RLG00000020095

Threonine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005631
Physical Location & Seq
Reverse (-)
60943593 .. 60945146
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020095

Sequence Viewer

Length: 1554 bp
ATGGCGTCCTCCTCTCTCTTCCAATCCCCCTCCTTCTCTCTCAAACCCCAAAAGCCCTTTTCCCCACCCTCCGCCAAACCCCATTTCACCACCATCAAATGCTCCTCCTCCTCCTCCGCCGCCAAATGCCGCGACAACATCCGCGACGACGCCTACCGCCACAACACCACCCACACCCACCACTTCTCCGCCAAGTACGTCCCCTTCAACTCCTCCGCAAACGACACGTCGTCCGAGTCCGAGTCCGAGTCCTACTCCCTCGACGAGGTCGTGTACCGCTCCCAGTCCGGCGGCCTCCTCGACGTCCAGCACGACATGGCCGCCCTCAAGAACTACGACGGCAAGTACTGGCGCGACCTCTTCGACTCCCGCGTCGGCAAAACCACCTGGCCCTACGGCTCCGGCGTCTGGTCCAAAAAGGAGTGGGTCCTCCCTGAGATCGACAGCGACGACATCGTCTCCGCCTTCGAAGGCAACTCCAACCTCTTCTGGGCCGAGCGCTACGGCAAAACAGTCCTCGGCATGAACGAGCTCTGGGTCAAGCACTGCGGCATCAGCCACACCGGCTCCTTCAAGGACCTAGGCATGACCGTCCTCGTCAGCCAGGTCAATCGCCTCCGCAAAATGAACCGCCCCGTCGTCGGCGTCGGATGCGCCTCCACCGGCGACACCTCCGCCGCCTTGTCCGCCTACTGCGCCTCCGCCGGGATCCCCTCCATTGTGTTTCTGCCGGCGAATAAGATCTCGATGGCCCAGCTGGTCCAGCCCATCGCAAATGGGGCTTTTGTGCTGAGCATCGACACCGATTTCGATGGCTGTATGAATCTGATTCGAGAAGTGACTGCAGAATTGCCTATTTATTTAGCTAATTCTCTCAACAGTTTGAGACTGGAGGGCCAGAAAACAGCTGCTATTGAGATTCTGCAGCAGTTTGATTGGCAAGTCCCGGATTGGGTTATAGTTCCGGGAGGCAATCTCGGAAATATATATGCATTCTACAAAGGGTTTCATATGTGTAAGGAATTGGGGCTGGTTGACAAGATTCCTAGACTTGTGTGTGCTCAAGCTGCAAATGCAAACCCTTTGTACTTGCATTACAAGTCGGGGTGGGATGAGGAGTTCAAGCCTGTGAAGGCAAACACTACATTTGCGTCTGCAATTCAGATTGGGGATCCTGTTTCCATTGACAGGGCCGTGTATGCGCTGAAGAAGTCGGATGGGATTGTGGAGGAGGCGACCGAGGAGGAGTTGATGGATGCAATGGCGCAAGCGGATTCTACTGGCATGTTTATATGTCCTCACACTGGTGTGGCCCTGGCGGCGTTGATCAAGCTCCGGAATAAGGGGGTTATAGGCCCGACTGAGAGGACTGTGGTGGTGAGCACGGCTCATGGGTTGAAGTTTACGCAGGCGAAGATTGATTATCACTCTAAGGAGATTCCGGATTTGGCTTGCCGGTTTGCTAACCCACCGGTTCAAGTGAGGGCAGATTTTGGGTCGGTTATGGATGTTTTGAAGAAGTACTTGAGTAAGTCGGAGGCTCCAAAGCATTAG

Protein Analysis

518

Amino Acids

56.74

Weight (kDa)

6.54

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 154 - 462 2.9e-52 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 371, 455
AatII GACGTC 1 cut(s) 306
AccBSI CCGCTC 1 cut(s) 279
AccII CGCG 4 cut(s) 132, 144, 354, 372
