MD17G1171300.v1.1

Threonine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005631
Physical Location & Seq
Forward (+)
17644007 .. 17646012
2006 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1171300.v1.1.491

Sequence Viewer

Length: 1569 bp
ATGGCCTCTTCCTCTCTCTTCCAATCTTCCCCTCTCTCCCTCAAACCACAAAATCCCACCTCCCTCCATCCCTCAACCAAACCCCATTTCGCCACCACCATCAAATGCGTCTCCACCTCCACTCCTCCCTCCTCCTCTTCTTCCTCCTCCGACCACCCTCCCCACAACATTCGCGACGAGGCTCGCCGCCACAACACCACCCACTCCCACCACTTCTCCGCCCGCTACGTCCCCTTCAACGCCGACCCCTCCTCCTCCGCCGCCGAGTCCTACTCCCTCGACGAGATCGTTTACCGATCCAACTCCGGCGGCCTCCTCGACGTCCAGCACGACATGGCCGCCCTCAAAAACTACGACGGCAAGTACTGGCGCGACCTCTTCGACTCCCGGATCGGCAAGACCACCTGGCCCTACGGCTCCGGCGTTTGGAGCAAGAAGGAGTGGGTCCTACCCGAGATCGACAGCGACGACATTGTCTCCGCTTTTGAAGGTAACTCCAATCTCTTCTGGGCTGAGCGTTATGGCAAACAGTTTCTAAGCATGAACGATTTGTGGGTCAAACACTGTGGGATTAGCCACACTGGCAGTTTTAAGGACTTGGGCATGACTGTTTTGGTCAGCCAAGTGAACCGGCTGAGGAAAATGAACCGCCCCGTGGTCGGCGTCGGCTGTGCCTCCACCGGAGACACATCCGCCGCCTTATCGGCCTACTGTGCCGCAGCTGGGATACCATCGATTGTGTTTCTACCCGCAAATCGGATATCTCTGGCTCAGCTGGTTCAACCCATTGCCAATGGGGCCTTCGTGCTGAGCATTGATACTGATTTCGATGGTTGTATGCAGCTGATTCGAGAAGTCACGGCGGAGCTACCGATTTATTTGGCCAATTCTTTGAACAGTTTGAGATTAGAGGGTCAAAAAACCGCCGCAATAGAGATTTTGCAGCAGTTTGATTGGGAAGTGCCGGATTGGGTCATAGTTCCAGGGGGAAATTTGGGTAACATATATGCTTTCTACAAAGGGTTTCATATGTGCAAAGAGTTGGGGCTAGTAGATAAGATTCCTAGGCTTGTTTGTGCCCAAGCTGCAAATGCGAACCCGCTGTACTTGCATTACAAGGCAGGGTGGGATCAAGAGTTCAAGCCCGTGAAGGCGAACACTACGTTTGCATCCGCAATCCAAATAGGTGATCCTGTTTCGATAGACAGAGCCGTGTATGCACTGAAGAAGTCGAATGGGATTGTGGAGGAAGCCAGTGAGCAGGAGTTGATGGATGCCATGGCTCAAGCAGACTCCACTGGCATGTTCATATGTCCTCACACTGGTGTGGCATTGGCTGCATTGATTAAGCTTCGGAACCAAGGGATCATAGGCACAAATGACCGGACTGTGGTGGTGAGCACGGCTCATGGGTTGAAATTTACGCAGTCGAAGATTGATTATCATTCAAATGATATCAAGGATTTGGCTTGCAAGTATGCTAACCCGCCAATGCAAGTGAAGGCGGATTTTGGGTCTGTGATGGATGTTTTGAAGAAGTACTTGTTGAGTAAGGTGCCAAAGAATTAG

Protein Analysis

523

Amino Acids

57.22

Weight (kDa)

6.96

Isoelectric Point (pI)

