Rw5G025540

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001823
Physical Location & Seq
Forward (+)
36562030 .. 36563091
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025540.1

Sequence Viewer

Length: 1062 bp
ATGGCTCTTCTCCAACAACGTCGAAAGATCCCCTCCTTGTCTTTAGCCTCCACCGCCGTCCACCACCGCCCAATTCTCTCCCTACAAACCATCCCTCGCCCTGCCGTCACCACCACCATCACCTCCTCCTCTTCCACAGCACCCATCTCCGCCTCAGACCTAGAGCGACTCCAAGTCTTGGGCCACGGCAGCGGCGGCACCGTCTACAAGGTCCTTCACAAGCCCACTTCCACCCCTTACGCTCTCAAACAACTCCACTCGCCGGCTGCTGACTCCTCCTCCGATAACAACACACTTCAGAGTGAACTCGACATACTCCGCCGCACAACCGGCTCTCCCTACGTCGTCCACTGCCACGCCATCTTCGAGAAGCCCTCCGGAGACGTGTCGGTTCTCATGGAGTACATGGACTTGGGTTCTCTTGAAACCTTGCTCAAGTCCCAAGGTCCCTTCTCCGAAGAAAAGCTTGCCCCCATCGCACGCCAAGTCCTCGAAGGCCTCGACTACCTTCACAAGACTCACAAGATTGTTCATCGCGACATCAAGCCCGGGAATCTTTTAATGAACAACAAGAAGGAGGTCAAGATCGCCGACTTTGGTTGCAGTAGTGACGAGTCTTGCAGTTCCTCTGTGGGGACTTGTGCTTATATGAGCCCGGAGCGGTTTGATCCTGAGAGATACGGTGGAAGTCACAATGGCTATGCCAGTGATATATGGAGCTTTGGGGTGACTATAATGGAACTCTACATGGGTTACTTCCCCTTGCTCCCCAAGGGTCAGAGACCGGACTGGGCATCTCTTATGTGCGCTATCTGTTTCGGAGAGGTGCCGTGCTTGCCGGAGGGTGCGTCGGAAGACTTTAGGAGCTTTCTTGAGTGCTGCATGCACAAGGAGGCCTCCAAGAGGTGGACAGCGGCTCAACTTTTGACCCACCCGTTCGTCTGCAAGGCTCCATCCGAGGAATGCAAGCCAAACTTGTTCGACAAGAAAAGAAAACGTGATGATGATGGCGTTGAGCGAAGTGAGAGATCTCAAAAATCAATCAAGGTTGAAGTATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

353

Amino Acids

39.07

Weight (kDa)

7.18

Isoelectric Point (pI)

