Rh7BG010700

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001823
Physical Location & Seq
Forward (+)
689517 .. 690494
978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG010700.1

Sequence Viewer

Length: 867 bp
ATGGCTCTCATCCGGGAACGCCGCCAGCTCAACCTCTGCAGCCTCCCCTTGCCGGAACCCTCCGAGCGTCGCCCACGCCACGGTAACGGCGGCACAGTCTACAAAGTCCGCCACAAGCGCACTGGAGCTAACTACGCCCTAAAAATAGTGAAAGGCGACTCCGACCCCACCGTCCGCCGCCAGCTCTACCGCGAAATAGAAATCCTCCGCCGCACGGACTCCCCCCACGTGGTTCACTGCAACGCCATAATCGAGAAGCCCACCGGAGACATCTGGATTCTCATGGAGTACATGGACTCGGGAACATTAGAGACCTCGCTCAAAGAAAACGGCACGTTCTCCGAGGCCAAGGTCGCTCATGTAGCTCGCCAAGTCCTCAACGGTCTCAATTACCTCCACACTCAGAAGATCATCCACCGTGACATCAAGCCAGCCAATCTGTTAGTCAACAGCAACATGGAAGTCAAGATTGCCGACTTCGGCGTCAGCAAAATGATGTGCCGAACTCTCGATAACTGTAACTCATACGTTGGGACTTGTGCTTACATGAGCCCGGAGAGATTTGACCCTGACACTTACGGTGGAAACTACAATGGATACGCCAGTGATATATGGAGCTTGGGTCTAACCCTCATGGAGCTTTACATGGGCCACTTTCCGTTCTTGCCTCCGGGTCAGAGACCCGACTGGGCGACCCTTATGTGCGCCATATGTTTCGGGGAGCCACCGGCCTTGCCGGAGGGGGTGTCGGAGGAGTTTAGGAGTTTTATGGAGTGTAGCTTGCAAAAGGAGTCGGGGAAGAGGTGGACGGCGGCTCAGCTTTTGACGCACCCGTTTGTTTGTAAAGATCCGAAAGCCATTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

288

Amino Acids

32.67

Weight (kDa)

8.52

Isoelectric Point (pI)