31.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 160 - 468 4.7e-53 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 473
AatII GACGTC 1 cut(s) 324
AccB1I GGYRCC 1 cut(s) 1555
AccII CGCG 2 cut(s) 174, 372
AclWI GGATC 5 cut(s) 291, 398, 1137, 1184, 1373
AcoI YGGCCR 2 cut(s) 336, 882
AcsI RAATTY 2 cut(s) 991, 1418
AcuI CTGAAG 1 cut(s) 1244
AcyI GRCGYC 2 cut(s) 321, 663
AfaI GTAC 3 cut(s) 365, 1106, 1541
AfiI CCNNNNNNNGG 7 cut(s) 426, 655, 659, 723, 756, 1322, 1390
AgsI TTSAA 8 cut(s) 238, 488, 782, 895, 1141, 1417, 1449, 1534
AjnI CCWGG 2 cut(s) 404, 982
AjuI GAANNNNNNNTTGG 4 cut(s) 71, 103, 785, 817
AleI CACNNNNGTG 2 cut(s) 1323, 1325
AloI GAACNNNNNNTCC 2 cut(s) 1121, 1153
AluBI AGCT 6 cut(s) 722, 775, 844, 868, 1085, 1351
AluI AGCT 6 cut(s) 722, 775, 844, 868, 1085, 1351
Alw21I GWGCWC 1 cut(s) 1403
Alw26I GTCTC 3 cut(s) 115, 481, 678
AlwI GGATC 5 cut(s) 291, 398, 1137, 1184, 1373
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 7 cut(s) 3, 310, 336, 407, 705, 798, 882
ApeKI GCWGC 5 cut(s) 719, 841, 943, 1085, 1337
ApoI RAATTY 2 cut(s) 991, 1418
AspA2I CCTAGG 1 cut(s) 1064
AspLEI GCGC 1 cut(s) 372
AspS9I GGNCC 3 cut(s) 408, 445, 798
AsuC2I CCSGG 1 cut(s) 388
AsuHPI GGTGA 2 cut(s) 1199, 1408
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 1 cut(s) 445
AvrII CCTAGG 1 cut(s) 1064
BaeGI GKGCMC 1 cut(s) 1081
BalI TGGCCA 1 cut(s) 884
BanI GGYRCC 1 cut(s) 1555
Bbv12I GWGCWC 1 cut(s) 1403
BbvCI CCTCAGC 1 cut(s) 635
BbvI GCAGC 5 cut(s) 731, 853, 955, 1072, 1324
BccI CCATC 6 cut(s) 75, 107, 739, 824, 1264, 1516
BceAI ACGGC 5 cut(s) 373, 430, 876, 1196, 1419
BcgI CGANNNNNNTGC 2 cut(s) 830, 864
BciT130I CCWGG 2 cut(s) 406, 984
BciVI GTATCC 1 cut(s) 720
BcnI CCSGG 1 cut(s) 388
BcoDI GTCTC 3 cut(s) 115, 481, 678
BfaI CTAG 2 cut(s) 1049, 1065
BfuI GTATCC 1 cut(s) 720
BglI GCCNNNNNGGC 3 cut(s) 582, 704, 797
BlnI CCTAGG 1 cut(s) 1064
BlpI GCTNAGC 3 cut(s) 513, 771, 809
BmcAI AGTACT 2 cut(s) 365, 1541
Bme1390I CCNGG 3 cut(s) 388, 406, 984
Bme18I GGWCC 1 cut(s) 445
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 3 cut(s) 408, 445, 798
BmiI GGNNCC 5 cut(s) 418, 446, 799, 1358, 1557
BmrFI CCNGG 3 cut(s) 388, 406, 984
BmsI GCATC 2 cut(s) 1178, 1264
BplI GAGNNNNNCTC 4 cut(s) 257, 289, 1390, 1422
Bpu10I CCTNAGC 1 cut(s) 635
Bpu1102I GCTNAGC 3 cut(s) 513, 771, 809
