Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001823

Physical Location & Seq

Genomic Coordinates Reverse (-)

23320649 .. 23321599

Gene Structure 951 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1228300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 951 bp

ATGGCTCTCATTCGAGGTCGGCAACTGAAGTCCTTGGCCCCTCTCTCCGTGCACGTGCACCGCCCATGTTTCTCTCTTCCCGTCCATCCTCCCGCACTGATGACAGCAACCAACTACTCCTCCACTTCAATCTCCACTGCTGATCTCGAGACACTCGAACTCCTCGGCCAAGGTAATGGCGGCAAGGTCTACAAGGTTCGCCACAAGCAGACTTCCAGTACCTACGCTCTCAAACTCGTCAGCAACCATTCAGACCATACGGTCAGGTGTAATCTCTCCCGCGAGATTGAGATCCTCCGCTGCACCGACTCTTCCCACATTGTCCGCTGTGAAGCCTTCTTTGAACAGCCCTTGGGAGACATCAAAATCCTCATGGAGTTCATGGACTCCGGCAGCCTCGAAACTTTACTCAAAGACAATGGCACCTTCTCCGAGGCCAAGCTTGCCCACGTGGCCCGTCAAGTGCTCAGCGGCCTGAACTATCTCCATACCCACAACATCGCTCACCGTGACATCAAGCCCGCCAACATTTTGGTGAACAGTAACATGGAAGTTAAGATCGCCGACTTCGGTATCAGCAAGATCATGTCCTTAACCTCGGAAGGCGCCTGCAGTTCTCCTGTCGGTACTTTCGCTTACATGAGCCCCGAAAGATTCAACAAGGAGAACTGTGGTGGCACCTACAGTGGCTACGCCAGTGACATATGGAGTTTCGGCCTGACCATCATGGAGCTCTACGTGGGTCGCTATCCACTCTTGCCCCCAGGCCAGAGTCCCGACTGGCCCTCCCTGATGTTAGCCATATGTTTTGGAGAGCCGCCGAGCTTGCCGGAGGGCGCTTCGCAGGAATTCAAGAGTTTTATTGAGGCTTGCTTGCAGAAGGAAGTCGGAAAGAGGTGGACTGCCGGTCAGTTGTTGAAGCATCCCTTCCTTTCCGATGTTGGTTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

317

Amino Acids

34.82

Weight (kDa)

6.86

Isoelectric Point (pI)