63.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 55 - 314 5.4e-59 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 55 - 309 2e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 197, 826
AccB7I CCANNNNNTGG 2 cut(s) 178, 906
AccBSI CCGCTC 1 cut(s) 661
AccI GTMKAC 1 cut(s) 204
AccII CGCG 1 cut(s) 537
AccIII TCCGGA 1 cut(s) 377
AciI CCGC 9 cut(s) 54, 67, 150, 192, 195, 319, 322, 661, 914
AclWI GGATC 2 cut(s) 22, 662
AcuI CTGAAG 1 cut(s) 281
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 5 cut(s) 178, 262, 633, 903, 906
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 2 cut(s) 425, 1052
AhdI GACNNNNNGTC 1 cut(s) 173
AjiI CACGTC 1 cut(s) 385
AluBI AGCT 3 cut(s) 466, 720, 867
AluI AGCT 3 cut(s) 466, 720, 867
Alw26I GTCTC 2 cut(s) 375, 775
AlwI GGATC 2 cut(s) 22, 662
Ama87I CYCGRG 1 cut(s) 548
Aor13HI TCCGGA 1 cut(s) 377
AoxI GGCC 3 cut(s) 181, 496, 894
ApeKI GCWGC 3 cut(s) 189, 266, 879
AspLEI GCGC 1 cut(s) 809
AspS9I GGNCC 3 cut(s) 181, 211, 446
AsuC2I CCSGG 3 cut(s) 549, 550, 656
AsuHPI GGTGA 3 cut(s) 100, 112, 739
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 2 cut(s) 211, 446
BanI GGYRCC 2 cut(s) 197, 826
BanII GRGCYC 1 cut(s) 656
BbsI GAAGAC 1 cut(s) 861
BbvI GCAGC 3 cut(s) 201, 253, 866
BccI CCATC 7 cut(s) 98, 125, 152, 368, 482, 961, 1001
BceAI ACGGC 4 cut(s) 41, 89, 202, 814
BcnI CCSGG 3 cut(s) 549, 550, 656
BcoDI GTCTC 2 cut(s) 375, 775
BfaI CTAG 1 cut(s) 161
BglII AGATCT 1 cut(s) 1028
BisI GCNGC 7 cut(s) 190, 193, 196, 267, 322, 880, 915
BlsI GCNGC 7 cut(s) 191, 194, 197, 268, 323, 881, 916
Bme1390I CCNGG 3 cut(s) 549, 550, 656
Bme18I GGWCC 2 cut(s) 211, 446
BmeRI GACNNNNNGTC 1 cut(s) 173
BmeT110I CYCGRG 1 cut(s) 548
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 3 cut(s) 181, 211, 446
BmiI GGNNCC 4 cut(s) 199, 448, 828, 951
BmrFI CCNGG 3 cut(s) 549, 550, 656
BmrI ACTGGG 1 cut(s) 799
BmsI GCATC 1 cut(s) 803
BmuI ACTGGG 1 cut(s) 799
BpiI GAAGAC 1 cut(s) 861
BplI GAGNNNNNCTC 2 cut(s) 359, 391
BpuEI CTTGAG 2 cut(s) 419, 893
BpuMI CCSGG 3 cut(s) 549, 550, 656
BsaI GGTCTC 1 cut(s) 775
BsaJI CCNNGG 5 cut(s) 184, 442, 548, 771, 957
BsaWI WCCGGW 2 cut(s) 377, 784
BsaXI ACNNNNNCTCC 6 cut(s) 263, 293, 319, 349, 833, 863
Bsc4I CCNNNNNNNGG 5 cut(s) 178, 262, 633, 903, 906
Bse118I RCCGGY 2 cut(s) 262, 329
Bse1I ACTGG 2 cut(s) 705, 794
BseAI TCCGGA 1 cut(s) 377
BseDI CCNNGG 5 cut(s) 184, 442, 548, 771, 957
BseGI GGATG 2 cut(s) 90, 953
BseLI CCNNNNNNNGG 5 cut(s) 178, 262, 633, 903, 906
BseMII CTCAG 2 cut(s) 168, 663
BseNI ACTGG 2 cut(s) 705, 794
BseRI GAGGAG 4 cut(s) 115, 118, 265, 268
BseXI GCAGC 3 cut(s) 201, 253, 866
Bsh1236I CGCG 1 cut(s) 537
