AT1G73500

kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001823
Physical Location & Seq
Reverse (-)
27639091 .. 27640643
1553 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G73500.1

Sequence Viewer

Length: 933 bp
ATGGCTTTAGTACGTGAACGTCGTCAGCTAAATCTCCGTCTTCCTCTTCCTCCAATCTCCGACCGCCGCTTCTCCACCTCTTCCTCCTCAGCCACCACCACCACCGTCGCTGGCTGTAACGGAATCTCCGCTTGTGATCTCGAGAAACTCAACGTTCTCGGATGCGGAAACGGCGGGATTGTTTACAAAGTCCGTCATAAAACCACATCGGAGATCTACGCTTTGAAAACAGTTAACGGCGACATGGATCCGATTTTCACAAGACAGTTGATGCGAGAGATGGAGATTCTCCGCCGTACAGATTCACCGTACGTCGTTAAGTGTCACGGAATCTTCGAGAAACCTGTCGTCGGTGAAGTATCGATTCTAATGGAGTATATGGACGGCGGAACCCTAGAATCACTACGCGGCGGTGTAACGGAGCAAAAACTCGCCGGATTCGCTAAACAGATCTTAAAAGGATTAAGCTATTTACACGCTCTTAAGATCGTTCATCGTGATATCAAACCGGCGAATCTTCTTCTCAATTCGAAAAACGAAGTTAAAATCGCTGATTTCGGAGTTAGTAAGATCTTAGTCCGATCATTAGATTCGTGTAATTCGTATGTTGGTACTTGTGCTTATATGAGTCCGGAGAGGTTTGATTCGGAATCTTCCGGTGGAAGCTCTGATATCTACGCCGGAGATATCTGGAGTTTCGGATTGATGATGCTTGAGCTTCTCGTTGGTCATTTTCCTTTGCTTCCGCCGGGACAGAGACCTGATTGGGCGACGTTGATGTGCGCGGTGTGTTTTGGAGAACCGCCGCGAGCGCCGGAGGGATGTTCTGAAGAGTTTAGGAGCTTCGTTGAGTGTTGTCTCCGTAAAGATTCGAGTAAGCGATGGACGGCGCCGCAGCTTCTTGCTCATCCTTTTCTCCGGGAAGATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

310

Amino Acids

34.35

Weight (kDa)

7.97

Isoelectric Point (pI)

