pycom13g19740

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001823
Physical Location & Seq
Reverse (-)
15692131 .. 15692744
614 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g19740.1

Sequence Viewer

Length: 516 bp
ATGGCTCTCATTCGACGTCGGCAACTAAAGTCCTTGGCCTCTCTCTCCGATCACCGCATGTGTTTCTTTCTGCCCCTCTATCCTCCTGAACTCATAGCCGCTGCCAACTACTCTTCCGCTTCAATCTTTATCGCCAAACTCGAGACACTCGAACTCCTCGGCCAAGGTAATGGCAGCAAGATTGAAATCTTTCACCGCACTGACTCTCCCCACATTGTCCGCTGTAAAGCCTTATTTGAACAGCTCTTGGGAGATATCAAGATCCTTATGGGGTTCATGGACTCGGGCACCCTCGAAACTTTACTCATAGACAATGGCGCGTTCTCTGAGGCCAAGCTTGCCCACGTGCCACGTCAAGTTCTCAATGACCAGAACTATCTCCACACGCACAACATCACTCATCGTGCCAATCTTTTGGTGAACAGTAACATGGAAGTGAAGATCCCCGACTTTGGCATAAGCAAGATCGTTCCCTTAACCTCCTACGGTGCCTGTAGTTCTCCCGTCGGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

172

Amino Acids

18.93

Weight (kDa)