AccIII TCCGGA 2 cut(s) 1335, 1441
AclWI GGATC 4 cut(s) 703, 716, 1166, 1179
AcoI YGGCCR 1 cut(s) 318
AcuI CTGAAG 1 cut(s) 1226
AcyI GRCGYC 5 cut(s) 5, 150, 303, 405, 645
AfaI GTAC 5 cut(s) 197, 275, 347, 1088, 1523
AfeI AGCGCT 1 cut(s) 500
AfiI CCNNNNNNNGG 9 cut(s) 265, 408, 490, 641, 705, 952, 1188, 1304, 1342
AflIII ACRYGT 1 cut(s) 225
AgeI ACCGGT 1 cut(s) 1471
AgsI TTSAA 6 cut(s) 208, 574, 1123, 1399, 1478, 1516
AhdI GACNNNNNGTC 1 cut(s) 229
AjiI CACGTC 1 cut(s) 228
AjnI CCWGG 3 cut(s) 386, 603, 1314
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AleI CACNNNNGTG 2 cut(s) 1305, 1307
AloI GAACNNNNNNTCC 2 cut(s) 1103, 1135
AluBI AGCT 6 cut(s) 532, 757, 866, 908, 1067, 1333
AluI AGCT 6 cut(s) 532, 757, 866, 908, 1067, 1333
Alw21I GWGCWC 3 cut(s) 534, 1063, 1385
Alw26I GTCTC 2 cut(s) 463, 880
AlwI GGATC 4 cut(s) 703, 716, 1166, 1179
Aor13HI TCCGGA 2 cut(s) 1335, 1441
Aor51HI AGCGCT 1 cut(s) 500
AoxI GGCC 9 cut(s) 292, 318, 389, 492, 750, 895, 1191, 1311, 1354
ApeKI GCWGC 3 cut(s) 908, 925, 1067
ArsI GACNNNNNNTTYG 4 cut(s) 212, 244, 791, 823
AsiGI ACCGGT 1 cut(s) 1471
AspA2I CCTAGG 1 cut(s) 580
AspLEI GCGC 6 cut(s) 354, 501, 656, 698, 1204, 1267
AsuC2I CCSGG 3 cut(s) 706, 947, 966
AsuHPI GGTGA 2 cut(s) 79, 1390
AsuII TTCGAA 1 cut(s) 468
AvaII GGWCC 4 cut(s) 411, 427, 577, 760
AvrII CCTAGG 1 cut(s) 580
BamHI GGATCC 2 cut(s) 708, 1171
BanII GRGCYC 1 cut(s) 534
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
Bbv12I GWGCWC 3 cut(s) 534, 1063, 1385
BbvI GCAGC 3 cut(s) 895, 937, 1054
BccI CCATC 6 cut(s) 101, 742, 776, 806, 1211, 1246
BceAI ACGGC 5 cut(s) 355, 412, 520, 1178, 1401
BciT130I CCWGG 3 cut(s) 388, 605, 1316
BclI TGATCA 1 cut(s) 1326
BcnI CCSGG 3 cut(s) 706, 947, 966
BcoDI GTCTC 2 cut(s) 463, 880
BfaI CTAG 2 cut(s) 581, 1047
BfmI CTRYAG 2 cut(s) 843, 923
BfoI RGCGCY 1 cut(s) 502
BglI GCCNNNNNGGC 2 cut(s) 564, 1319
BglII AGATCT 1 cut(s) 741
BlnI CCTAGG 1 cut(s) 580
BlpI GCTNAGC 1 cut(s) 791
BmcAI AGTACT 2 cut(s) 347, 1523
Bme1390I CCNGG 6 cut(s) 388, 605, 706, 947, 966, 1316
Bme18I GGWCC 4 cut(s) 411, 427, 577, 760
BmeRI GACNNNNNGTC 1 cut(s) 229
BmgBI CACGTC 1 cut(s) 228