58.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 48 - 306 1.4e-61 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 48 - 300 3.4e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 889
AccII CGCG 3 cut(s) 408, 785, 808
AccIII TCCGGA 1 cut(s) 631
AclI AACGTT 1 cut(s) 153
AclWI GGATC 2 cut(s) 242, 255
AcuI CTGAAG 1 cut(s) 849
AcyI GRCGYC 1 cut(s) 890
AfaI GTAC 4 cut(s) 12, 298, 311, 613
AfiI CCNNNNNNNGG 1 cut(s) 350
AflII CTTAAG 1 cut(s) 482
AgsI TTSAA 1 cut(s) 226
AluBI AGCT 6 cut(s) 28, 468, 666, 718, 843, 898
AluI AGCT 6 cut(s) 28, 468, 666, 718, 843, 898
Alw26I GTCTC 2 cut(s) 751, 863
AlwI GGATC 2 cut(s) 242, 255
Ama87I CYCGRG 1 cut(s) 140
Aor13HI TCCGGA 1 cut(s) 631
ApeKI GCWGC 1 cut(s) 895
AspLEI GCGC 3 cut(s) 785, 814, 892
AsuC2I CCSGG 2 cut(s) 750, 920
AsuHPI GGTGA 2 cut(s) 297, 365
AsuII TTCGAA 1 cut(s) 530
AvaI CYCGRG 1 cut(s) 140
BamHI GGATCC 1 cut(s) 247
BanI GGYRCC 1 cut(s) 889
BbsI GAAGAC 1 cut(s) 32
BbvCI CCTCAGC 1 cut(s) 88
BbvI GCAGC 1 cut(s) 907
BccI CCATC 2 cut(s) 274, 876
BceAI ACGGC 5 cut(s) 187, 253, 279, 400, 903
BcnI CCSGG 2 cut(s) 750, 920
BcoDI GTCTC 2 cut(s) 751, 863
BfaI CTAG 1 cut(s) 395
BfoI RGCGCY 2 cut(s) 815, 893
BfrI CTTAAG 1 cut(s) 482
BglII AGATCT 4 cut(s) 213, 450, 570, 925
BisI GCNGC 5 cut(s) 67, 409, 806, 893, 896
BlsI GCNGC 5 cut(s) 68, 410, 807, 894, 897
Bme1390I CCNGG 2 cut(s) 750, 920
BmeT110I CYCGRG 1 cut(s) 140
BmiI GGNNCC 3 cut(s) 249, 391, 891
BmrFI CCNGG 2 cut(s) 750, 920
BmsI GCATC 3 cut(s) 152, 261, 699
BpiI GAAGAC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 712
Bpu10I CCTNAGC 1 cut(s) 88
Bpu14I TTCGAA 1 cut(s) 530
BpuEI CTTGAG 1 cut(s) 734
BpuMI CCSGG 2 cut(s) 750, 920
Bsa29I ATCGAT 1 cut(s) 362
BsaAI YACGTR 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 890
BsaI GGTCTC 1 cut(s) 751
BsaWI WCCGGW 2 cut(s) 631, 656
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 1 cut(s) 350
Bse118I RCCGGY 1 cut(s) 508
BseAI TCCGGA 1 cut(s) 631
BseCI ATCGAT 1 cut(s) 362
BseGI GGATG 3 cut(s) 167, 827, 907
BseLI CCNNNNNNNGG 1 cut(s) 350
BseMII CTCAG 1 cut(s) 102
BseRI GAGGAG 1 cut(s) 76
BseXI GCAGC 1 cut(s) 907
Bsh1236I CGCG 3 cut(s) 408, 785, 808
Bsh1285I CGRYCG 1 cut(s) 64
BshNI GGYRCC 1 cut(s) 889
BshVI ATCGAT 1 cut(s) 362
BsiEI CGRYCG 1 cut(s) 64
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 8 cut(s) 435, 509, 632, 657, 681, 749, 815, 919
BsiWI CGTACG 1 cut(s) 309
BslFI GGGAC 1 cut(s) 765
BslI CCNNNNNNNGG 1 cut(s) 350
BsmAI GTCTC 2 cut(s) 751, 863
BsmFI GGGAC 1 cut(s) 765
Bso31I GGTCTC 1 cut(s) 751
BsoBI CYCGRG 1 cut(s) 140
Bsp119I TTCGAA 1 cut(s) 530
Bsp13I TCCGGA 1 cut(s) 631
Bsp143I GATC 8 cut(s) 136, 213, 247, 450, 486, 570, 581, 925
BspCNI CTCAG 1 cut(s) 101
BspDI ATCGAT 1 cut(s) 362
BspEI TCCGGA 1 cut(s) 631
BspFNI CGCG 3 cut(s) 408, 785, 808
BspLI GGNNCC 3 cut(s) 249, 391, 891
BspPI GGATC 2 cut(s) 242, 255
BspT104I TTCGAA 1 cut(s) 530
BspT107I GGYRCC 1 cut(s) 889
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 751
BsrFI RCCGGY 1 cut(s) 508
BssAI RCCGGY 1 cut(s) 508
