MD09G1187000.v1.1

Threonine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005631
Physical Location & Seq
Forward (+)
16293785 .. 16295471
1687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1187000.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGGCATCTTCCTCTCTCTTCCAATCTCCCCCTCTCTCCCTCAAACCCCAAAACCCCGCTTCCCACCACGCTCCAACCAAACCCCAATTCGCCACCACCATCAAATGCGTCTCCACCTCCACCCCTCCTCCCTACTCCTCCTCCGACCACCCTCACCACAACATTCGCGACGAGGCTCGCCGCCACAACACCACCCACTCCCACCACTTCTCCGCCCGTTACGTTCCCTTCAACGCCCACCCATCTTCCGCCGCCGCCGCCGAGTCCTACTCCCTCGACGAGATCGTCTACCGATCCAACTCCGGCGGCCTGCTCGACGTCCAGCACGATATGGCCGCCCTCAAAAACTACGACGGAAAGTACTGGCGAGACCTCTTCGACTCCCGCGTCGGCAAGACCACCTGGCCCTACGGCTCCGGCGTCTGGAGTAAGAAGGAGTGGGTCCTACCCGAGATCGACAGCGACGACATTGTCTCCGCATTTGAAGGTAACTCCAATCTCTTCTGGGCTGAGCGTTATGGCAAACAGATTCTGGCCATGAACGATTTGTGGGTCAAACACTGTGGGATCAGCCACACTGGAAGTTTTAAGGACTTGGGTATGACTGTTTTGGTCAGCCAAGTGAACCGGCTGAGGAAAATGAACCGCCCAGTGGTCGGCGTGGGCTGTGCCTCCACTGGAGACACATCCGCTGCCTTATCAGCCTACTGCGCCGCAGCTGGGATACCCTCGATTGTGTTTCTACCAGCGAATCGAATATCTCTGGCTCAGCTGGTTCAGCCCATTGCCAATGGGGCCTTTGTGCTGAGCATTGATACTGATTTCGATGGGTGTATGCAGTTGATTCGAGAAGTCACAGCGGAGCTGCCGATTTATTTGGCTAATTCTTTGAACAGTTTGAGATTAGAGGGTCAGAAAACCGCTGCAATTGAGATTTTGCAGCAGTTTGATTGGGAAGTGCCGGATTGGGTCATAGTTCCAGGGGGAAATTTGGGTAACATATATGCTTTCTACAAAGGATTTCATATGTGCAAAGAGTTGGGACTGGTCGATAGGATTCCTAGGCTCGTTTGTGCCCAAGCTGCAAATGCGAACCCGCTGTACTTGCATTACAAGTCAGGGTGGGATCAAGAGTTCAAGCCCGTGAAGGCGAACACTACGTTTGCATCCGCAATTCAAATAGGCGATCCTGTTTCGATAGACAGAGCCGTGTATGCGCTGAAGAAGTCGAATGGGATTGTGGAGGAAGCCACTGAGCAGGAGTTGATGGATGCCATGTCTCAAGCAGACTCCACTGGCATGTTCATATGCCCTCACACTGGTGTGGCATTGGCGGCATTGATTAAGCTTCGGAACCAAGGGATCATAGGCACTAACGACCGGACTGTGGTGGTGAGCACGGCTCACGGGCTGAAATTCACGCAGTCGAAGATTGATTATCATTCAAATGATATCAAGGATTTGGCTTGCAAGTATGCTAACCCGCCGGTGCAAGTGAAGGCGGATTTTGGGTCCGTGATGGATGTTTTGAAGAAGTACTTGTTGAGCAAGGCACCGAAGAATTAG

Protein Analysis

522

Amino Acids

57.18

Weight (kDa)

7.28

Isoelectric Point (pI)

32.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 159 - 467 3.1e-52 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 284, 386, 470
AatII GACGTC 1 cut(s) 321
AccB1I GGYRCC 1 cut(s) 1552
AccI GTMKAC 1 cut(s) 288
AccII CGCG 2 cut(s) 168, 387
AclWI GGATC 5 cut(s) 288, 575, 1134, 1181, 1370
AcoI YGGCCR 2 cut(s) 333, 534
AcsI RAATTY 2 cut(s) 988, 1415
