pycom02g01320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005631
Physical Location & Seq
Forward (+)
771099 .. 771791
693 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g01320.2

Sequence Viewer

Length: 477 bp
ATGGCGACCAAAGAGGAGAGAAGCTTCATTGATAAGGTAATAATCAAGTGGTACGAAGGTCTAGAAATCCTCCAGATGGTGAAGAAGAGGGTTTCAACAGCGACAGCGATGATGGAGATCGATAGCTTGAAGGACGTCACTGCTGGTCAGAAGAAAGTCAAGTGGTTCGAGAAGTCAGAGATCCTCCAAATACTGAAGAAGATGTTTGATCCTTGGCTGAACGAAGATGGCATGCCTGACGCCACCTCTTTCTTCGGCCGTTGCCCTAGATGCTTTGGTAACATTTTTCTCTTCTTGTTAATATATACAACACCAACAACCGAGAGTCTTATCACAATAAGAGCCGTTCCGTTTTGCACCGTGTCTTCCGGTAGCTTCTTGTTAAGTGAAGTCCACACACTTCCCCTCTTTCTCCTCTCAGCCATGGTTTCCTCTGCCTTGCTCCACTCCTCGTACGGCTCCGGCGTCTGGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.86

Weight (kDa)

7.69

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AclWI GGATC 2 cut(s) 175, 203
AcoI YGGCCR 1 cut(s) 256
AcuI CTGAAG 1 cut(s) 215
AcyI GRCGYC 3 cut(s) 135, 240, 465
AfaI GTAC 2 cut(s) 53, 455
AfiI CCNNNNNNNGG 2 cut(s) 76, 468
AgsI TTSAA 2 cut(s) 96, 130
AluBI AGCT 3 cut(s) 24, 126, 375
AluI AGCT 3 cut(s) 24, 126, 375
AlwI GGATC 2 cut(s) 175, 203
AoxI GGCC 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 471
AsuHPI GGTGA 1 cut(s) 91
AvaII GGWCC 1 cut(s) 471
BaeI ACNNNNGTAYC 2 cut(s) 43, 76
BbsI GAAGAC 1 cut(s) 357
BccI CCATC 3 cut(s) 70, 106, 221
BceAI ACGGC 3 cut(s) 243, 329, 472
BfaI CTAG 3 cut(s) 62, 267, 475
Bme18I GGWCC 1 cut(s) 471
BmgT120I GGNCC 1 cut(s) 471
BmiI GGNNCC 1 cut(s) 460
BmsI GCATC 1 cut(s) 260
BpiI GAAGAC 1 cut(s) 357
BpmI CTGGAG 1 cut(s) 56
Bsa29I ATCGAT 1 cut(s) 120
BsaBI GATNNNNATC 1 cut(s) 116
BsaHI GRCGYC 3 cut(s) 135, 240, 465
BsaJI CCNNGG 2 cut(s) 212, 423
BsaWI WCCGGW 1 cut(s) 368
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 468
Bse8I GATNNNNATC 1 cut(s) 116
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 212, 423
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 2 cut(s) 76, 468
BseMII CTCAG 1 cut(s) 432
BseRI GAGGAG 3 cut(s) 29, 404, 439
BseX3I CGGCCG 1 cut(s) 256
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 1 cut(s) 258
BshVI ATCGAT 1 cut(s) 120
BsiEI CGRYCG 1 cut(s) 259
BsiSI CCGG 2 cut(s) 369, 462
BsiWI CGTACG 1 cut(s) 453
BslI CCNNNNNNNGG 2 cut(s) 76, 468
BsnI GGCC 1 cut(s) 258
Bsp143I GATC 3 cut(s) 117, 180, 208
Bsp19I CCATGG 1 cut(s) 423
BspANI GGCC 1 cut(s) 258
BspCNI CTCAG 1 cut(s) 431
BspDI ATCGAT 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 460
BspPI GGATC 2 cut(s) 175, 203
BssECI CCNNGG 2 cut(s) 212, 423
BssMI GATC 3 cut(s) 117, 180, 208
BssNI GRCGYC 3 cut(s) 135, 240, 465
BssT1I CCWWGG 2 cut(s) 212, 423
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 2 cut(s) 80, 296
BstACI GRCGYC 3 cut(s) 135, 240, 465
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 1 cut(s) 418
BstDSI CCRYGG 1 cut(s) 423
BstKTI GATC 3 cut(s) 120, 183, 211
BstMBI GATC 3 cut(s) 117, 180, 208
BstMCI CGRYCG 1 cut(s) 259
BstMWI GCNNNNNNNGC 1 cut(s) 270
BstNSI RCATGY 1 cut(s) 235
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BstZI CGGCCG 1 cut(s) 256
Bsu15I ATCGAT 1 cut(s) 120
BsuRI GGCC 1 cut(s) 258
BsuTUI ATCGAT 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 423
BtgZI GCGATG 1 cut(s) 122
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 471
ClaI ATCGAT 1 cut(s) 120