BshFI GGCC 3 cut(s) 183, 498, 896
BshNI GGYRCC 2 cut(s) 197, 826
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 7 cut(s) 263, 330, 378, 549, 656, 785, 839
BslFI GGGAC 3 cut(s) 424, 432, 649
BslI CCNNNNNNNGG 5 cut(s) 178, 262, 633, 903, 906
BsmAI GTCTC 2 cut(s) 375, 775
BsmBI CGTCTC 1 cut(s) 375
BsmFI GGGAC 3 cut(s) 424, 432, 649
BsmI GAATGC 1 cut(s) 968
BsnI GGCC 3 cut(s) 183, 498, 896
Bso31I GGTCTC 1 cut(s) 775
BsoBI CYCGRG 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 656
Bsp13I TCCGGA 1 cut(s) 377
Bsp143I GATC 4 cut(s) 27, 585, 667, 1028
Bsp68I TCGCGA 1 cut(s) 537
BspACI CCGC 9 cut(s) 54, 67, 150, 192, 195, 319, 322, 661, 914
BspANI GGCC 3 cut(s) 183, 498, 896
BspCNI CTCAG 2 cut(s) 167, 664
BspEI TCCGGA 1 cut(s) 377
BspFNI CGCG 1 cut(s) 537
BspLI GGNNCC 4 cut(s) 199, 448, 828, 951
BspPI GGATC 2 cut(s) 22, 662
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 197, 826
BspTNI GGTCTC 1 cut(s) 775
BsrBI CCGCTC 1 cut(s) 661
BsrFI RCCGGY 2 cut(s) 262, 329
BsrI ACTGG 2 cut(s) 705, 794
BssAI RCCGGY 2 cut(s) 262, 329
BssECI CCNNGG 5 cut(s) 184, 442, 548, 771, 957
BssMI GATC 4 cut(s) 27, 585, 667, 1028
BssT1I CCWWGG 2 cut(s) 442, 771
Bst4CI ACNGT 2 cut(s) 202, 683
Bst6I CTCTTC 2 cut(s) 12, 136
BstC8I GCNNGC 6 cut(s) 264, 468, 481, 836, 884, 968
BstDEI CTNAG 2 cut(s) 154, 672
BstDSI CCRYGG 1 cut(s) 184
BstENI CCTNNNNNAGG 1 cut(s) 901
BstF5I GGATG 2 cut(s) 90, 953
BstFNI CGCG 1 cut(s) 537
BstHHI GCGC 1 cut(s) 809
BstKTI GATC 4 cut(s) 30, 588, 670, 1031
BstMAI GTCTC 2 cut(s) 375, 775
BstMBI GATC 4 cut(s) 27, 585, 667, 1028
BstMWI GCNNNNNNNGC 6 cut(s) 53, 189, 195, 330, 476, 835
BstNSI RCATGY 1 cut(s) 886
BstSCI CCNGG 3 cut(s) 547, 548, 654
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 3 cut(s) 201, 253, 866
BstV2I GAAGAC 1 cut(s) 861
BstX2I RGATCY 2 cut(s) 27, 1028
BstYI RGATCY 2 cut(s) 27, 1028
BsuRI GGCC 3 cut(s) 183, 498, 896
BtgI CCRYGG 1 cut(s) 184
BtgZI GCGATG 2 cut(s) 460, 518
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 2 cut(s) 90, 953
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 349, 712
BtuMI TCGCGA 1 cut(s) 537
Cac8I GCNNGC 6 cut(s) 264, 468, 481, 836, 884, 968
CfoI GCGC 1 cut(s) 809
Cfr10I RCCGGY 2 cut(s) 262, 329
Cfr13I GGNCC 3 cut(s) 181, 211, 446
Cfr9I CCCGGG 1 cut(s) 548
CseI GACGC 1 cut(s) 837
Csp6I GTAC 1 cut(s) 403
CviAII CATG 4 cut(s) 397, 406, 748, 883
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 2 cut(s) 154, 672
DpnI GATC 4 cut(s) 29, 587, 669, 1030
DpnII GATC 4 cut(s) 27, 585, 667, 1028
DriI GACNNNNNGTC 1 cut(s) 173
Eam1104I CTCTTC 2 cut(s) 12, 136
Eam1105I GACNNNNNGTC 1 cut(s) 173