51.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	50 - 311	4.8e-64	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	50 - 306	8.3e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001823)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18350 AT1G73500 AT3G06230
fragaria_vesca	FvH4_5g02450 FvH4_5g02452 FvH4_5g02470 FvH4_5g17690
malus_domestica	MD06G1232600.v1.1 MD13G1223400.v1.1 MD14G1239400.v1.1 MD16G1227900.v1.1 MD16G1228300.v1.1
prunus_persica	Prupe.1G058300_v2.0.a1 Prupe.1G082800_v2.0.a1 Prupe.1G088400_v2.0.a1 Prupe.1G191900_v2.0.a1 Prupe.5G236900_v2.0.a1
pyrus_communis	pycom13g19740
rosa_chinensis	RchiOBHm_Chr5g0040641 RchiOBHm_Chr7g0177961 RchiOBHm_Chr7g0197661
rosa_laevigata	RLG00000003920 RLG00000003933 RLG00000005487 RLG00000009687
rosa_multiflora	Rmu_sc0002154.1_g000010 Rmu_sc0003916.1_g000020 Rmu_sc0006732.1_g000005
rosa_roxburghii	Rroxscaffold_3G00258300 Rroxscaffold_3G00258400 Rroxscaffold_5G00339620 Rroxscaffold_5G00339630
rosa_rugosa	Rorug06G0411800 Rorug07G0040300 Rorug07G0042200
rosa_samantha	Rh4CG058000 Rh5BG277200 Rh7AG010700 Rh7BG010700 Rh7BG168700 Rh7CG011100 Rh7CG175000 Rh7DG010800 Rh7DG168300
rosa_wichuraiana	Rw5G025540 Rw7G000900 Rw7G014500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	260
AccB1I	GGYRCC	3 cut(s)	422, 605, 677
AccI	GTMKAC	1 cut(s)	189
AccII	CGCG	1 cut(s)	282
AciI	CCGC	9 cut(s)	61, 93, 180, 280, 298, 325, 471, 522, 818
AclWI	GGATC	1 cut(s)	286
AcoI	YGGCCR	1 cut(s)	166
AcsI	RAATTY	1 cut(s)	848
AcuI	CTGAAG	1 cut(s)	47
AcvI	CACGTG	2 cut(s)	55, 451
AcyI	GRCGYC	1 cut(s)	606
AfaI	GTAC	2 cut(s)	220, 628
AgsI	TTSAA	5 cut(s)	129, 344, 658, 853, 919
AhdI	GACNNNNNGTC	1 cut(s)	906
AjnI	CCWGG	1 cut(s)	763
AluBI	AGCT	3 cut(s)	442, 733, 825
AluI	AGCT	3 cut(s)	442, 733, 825
Alw21I	GWGCWC	4 cut(s)	54, 60, 468, 735
Alw26I	GTCTC	2 cut(s)	143, 351
Alw44I	GTGCAC	2 cut(s)	50, 56
AlwI	GGATC	1 cut(s)	286
Ama87I	CYCGRG	1 cut(s)	146
AoxI	GGCC	8 cut(s)	36, 166, 435, 453, 472, 715, 766, 782
ApaLI	GTGCAC	2 cut(s)	50, 56
ApeKI	GCWGC	2 cut(s)	300, 393
ApoI	RAATTY	1 cut(s)	848
AspLEI	GCGC	2 cut(s)	608, 839
AspS9I	GGNCC	3 cut(s)	37, 454, 783
AsuHPI	GGTGA	2 cut(s)	497, 547
AvaI	CYCGRG	1 cut(s)	146
BaeGI	GKGCMC	2 cut(s)	54, 60