54.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 28 - 280 2.5e-63 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 28 - 275 1.3e-37 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 82 - 166 3.3e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 170, 482
AccI GTMKAC 1 cut(s) 99
AccII CGCG 1 cut(s) 192
AciI CCGC 9 cut(s) 22, 90, 109, 175, 178, 190, 208, 211, 812
AclWI GGATC 1 cut(s) 842
AcvI CACGTG 1 cut(s) 229
AcyI GRCGYC 1 cut(s) 483
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 4 cut(s) 52, 80, 214, 229
AluBI AGCT 8 cut(s) 28, 128, 184, 365, 618, 640, 780, 820
AluI AGCT 8 cut(s) 28, 128, 184, 365, 618, 640, 780, 820
Alw26I GTCTC 4 cut(s) 261, 305, 389, 673
AlwI GGATC 1 cut(s) 842
Ama87I CYCGRG 1 cut(s) 298
AoxI GGCC 3 cut(s) 345, 649, 729
ApeKI GCWGC 1 cut(s) 39
AspLEI GCGC 2 cut(s) 120, 707
AspS9I GGNCC 1 cut(s) 649
AsuC2I CCSGG 3 cut(s) 14, 554, 672
AvaI CYCGRG 1 cut(s) 298
BaeI ACNNNNGTAYC 2 cut(s) 589, 622
BanII GRGCYC 1 cut(s) 554
BbrPI CACGTG 1 cut(s) 229
BbvI GCAGC 1 cut(s) 51
BceAI ACGGC 3 cut(s) 103, 346, 825
BciVI GTATCC 1 cut(s) 590
BcnI CCSGG 3 cut(s) 14, 554, 672
BcoDI GTCTC 4 cut(s) 261, 305, 389, 673
BfmI CTRYAG 1 cut(s) 37
BfuI GTATCC 1 cut(s) 590
BisI GCNGC 6 cut(s) 22, 40, 91, 178, 211, 813
BlpI GCTNAGC 1 cut(s) 816
BlsI GCNGC 6 cut(s) 23, 41, 92, 179, 212, 814
Bme1390I CCNGG 3 cut(s) 14, 554, 672
BmeT110I CYCGRG 1 cut(s) 298
BmgT120I GGNCC 1 cut(s) 649
BmiI GGNNCC 2 cut(s) 57, 723
BmrFI CCNGG 3 cut(s) 14, 554, 672
BmrI ACTGGG 1 cut(s) 697
BmuI ACTGGG 1 cut(s) 697
BpmI CTGGAG 1 cut(s) 144
Bpu1102I GCTNAGC 1 cut(s) 816
BpuMI CCSGG 3 cut(s) 14, 554, 672
BsaAI YACGTR 1 cut(s) 229
BsaHI GRCGYC 1 cut(s) 483
BsaI GGTCTC 3 cut(s) 305, 389, 673
BsaJI CCNNGG 3 cut(s) 79, 342, 348
BsaWI WCCGGW 1 cut(s) 263
BsaXI ACNNNNNCTCC 4 cut(s) 607, 637, 731, 761
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 80, 214, 229
Bse118I RCCGGY 1 cut(s) 727
Bse1I ACTGG 3 cut(s) 127, 603, 692
BseDI CCNNGG 3 cut(s) 79, 342, 348
BseGI GGATG 2 cut(s) 9, 411
BseLI CCNNNNNNNGG 4 cut(s) 52, 80, 214, 229
BseMII CTCAG 2 cut(s) 416, 830
BseNI ACTGG 3 cut(s) 127, 603, 692
BseRI GAGGAG 1 cut(s) 767
BseXI GCAGC 1 cut(s) 51
Bsh1236I CGCG 1 cut(s) 192
BshFI GGCC 3 cut(s) 347, 651, 731
BsiHKCI CYCGRG 1 cut(s) 298
BsiSI CCGG 7 cut(s) 13, 53, 264, 554, 671, 728, 737
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 4 cut(s) 52, 80, 214, 229
BsmAI GTCTC 4 cut(s) 261, 305, 389, 673
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 3 cut(s) 347, 651, 731
Bso31I GGTCTC 3 cut(s) 305, 389, 673
BsoBI CYCGRG 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 554
Bsp143I GATC 2 cut(s) 408, 847
Bsp1720I GCTNAGC 1 cut(s) 816
BspACI CCGC 9 cut(s) 22, 90, 109, 175, 178, 190, 208, 211, 812
BspANI GGCC 3 cut(s) 347, 651, 731
BspCNI CTCAG 2 cut(s) 415, 829