EarI CTCTTC 2 cut(s) 12, 136
EciI GGCGGA 2 cut(s) 139, 308
Eco130I CCWWGG 2 cut(s) 442, 771
Eco147I AGGCCT 2 cut(s) 498, 896
Eco24I GRGCYC 1 cut(s) 656
Eco31I GGTCTC 1 cut(s) 775
Eco47I GGWCC 2 cut(s) 211, 446
Eco57I CTGAAG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 548
EcoNI CCTNNNNNAGG 1 cut(s) 901
EcoO109I RGGNCCY 2 cut(s) 211, 446
EcoT14I CCWWGG 2 cut(s) 442, 771
EcoT38I GRGCYC 1 cut(s) 656
ErhI CCWWGG 2 cut(s) 442, 771
Esp3I CGTCTC 1 cut(s) 375
FaeI CATG 4 cut(s) 400, 409, 751, 886
FalI AAGNNNNNCTT 4 cut(s) 450, 482, 959, 991
FaqI GGGAC 3 cut(s) 424, 432, 649
FatI CATG 4 cut(s) 396, 405, 747, 882
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 7 cut(s) 190, 193, 196, 267, 322, 880, 915
FokI GGATG 2 cut(s) 77, 940
FriOI GRGCYC 1 cut(s) 656
Fsp4HI GCNGC 7 cut(s) 190, 193, 196, 267, 322, 880, 915
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 808
GluI GCNGC 7 cut(s) 190, 193, 196, 267, 322, 880, 915
HaeIII GGCC 3 cut(s) 183, 498, 896
HapII CCGG 7 cut(s) 263, 330, 378, 549, 656, 785, 839
HgaI GACGC 1 cut(s) 837
HhaI GCGC 1 cut(s) 809
Hin1II CATG 4 cut(s) 400, 409, 751, 886
Hin6I GCGC 1 cut(s) 807
HinP1I GCGC 1 cut(s) 807
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 5 cut(s) 168, 272, 517, 553, 614
HpaII CCGG 7 cut(s) 263, 330, 378, 549, 656, 785, 839
HphI GGTGA 3 cut(s) 100, 112, 739
Hpy166II GTNNAC 5 cut(s) 61, 205, 305, 349, 909
Hpy188I TCNGA 8 cut(s) 157, 283, 300, 457, 780, 821, 853, 958
Hpy188III TCNNGA 7 cut(s) 367, 378, 422, 536, 583, 671, 872
Hpy8I GTNNAC 5 cut(s) 61, 205, 305, 349, 909
Hpy99I CGWCG 3 cut(s) 24, 347, 853
HpyAV CCTTC 5 cut(s) 224, 460, 488, 518, 568
HpyCH4III ACNGT 2 cut(s) 202, 683
HpyCH4IV ACGT 4 cut(s) 19, 342, 384, 997
HpyCH4V TGCA 6 cut(s) 603, 621, 882, 886, 945, 966
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 189, 195, 330, 476, 835
HpyF3I CTNAG 2 cut(s) 154, 672
HpySE526I ACGT 4 cut(s) 19, 342, 384, 997
Hsp92II CATG 4 cut(s) 400, 409, 751, 886
HspAI GCGC 1 cut(s) 807
Kpn2I TCCGGA 1 cut(s) 377
KroI GCCGGC 1 cut(s) 262
KroNI GCCGGC 1 cut(s) 264
Kzo9I GATC 4 cut(s) 27, 585, 667, 1028
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 5 cut(s) 658, 717, 771, 864, 955
Lsp1109I GCAGC 3 cut(s) 201, 253, 866
LweI GCATC 1 cut(s) 803
MaeI CTAG 1 cut(s) 161
MaeII ACGT 4 cut(s) 19, 342, 384, 997
MaeIII GTNAC 5 cut(s) 106, 608, 689, 727, 752
MalI GATC 4 cut(s) 29, 587, 669, 1030
MbiI CCGCTC 1 cut(s) 661
MboI GATC 4 cut(s) 27, 585, 667, 1028
MboII GAAGA 4 cut(s) 123, 355, 470, 866
MflI RGATCY 2 cut(s) 27, 1028
MhlI GDGCHC 1 cut(s) 656
MluCI AATT 1 cut(s) 72
MlyI GAGTC 4 cut(s) 162, 266, 511, 623
MmeI TCCRAC 2 cut(s) 37, 831