CseI GACGC 2 cut(s) 248, 454
Csp6I GTAC 2 cut(s) 52, 454
CviAII CATG 2 cut(s) 232, 424
CviJI RGCY 8 cut(s) 24, 126, 217, 258, 344, 375, 422, 459
CviKI_1 RGCY 8 cut(s) 24, 126, 217, 258, 344, 375, 422, 459
CviQI GTAC 2 cut(s) 52, 454
DdeI CTNAG 1 cut(s) 418
DpnI GATC 3 cut(s) 119, 182, 210
DpnII GATC 3 cut(s) 117, 180, 208
EaeI YGGCCR 1 cut(s) 256
EagI CGGCCG 1 cut(s) 256
Eam1104I CTCTTC 2 cut(s) 80, 296
EarI CTCTTC 2 cut(s) 80, 296
EclXI CGGCCG 1 cut(s) 256
Eco130I CCWWGG 2 cut(s) 212, 423
Eco47I GGWCC 1 cut(s) 471
Eco52I CGGCCG 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 215
EcoT14I CCWWGG 2 cut(s) 212, 423
ErhI CCWWGG 2 cut(s) 212, 423
FaeI CATG 2 cut(s) 235, 427
FaiI YATR 4 cut(s) 233, 304, 306, 425
FatI CATG 2 cut(s) 231, 423
FspBI CTAG 3 cut(s) 62, 267, 475
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 1 cut(s) 258
HapII CCGG 2 cut(s) 369, 462
HgaI GACGC 2 cut(s) 248, 454
Hin1I GRCGYC 3 cut(s) 135, 240, 465
Hin1II CATG 2 cut(s) 235, 427
HindIII AAGCTT 1 cut(s) 22
HinfI GANTC 1 cut(s) 325
HpaII CCGG 2 cut(s) 369, 462
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 1 cut(s) 394
Hpy188I TCNGA 2 cut(s) 150, 178
Hpy188III TCNNGA 3 cut(s) 62, 73, 169
Hpy8I GTNNAC 1 cut(s) 394
HpyAV CCTTC 2 cut(s) 50, 124
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 1 cut(s) 357
HpyF10VI GCNNNNNNNGC 1 cut(s) 270
HpyF3I CTNAG 1 cut(s) 418
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 3 cut(s) 135, 240, 465
Hsp92II CATG 2 cut(s) 235, 427
Kzo9I GATC 3 cut(s) 117, 180, 208
LmnI GCTCC 2 cut(s) 447, 464
LpnPI CCDG 5 cut(s) 86, 129, 249, 382, 454
LweI GCATC 1 cut(s) 260
MaeI CTAG 3 cut(s) 62, 267, 475
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 2 cut(s) 136, 278
MalI GATC 3 cut(s) 119, 182, 210
MboI GATC 3 cut(s) 117, 180, 208
MboII GAAGA 9 cut(s) 94, 97, 163, 208, 211, 236, 244, 283, 357
MflI RGATCY 1 cut(s) 180
MlyI GAGTC 1 cut(s) 334
MnlI CCTC 9 cut(s) 7, 80, 81, 194, 256, 416, 425, 442, 460
MseI TTAA 2 cut(s) 299, 383
MspI CCGG 2 cut(s) 369, 462
MwoI GCNNNNNNNGC 1 cut(s) 270
NcoI CCATGG 1 cut(s) 423
NdeII GATC 3 cut(s) 117, 180, 208
NlaIII CATG 2 cut(s) 235, 427
NlaIV GGNNCC 1 cut(s) 460
NmuCI GTSAC 1 cut(s) 136
NspI RCATGY 1 cut(s) 235
PaeI GCATGC 1 cut(s) 235
PcsI WCGNNNNNNNCGW 1 cut(s) 462
Pfl23II CGTACG 1 cut(s) 453
PleI GAGTC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 333
PspLI CGTACG 1 cut(s) 453
PspN4I GGNNCC 1 cut(s) 460
PspPI GGNCC 1 cut(s) 471
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 2 cut(s) 53, 455
RsaNI GTAC 2 cut(s) 52, 454
SaqAI TTAA 2 cut(s) 299, 383
Sau3AI GATC 3 cut(s) 117, 180, 208
Sau96I GGNCC 1 cut(s) 471
SchI GAGTC 1 cut(s) 334
SetI ASST 7 cut(s) 26, 39, 61, 128, 138, 248, 377
SfaNI GCATC 1 cut(s) 260
SinI GGWCC 1 cut(s) 471
SphI GCATGC 1 cut(s) 235
SspMI CTAG 3 cut(s) 62, 267, 475
StyI CCWWGG 2 cut(s) 212, 423
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 1 cut(s) 138
TaqI TCGA 2 cut(s) 120, 168
Tru1I TTAA 2 cut(s) 299, 383
Tru9I TTAA 2 cut(s) 299, 383
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 1 cut(s) 16
TspGWI ACGGA 1 cut(s) 339
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 471
XbaI TCTAGA 1 cut(s) 61
XceI RCATGY 1 cut(s) 235
XspI CTAG 3 cut(s) 62, 267, 475
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.