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 2 cut(s) 57, 723
BspMAI CTGCAG 1 cut(s) 41
BspPI GGATC 1 cut(s) 842
BspTNI GGTCTC 3 cut(s) 305, 389, 673
BsrFI RCCGGY 1 cut(s) 727
BsrI ACTGG 3 cut(s) 127, 603, 692
BssAI RCCGGY 1 cut(s) 727
BssECI CCNNGG 3 cut(s) 79, 342, 348
BssMI GATC 2 cut(s) 408, 847
BssNI GRCGYC 1 cut(s) 483
BssT1I CCWWGG 1 cut(s) 348
Bst4CI ACNGT 7 cut(s) 83, 97, 172, 383, 419, 518, 581
Bst6I CTCTTC 1 cut(s) 794
BstACI GRCGYC 1 cut(s) 483
BstBAI YACGTR 1 cut(s) 229
BstC8I GCNNGC 5 cut(s) 26, 182, 367, 432, 782
BstDEI CTNAG 2 cut(s) 402, 816
BstDSI CCRYGG 1 cut(s) 79
BstF5I GGATG 2 cut(s) 9, 411
BstFNI CGCG 1 cut(s) 192
BstHHI GCGC 2 cut(s) 120, 707
BstKTI GATC 2 cut(s) 411, 850
BstMAI GTCTC 4 cut(s) 261, 305, 389, 673
BstMBI GATC 2 cut(s) 408, 847
BstMWI GCNNNNNNNGC 5 cut(s) 117, 134, 353, 362, 826
BstSCI CCNGG 3 cut(s) 12, 552, 670
BstSFI CTRYAG 1 cut(s) 37
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 847
BstYI RGATCY 1 cut(s) 847
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 3 cut(s) 347, 651, 731
BtgI CCRYGG 1 cut(s) 79
BtsCI GGATG 2 cut(s) 9, 411
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 3 cut(s) 120, 235, 610
Cac8I GCNNGC 5 cut(s) 26, 182, 367, 432, 782
CfoI GCGC 2 cut(s) 120, 707
Cfr10I RCCGGY 1 cut(s) 727
Cfr13I GGNCC 1 cut(s) 649
CseI GACGC 3 cut(s) 56, 472, 835
Csp6I GTAC 1 cut(s) 289
CspCI CAANNNNNGTGG 2 cut(s) 714, 749
CviAII CATG 7 cut(s) 283, 292, 359, 457, 547, 634, 646
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 2 cut(s) 402, 816
DpnI GATC 2 cut(s) 410, 849
DpnII GATC 2 cut(s) 408, 847
DrdI GACNNNNNNGTC 2 cut(s) 170, 482
DseDI GACNNNNNNGTC 2 cut(s) 170, 482
Eam1104I CTCTTC 1 cut(s) 794
EarI CTCTTC 1 cut(s) 794
EciI GGCGGA 3 cut(s) 98, 164, 197
Eco130I CCWWGG 1 cut(s) 348
Eco24I GRGCYC 1 cut(s) 554
Eco31I GGTCTC 3 cut(s) 305, 389, 673
Eco72I CACGTG 1 cut(s) 229
Eco88I CYCGRG 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 348
EcoT38I GRGCYC 1 cut(s) 554
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 7 cut(s) 286, 295, 362, 460, 550, 637, 649
FaqI GGGAC 1 cut(s) 547
FatI CATG 7 cut(s) 282, 291, 358, 456, 546, 633, 645
FauNDI CATATG 1 cut(s) 710
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 6 cut(s) 22, 40, 91, 178, 211, 813
FokI GGATG 1 cut(s) 398
FriOI GRGCYC 1 cut(s) 554
Fsp4HI GCNGC 6 cut(s) 22, 40, 91, 178, 211, 813
GlaI GCGC 2 cut(s) 119, 706
GluI GCNGC 6 cut(s) 22, 40, 91, 178, 211, 813
GsuI CTGGAG 1 cut(s) 144
HaeIII GGCC 3 cut(s) 347, 651, 731
HapII CCGG 7 cut(s) 13, 53, 264, 554, 671, 728, 737
HgaI GACGC 3 cut(s) 56, 472, 835
HhaI GCGC 2 cut(s) 120, 707
Hin1I GRCGYC 1 cut(s) 483
Hin1II CATG 7 cut(s) 286, 295, 362, 460, 550, 637, 649
Hin6I GCGC 2 cut(s) 118, 705
HinP1I GCGC 2 cut(s) 118, 705