BpuEI CTTGAG 1 cut(s) 1269
BpuMI CCSGG 1 cut(s) 388
Bsa29I ATCGAT 1 cut(s) 734
BsaHI GRCGYC 2 cut(s) 321, 663
BsaJI CCNNGG 5 cut(s) 654, 983, 1064, 1278, 1360
BsaWI WCCGGW 2 cut(s) 680, 1383
BsaXI ACNNNNNCTCC 8 cut(s) 106, 136, 200, 230, 236, 266, 461, 491
Bsc4I CCNNNNNNNGG 7 cut(s) 426, 655, 659, 723, 756, 1322, 1390
Bse118I RCCGGY 1 cut(s) 630
Bse1I ACTGG 5 cut(s) 371, 586, 1254, 1303, 1327
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 2 cut(s) 406, 984
BseCI ATCGAT 1 cut(s) 734
BseDI CCNNGG 5 cut(s) 654, 983, 1064, 1278, 1360
BseGI GGATG 5 cut(s) 67, 689, 1169, 1279, 1531
BseLI CCNNNNNNNGG 7 cut(s) 426, 655, 659, 723, 756, 1322, 1390
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 4 cut(s) 504, 626, 785, 800
BseNI ACTGG 5 cut(s) 371, 586, 1254, 1303, 1327
BseRI GAGGAG 7 cut(s) 114, 121, 124, 136, 241, 244, 305
BseSI GKGCMC 1 cut(s) 1081
BseXI GCAGC 5 cut(s) 731, 853, 955, 1072, 1324
BseYI CCCAGC 1 cut(s) 722
Bsh1236I CGCG 2 cut(s) 174, 372
BshFI GGCC 7 cut(s) 5, 312, 338, 409, 707, 800, 884
BshNI GGYRCC 1 cut(s) 1555
BshVI ATCGAT 1 cut(s) 734
BsiHKAI GWGCWC 1 cut(s) 1403
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 7 cut(s) 306, 388, 420, 631, 681, 965, 1384
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 7 cut(s) 426, 655, 659, 723, 756, 1322, 1390
BsmAI GTCTC 3 cut(s) 115, 481, 678
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 7 cut(s) 5, 312, 338, 409, 707, 800, 884
BsoBI CYCGRG 1 cut(s) 452
Bsp1286I GDGCHC 2 cut(s) 1081, 1403
Bsp143I GATC 7 cut(s) 285, 296, 390, 456, 1129, 1189, 1365
Bsp1720I GCTNAGC 3 cut(s) 513, 771, 809
Bsp19I CCATGG 1 cut(s) 1278
Bsp68I TCGCGA 1 cut(s) 174
BspANI GGCC 7 cut(s) 5, 312, 338, 409, 707, 800, 884
BspCNI CTCAG 4 cut(s) 505, 627, 784, 801
BspDI ATCGAT 1 cut(s) 734
BspFNI CGCG 2 cut(s) 174, 372
BspLI GGNNCC 5 cut(s) 418, 446, 799, 1358, 1557
BspPI GGATC 5 cut(s) 291, 398, 1137, 1184, 1373
BspT107I GGYRCC 1 cut(s) 1555
BsrDI GCAATG 1 cut(s) 786
BsrFI RCCGGY 1 cut(s) 630
BsrI ACTGG 5 cut(s) 371, 586, 1254, 1303, 1327
BssAI RCCGGY 1 cut(s) 630
BssECI CCNNGG 5 cut(s) 654, 983, 1064, 1278, 1360
BssMI GATC 7 cut(s) 285, 296, 390, 456, 1129, 1189, 1365
BssNI GRCGYC 2 cut(s) 321, 663
BssT1I CCWWGG 3 cut(s) 1064, 1278, 1360
Bst2UI CCWGG 2 cut(s) 406, 984
Bst4CI ACNGT 6 cut(s) 531, 566, 610, 713, 899, 1390