BmiI GGNNCC 6 cut(s) 400, 428, 568, 710, 1173, 1542
BmrFI CCNGG 6 cut(s) 388, 605, 706, 947, 966, 1316
BmrI ACTGGG 1 cut(s) 277
BmsI GCATC 4 cut(s) 561, 641, 804, 1246
BmuI ACTGGG 1 cut(s) 277
BplI GAGNNNNNCTC 6 cut(s) 239, 271, 960, 992, 1372, 1404
BpmI CTGGAG 1 cut(s) 911
Bpu1102I GCTNAGC 1 cut(s) 791
Bpu14I TTCGAA 1 cut(s) 468
BpuEI CTTGAG 3 cut(s) 311, 1047, 1546
BpuMI CCSGG 3 cut(s) 706, 947, 966
BsaHI GRCGYC 5 cut(s) 5, 150, 303, 405, 645
BsaJI CCNNGG 4 cut(s) 517, 580, 1239, 1314
BsaWI WCCGGW 3 cut(s) 1335, 1441, 1471
BsaXI ACNNNNNCTCC 8 cut(s) 170, 200, 443, 473, 551, 581, 683, 713
Bsc4I CCNNNNNNNGG 9 cut(s) 265, 408, 490, 641, 705, 952, 1188, 1304, 1342
Bse118I RCCGGY 5 cut(s) 563, 662, 730, 1455, 1471
Bse1I ACTGG 5 cut(s) 283, 353, 894, 1285, 1309
Bse3DI GCAATG 1 cut(s) 1266
BseAI TCCGGA 2 cut(s) 1335, 1441
BseBI CCWGG 3 cut(s) 388, 605, 1316
BseDI CCNNGG 4 cut(s) 517, 580, 1239, 1314
BseGI GGATG 6 cut(s) 138, 656, 1117, 1222, 1261, 1513
BseLI CCNNNNNNNGG 9 cut(s) 265, 408, 490, 641, 705, 952, 1188, 1304, 1342
BseMI GCAATG 1 cut(s) 1266
BseMII CTCAG 3 cut(s) 426, 782, 1353
BseNI ACTGG 5 cut(s) 283, 353, 894, 1285, 1309
BseXI GCAGC 3 cut(s) 895, 937, 1054
BseYI CCCAGC 1 cut(s) 753
Bsh1236I CGCG 4 cut(s) 132, 144, 354, 372
Bsh1285I CGRYCG 1 cut(s) 1239
BshFI GGCC 9 cut(s) 294, 320, 391, 494, 752, 897, 1193, 1313, 1356
BshTI ACCGGT 1 cut(s) 1471
BsiEI CGRYCG 1 cut(s) 1239
BsiHKAI GWGCWC 3 cut(s) 534, 1063, 1385
BslFI GGGAC 2 cut(s) 185, 929
BslI CCNNNNNNNGG 9 cut(s) 265, 408, 490, 641, 705, 952, 1188, 1304, 1342
BsmAI GTCTC 2 cut(s) 463, 880
BsmBI CGTCTC 1 cut(s) 463
BsmFI GGGAC 2 cut(s) 185, 929
BsmI GAATGC 1 cut(s) 992
BsnI GGCC 9 cut(s) 294, 320, 391, 494, 752, 897, 1193, 1313, 1356
Bsp119I TTCGAA 1 cut(s) 468
Bsp1286I GDGCHC 3 cut(s) 534, 1063, 1385
Bsp13I TCCGGA 2 cut(s) 1335, 1441
Bsp143I GATC 5 cut(s) 438, 708, 741, 1171, 1326
Bsp1720I GCTNAGC 1 cut(s) 791
BspANI GGCC 9 cut(s) 294, 320, 391, 494, 752, 897, 1193, 1313, 1356
BspCNI CTCAG 3 cut(s) 427, 783, 1354
BspEI TCCGGA 2 cut(s) 1335, 1441
BspFNI CGCG 4 cut(s) 132, 144, 354, 372