AcuI CTGAAG 1 cut(s) 1241
AcyI GRCGYC 2 cut(s) 318, 420
AfaI GTAC 3 cut(s) 362, 1103, 1538
AfiI CCNNNNNNNGG 6 cut(s) 423, 652, 656, 720, 1319, 1387
AgsI TTSAA 7 cut(s) 232, 485, 892, 1138, 1178, 1446, 1531
AjnI CCWGG 2 cut(s) 401, 979
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AleI CACNNNNGTG 2 cut(s) 1320, 1322
AloI GAACNNNNNNTCC 2 cut(s) 1118, 1150
AluBI AGCT 5 cut(s) 719, 772, 865, 1082, 1348
AluI AGCT 5 cut(s) 719, 772, 865, 1082, 1348
Alw21I GWGCWC 1 cut(s) 1400
Alw26I GTCTC 5 cut(s) 115, 363, 478, 675, 1284
AlwI GGATC 5 cut(s) 288, 575, 1134, 1181, 1370
AlwNI CAGNNNCTG 1 cut(s) 532
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 5 cut(s) 307, 333, 404, 534, 795
ApeKI GCWGC 6 cut(s) 692, 716, 865, 923, 940, 1082
ApoI RAATTY 2 cut(s) 988, 1415
AspA2I CCTAGG 1 cut(s) 1061
AspLEI GCGC 2 cut(s) 713, 1219
AspS9I GGNCC 4 cut(s) 405, 442, 795, 1512
AsuHPI GGTGA 2 cut(s) 146, 1405
AvaI CYCGRG 1 cut(s) 449
AvaII GGWCC 2 cut(s) 442, 1512
AvrII CCTAGG 1 cut(s) 1061
BaeGI GKGCMC 1 cut(s) 1078
BalI TGGCCA 1 cut(s) 536
BanI GGYRCC 1 cut(s) 1552
Bbv12I GWGCWC 1 cut(s) 1400
BbvCI CCTCAGC 1 cut(s) 632
BbvI GCAGC 6 cut(s) 679, 728, 852, 910, 952, 1069
BccI CCATC 5 cut(s) 107, 250, 821, 1261, 1513
BceAI ACGGC 3 cut(s) 427, 1193, 1416
BcgI CGANNNNNNTGC 2 cut(s) 827, 861
BciT130I CCWGG 2 cut(s) 403, 981
BciVI GTATCC 1 cut(s) 717
BcoDI GTCTC 5 cut(s) 115, 363, 478, 675, 1284
BfaI CTAG 1 cut(s) 1062
BfuI GTATCC 1 cut(s) 717
BglI GCCNNNNNGGC 1 cut(s) 794
BlnI CCTAGG 1 cut(s) 1061
BlpI GCTNAGC 3 cut(s) 510, 768, 806
BmcAI AGTACT 2 cut(s) 362, 1538
Bme1390I CCNGG 2 cut(s) 403, 981
Bme18I GGWCC 2 cut(s) 442, 1512
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 4 cut(s) 405, 442, 795, 1512
BmiI GGNNCC 6 cut(s) 415, 443, 796, 1355, 1513, 1554
BmrFI CCNGG 2 cut(s) 403, 981
BmrI ACTGGG 1 cut(s) 644
BmsI GCATC 3 cut(s) 14, 1175, 1261
BmuI ACTGGG 1 cut(s) 644
BplI GAGNNNNNCTC 4 cut(s) 254, 286, 1387, 1419
BpmI CTGGAG 2 cut(s) 445, 699
Bpu10I CCTNAGC 1 cut(s) 632
Bpu1102I GCTNAGC 3 cut(s) 510, 768, 806
BpuEI CTTGAG 1 cut(s) 1266
BsaHI GRCGYC 2 cut(s) 318, 420
BsaI GGTCTC 1 cut(s) 363
BsaJI CCNNGG 3 cut(s) 980, 1061, 1357
BsaWI WCCGGW 1 cut(s) 1380
BsaXI ACNNNNNCTCC 8 cut(s) 112, 125, 142, 155, 194, 224, 458, 488