BaeI	ACNNNNGTAYC	2 cut(s)	556, 589
BanI	GGYRCC	3 cut(s)	422, 605, 677
BanII	GRGCYC	2 cut(s)	647, 735
BbrPI	CACGTG	2 cut(s)	55, 451
Bbv12I	GWGCWC	4 cut(s)	54, 60, 468, 735
BbvI	GCAGC	2 cut(s)	287, 405
BccI	CCATC	2 cut(s)	93, 731
BciT130I	CCWGG	1 cut(s)	765
BcoDI	GTCTC	2 cut(s)	143, 351
BfmI	CTRYAG	2 cut(s)	610, 682
BfoI	RGCGCY	2 cut(s)	609, 840
BglI	GCCNNNNNGGC	1 cut(s)	452
BisI	GCNGC	5 cut(s)	181, 301, 394, 472, 818
BlpI	GCTNAGC	1 cut(s)	467
BlsI	GCNGC	5 cut(s)	182, 302, 395, 473, 819
Bme1390I	CCNGG	1 cut(s)	765
BmeRI	GACNNNNNGTC	1 cut(s)	906
BmeT110I	CYCGRG	1 cut(s)	146
BmgT120I	GGNCC	3 cut(s)	37, 454, 783
BmiI	GGNNCC	4 cut(s)	39, 424, 607, 679
BmrFI	CCNGG	1 cut(s)	765
BmsI	GCATC	1 cut(s)	931
Bpu1102I	GCTNAGC	1 cut(s)	467
BsaAI	YACGTR	3 cut(s)	55, 451, 739
BsaBI	GATNNNNATC	1 cut(s)	290
BsaHI	GRCGYC	1 cut(s)	606
BsaJI	CCNNGG	7 cut(s)	33, 163, 169, 351, 432, 597, 763
BsaXI	ACNNNNNCTCC	6 cut(s)	104, 134, 144, 174, 770, 800
Bse118I	RCCGGY	1 cut(s)	905
Bse1I	ACTGG	3 cut(s)	216, 696, 785
Bse8I	GATNNNNATC	1 cut(s)	290
BseBI	CCWGG	1 cut(s)	765
BseDI	CCNNGG	7 cut(s)	33, 163, 169, 351, 432, 597, 763
BseGI	GGATG	2 cut(s)	85, 922
BseJI	GATNNNNATC	1 cut(s)	290
BseMII	CTCAG	1 cut(s)	481
BseNI	ACTGG	3 cut(s)	216, 696, 785
BseRI	GAGGAG	2 cut(s)	109, 152
BseSI	GKGCMC	2 cut(s)	54, 60
BseXI	GCAGC	2 cut(s)	287, 405
BsgI	GTGCAG	1 cut(s)	286
Bsh1236I	CGCG	1 cut(s)	282
BshFI	GGCC	8 cut(s)	38, 168, 437, 455, 474, 717, 768, 784
BshNI	GGYRCC	3 cut(s)	422, 605, 677
BsiHKAI	GWGCWC	4 cut(s)	54, 60, 468, 735
BsiHKCI	CYCGRG	1 cut(s)	146
BsiSI	CCGG	3 cut(s)	390, 830, 906
BslFI	GGGAC	1 cut(s)	759
BsmAI	GTCTC	2 cut(s)	143, 351
BsmFI	GGGAC	1 cut(s)	759
BsnI	GGCC	8 cut(s)	38, 168, 437, 455, 474, 717, 768, 784
BsoBI	CYCGRG	1 cut(s)	146
Bsp1286I	GDGCHC	5 cut(s)	54, 60, 468, 647, 735
Bsp143I	GATC	4 cut(s)	142, 291, 558, 582
Bsp1720I	GCTNAGC	1 cut(s)	467
BspACI	CCGC	9 cut(s)	61, 93, 180, 280, 298, 325, 471, 522, 818
BspANI	GGCC	8 cut(s)	38, 168, 437, 455, 474, 717, 768, 784
BspCNI	CTCAG	1 cut(s)	480
BspFNI	CGCG	1 cut(s)	282
BspLI	GGNNCC	4 cut(s)	39, 424, 607, 679
BspMAI	CTGCAG	1 cut(s)	614
BspPI	GGATC	1 cut(s)	286
BspT107I	GGYRCC	3 cut(s)	422, 605, 677
BsrFI	RCCGGY	1 cut(s)	905
BsrI	ACTGG	3 cut(s)	216, 696, 785