Bst6I CTCTTC 5 cut(s) 13, 23, 142, 383, 509
BstACI GRCGYC 2 cut(s) 321, 663
BstAPI GCANNNNNTGC 1 cut(s) 1337
BstC8I GCNNGC 3 cut(s) 184, 223, 1471
BstDEI CTNAG 5 cut(s) 513, 536, 635, 771, 809
BstDSI CCRYGG 2 cut(s) 654, 1278
BstF5I GGATG 5 cut(s) 67, 689, 1169, 1279, 1531
BstFNI CGCG 2 cut(s) 174, 372
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 7 cut(s) 288, 299, 393, 459, 1132, 1192, 1368
BstMAI GTCTC 3 cut(s) 115, 481, 678
BstMBI GATC 7 cut(s) 285, 296, 390, 456, 1129, 1189, 1365
BstNI CCWGG 2 cut(s) 406, 984
BstNSI RCATGY 1 cut(s) 1306
BstSCI CCNGG 3 cut(s) 386, 404, 982
BstSLI GKGCMC 1 cut(s) 1081
BstUI CGCG 2 cut(s) 174, 372
BstV1I GCAGC 5 cut(s) 731, 853, 955, 1072, 1324
Bsu15I ATCGAT 1 cut(s) 734
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 7 cut(s) 5, 312, 338, 409, 707, 800, 884
BsuTUI ATCGAT 1 cut(s) 734
BtgI CCRYGG 2 cut(s) 654, 1278
BtsCI GGATG 5 cut(s) 67, 689, 1169, 1279, 1531
BtsIMutI CAGTG 6 cut(s) 562, 579, 1220, 1261, 1296, 1320
BtuMI TCGCGA 1 cut(s) 174
Cac8I GCNNGC 3 cut(s) 184, 223, 1471
CfoI GCGC 1 cut(s) 372
Cfr10I RCCGGY 1 cut(s) 630
Cfr13I GGNCC 3 cut(s) 408, 445, 798
ClaI ATCGAT 1 cut(s) 734
CseI GACGC 2 cut(s) 97, 652
Csp6I GTAC 3 cut(s) 364, 1105, 1540
CspCI CAANNNNNGTGG 2 cut(s) 547, 582
CviAII CATG 6 cut(s) 334, 541, 604, 1279, 1303, 1409
CviQI GTAC 3 cut(s) 364, 1105, 1540
DdeI CTNAG 5 cut(s) 513, 536, 635, 771, 809
DpnI GATC 7 cut(s) 287, 298, 392, 458, 1131, 1191, 1367
DpnII GATC 7 cut(s) 285, 296, 390, 456, 1129, 1189, 1365
DrdI GACNNNNNNGTC 1 cut(s) 473
DseDI GACNNNNNNGTC 1 cut(s) 473
EaeI YGGCCR 2 cut(s) 336, 882
Eam1104I CTCTTC 5 cut(s) 13, 23, 142, 383, 509
EarI CTCTTC 5 cut(s) 13, 23, 142, 383, 509
EciI GGCGGA 5 cut(s) 208, 247, 682, 878, 1520
Eco130I CCWWGG 3 cut(s) 1064, 1278, 1360
Eco32I GATATC 2 cut(s) 762, 1456
Eco47I GGWCC 1 cut(s) 445
Eco57I CTGAAG 1 cut(s) 1244
Eco88I CYCGRG 1 cut(s) 452
EcoO109I RGGNCCY 2 cut(s) 445, 798
EcoRII CCWGG 2 cut(s) 404, 982
EcoRV GATATC 2 cut(s) 762, 1456
EcoT14I CCWWGG 3 cut(s) 1064, 1278, 1360
ErhI CCWWGG 3 cut(s) 1064, 1278, 1360
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 6 cut(s) 337, 544, 607, 1282, 1306, 1412
FalI AAGNNNNNCTT 2 cut(s) 1526, 1558
FaqI GGGAC 1 cut(s) 215
FatI CATG 6 cut(s) 333, 540, 603, 1278, 1302, 1408
FauI CCCGC 4 cut(s) 230, 757, 1107, 1494
