MD16G1227900.v1.1

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001823
Physical Location & Seq
Reverse (-)
23182496 .. 23182852
357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1227900.v1.1.491

Sequence Viewer

Length: 357 bp
ATGGCTCTCATTCGAGGTCGGCAACTGAAGTCCTTGGCCCCTCTCTCCGTGCACGAGCACTGCGCATGTTTCTCTCTTCCCGTCCATCCTCCCGCACTGATGACAGACACCAACTACTCCTCCACTTCAATCTCCGCTGCTGATCTCGAGACACTAGAACTCCTCGGCCAAGGTAATGGCGGCAAGGTCTACAAGGTTCGCCACAAGCAGACTTCCAGTACCTACGCTCTCAAACTCGTCAGCAACCATTCAGACCCCACGGTCAGATGTAATCTCTTCCGTGAGATTGAGATCCTCCGCTGCACTGACTTTCCCCACATTGTCCGCTGTGAAGCCTTCTTTGAACAGTCCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

119

Amino Acids

13.16

Weight (kDa)

7.7

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 50 - 116 2.6e-12 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
Acc16I TGCGCA 1 cut(s) 64
AccI GTMKAC 1 cut(s) 189
AciI CCGC 5 cut(s) 93, 135, 180, 298, 325
AclWI GGATC 1 cut(s) 286
AcoI YGGCCR 1 cut(s) 166
AcuI CTGAAG 1 cut(s) 47
AfaI GTAC 1 cut(s) 220
AgsI TTSAA 2 cut(s) 129, 344
Alw21I GWGCWC 2 cut(s) 54, 60
Alw26I GTCTC 1 cut(s) 143
Alw44I GTGCAC 1 cut(s) 50
AlwI GGATC 1 cut(s) 286
Ama87I CYCGRG 1 cut(s) 146
AoxI GGCC 2 cut(s) 36, 166
ApaLI GTGCAC 1 cut(s) 50
ApeKI GCWGC 2 cut(s) 137, 300
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 1 cut(s) 37
AvaI CYCGRG 1 cut(s) 146
BaeGI GKGCMC 1 cut(s) 54
BauI CACGAG 1 cut(s) 53
Bbv12I GWGCWC 2 cut(s) 54, 60
BbvI GCAGC 2 cut(s) 124, 287
BccI CCATC 1 cut(s) 93
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 1 cut(s) 155
BisI GCNGC 3 cut(s) 138, 181, 301
BlsI GCNGC 3 cut(s) 139, 182, 302
BmeT110I CYCGRG 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 39
BsaBI GATNNNNATC 1 cut(s) 290
BsaJI CCNNGG 4 cut(s) 33, 163, 169, 258
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 144, 174
Bse1I ACTGG 1 cut(s) 216
Bse8I GATNNNNATC 1 cut(s) 290
BseDI CCNNGG 4 cut(s) 33, 163, 169, 258
BseGI GGATG 1 cut(s) 85
BseJI GATNNNNATC 1 cut(s) 290
BseNI ACTGG 1 cut(s) 216
BseRI GAGGAG 2 cut(s) 109, 152
BseSI GKGCMC 1 cut(s) 54
BseXI GCAGC 2 cut(s) 124, 287
BsgI GTGCAG 1 cut(s) 286
BshFI GGCC 2 cut(s) 38, 168
BsiHKAI GWGCWC 2 cut(s) 54, 60
BsiHKCI CYCGRG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 143
BsnI GGCC 2 cut(s) 38, 168
BsoBI CYCGRG 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 54, 60
Bsp143I GATC 2 cut(s) 142, 291
BspACI CCGC 5 cut(s) 93, 135, 180, 298, 325
BspANI GGCC 2 cut(s) 38, 168
BspLI GGNNCC 1 cut(s) 39
BspPI GGATC 1 cut(s) 286
BsrI ACTGG 1 cut(s) 216
BssECI CCNNGG 4 cut(s) 33, 163, 169, 258
BssMI GATC 2 cut(s) 142, 291
BssSI CACGAG 1 cut(s) 53
BssT1I CCWWGG 2 cut(s) 33, 169
Bst2BI CACGAG 1 cut(s) 53
Bst4CI ACNGT 2 cut(s) 262, 348
Bst6I CTCTTC 2 cut(s) 81, 281
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 85
BstHHI GCGC 1 cut(s) 65
BstKTI GATC 2 cut(s) 145, 294
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 2 cut(s) 142, 291
BstNSI RCATGY 1 cut(s) 69
BstSLI GKGCMC 1 cut(s) 54