BssAI	RCCGGY	1 cut(s)	905
BssECI	CCNNGG	7 cut(s)	33, 163, 169, 351, 432, 597, 763
BssMI	GATC	4 cut(s)	142, 291, 558, 582
BssNI	GRCGYC	1 cut(s)	606
BssT1I	CCWWGG	3 cut(s)	33, 169, 351
Bst2UI	CCWGG	1 cut(s)	765
Bst4CI	ACNGT	5 cut(s)	262, 509, 542, 671, 686
Bst6I	CTCTTC	2 cut(s)	81, 316
BstACI	GRCGYC	1 cut(s)	606
BstBAI	YACGTR	3 cut(s)	55, 451, 739
BstC8I	GCNNGC	6 cut(s)	444, 522, 610, 827, 871, 875
BstDEI	CTNAG	1 cut(s)	467
BstF5I	GGATG	2 cut(s)	85, 922
BstFNI	CGCG	1 cut(s)	282
BstH2I	RGCGCY	2 cut(s)	609, 840
BstHHI	GCGC	2 cut(s)	608, 839
BstKTI	GATC	4 cut(s)	145, 294, 561, 585
BstMAI	GTCTC	2 cut(s)	143, 351
BstMBI	GATC	4 cut(s)	142, 291, 558, 582
BstMWI	GCNNNNNNNGC	3 cut(s)	443, 452, 826
BstNI	CCWGG	1 cut(s)	765
BstSCI	CCNGG	1 cut(s)	763
BstSFI	CTRYAG	2 cut(s)	610, 682
BstSLI	GKGCMC	2 cut(s)	54, 60
BstUI	CGCG	1 cut(s)	282
BstV1I	GCAGC	2 cut(s)	287, 405
BstX2I	RGATCY	1 cut(s)	291
BstXI	CCANNNNNNTGG	2 cut(s)	176, 532
BstYI	RGATCY	1 cut(s)	291
BsuRI	GGCC	8 cut(s)	38, 168, 437, 455, 474, 717, 768, 784
BtgZI	GCGATG	1 cut(s)	484
BtsCI	GGATG	2 cut(s)	85, 922
BtsI	GCAGTG	1 cut(s)	135
BtsIMutI	CAGTG	4 cut(s)	95, 135, 691, 703
Cac8I	GCNNGC	6 cut(s)	444, 522, 610, 827, 871, 875
CfoI	GCGC	2 cut(s)	608, 839
Cfr10I	RCCGGY	1 cut(s)	905
Cfr13I	GGNCC	3 cut(s)	37, 454, 783
Csp6I	GTAC	2 cut(s)	219, 627
CviAII	CATG	7 cut(s)	66, 373, 382, 547, 586, 640, 727
CviQI	GTAC	2 cut(s)	219, 627
DdeI	CTNAG	1 cut(s)	467
DinI	GGCGCC	1 cut(s)	607
DpnI	GATC	4 cut(s)	144, 293, 560, 584
DpnII	GATC	4 cut(s)	142, 291, 558, 582
DrdI	GACNNNNNNGTC	1 cut(s)	260
DriI	GACNNNNNGTC	1 cut(s)	906
DseDI	GACNNNNNNGTC	1 cut(s)	260
EaeI	YGGCCR	1 cut(s)	166
Eam1104I	CTCTTC	2 cut(s)	81, 316
Eam1105I	GACNNNNNGTC	1 cut(s)	906
EarI	CTCTTC	2 cut(s)	81, 316
Ecl136II	GAGCTC	1 cut(s)	733
Eco130I	CCWWGG	3 cut(s)	33, 169, 351
Eco24I	GRGCYC	2 cut(s)	647, 735
Eco53kI	GAGCTC	1 cut(s)	733
Eco57I	CTGAAG	1 cut(s)	47
Eco72I	CACGTG	2 cut(s)	55, 451
Eco88I	CYCGRG	1 cut(s)	146
EcoICRI	GAGCTC	1 cut(s)	733
EcoRI	GAATTC	1 cut(s)	848
EcoRII	CCWGG	1 cut(s)	763
EcoT14I	CCWWGG	3 cut(s)	33, 169, 351
EcoT38I	GRGCYC	2 cut(s)	647, 735
EgeI	GGCGCC	1 cut(s)	607
EheI	GGCGCC	1 cut(s)	607
ErhI	CCWWGG	3 cut(s)	33, 169, 351