BspLI GGNNCC 6 cut(s) 400, 428, 568, 710, 1173, 1542
BspMAI CTGCAG 2 cut(s) 847, 927
BspPI GGATC 4 cut(s) 703, 716, 1166, 1179
BspT104I TTCGAA 1 cut(s) 468
BsrBI CCGCTC 1 cut(s) 279
BsrDI GCAATG 1 cut(s) 1266
BsrFI RCCGGY 5 cut(s) 563, 662, 730, 1455, 1471
BsrI ACTGG 5 cut(s) 283, 353, 894, 1285, 1309
BssAI RCCGGY 5 cut(s) 563, 662, 730, 1455, 1471
BssECI CCNNGG 4 cut(s) 517, 580, 1239, 1314
BssMI GATC 5 cut(s) 438, 708, 741, 1171, 1326
BssNI GRCGYC 5 cut(s) 5, 150, 303, 405, 645
BssT1I CCWWGG 1 cut(s) 580
Bst2UI CCWGG 3 cut(s) 388, 605, 1316
Bst4CI ACNGT 4 cut(s) 514, 592, 881, 1372
Bst6I CTCTTC 3 cut(s) 23, 365, 491
BstACI GRCGYC 5 cut(s) 5, 150, 303, 405, 645
BstBI TTCGAA 1 cut(s) 468
BstC8I GCNNGC 4 cut(s) 732, 1269, 1410, 1453
BstDEI CTNAG 4 cut(s) 435, 791, 1362, 1431
BstENI CCTNNNNNAGG 1 cut(s) 263
BstF5I GGATG 6 cut(s) 138, 656, 1117, 1222, 1261, 1513
BstFNI CGCG 4 cut(s) 132, 144, 354, 372
BstH2I RGCGCY 1 cut(s) 502
BstHHI GCGC 6 cut(s) 354, 501, 656, 698, 1204, 1267
BstKTI GATC 5 cut(s) 441, 711, 744, 1174, 1329
BstMAI GTCTC 2 cut(s) 463, 880
BstMBI GATC 5 cut(s) 438, 708, 741, 1171, 1326
BstMCI CGRYCG 1 cut(s) 1239
BstNI CCWGG 3 cut(s) 388, 605, 1316
BstNSI RCATGY 1 cut(s) 1288
BstSCI CCNGG 6 cut(s) 386, 603, 704, 945, 964, 1314
BstSFI CTRYAG 2 cut(s) 843, 923
BstUI CGCG 4 cut(s) 132, 144, 354, 372
BstV1I GCAGC 3 cut(s) 895, 937, 1054
BstX2I RGATCY 3 cut(s) 708, 741, 1171
BstYI RGATCY 3 cut(s) 708, 741, 1171
BsuRI GGCC 9 cut(s) 294, 320, 391, 494, 752, 897, 1193, 1313, 1356
BtgZI GCGATG 1 cut(s) 754
BtrI CACGTC 1 cut(s) 228
BtsCI GGATG 6 cut(s) 138, 656, 1117, 1222, 1261, 1513
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 2 cut(s) 544, 1302
Cac8I GCNNGC 4 cut(s) 732, 1269, 1410, 1453
CfoI GCGC 6 cut(s) 354, 501, 656, 698, 1204, 1267
Cfr10I RCCGGY 5 cut(s) 563, 662, 730, 1455, 1471
CseI GACGC 5 cut(s) 158, 361, 394, 634, 1140
Csp6I GTAC 5 cut(s) 196, 274, 346, 1087, 1522
CspAI ACCGGT 1 cut(s) 1471
CviAII CATG 5 cut(s) 316, 523, 586, 1285, 1391
CviQI GTAC 5 cut(s) 196, 274, 346, 1087, 1522
DdeI CTNAG 4 cut(s) 435, 791, 1362, 1431