Bsc4I CCNNNNNNNGG 6 cut(s) 423, 652, 656, 720, 1319, 1387
Bse118I RCCGGY 2 cut(s) 627, 1486
Bse1I ACTGG 7 cut(s) 368, 583, 650, 682, 1050, 1300, 1324
Bse3DI GCAATG 1 cut(s) 783
BseBI CCWGG 2 cut(s) 403, 981
BseDI CCNNGG 3 cut(s) 980, 1061, 1357
BseGI GGATG 4 cut(s) 686, 1166, 1276, 1528
BseLI CCNNNNNNNGG 6 cut(s) 423, 652, 656, 720, 1319, 1387
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 5 cut(s) 501, 623, 782, 797, 1245
BseNI ACTGG 7 cut(s) 368, 583, 650, 682, 1050, 1300, 1324
BseRI GAGGAG 3 cut(s) 117, 127, 130
BseSI GKGCMC 1 cut(s) 1078
BseXI GCAGC 6 cut(s) 679, 728, 852, 910, 952, 1069
BseYI CCCAGC 1 cut(s) 719
Bsh1236I CGCG 2 cut(s) 168, 387
Bsh1285I CGRYCG 1 cut(s) 1381
BshFI GGCC 5 cut(s) 309, 335, 406, 536, 797
BshNI GGYRCC 1 cut(s) 1552
BsiEI CGRYCG 1 cut(s) 1381
BsiHKAI GWGCWC 1 cut(s) 1400
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 6 cut(s) 303, 417, 628, 962, 1381, 1487
BslFI GGGAC 1 cut(s) 1056
BslI CCNNNNNNNGG 6 cut(s) 423, 652, 656, 720, 1319, 1387
BsmAI GTCTC 5 cut(s) 115, 363, 478, 675, 1284
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 1056
BsnI GGCC 5 cut(s) 309, 335, 406, 536, 797
Bso31I GGTCTC 1 cut(s) 363
BsoBI CYCGRG 1 cut(s) 449
Bsp1286I GDGCHC 2 cut(s) 1078, 1400
Bsp143I GATC 7 cut(s) 282, 293, 453, 567, 1126, 1186, 1362
Bsp1720I GCTNAGC 3 cut(s) 510, 768, 806
Bsp68I TCGCGA 1 cut(s) 168
BspANI GGCC 5 cut(s) 309, 335, 406, 536, 797
BspCNI CTCAG 5 cut(s) 502, 624, 781, 798, 1246
BspFNI CGCG 2 cut(s) 168, 387
BspLI GGNNCC 6 cut(s) 415, 443, 796, 1355, 1513, 1554
BspPI GGATC 5 cut(s) 288, 575, 1134, 1181, 1370
BspT107I GGYRCC 1 cut(s) 1552
BspTNI GGTCTC 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 783
BsrFI RCCGGY 2 cut(s) 627, 1486
BsrI ACTGG 7 cut(s) 368, 583, 650, 682, 1050, 1300, 1324
BssAI RCCGGY 2 cut(s) 627, 1486
BssECI CCNNGG 3 cut(s) 980, 1061, 1357
BssMI GATC 7 cut(s) 282, 293, 453, 567, 1126, 1186, 1362
BssNI GRCGYC 2 cut(s) 318, 420
BssT1I CCWWGG 2 cut(s) 1061, 1357
Bst2UI CCWGG 2 cut(s) 403, 981
Bst4CI ACNGT 4 cut(s) 563, 607, 896, 1387
Bst6I CTCTTC 3 cut(s) 23, 380, 506
BstACI GRCGYC 2 cut(s) 318, 420
BstC8I GCNNGC 3 cut(s) 178, 311, 1468
BstDEI CTNAG 5 cut(s) 510, 632, 768, 806, 1254
BstF5I GGATG 4 cut(s) 686, 1166, 1276, 1528
BstFNI CGCG 2 cut(s) 168, 387
BstHHI GCGC 2 cut(s) 713, 1219