FaeI	CATG	7 cut(s)	69, 376, 385, 550, 589, 643, 730
FalI	AAGNNNNNCTT	2 cut(s)	911, 943
FaqI	GGGAC	1 cut(s)	759
FatI	CATG	7 cut(s)	65, 372, 381, 546, 585, 639, 726
FauI	CCCGC	3 cut(s)	100, 287, 529
FauNDI	CATATG	2 cut(s)	704, 803
FblI	GTMKAC	1 cut(s)	189
Fnu4HI	GCNGC	5 cut(s)	181, 301, 394, 472, 818
FokI	GGATG	2 cut(s)	72, 909
FriOI	GRGCYC	2 cut(s)	647, 735
Fsp4HI	GCNGC	5 cut(s)	181, 301, 394, 472, 818
GlaI	GCGC	2 cut(s)	607, 838
GluI	GCNGC	5 cut(s)	181, 301, 394, 472, 818
HaeII	RGCGCY	2 cut(s)	609, 840
HaeIII	GGCC	8 cut(s)	38, 168, 437, 455, 474, 717, 768, 784
HapII	CCGG	3 cut(s)	390, 830, 906
HhaI	GCGC	2 cut(s)	608, 839
Hin1I	GRCGYC	1 cut(s)	606
Hin1II	CATG	7 cut(s)	69, 376, 385, 550, 589, 643, 730
Hin6I	GCGC	2 cut(s)	606, 837
HinP1I	GCGC	2 cut(s)	606, 837
HindIII	AAGCTT	1 cut(s)	440
HinfI	GANTC	4 cut(s)	308, 386, 654, 772
HpaII	CCGG	3 cut(s)	390, 830, 906
HphI	GGTGA	2 cut(s)	497, 547
Hpy166II	GTNNAC	5 cut(s)	52, 58, 190, 538, 900
Hpy188I	TCNGA	5 cut(s)	253, 433, 601, 890, 937
Hpy188III	TCNNGA	4 cut(s)	146, 148, 776, 853
Hpy8I	GTNNAC	5 cut(s)	52, 58, 190, 538, 900
HpyAV	CCTTC	5 cut(s)	346, 436, 596, 874, 937
HpyCH4III	ACNGT	5 cut(s)	262, 509, 542, 671, 686
HpyCH4IV	ACGT	3 cut(s)	54, 450, 738
HpyCH4V	TGCA	5 cut(s)	52, 58, 303, 612, 877
HpyF10VI	GCNNNNNNNGC	3 cut(s)	443, 452, 826
HpyF3I	CTNAG	1 cut(s)	467
HpySE526I	ACGT	3 cut(s)	54, 450, 738
Hsp92I	GRCGYC	1 cut(s)	606
Hsp92II	CATG	7 cut(s)	69, 376, 385, 550, 589, 643, 730
HspAI	GCGC	2 cut(s)	606, 837
KasI	GGCGCC	1 cut(s)	605
Kzo9I	GATC	4 cut(s)	142, 291, 558, 582
LmnI	GCTCC	1 cut(s)	730
Lsp1109I	GCAGC	2 cut(s)	287, 405
LweI	GCATC	1 cut(s)	931
MaeII	ACGT	3 cut(s)	54, 450, 738
MaeIII	GTNAC	3 cut(s)	509, 542, 698
MalI	GATC	4 cut(s)	144, 293, 560, 584
MboI	GATC	4 cut(s)	142, 291, 558, 582
MboII	GAAGA	2 cut(s)	68, 303
MflI	RGATCY	1 cut(s)	291
MhlI	GDGCHC	5 cut(s)	54, 60, 468, 647, 735
MluCI	AATT	1 cut(s)	848
Mly113I	GGCGCC	1 cut(s)	606
MlyI	GAGTC	3 cut(s)	302, 380, 781
MmeI	TCCRAC	1 cut(s)	868
MseI	TTAA	2 cut(s)	555, 593
MslI	CAYNNNNRTG	1 cut(s)	533
MspA1I	CMGCKG	3 cut(s)	300, 327, 471
MspI	CCGG	3 cut(s)	390, 830, 906
MspR9I	CCNGG	1 cut(s)	765
MvaI	CCWGG	1 cut(s)	765
MvnI	CGCG	1 cut(s)	282
MwoI	GCNNNNNNNGC	3 cut(s)	443, 452, 826