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HinfI GANTC 5 cut(s) 158, 218, 277, 296, 791
HpaII CCGG 7 cut(s) 13, 53, 264, 554, 671, 728, 737
Hpy166II GTNNAC 4 cut(s) 100, 235, 448, 807
Hpy188I TCNGA 7 cut(s) 64, 163, 343, 405, 678, 751, 852
Hpy188III TCNNGA 5 cut(s) 253, 274, 300, 466, 509
Hpy8I GTNNAC 4 cut(s) 100, 235, 448, 807
Hpy99I CGWCG 1 cut(s) 72
HpyCH4III ACNGT 7 cut(s) 83, 97, 172, 383, 419, 518, 581
HpyCH4IV ACGT 3 cut(s) 228, 335, 528
HpyCH4V TGCA 3 cut(s) 39, 240, 784
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 134, 353, 362, 826
HpyF3I CTNAG 2 cut(s) 402, 816
HpySE526I ACGT 3 cut(s) 228, 335, 528
Hsp92I GRCGYC 1 cut(s) 483
Hsp92II CATG 7 cut(s) 286, 295, 362, 460, 550, 637, 649
HspAI GCGC 2 cut(s) 118, 705
Kzo9I GATC 2 cut(s) 408, 847
LmnI GCTCC 4 cut(s) 125, 615, 637, 721
Lsp1109I GCAGC 1 cut(s) 51
MaeII ACGT 3 cut(s) 228, 335, 528
MaeIII GTNAC 3 cut(s) 83, 419, 518
MalI GATC 2 cut(s) 410, 849
MboI GATC 2 cut(s) 408, 847
MboII GAAGA 2 cut(s) 418, 811
MflI RGATCY 1 cut(s) 847
MhlI GDGCHC 1 cut(s) 554
MluCI AATT 1 cut(s) 388
MlyI GAGTC 4 cut(s) 152, 212, 290, 800
MmeI TCCRAC 2 cut(s) 186, 729
MspI CCGG 7 cut(s) 13, 53, 264, 554, 671, 728, 737
MspR9I CCNGG 3 cut(s) 14, 554, 672
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 5 cut(s) 117, 134, 353, 362, 826
NciI CCSGG 3 cut(s) 14, 554, 672
NdeI CATATG 1 cut(s) 710
NdeII GATC 2 cut(s) 408, 847
NlaIII CATG 7 cut(s) 286, 295, 362, 460, 550, 637, 649
NlaIV GGNNCC 2 cut(s) 57, 723
NmuCI GTSAC 1 cut(s) 419
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 12
PkrI GCNGC 6 cut(s) 23, 41, 92, 179, 212, 814
PleI GAGTC 4 cut(s) 152, 212, 290, 799
PmaCI CACGTG 1 cut(s) 229
PmlI CACGTG 1 cut(s) 229
PpsI GAGTC 4 cut(s) 152, 212, 290, 799
Ppu21I YACGTR 1 cut(s) 229
PspCI CACGTG 1 cut(s) 229
PspN4I GGNNCC 2 cut(s) 57, 723
PspPI GGNCC 1 cut(s) 649
PstI CTGCAG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 847
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SatI GCNGC 6 cut(s) 22, 40, 91, 178, 211, 813
Sau3AI GATC 2 cut(s) 408, 847
Sau96I GGNCC 1 cut(s) 649
SchI GAGTC 4 cut(s) 152, 212, 290, 800
ScrFI CCNGG 3 cut(s) 14, 554, 672
SduI GDGCHC 1 cut(s) 554
SfcI CTRYAG 1 cut(s) 37
Sse9I AATT 1 cut(s) 388
SsiI CCGC 9 cut(s) 22, 90, 109, 175, 178, 190, 208, 211, 812
StyD4I CCNGG 3 cut(s) 12, 552, 670
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 7 cut(s) 83, 97, 172, 383, 419, 518, 581
TaiI ACGT 3 cut(s) 231, 338, 531
TaqI TCGA 2 cut(s) 252, 510
TasI AATT 1 cut(s) 388
TatI WGTACW 1 cut(s) 288
TauI GCSGC 5 cut(s) 24, 93, 180, 213, 815
TfiI GAWTC 1 cut(s) 277
TscAI CASTG 3 cut(s) 127, 242, 610
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 1 cut(s) 39
Tsp45I GTSAC 1 cut(s) 419
TspGWI ACGGA 2 cut(s) 230, 648
TspRI CASTG 3 cut(s) 127, 242, 610
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.