BstV1I GCAGC 2 cut(s) 124, 287
BstX2I RGATCY 1 cut(s) 291
BstXI CCANNNNNNTGG 1 cut(s) 176
BstYI RGATCY 1 cut(s) 291
BsuRI GGCC 2 cut(s) 38, 168
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 1 cut(s) 85
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 3 cut(s) 58, 95, 303
CfoI GCGC 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 37
Csp6I GTAC 1 cut(s) 219
CviAII CATG 1 cut(s) 66
CviJI RGCY 4 cut(s) 5, 38, 168, 335
CviKI_1 RGCY 4 cut(s) 5, 38, 168, 335
CviQI GTAC 1 cut(s) 219
DpnI GATC 2 cut(s) 144, 293
DpnII GATC 2 cut(s) 142, 291
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 166
Eam1104I CTCTTC 2 cut(s) 81, 281
EarI CTCTTC 2 cut(s) 81, 281
Eco130I CCWWGG 2 cut(s) 33, 169
Eco57I CTGAAG 1 cut(s) 47
Eco88I CYCGRG 1 cut(s) 146
EcoT14I CCWWGG 2 cut(s) 33, 169
ErhI CCWWGG 2 cut(s) 33, 169
FaeI CATG 1 cut(s) 69
FaiI YATR 1 cut(s) 67
FatI CATG 1 cut(s) 65
FauI CCCGC 1 cut(s) 100
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 3 cut(s) 138, 181, 301
FokI GGATG 1 cut(s) 72
Fsp4HI GCNGC 3 cut(s) 138, 181, 301
FspBI CTAG 1 cut(s) 155
FspI TGCGCA 1 cut(s) 64
GlaI GCGC 1 cut(s) 64
GluI GCNGC 3 cut(s) 138, 181, 301
HaeIII GGCC 2 cut(s) 38, 168
HhaI GCGC 1 cut(s) 65
Hin1II CATG 1 cut(s) 69
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
Hpy166II GTNNAC 2 cut(s) 52, 190
Hpy188I TCNGA 2 cut(s) 253, 266
Hpy188III TCNNGA 2 cut(s) 146, 148
Hpy8I GTNNAC 2 cut(s) 52, 190
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 2 cut(s) 262, 348
HpyCH4V TGCA 2 cut(s) 52, 303
Hsp92II CATG 1 cut(s) 69
HspAI GCGC 1 cut(s) 63
Kzo9I GATC 2 cut(s) 142, 291
LpnPI CCDG 1 cut(s) 229
Lsp1109I GCAGC 2 cut(s) 124, 287
MaeI CTAG 1 cut(s) 155
MalI GATC 2 cut(s) 144, 293
MboI GATC 2 cut(s) 142, 291
MboII GAAGA 2 cut(s) 68, 268
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 2 cut(s) 54, 60
MnlI CCTC 6 cut(s) 8, 51, 99, 130, 173, 305
MspA1I CMGCKG 3 cut(s) 137, 300, 327
NdeII GATC 2 cut(s) 142, 291
NlaIII CATG 1 cut(s) 69
NlaIV GGNNCC 1 cut(s) 39
NmeAIII GCCGAG 1 cut(s) 144
NsbI TGCGCA 1 cut(s) 64
NspI RCATGY 1 cut(s) 69
PaeR7I CTCGAG 1 cut(s) 146
PkrI GCNGC 3 cut(s) 139, 182, 302
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 1 cut(s) 37
PsuI RGATCY 1 cut(s) 291
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
SatI GCNGC 3 cut(s) 138, 181, 301
Sau3AI GATC 2 cut(s) 142, 291
Sau96I GGNCC 1 cut(s) 37
SduI GDGCHC 2 cut(s) 54, 60
SetI ASST 5 cut(s) 19, 175, 189, 198, 224
Sfr274I CTCGAG 1 cut(s) 146
SlaI CTCGAG 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SsiI CCGC 5 cut(s) 93, 135, 180, 298, 325
SspMI CTAG 1 cut(s) 155
StyI CCWWGG 2 cut(s) 33, 169
TaaI ACNGT 2 cut(s) 262, 348
TaqI TCGA 2 cut(s) 13, 147
TauI GCSGC 1 cut(s) 183
TscAI CASTG 3 cut(s) 65, 102, 310
TseI GCWGC 2 cut(s) 137, 300
TspGWI ACGGA 2 cut(s) 37, 269
TspRI CASTG 3 cut(s) 65, 102, 310
VneI GTGCAC 1 cut(s) 50
XceI RCATGY 1 cut(s) 69
XhoI CTCGAG 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.