BssMI GATC 8 cut(s) 136, 213, 247, 450, 486, 570, 581, 925
BssNI GRCGYC 1 cut(s) 890
Bst4CI ACNGT 4 cut(s) 106, 232, 267, 309
Bst6I CTCTTC 3 cut(s) 51, 85, 825
BstACI GRCGYC 1 cut(s) 890
BstAFI CTTAAG 1 cut(s) 482
BstBAI YACGTR 1 cut(s) 14
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 2 cut(s) 112, 810
BstDEI CTNAG 2 cut(s) 88, 574
BstF5I GGATG 3 cut(s) 167, 827, 907
BstFNI CGCG 3 cut(s) 408, 785, 808
BstH2I RGCGCY 2 cut(s) 815, 893
BstHHI GCGC 3 cut(s) 785, 814, 892
BstKTI GATC 8 cut(s) 139, 216, 250, 453, 489, 573, 584, 928
BstMAI GTCTC 2 cut(s) 751, 863
BstMBI GATC 8 cut(s) 136, 213, 247, 450, 486, 570, 581, 925
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 3 cut(s) 171, 440, 811
BstSCI CCNGG 2 cut(s) 748, 918
BstUI CGCG 3 cut(s) 408, 785, 808
BstV1I GCAGC 1 cut(s) 907
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 5 cut(s) 213, 247, 450, 570, 925
BstYI RGATCY 5 cut(s) 213, 247, 450, 570, 925
Bsu15I ATCGAT 1 cut(s) 362
BsuTUI ATCGAT 1 cut(s) 362
BtgZI GCGATG 1 cut(s) 895
BtsCI GGATG 3 cut(s) 167, 827, 907
Cac8I GCNNGC 2 cut(s) 112, 810
CfoI GCGC 3 cut(s) 785, 814, 892
Cfr10I RCCGGY 1 cut(s) 508
ClaI ATCGAT 1 cut(s) 362
Csp6I GTAC 4 cut(s) 11, 297, 310, 612
CviAII CATG 1 cut(s) 244
CviJI RGCY 9 cut(s) 5, 28, 92, 114, 468, 666, 718, 843, 898
CviKI_1 RGCY 9 cut(s) 5, 28, 92, 114, 468, 666, 718, 843, 898
CviQI GTAC 4 cut(s) 11, 297, 310, 612
DdeI CTNAG 2 cut(s) 88, 574
DinI GGCGCC 1 cut(s) 891
DpnI GATC 8 cut(s) 138, 215, 249, 452, 488, 572, 583, 927
DpnII GATC 8 cut(s) 136, 213, 247, 450, 486, 570, 581, 925
Eam1104I CTCTTC 3 cut(s) 51, 85, 825
EarI CTCTTC 3 cut(s) 51, 85, 825
EciI GGCGGA 3 cut(s) 281, 402, 735
Eco31I GGTCTC 1 cut(s) 751
Eco32I GATATC 3 cut(s) 502, 673, 688
Eco57I CTGAAG 1 cut(s) 849
Eco88I CYCGRG 1 cut(s) 140
EcoRV GATATC 3 cut(s) 502, 673, 688
EgeI GGCGCC 1 cut(s) 891
EheI GGCGCC 1 cut(s) 891
FaeI CATG 1 cut(s) 247
FaiI YATR 7 cut(s) 198, 245, 378, 380, 606, 624, 626
FaqI GGGAC 1 cut(s) 765
FatI CATG 1 cut(s) 243
FauI CCCGC 1 cut(s) 167
Fnu4HI GCNGC 5 cut(s) 67, 409, 806, 893, 896
FokI GGATG 3 cut(s) 174, 834, 894
Fsp4HI GCNGC 5 cut(s) 67, 409, 806, 893, 896
FspBI CTAG 1 cut(s) 395
GlaI GCGC 3 cut(s) 784, 813, 891
GluI GCNGC 5 cut(s) 67, 409, 806, 893, 896
GsuI CTGGAG 1 cut(s) 712
HaeII RGCGCY 2 cut(s) 815, 893
HapII CCGG 8 cut(s) 435, 509, 632, 657, 681, 749, 815, 919
HhaI GCGC 3 cut(s) 785, 814, 892
Hin1I GRCGYC 1 cut(s) 890
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 3 cut(s) 783, 812, 890
HinP1I GCGC 3 cut(s) 783, 812, 890
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HpaI GTTAAC 1 cut(s) 235
HpaII CCGG 8 cut(s) 435, 509, 632, 657, 681, 749, 815, 919
HphI GGTGA 2 cut(s) 297, 365
Hpy166II GTNNAC 3 cut(s) 17, 184, 235
Hpy188III TCNNGA 6 cut(s) 140, 142, 337, 497, 632, 691
Hpy8I GTNNAC 3 cut(s) 17, 184, 235
Hpy99I CGWCG 5 cut(s) 24, 110, 317, 353, 775
HpyCH4III ACNGT 4 cut(s) 106, 232, 267, 309
HpyCH4IV ACGT 5 cut(s) 13, 19, 153, 312, 773
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 440, 811