DpnI GATC 5 cut(s) 440, 710, 743, 1173, 1328
DpnII GATC 5 cut(s) 438, 708, 741, 1171, 1326
DrdI GACNNNNNNGTC 2 cut(s) 371, 455
DriI GACNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 2 cut(s) 371, 455
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 3 cut(s) 23, 365, 491
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 3 cut(s) 23, 365, 491
EciI GGCGGA 7 cut(s) 61, 106, 178, 451, 664, 676, 691
Ecl136II GAGCTC 1 cut(s) 532
Eco130I CCWWGG 1 cut(s) 580
Eco24I GRGCYC 1 cut(s) 534
Eco47I GGWCC 4 cut(s) 411, 427, 577, 760
Eco47III AGCGCT 1 cut(s) 500
Eco53kI GAGCTC 1 cut(s) 532
Eco57I CTGAAG 1 cut(s) 1226
EcoICRI GAGCTC 1 cut(s) 532
EcoNI CCTNNNNNAGG 1 cut(s) 263
EcoO109I RGGNCCY 2 cut(s) 427, 577
EcoRII CCWGG 3 cut(s) 386, 603, 1314
EcoT14I CCWWGG 1 cut(s) 580
EcoT22I ATGCAT 1 cut(s) 994
EcoT38I GRGCYC 1 cut(s) 534
ErhI CCWWGG 1 cut(s) 580
Esp3I CGTCTC 1 cut(s) 463
FaeI CATG 5 cut(s) 319, 526, 589, 1288, 1394
FalI AAGNNNNNCTT 2 cut(s) 1508, 1540
FaqI GGGAC 2 cut(s) 185, 929
FatI CATG 5 cut(s) 315, 522, 585, 1284, 1390
FauI CCCGC 1 cut(s) 377
FauNDI CATATG 1 cut(s) 1011
FbaI TGATCA 1 cut(s) 1326
FokI GGATG 6 cut(s) 125, 663, 1124, 1229, 1268, 1520
FriOI GRGCYC 1 cut(s) 534
FspBI CTAG 2 cut(s) 581, 1047
GlaI GCGC 6 cut(s) 353, 500, 655, 697, 1203, 1266
GsaI CCCAGC 1 cut(s) 757
GsuI CTGGAG 1 cut(s) 911
HaeII RGCGCY 1 cut(s) 502
HaeIII GGCC 9 cut(s) 294, 320, 391, 494, 752, 897, 1193, 1313, 1356
HgaI GACGC 5 cut(s) 158, 361, 394, 634, 1140
HhaI GCGC 6 cut(s) 354, 501, 656, 698, 1204, 1267
Hin1I GRCGYC 5 cut(s) 5, 150, 303, 405, 645
Hin1II CATG 5 cut(s) 319, 526, 589, 1288, 1394
Hin6I GCGC 6 cut(s) 352, 499, 654, 696, 1202, 1265
HinP1I GCGC 6 cut(s) 352, 499, 654, 696, 1202, 1265
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HphI GGTGA 2 cut(s) 79, 1390
Hpy166II GTNNAC 3 cut(s) 274, 1036, 1404
Hpy188I TCNGA 9 cut(s) 235, 241, 247, 650, 828, 980, 1164, 1216, 1537
Hpy188III TCNNGA 5 cut(s) 328, 745, 833, 1336, 1442
Hpy8I GTNNAC 3 cut(s) 274, 1036, 1404
HpyAV CCTTC 6 cut(s) 43, 214, 464, 475, 580, 1126
HpyCH4III ACNGT 4 cut(s) 514, 592, 881, 1372