FauNDI CATATG 2 cut(s) 1029, 1310
FokI GGATG 5 cut(s) 54, 676, 1156, 1286, 1538
FspBI CTAG 2 cut(s) 1049, 1065
GlaI GCGC 1 cut(s) 371
GsaI CCCAGC 1 cut(s) 726
HaeIII GGCC 7 cut(s) 5, 312, 338, 409, 707, 800, 884
HapII CCGG 7 cut(s) 306, 388, 420, 631, 681, 965, 1384
HgaI GACGC 2 cut(s) 97, 652
HhaI GCGC 1 cut(s) 372
Hin1I GRCGYC 2 cut(s) 321, 663
Hin1II CATG 6 cut(s) 337, 544, 607, 1282, 1306, 1412
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 1349
HinfI GANTC 5 cut(s) 266, 383, 847, 1060, 1292
HpaII CCGG 7 cut(s) 306, 388, 420, 631, 681, 965, 1384
HphI GGTGA 2 cut(s) 1199, 1408
Hpy166II GTNNAC 2 cut(s) 292, 628
Hpy188I TCNGA 3 cut(s) 151, 759, 1356
Hpy188III TCNNGA 3 cut(s) 173, 851, 1133
Hpy8I GTNNAC 2 cut(s) 292, 628
Hpy99I CGWCG 6 cut(s) 179, 284, 323, 359, 470, 668
HpyAV CCTTC 6 cut(s) 244, 430, 482, 811, 1144, 1495
HpyCH4III ACNGT 6 cut(s) 531, 566, 610, 713, 899, 1390
HpyCH4IV ACGT 3 cut(s) 228, 321, 1163
HpyF3I CTNAG 5 cut(s) 513, 536, 635, 771, 809
HpySE526I ACGT 3 cut(s) 228, 321, 1163
Hsp92I GRCGYC 2 cut(s) 321, 663
Hsp92II CATG 6 cut(s) 337, 544, 607, 1282, 1306, 1412
HspAI GCGC 1 cut(s) 370
Kzo9I GATC 7 cut(s) 285, 296, 390, 456, 1129, 1189, 1365
LmnI GCTCC 3 cut(s) 422, 429, 865
Lsp1109I GCAGC 5 cut(s) 731, 853, 955, 1072, 1324
LweI GCATC 2 cut(s) 1178, 1264
MaeI CTAG 2 cut(s) 1049, 1065
MaeII ACGT 3 cut(s) 228, 321, 1163
MaeIII GTNAC 3 cut(s) 491, 856, 998
MalI GATC 7 cut(s) 287, 298, 392, 458, 1131, 1191, 1367
MboI GATC 7 cut(s) 285, 296, 390, 456, 1129, 1189, 1365
MboII GAAGA 9 cut(s) 10, 18, 129, 132, 370, 496, 1237, 1444, 1546
MhlI GDGCHC 2 cut(s) 1081, 1403
MlsI TGGCCA 1 cut(s) 884
MluCI AATT 4 cut(s) 886, 991, 1418, 1564
MluNI TGGCCA 1 cut(s) 884
MlyI GAGTC 3 cut(s) 275, 377, 1286
MmeI TCCRAC 2 cut(s) 174, 324
Mox20I TGGCCA 1 cut(s) 884
MscI TGGCCA 1 cut(s) 884
MseI TTAA 2 cut(s) 591, 1347
MslI CAYNNNNRTG 4 cut(s) 1301, 1323, 1325, 1449
Msp20I TGGCCA 1 cut(s) 884
MspA1I CMGCKG 4 cut(s) 722, 775, 844, 1102
MspI CCGG 7 cut(s) 306, 388, 420, 631, 681, 965, 1384
MspR9I CCNGG 3 cut(s) 388, 406, 984
MvaI CCWGG 2 cut(s) 406, 984
MvnI CGCG 2 cut(s) 174, 372
NciI CCSGG 1 cut(s) 388
NcoI CCATGG 1 cut(s) 1278
NdeI CATATG 2 cut(s) 1029, 1310
NdeII GATC 7 cut(s) 285, 296, 390, 456, 1129, 1189, 1365
NlaIII CATG 6 cut(s) 337, 544, 607, 1282, 1306, 1412