BstKTI GATC 7 cut(s) 285, 296, 456, 570, 1129, 1189, 1365
BstMAI GTCTC 5 cut(s) 115, 363, 478, 675, 1284
BstMBI GATC 7 cut(s) 282, 293, 453, 567, 1126, 1186, 1362
BstMCI CGRYCG 1 cut(s) 1381
BstNI CCWGG 2 cut(s) 403, 981
BstNSI RCATGY 1 cut(s) 1303
BstSCI CCNGG 2 cut(s) 401, 979
BstSLI GKGCMC 1 cut(s) 1078
BstUI CGCG 2 cut(s) 168, 387
BstV1I GCAGC 6 cut(s) 679, 728, 852, 910, 952, 1069
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 5 cut(s) 309, 335, 406, 536, 797
BtsCI GGATG 4 cut(s) 686, 1166, 1276, 1528
BtsIMutI CAGTG 7 cut(s) 559, 576, 657, 675, 1251, 1293, 1317
BtuMI TCGCGA 1 cut(s) 168
Cac8I GCNNGC 3 cut(s) 178, 311, 1468
CaiI CAGNNNCTG 1 cut(s) 532
CfoI GCGC 2 cut(s) 713, 1219
Cfr10I RCCGGY 2 cut(s) 627, 1486
Cfr13I GGNCC 4 cut(s) 405, 442, 795, 1512
CseI GACGC 3 cut(s) 97, 376, 409
Csp6I GTAC 3 cut(s) 361, 1102, 1537
CspCI CAANNNNNGTGG 2 cut(s) 544, 579
CviAII CATG 3 cut(s) 538, 1276, 1300
CviQI GTAC 3 cut(s) 361, 1102, 1537
DdeI CTNAG 5 cut(s) 510, 632, 768, 806, 1254
DpnI GATC 7 cut(s) 284, 295, 455, 569, 1128, 1188, 1364
DpnII GATC 7 cut(s) 282, 293, 453, 567, 1126, 1186, 1362
DrdI GACNNNNNNGTC 3 cut(s) 284, 386, 470
DseDI GACNNNNNNGTC 3 cut(s) 284, 386, 470
EaeI YGGCCR 2 cut(s) 333, 534
Eam1104I CTCTTC 3 cut(s) 23, 380, 506
EarI CTCTTC 3 cut(s) 23, 380, 506
EciI GGCGGA 3 cut(s) 202, 238, 1517
Eco130I CCWWGG 2 cut(s) 1061, 1357
Eco31I GGTCTC 1 cut(s) 363
Eco32I GATATC 1 cut(s) 1453
Eco47I GGWCC 2 cut(s) 442, 1512
Eco57I CTGAAG 1 cut(s) 1241
Eco88I CYCGRG 1 cut(s) 449
EcoO109I RGGNCCY 2 cut(s) 442, 795
EcoRII CCWGG 2 cut(s) 401, 979
EcoRV GATATC 1 cut(s) 1453
EcoT14I CCWWGG 2 cut(s) 1061, 1357
ErhI CCWWGG 2 cut(s) 1061, 1357
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 3 cut(s) 541, 1279, 1303
FalI AAGNNNNNCTT 2 cut(s) 1523, 1555
FaqI GGGAC 1 cut(s) 1056
FatI CATG 3 cut(s) 537, 1275, 1299
FauI CCCGC 4 cut(s) 64, 392, 1104, 1491
FauNDI CATATG 2 cut(s) 1026, 1307
FblI GTMKAC 1 cut(s) 288
FokI GGATG 4 cut(s) 673, 1153, 1283, 1535
FspBI CTAG 1 cut(s) 1062
GlaI GCGC 2 cut(s) 712, 1218
GsaI CCCAGC 1 cut(s) 723
GsuI CTGGAG 2 cut(s) 445, 699
HaeIII GGCC 5 cut(s) 309, 335, 406, 536, 797
HapII CCGG 6 cut(s) 303, 417, 628, 962, 1381, 1487
HgaI GACGC 3 cut(s) 97, 376, 409
HhaI GCGC 2 cut(s) 713, 1219