HpyF3I CTNAG 2 cut(s) 88, 574
HpySE526I ACGT 5 cut(s) 13, 19, 153, 312, 773
Hsp92I GRCGYC 1 cut(s) 890
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 3 cut(s) 783, 812, 890
KasI GGCGCC 1 cut(s) 889
Kpn2I TCCGGA 1 cut(s) 631
KspAI GTTAAC 1 cut(s) 235
Kzo9I GATC 8 cut(s) 136, 213, 247, 450, 486, 570, 581, 925
LmnI GCTCC 2 cut(s) 421, 840
Lsp1109I GCAGC 1 cut(s) 907
LweI GCATC 3 cut(s) 152, 261, 699
MaeI CTAG 1 cut(s) 395
MaeII ACGT 5 cut(s) 13, 19, 153, 312, 773
MaeIII GTNAC 3 cut(s) 116, 323, 415
MalI GATC 8 cut(s) 138, 215, 249, 452, 488, 572, 583, 927
MboI GATC 8 cut(s) 136, 213, 247, 450, 486, 570, 581, 925
MboII GAAGA 8 cut(s) 32, 38, 72, 325, 509, 512, 645, 842
MflI RGATCY 5 cut(s) 213, 247, 450, 570, 925
MluCI AATT 2 cut(s) 526, 598
Mly113I GGCGCC 1 cut(s) 890
MlyI GAGTC 1 cut(s) 637
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 7 cut(s) 54, 60, 88, 94, 97, 630, 811
MroI TCCGGA 1 cut(s) 631
MseI TTAA 6 cut(s) 234, 318, 455, 464, 483, 543
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 8 cut(s) 435, 509, 632, 657, 681, 749, 815, 919
MspR9I CCNGG 2 cut(s) 750, 920
MvnI CGCG 3 cut(s) 408, 785, 808
MwoI GCNNNNNNNGC 3 cut(s) 171, 440, 811
NarI GGCGCC 1 cut(s) 890
NciI CCSGG 2 cut(s) 750, 920
NdeII GATC 8 cut(s) 136, 213, 247, 450, 486, 570, 581, 925
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 3 cut(s) 249, 391, 891
NmuCI GTSAC 1 cut(s) 323
NspV TTCGAA 1 cut(s) 530
PaeR7I CTCGAG 1 cut(s) 140
PcsI WCGNNNNNNNCGW 3 cut(s) 19, 333, 599
Pfl23II CGTACG 1 cut(s) 309
PfoI TCCNGGA 1 cut(s) 918
PkrI GCNGC 5 cut(s) 68, 410, 807, 894, 897
PleI GAGTC 1 cut(s) 636
PluTI GGCGCC 1 cut(s) 893
PpsI GAGTC 1 cut(s) 636
Ppu21I YACGTR 1 cut(s) 14
Psp1406I AACGTT 1 cut(s) 153
PspLI CGTACG 1 cut(s) 309
PspN4I GGNNCC 3 cut(s) 249, 391, 891
PsuI RGATCY 5 cut(s) 213, 247, 450, 570, 925
RsaI GTAC 4 cut(s) 12, 298, 311, 613
RsaNI GTAC 4 cut(s) 11, 297, 310, 612
SaqAI TTAA 6 cut(s) 234, 318, 455, 464, 483, 543
SatI GCNGC 5 cut(s) 67, 409, 806, 893, 896
Sau3AI GATC 8 cut(s) 136, 213, 247, 450, 486, 570, 581, 925
SchI GAGTC 1 cut(s) 637
ScrFI CCNGG 2 cut(s) 750, 920
SfaNI GCATC 3 cut(s) 152, 261, 699
SfoI GGCGCC 1 cut(s) 891
Sfr274I CTCGAG 1 cut(s) 140
SfuI TTCGAA 1 cut(s) 530
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 3 cut(s) 140, 482, 713
SmoI CTYRAG 3 cut(s) 140, 482, 713
Sse9I AATT 2 cut(s) 526, 598
SspDI GGCGCC 1 cut(s) 889
SspMI CTAG 1 cut(s) 395
StyD4I CCNGG 2 cut(s) 748, 918
TaaI ACNGT 4 cut(s) 106, 232, 267, 309
TaiI ACGT 5 cut(s) 16, 22, 156, 315, 776
TaqI TCGA 5 cut(s) 141, 336, 362, 530, 872
TasI AATT 2 cut(s) 526, 598
TauI GCSGC 4 cut(s) 69, 411, 808, 895
Tru1I TTAA 6 cut(s) 234, 318, 455, 464, 483, 543
Tru9I TTAA 6 cut(s) 234, 318, 455, 464, 483, 543
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 1 cut(s) 895
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 1 cut(s) 482
TspGWI ACGGA 6 cut(s) 26, 135, 182, 342, 434, 851
Vha464I CTTAAG 1 cut(s) 482
XhoI CTCGAG 1 cut(s) 140
XspI CTAG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.