NlaIV GGNNCC 5 cut(s) 418, 446, 799, 1358, 1557
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 856
NruI TCGCGA 1 cut(s) 174
NspI RCATGY 1 cut(s) 1306
OliI CACNNNNGTG 2 cut(s) 1323, 1325
PcsI WCGNNNNNNNCGW 4 cut(s) 285, 327, 420, 465
PfeI GAWTC 2 cut(s) 847, 1060
PflFI GACNNNGTC 1 cut(s) 473
PfoI TCCNGGA 1 cut(s) 386
PleI GAGTC 3 cut(s) 274, 377, 1286
PpsI GAGTC 3 cut(s) 274, 377, 1286
PpuMI RGGWCCY 1 cut(s) 445
Psp5II RGGWCCY 1 cut(s) 445
Psp6I CCWGG 2 cut(s) 404, 982
PspFI CCCAGC 1 cut(s) 722
PspGI CCWGG 2 cut(s) 404, 982
PspN4I GGNNCC 5 cut(s) 418, 446, 799, 1358, 1557
PspPI GGNCC 3 cut(s) 408, 445, 798
PspPPI RGGWCCY 1 cut(s) 445
PsrI GAACNNNNNNTAC 2 cut(s) 1088, 1120
PsyI GACNNNGTC 1 cut(s) 473
PvuII CAGCTG 3 cut(s) 722, 775, 844
RruI TCGCGA 1 cut(s) 174
RsaI GTAC 3 cut(s) 365, 1106, 1541
RsaNI GTAC 3 cut(s) 364, 1105, 1540
RseI CAYNNNNRTG 4 cut(s) 1301, 1323, 1325, 1449
SaqAI TTAA 2 cut(s) 591, 1347
Sau3AI GATC 7 cut(s) 285, 296, 390, 456, 1129, 1189, 1365
Sau96I GGNCC 3 cut(s) 408, 445, 798
ScaI AGTACT 2 cut(s) 365, 1541
SchI GAGTC 3 cut(s) 275, 377, 1286
ScrFI CCNGG 3 cut(s) 388, 406, 984
SduI GDGCHC 2 cut(s) 1081, 1403
SfaNI GCATC 2 cut(s) 1178, 1264
SinI GGWCC 1 cut(s) 445
SmiMI CAYNNNNRTG 4 cut(s) 1301, 1323, 1325, 1449
SmlI CTYRAG 1 cut(s) 1284
SmoI CTYRAG 1 cut(s) 1284
Sse9I AATT 4 cut(s) 886, 991, 1418, 1564
SspMI CTAG 2 cut(s) 1049, 1065
StyD4I CCNGG 3 cut(s) 386, 404, 982
StyI CCWWGG 3 cut(s) 1064, 1278, 1360
TaaI ACNGT 6 cut(s) 531, 566, 610, 713, 899, 1390
TaiI ACGT 3 cut(s) 231, 324, 1166
TasI AATT 4 cut(s) 886, 991, 1418, 1564
TatI WGTACW 3 cut(s) 363, 1104, 1539
TauI GCSGC 7 cut(s) 189, 263, 312, 341, 698, 719, 929
TfiI GAWTC 2 cut(s) 847, 1060
Tru1I TTAA 2 cut(s) 591, 1347
Tru9I TTAA 2 cut(s) 591, 1347
TscAI CASTG 6 cut(s) 569, 586, 1227, 1261, 1303, 1327
TseFI GTSAC 1 cut(s) 856
TseI GCWGC 5 cut(s) 719, 841, 943, 1085, 1337
Tsp45I GTSAC 1 cut(s) 856
TspDTI ATGAA 4 cut(s) 557, 659, 1016, 1297
TspRI CASTG 6 cut(s) 569, 586, 1227, 1261, 1303, 1327
Tth111I GACNNNGTC 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 445
XapI RAATTY 2 cut(s) 991, 1418
XceI RCATGY 1 cut(s) 1306
XmaJI CCTAGG 1 cut(s) 1064
XspI CTAG 2 cut(s) 1049, 1065
ZraI GACGTC 1 cut(s) 322
ZrmI AGTACT 2 cut(s) 365, 1541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.