HpyCH4IV ACGT 3 cut(s) 198, 227, 303
HpyCH4V TGCA 8 cut(s) 845, 925, 992, 1070, 1076, 1093, 1157, 1259
HpyF3I CTNAG 4 cut(s) 435, 791, 1362, 1431
HpySE526I ACGT 3 cut(s) 198, 227, 303
Hsp92I GRCGYC 5 cut(s) 5, 150, 303, 405, 645
Hsp92II CATG 5 cut(s) 319, 526, 589, 1288, 1394
HspAI GCGC 6 cut(s) 352, 499, 654, 696, 1202, 1265
Kpn2I TCCGGA 2 cut(s) 1335, 1441
KroI GCCGGC 1 cut(s) 730
KroNI GCCGGC 1 cut(s) 732
Ksp22I TGATCA 1 cut(s) 1326
Kzo9I GATC 5 cut(s) 438, 708, 741, 1171, 1326
LmnI GCTCC 6 cut(s) 107, 284, 404, 572, 1338, 1546
Lsp1109I GCAGC 3 cut(s) 895, 937, 1054
LweI GCATC 4 cut(s) 561, 641, 804, 1246
MaeI CTAG 2 cut(s) 581, 1047
MaeII ACGT 3 cut(s) 198, 227, 303
MaeIII GTNAC 1 cut(s) 838
MalI GATC 5 cut(s) 440, 710, 743, 1173, 1328
MbiI CCGCTC 1 cut(s) 279
MboI GATC 5 cut(s) 438, 708, 741, 1171, 1326
MboII GAAGA 6 cut(s) 10, 352, 478, 1219, 1426, 1528
MflI RGATCY 3 cut(s) 708, 741, 1171
MhlI GDGCHC 3 cut(s) 534, 1063, 1385
MluCI AATT 4 cut(s) 848, 868, 1022, 1158
MlyI GAGTC 4 cut(s) 245, 251, 257, 359
MmeI TCCRAC 4 cut(s) 504, 628, 1194, 1515
Mph1103I ATGCAT 1 cut(s) 994
MroI TCCGGA 2 cut(s) 1335, 1441
MroNI GCCGGC 1 cut(s) 730
MslI CAYNNNNRTG 2 cut(s) 1305, 1307
MspA1I CMGCKG 2 cut(s) 757, 908
MspR9I CCNGG 6 cut(s) 388, 605, 706, 947, 966, 1316
Mva1269I GAATGC 1 cut(s) 992
MvaI CCWGG 3 cut(s) 388, 605, 1316
MvnI CGCG 4 cut(s) 132, 144, 354, 372
NaeI GCCGGC 1 cut(s) 732
NciI CCSGG 3 cut(s) 706, 947, 966
NdeI CATATG 1 cut(s) 1011
NdeII GATC 5 cut(s) 438, 708, 741, 1171, 1326
NgoMIV GCCGGC 1 cut(s) 730
NlaIII CATG 5 cut(s) 319, 526, 589, 1288, 1394
NlaIV GGNNCC 6 cut(s) 400, 428, 568, 710, 1173, 1542
NmeAIII GCCGAG 2 cut(s) 498, 520
NmuCI GTSAC 1 cut(s) 838
NsiI ATGCAT 1 cut(s) 994
NspI RCATGY 1 cut(s) 1288
NspV TTCGAA 1 cut(s) 468
OliI CACNNNNGTG 2 cut(s) 1305, 1307
PcsI WCGNNNNNNNCGW 6 cut(s) 267, 309, 369, 402, 447, 525
PctI GAATGC 1 cut(s) 992
PdiI GCCGGC 1 cut(s) 732
PfeI GAWTC 6 cut(s) 823, 829, 919, 1042, 1274, 1438
PflFI GACNNNGTC 2 cut(s) 266, 455
PfoI TCCNGGA 2 cut(s) 945, 964
PinAI ACCGGT 1 cut(s) 1471
PleI GAGTC 4 cut(s) 244, 250, 256, 359
