 cut(s) 422, 692, 725, 1155, 1310
MbiI CCGCTC 1 cut(s) 261
MboI GATC 5 cut(s) 420, 690, 723, 1153, 1308
MboII GAAGA 6 cut(s) 10, 334, 460, 1201, 1408, 1510
MflI RGATCY 3 cut(s) 690, 723, 1153
MhlI GDGCHC 3 cut(s) 516, 1045, 1367
MluCI AATT 4 cut(s) 830, 850, 1004, 1140
MlyI GAGTC 2 cut(s) 239, 341
MmeI TCCRAC 4 cut(s) 486, 610, 1176, 1497
Mph1103I ATGCAT 1 cut(s) 976
MroI TCCGGA 2 cut(s) 1317, 1423
MroNI GCCGGC 1 cut(s) 712
MslI CAYNNNNRTG 2 cut(s) 1287, 1289
MspA1I CMGCKG 1 cut(s) 739
MspR9I CCNGG 5 cut(s) 370, 587, 688, 929, 948
Mva1269I GAATGC 1 cut(s) 974
MvaI CCWGG 2 cut(s) 370, 587
MvnI CGCG 4 cut(s) 126, 138, 336, 354
NaeI GCCGGC 1 cut(s) 714
NciI CCSGG 3 cut(s) 688, 929, 948
NdeI CATATG 1 cut(s) 993
NdeII GATC 5 cut(s) 420, 690, 723, 1153, 1308
NgoMIV GCCGGC 1 cut(s) 712
NlaIII CATG 5 cut(s) 301, 508, 571, 1270, 1376
NlaIV GGNNCC 8 cut(s) 382, 410, 550, 692, 763, 927, 1155, 1524
NmeAIII GCCGAG 2 cut(s) 480, 502
NmuCI GTSAC 1 cut(s) 820
NsiI ATGCAT 1 cut(s) 976
NspI RCATGY 1 cut(s) 1270
OliI CACNNNNGTG 2 cut(s) 1287, 1289
PcsI WCGNNNNNNNCGW 6 cut(s) 249, 291, 351, 384, 429, 507
PctI GAATGC 1 cut(s) 974
PdiI GCCGGC 1 cut(s) 714
PfeI GAWTC 6 cut(s) 805, 811, 901, 1024, 1256, 1420
PflFI GACNNNGTC 2 cut(s) 248, 437
PfoI TCCNGGA 2 cut(s) 927, 946
PinAI ACCGGT 1 cut(s) 1453
PleI GAGTC 2 cut(s) 238, 341
PpsI GAGTC 2 cut(s) 238, 341
PpuMI RGGWCCY 3 cut(s) 409, 559, 925
Psp124BI GAGCTC 1 cut(s) 516
Psp5II RGGWCCY 3 cut(s) 409, 559, 925
Psp6I CCWGG 2 cut(s) 368, 585
PspFI CCCAGC 1 cut(s) 735
PspGI CCWGG 2 cut(s) 368, 585
PspN4I GGNNCC 8 cut(s) 382, 410, 550, 692, 763, 927, 1155, 1524
PspPPI RGGWCCY 3 cut(s) 409, 559, 925
PstI CTGCAG 2 cut(s) 829, 909
PsuI RGATCY 3 cut(s) 690, 723, 1153
PsyI GACNNNGTC 2 cut(s) 248, 437
PvuII CAGCTG 1 cut(s) 739
RsaI GTAC 4 cut(s) 191, 329, 1070, 1505
RsaNI GTAC 4 cut(s) 190, 328, 1069, 1504
RseI CAYNNNNRTG 2 cut(s) 1287, 1289
SacI GAGCTC 1 cut(s) 516
Sau3AI GATC 5 cut(s) 420, 690, 723, 1153, 1308
ScaI AGTACT 2 cut(s) 329, 1505
SchI GAGTC 2 cut(s) 239, 341
ScrFI CCNGG 5 cut(s) 370, 587, 688, 929, 948
SduI GDGCHC 3 cut(s) 516, 1045, 1367
SfaNI GCATC 4 cut(s) 543, 623, 786, 1228
SfcI CTRYAG 2 cut(s) 825, 905
SgrAI CRCCGGYG 1 cut(s) 644
SinI GGWCC 5 cut(s) 393, 409, 559, 742, 925
SmiMI CAYNNNNRTG 2 cut(s) 1287, 1289
SmlI CTYRAG 3 cut(s) 308, 1044, 1507
SmoI CTYRAG 3 cut(s) 308, 1044, 1507
Sse9I AATT 4 cut(s) 830, 850, 1004, 1140
SspMI CTAG 2 cut(s) 563, 1029
SstI GAGCTC 1 cut(s) 516
StyD4I CCNGG 5 cut(s) 368, 585, 686, 927, 946
StyI CCWWGG 1 cut(s) 562
TaaI ACNGT 4 cut(s) 496, 574, 863, 1354
TaiI ACGT 3 cut(s) 195, 224, 288
TaqI TCGA 9 cut(s) 243, 282, 345, 423, 450, 728, 780, 792, 814
TaqII GACCGA 1 cut(s) 1235
TasI AATT 4 cut(s) 830, 850, 1004, 1140
TatI WGTACW 3 cut(s) 327, 1068, 1503
TauI GCSGC 6 cut(s) 116, 126, 276, 305, 534, 662
TfiI GAWTC 6 cut(s) 805, 811, 901, 1024, 1256, 1420
TscAI CASTG 2 cut(s) 533, 1291
TseFI GTSAC 1 cut(s) 820
TseI GCWGC 4 cut(s) 890, 907, 1049, 1301
Tsp45I GTSAC 1 cut(s) 820
TspDTI ATGAA 4 cut(s) 521, 623, 818, 980
TspGWI ACGGA 1 cut(s) 1358
TspRI CASTG 2 cut(s) 533, 1291
Tth111I GACNNNGTC 2 cut(s) 248, 437
VpaK11BI GGWCC 5 cut(s) 393, 409, 559, 742, 925
XagI CCTNNNNNAGG 1 cut(s) 245
XceI RCATGY 1 cut(s) 1270
XmaJI CCTAGG 1 cut(s) 562
XmiI GTMKAC 1 cut(s) 255
XspI CTAG 2 cut(s) 563, 1029
ZraI GACGTC 1 cut(s) 286
ZrmI AGTACT 2 cut(s) 329, 1505
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.