NarI	GGCGCC	1 cut(s)	606
NdeI	CATATG	2 cut(s)	704, 803
NdeII	GATC	4 cut(s)	142, 291, 558, 582
NlaIII	CATG	7 cut(s)	69, 376, 385, 550, 589, 643, 730
NlaIV	GGNNCC	4 cut(s)	39, 424, 607, 679
NmeAIII	GCCGAG	2 cut(s)	144, 846
NmuCI	GTSAC	2 cut(s)	509, 698
PaeR7I	CTCGAG	1 cut(s)	146
PcsI	WCGNNNNNNNCGW	1 cut(s)	153
PfeI	GAWTC	1 cut(s)	654
PkrI	GCNGC	5 cut(s)	182, 302, 395, 473, 819
PleI	GAGTC	3 cut(s)	302, 380, 780
PluTI	GGCGCC	1 cut(s)	609
PmaCI	CACGTG	2 cut(s)	55, 451
PmlI	CACGTG	2 cut(s)	55, 451
PpsI	GAGTC	3 cut(s)	302, 380, 780
Ppu21I	YACGTR	3 cut(s)	55, 451, 739
Psp124BI	GAGCTC	1 cut(s)	735
Psp6I	CCWGG	1 cut(s)	763
PspCI	CACGTG	2 cut(s)	55, 451
PspGI	CCWGG	1 cut(s)	763
PspN4I	GGNNCC	4 cut(s)	39, 424, 607, 679
PspPI	GGNCC	3 cut(s)	37, 454, 783
PstI	CTGCAG	1 cut(s)	614
PsuI	RGATCY	1 cut(s)	291
RsaI	GTAC	2 cut(s)	220, 628
RsaNI	GTAC	2 cut(s)	219, 627
RseI	CAYNNNNRTG	1 cut(s)	533
SacI	GAGCTC	1 cut(s)	735
SaqAI	TTAA	2 cut(s)	555, 593
SatI	GCNGC	5 cut(s)	181, 301, 394, 472, 818
Sau3AI	GATC	4 cut(s)	142, 291, 558, 582
Sau96I	GGNCC	3 cut(s)	37, 454, 783
SchI	GAGTC	3 cut(s)	302, 380, 781
ScrFI	CCNGG	1 cut(s)	765
SduI	GDGCHC	5 cut(s)	54, 60, 468, 647, 735
SfaNI	GCATC	1 cut(s)	931
SfcI	CTRYAG	2 cut(s)	610, 682
SfoI	GGCGCC	1 cut(s)	607
Sfr274I	CTCGAG	1 cut(s)	146
SlaI	CTCGAG	1 cut(s)	146
SmiMI	CAYNNNNRTG	1 cut(s)	533
SmlI	CTYRAG	1 cut(s)	146
SmoI	CTYRAG	1 cut(s)	146
Sse9I	AATT	1 cut(s)	848
SsiI	CCGC	9 cut(s)	61, 93, 180, 280, 298, 325, 471, 522, 818
SspDI	GGCGCC	1 cut(s)	605
SstI	GAGCTC	1 cut(s)	735
StyD4I	CCNGG	1 cut(s)	763
StyI	CCWWGG	3 cut(s)	33, 169, 351
TaaI	ACNGT	5 cut(s)	262, 509, 542, 671, 686
TaiI	ACGT	3 cut(s)	57, 453, 741
TaqI	TCGA	4 cut(s)	13, 147, 156, 399
TasI	AATT	1 cut(s)	848
TauI	GCSGC	3 cut(s)	183, 474, 820
TfiI	GAWTC	1 cut(s)	654
Tru1I	TTAA	2 cut(s)	555, 593
Tru9I	TTAA	2 cut(s)	555, 593
TscAI	CASTG	4 cut(s)	102, 142, 691, 703
TseFI	GTSAC	2 cut(s)	509, 698
TseI	GCWGC	2 cut(s)	300, 393
Tsp45I	GTSAC	2 cut(s)	509, 698
TspDTI	ATGAA	1 cut(s)	370
TspGWI	ACGGA	1 cut(s)	37
TspRI	CASTG	4 cut(s)	102, 142, 691, 703
VneI	GTGCAC	2 cut(s)	50, 56
XapI	RAATTY	1 cut(s)	848
XhoI	CTCGAG	1 cut(s)	146
XmiI	GTMKAC	1 cut(s)	189

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.