Hin1I GRCGYC 2 cut(s) 318, 420
Hin1II CATG 3 cut(s) 541, 1279, 1303
Hin6I GCGC 2 cut(s) 711, 1217
HinP1I GCGC 2 cut(s) 711, 1217
HindIII AAGCTT 1 cut(s) 1346
HinfI GANTC 7 cut(s) 263, 380, 529, 751, 844, 1057, 1289
HpaII CCGG 6 cut(s) 303, 417, 628, 962, 1381, 1487
HphI GGTGA 2 cut(s) 146, 1405
Hpy166II GTNNAC 2 cut(s) 289, 625
Hpy188I TCNGA 3 cut(s) 145, 915, 1353
Hpy188III TCNNGA 4 cut(s) 167, 424, 848, 1130
Hpy8I GTNNAC 2 cut(s) 289, 625
Hpy99I CGWCG 6 cut(s) 173, 281, 320, 356, 392, 467
HpyAV CCTTC 5 cut(s) 238, 427, 479, 1141, 1492
HpyCH4III ACNGT 4 cut(s) 563, 607, 896, 1387
HpyCH4IV ACGT 3 cut(s) 222, 318, 1160
HpyCH4V TGCA 9 cut(s) 838, 926, 940, 1032, 1085, 1108, 1166, 1470, 1492
HpyF3I CTNAG 5 cut(s) 510, 632, 768, 806, 1254
HpySE526I ACGT 3 cut(s) 222, 318, 1160
Hsp92I GRCGYC 2 cut(s) 318, 420
Hsp92II CATG 3 cut(s) 541, 1279, 1303
HspAI GCGC 2 cut(s) 711, 1217
Kzo9I GATC 7 cut(s) 282, 293, 453, 567, 1126, 1186, 1362
LmnI GCTCC 3 cut(s) 76, 419, 862
Lsp1109I GCAGC 6 cut(s) 679, 728, 852, 910, 952, 1069
LweI GCATC 3 cut(s) 14, 1175, 1261
MaeI CTAG 1 cut(s) 1062
MaeII ACGT 3 cut(s) 222, 318, 1160
MaeIII GTNAC 4 cut(s) 218, 488, 853, 995
MalI GATC 7 cut(s) 284, 295, 455, 569, 1128, 1188, 1364
MboI GATC 7 cut(s) 282, 293, 453, 567, 1126, 1186, 1362
MboII GAAGA 7 cut(s) 10, 237, 367, 493, 1234, 1441, 1543
MfeI CAATTG 1 cut(s) 927
MhlI GDGCHC 2 cut(s) 1078, 1400
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 7 cut(s) 86, 883, 927, 988, 1173, 1415, 1561
MluNI TGGCCA 1 cut(s) 536
MlyI GAGTC 3 cut(s) 272, 374, 1283
MmeI TCCRAC 3 cut(s) 98, 168, 321
Mox20I TGGCCA 1 cut(s) 536
MscI TGGCCA 1 cut(s) 536
MseI TTAA 2 cut(s) 588, 1344
MslI CAYNNNNRTG 4 cut(s) 1298, 1320, 1322, 1446
Msp20I TGGCCA 1 cut(s) 536
MspA1I CMGCKG 6 cut(s) 692, 719, 772, 860, 923, 1099
MspI CCGG 6 cut(s) 303, 417, 628, 962, 1381, 1487
MspR9I CCNGG 2 cut(s) 403, 981
MunI CAATTG 1 cut(s) 927
MvaI CCWGG 2 cut(s) 403, 981
MvnI CGCG 2 cut(s) 168, 387
NdeI CATATG 2 cut(s) 1026, 1307
NdeII GATC 7 cut(s) 282, 293, 453, 567, 1126, 1186, 1362
NlaIII CATG 3 cut(s) 541, 1279, 1303
NlaIV GGNNCC 6 cut(s) 415, 443, 796, 1355, 1513, 1554
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 1 cut(s) 853
NruI TCGCGA 1 cut(s) 168
NspI RCATGY 1 cut(s) 1303
OliI CACNNNNGTG 2 cut(s) 1320, 1322