MroI TCCGGA 1 cut(s) 377
MroNI GCCGGC 1 cut(s) 262
MseI TTAA 1 cut(s) 560
MspA1I CMGCKG 2 cut(s) 192, 914
MspI CCGG 7 cut(s) 263, 330, 378, 549, 656, 785, 839
MspR9I CCNGG 3 cut(s) 549, 550, 656
Mva1269I GAATGC 1 cut(s) 968
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 6 cut(s) 53, 189, 195, 330, 476, 835
NaeI GCCGGC 1 cut(s) 264
NciI CCSGG 3 cut(s) 549, 550, 656
NdeII GATC 4 cut(s) 27, 585, 667, 1028
NgoMIV GCCGGC 1 cut(s) 262
NlaIII CATG 4 cut(s) 400, 409, 751, 886
NlaIV GGNNCC 4 cut(s) 199, 448, 828, 951
NmuCI GTSAC 4 cut(s) 106, 608, 689, 727
NruI TCGCGA 1 cut(s) 537
NspI RCATGY 1 cut(s) 886
PaeI GCATGC 1 cut(s) 886
PceI AGGCCT 2 cut(s) 498, 896
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 498
PctI GAATGC 1 cut(s) 968
PdiI GCCGGC 1 cut(s) 264
PfeI GAWTC 1 cut(s) 553
PflMI CCANNNNNTGG 2 cut(s) 178, 906
PkrI GCNGC 7 cut(s) 191, 194, 197, 268, 323, 881, 916
PleI GAGTC 4 cut(s) 162, 266, 511, 622
PpsI GAGTC 4 cut(s) 162, 266, 511, 622
PpuMI RGGWCCY 2 cut(s) 211, 446
Psp5II RGGWCCY 2 cut(s) 211, 446
PspN4I GGNNCC 4 cut(s) 199, 448, 828, 951
PspPI GGNCC 3 cut(s) 181, 211, 446
PspPPI RGGWCCY 2 cut(s) 211, 446
PsrI GAACNNNNNNTAC 2 cut(s) 297, 329
PsuI RGATCY 2 cut(s) 27, 1028
RruI TCGCGA 1 cut(s) 537
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 560
SatI GCNGC 7 cut(s) 190, 193, 196, 267, 322, 880, 915
Sau3AI GATC 4 cut(s) 27, 585, 667, 1028
Sau96I GGNCC 3 cut(s) 181, 211, 446
SchI GAGTC 4 cut(s) 162, 266, 511, 623
ScrFI CCNGG 3 cut(s) 549, 550, 656
SduI GDGCHC 1 cut(s) 656
SfaNI GCATC 1 cut(s) 803
SinI GGWCC 2 cut(s) 211, 446
SmaI CCCGGG 1 cut(s) 550
SmlI CTYRAG 2 cut(s) 434, 872
SmoI CTYRAG 2 cut(s) 434, 872
SphI GCATGC 1 cut(s) 886
Sse9I AATT 1 cut(s) 72
SseBI AGGCCT 2 cut(s) 498, 896
SsiI CCGC 9 cut(s) 54, 67, 150, 192, 195, 319, 322, 661, 914
SspMI CTAG 1 cut(s) 161
StuI AGGCCT 2 cut(s) 498, 896
StyD4I CCNGG 3 cut(s) 547, 548, 654
StyI CCWWGG 2 cut(s) 442, 771
TaaI ACNGT 2 cut(s) 202, 683
TaiI ACGT 4 cut(s) 22, 345, 387, 1000
TaqI TCGA 6 cut(s) 22, 309, 366, 492, 501, 981
TasI AATT 1 cut(s) 72
TatI WGTACW 1 cut(s) 402
TauI GCSGC 4 cut(s) 195, 198, 324, 917
TfiI GAWTC 1 cut(s) 553
Tru1I TTAA 1 cut(s) 560
Tru9I TTAA 1 cut(s) 560
TscAI CASTG 2 cut(s) 356, 712
TseFI GTSAC 4 cut(s) 106, 608, 689, 727
TseI GCWGC 3 cut(s) 189, 266, 879
Tsp45I GTSAC 4 cut(s) 106, 608, 689, 727
TspDTI ATGAA 2 cut(s) 521, 578
TspMI CCCGGG 1 cut(s) 548
TspRI CASTG 2 cut(s) 356, 712
Van91I CCANNNNNTGG 2 cut(s) 178, 906
VpaK11BI GGWCC 2 cut(s) 211, 446
XagI CCTNNNNNAGG 1 cut(s) 901
XceI RCATGY 1 cut(s) 886
XmaI CCCGGG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.