PpsI GAGTC 4 cut(s) 244, 250, 256, 359
PpuMI RGGWCCY 2 cut(s) 427, 577
Psp124BI GAGCTC 1 cut(s) 534
Psp5II RGGWCCY 2 cut(s) 427, 577
Psp6I CCWGG 3 cut(s) 386, 603, 1314
PspFI CCCAGC 1 cut(s) 753
PspGI CCWGG 3 cut(s) 386, 603, 1314
PspN4I GGNNCC 6 cut(s) 400, 428, 568, 710, 1173, 1542
PspPPI RGGWCCY 2 cut(s) 427, 577
PstI CTGCAG 2 cut(s) 847, 927
PsuI RGATCY 3 cut(s) 708, 741, 1171
PsyI GACNNNGTC 2 cut(s) 266, 455
PvuII CAGCTG 2 cut(s) 757, 908
RsaI GTAC 5 cut(s) 197, 275, 347, 1088, 1523
RsaNI GTAC 5 cut(s) 196, 274, 346, 1087, 1522
RseI CAYNNNNRTG 2 cut(s) 1305, 1307
SacI GAGCTC 1 cut(s) 534
Sau3AI GATC 5 cut(s) 438, 708, 741, 1171, 1326
ScaI AGTACT 2 cut(s) 347, 1523
SchI GAGTC 4 cut(s) 245, 251, 257, 359
ScrFI CCNGG 6 cut(s) 388, 605, 706, 947, 966, 1316
SduI GDGCHC 3 cut(s) 534, 1063, 1385
SfaNI GCATC 4 cut(s) 561, 641, 804, 1246
SfcI CTRYAG 2 cut(s) 843, 923
SfuI TTCGAA 1 cut(s) 468
SgrAI CRCCGGYG 1 cut(s) 662
SinI GGWCC 4 cut(s) 411, 427, 577, 760
SmiMI CAYNNNNRTG 2 cut(s) 1305, 1307
SmlI CTYRAG 3 cut(s) 326, 1062, 1525
SmoI CTYRAG 3 cut(s) 326, 1062, 1525
Sse9I AATT 4 cut(s) 848, 868, 1022, 1158
SspMI CTAG 2 cut(s) 581, 1047
SstI GAGCTC 1 cut(s) 534
StyD4I CCNGG 6 cut(s) 386, 603, 704, 945, 964, 1314
StyI CCWWGG 1 cut(s) 580
TaaI ACNGT 4 cut(s) 514, 592, 881, 1372
TaiI ACGT 3 cut(s) 201, 230, 306
TaqI TCGA 9 cut(s) 261, 300, 363, 441, 468, 746, 798, 810, 832
TaqII GACCGA 1 cut(s) 1253
TasI AATT 4 cut(s) 848, 868, 1022, 1158
TatI WGTACW 3 cut(s) 345, 1086, 1521
TauI GCSGC 7 cut(s) 122, 132, 294, 323, 552, 680, 1322
TfiI GAWTC 6 cut(s) 823, 829, 919, 1042, 1274, 1438
TscAI CASTG 2 cut(s) 551, 1309
TseFI GTSAC 1 cut(s) 838
TseI GCWGC 3 cut(s) 908, 925, 1067
Tsp45I GTSAC 1 cut(s) 838
TspDTI ATGAA 4 cut(s) 539, 641, 836, 998
TspRI CASTG 2 cut(s) 551, 1309
Tth111I GACNNNGTC 2 cut(s) 266, 455
VpaK11BI GGWCC 4 cut(s) 411, 427, 577, 760
XagI CCTNNNNNAGG 1 cut(s) 263
XceI RCATGY 1 cut(s) 1288
XmaJI CCTAGG 1 cut(s) 580
XspI CTAG 2 cut(s) 581, 1047
ZraI GACGTC 1 cut(s) 304
ZrmI AGTACT 2 cut(s) 347, 1523
Zsp2I ATGCAT 1 cut(s) 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.