PcsI WCGNNNNNNNCGW 5 cut(s) 282, 324, 384, 417, 462
PfeI GAWTC 4 cut(s) 529, 751, 844, 1057
PflFI GACNNNGTC 1 cut(s) 470
PleI GAGTC 3 cut(s) 271, 374, 1283
PpsI GAGTC 3 cut(s) 271, 374, 1283
PpuMI RGGWCCY 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 2 cut(s) 401, 979
PspFI CCCAGC 1 cut(s) 719
PspGI CCWGG 2 cut(s) 401, 979
PspN4I GGNNCC 6 cut(s) 415, 443, 796, 1355, 1513, 1554
PspPI GGNCC 4 cut(s) 405, 442, 795, 1512
PspPPI RGGWCCY 1 cut(s) 442
PsrI GAACNNNNNNTAC 2 cut(s) 1085, 1117
PstNI CAGNNNCTG 1 cut(s) 532
PsyI GACNNNGTC 1 cut(s) 470
PvuII CAGCTG 2 cut(s) 719, 772
RruI TCGCGA 1 cut(s) 168
RsaI GTAC 3 cut(s) 362, 1103, 1538
RsaNI GTAC 3 cut(s) 361, 1102, 1537
RseI CAYNNNNRTG 4 cut(s) 1298, 1320, 1322, 1446
SaqAI TTAA 2 cut(s) 588, 1344
Sau3AI GATC 7 cut(s) 282, 293, 453, 567, 1126, 1186, 1362
Sau96I GGNCC 4 cut(s) 405, 442, 795, 1512
ScaI AGTACT 2 cut(s) 362, 1538
SchI GAGTC 3 cut(s) 272, 374, 1283
ScrFI CCNGG 2 cut(s) 403, 981
SduI GDGCHC 2 cut(s) 1078, 1400
SfaNI GCATC 3 cut(s) 14, 1175, 1261
SgrAI CRCCGGYG 1 cut(s) 1486
SinI GGWCC 2 cut(s) 442, 1512
SmiMI CAYNNNNRTG 4 cut(s) 1298, 1320, 1322, 1446
SmlI CTYRAG 1 cut(s) 1281
SmoI CTYRAG 1 cut(s) 1281
Sse9I AATT 7 cut(s) 86, 883, 927, 988, 1173, 1415, 1561
SspMI CTAG 1 cut(s) 1062
StyD4I CCNGG 2 cut(s) 401, 979
StyI CCWWGG 2 cut(s) 1061, 1357
TaaI ACNGT 4 cut(s) 563, 607, 896, 1387
TaiI ACGT 3 cut(s) 225, 321, 1163
TasI AATT 7 cut(s) 86, 883, 927, 988, 1173, 1415, 1561
TatI WGTACW 3 cut(s) 360, 1101, 1536
TauI GCSGC 8 cut(s) 183, 254, 257, 260, 309, 338, 716, 1337
TfiI GAWTC 4 cut(s) 529, 751, 844, 1057
Tru1I TTAA 2 cut(s) 588, 1344
Tru9I TTAA 2 cut(s) 588, 1344
TscAI CASTG 7 cut(s) 566, 583, 657, 682, 1258, 1300, 1324
TseFI GTSAC 1 cut(s) 853
TseI GCWGC 6 cut(s) 692, 716, 865, 923, 940, 1082
Tsp45I GTSAC 1 cut(s) 853
TspDTI ATGAA 4 cut(s) 554, 656, 1013, 1294
TspGWI ACGGA 2 cut(s) 369, 1504
TspRI CASTG 7 cut(s) 566, 583, 657, 682, 1258, 1300, 1324
Tth111I GACNNNGTC 1 cut(s) 470
VpaK11BI GGWCC 2 cut(s) 442, 1512
XapI RAATTY 2 cut(s) 988, 1415
XceI RCATGY 1 cut(s) 1303
XmaJI CCTAGG 1 cut(s) 1061
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 1 cut(s) 1062
ZraI GACGTC 1 cut(s) 319
ZrmI AGTACT 2 cut(s) 362, 1538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.