7.79

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 60 - 157 1.7e-11 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 61 - 161 7.4e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 19
AccB1I GGYRCC 2 cut(s) 287, 488
AccII CGCG 1 cut(s) 320
AciI CCGC 5 cut(s) 55, 99, 117, 196, 220
AclWI GGATC 2 cut(s) 256, 436
AcoI YGGCCR 1 cut(s) 160
AcvI CACGTG 1 cut(s) 346
AcyI GRCGYC 1 cut(s) 16
AfaI GTAC 1 cut(s) 511
AfiI CCNNNNNNNGG 1 cut(s) 452
AgsI TTSAA 3 cut(s) 123, 185, 239
AjiI CACGTC 1 cut(s) 353
AluBI AGCT 2 cut(s) 244, 337
AluI AGCT 2 cut(s) 244, 337
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 2 cut(s) 256, 436
Ama87I CYCGRG 2 cut(s) 140, 283
AoxI GGCC 3 cut(s) 36, 160, 330
ApeKI GCWGC 2 cut(s) 101, 174
Asp700I GAANNNNTTC 1 cut(s) 189
AspLEI GCGC 1 cut(s) 320
AsuHPI GGTGA 3 cut(s) 44, 185, 430
AvaI CYCGRG 2 cut(s) 140, 283
BaeGI GKGCMC 1 cut(s) 290
BanI GGYRCC 2 cut(s) 287, 488
BbrPI CACGTG 1 cut(s) 346
BbvI GCAGC 2 cut(s) 88, 186
BcoDI GTCTC 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 493
BisI GCNGC 3 cut(s) 99, 102, 175
BlsI GCNGC 3 cut(s) 100, 103, 176
BmeT110I CYCGRG 2 cut(s) 140, 283
BmgBI CACGTC 1 cut(s) 353
BmiI GGNNCC 2 cut(s) 289, 490
BsaAI YACGTR 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 185
BsaHI GRCGYC 1 cut(s) 16
BsaJI CCNNGG 3 cut(s) 33, 157, 163
BsaXI ACNNNNNCTCC 4 cut(s) 138, 168, 190, 220
Bsc4I CCNNNNNNNGG 1 cut(s) 452
Bse8I GATNNNNATC 1 cut(s) 185
BseDI CCNNGG 3 cut(s) 33, 157, 163
BseJI GATNNNNATC 1 cut(s) 185
BseLI CCNNNNNNNGG 1 cut(s) 452
BseMII CTCAG 1 cut(s) 318
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 2 cut(s) 88, 186
Bsh1236I CGCG 1 cut(s) 320
BshFI GGCC 3 cut(s) 38, 162, 332
BshNI GGYRCC 2 cut(s) 287, 488
BsiHKCI CYCGRG 2 cut(s) 140, 283
BslI CCNNNNNNNGG 1 cut(s) 452
BsmAI GTCTC 1 cut(s) 137
BsnI GGCC 3 cut(s) 38, 162, 332
BsoBI CYCGRG 2 cut(s) 140, 283
Bsp1286I GDGCHC 1 cut(s) 290
Bsp143I GATC 4 cut(s) 49, 261, 441, 465
BspACI CCGC 5 cut(s) 55, 99, 117, 196, 220
BspANI GGCC 3 cut(s) 38, 162, 332
BspCNI CTCAG 1 cut(s) 319
BspFNI CGCG 1 cut(s) 320
BspLI GGNNCC 2 cut(s) 289, 490
BspPI GGATC 2 cut(s) 256, 436
BspT107I GGYRCC 2 cut(s) 287, 488
BssECI CCNNGG 3 cut(s) 33, 157, 163
BssMI GATC 4 cut(s) 49, 261, 441, 465
BssNI GRCGYC 1 cut(s) 16
BssT1I CCWWGG 2 cut(s) 33, 163
Bst4CI ACNGT 2 cut(s) 425, 488
Bst6I CTCTTC 1 cut(s) 118
BstACI GRCGYC 1 cut(s) 16
BstBAI YACGTR 1 cut(s) 346
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 1 cut(s) 327
BstFNI CGCG 1 cut(s) 320
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 4 cut(s) 52, 264, 444, 468
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 4 cut(s) 49, 261, 441, 465
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNSI RCATGY 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 493
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 88, 186
BstX2I RGATCY 2 cut(s) 261, 441
BstXI CCANNNNNNTGG 2 cut(s) 170, 415
BstYI RGATCY 2 cut(s) 261, 441
BsuRI GGCC 3 cut(s) 38, 162, 332
BtrI CACGTC 1 cut(s) 353
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 1 cut(s) 339
CfoI GCGC 1 cut(s) 320
Csp6I GTAC 1 cut(s) 510
CviAII CATG 3 cut(s) 58, 277, 430
CviJI RGCY 8 cut(s) 5, 38, 98, 162, 230, 244, 332, 337
CviKI_1 RGCY 8 cut(s) 5, 38, 98, 162, 230, 244, 332, 337
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 1 cut(s) 327
DpnI GATC 4 cut(s) 51, 263, 443, 467
DpnII GATC 4 cut(s) 49, 261, 441, 465
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 33, 163
Eco32I GATATC 1 cut(s) 256
Eco72I CACGTG 1 cut(s) 346
Eco88I CYCGRG 2 cut(s) 140, 283
EcoRV GATATC 1 cut(s) 256
EcoT14I CCWWGG 2 cut(s) 33, 163
ErhI CCWWGG 2 cut(s) 33, 163
FaeI CATG 3 cut(s) 61, 280, 433
FaiI YATR 7 cut(s) 59, 95, 269, 278, 308, 431, 458
FatI CATG 3 cut(s) 57, 276, 429
Fnu4HI GCNGC 3 cut(s) 99, 102, 175
Fsp4HI GCNGC 3 cut(s) 99, 102, 175
GlaI GCGC 1 cut(s) 319
GluI GCNGC 3 cut(s) 99, 102, 175
HaeIII GGCC 3 cut(s) 38, 162, 332
HhaI GCGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 16
Hin1II CATG 3 cut(s) 61, 280, 433
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 2 cut(s) 203, 281
HphI GGTGA 3 cut(s) 44, 185, 430
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 2 cut(s) 49, 328
Hpy188III TCNNGA 3 cut(s) 86, 142, 259
Hpy8I GTNNAC 1 cut(s) 421
Hpy99I CGWCG 3 cut(s) 18, 21, 509
HpyCH4III ACNGT 2 cut(s) 425, 488
HpyCH4IV ACGT 3 cut(s) 16, 345, 352
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 1 cut(s) 327
HpySE526I ACGT 3 cut(s) 16, 345, 352
Hsp92I GRCGYC 1 cut(s) 16
Hsp92II CATG 3 cut(s) 61, 280, 433
HspAI GCGC 1 cut(s) 318
Kzo9I GATC 4 cut(s) 49, 261, 441, 465
LpnPI CCDG 3 cut(s) 99, 383, 505
Lsp1109I GCAGC 2 cut(s) 88, 186
MaeII ACGT 3 cut(s) 16, 345, 352
MaeIII GTNAC 1 cut(s) 425
MalI GATC 4 cut(s) 51, 263, 443, 467
MboI GATC 4 cut(s) 49, 261, 441, 465
MboII GAAGA 2 cut(s) 105, 451
MflI RGATCY 2 cut(s) 261, 441
MhlI GDGCHC 1 cut(s) 290
MlyI GAGTC 2 cut(s) 197, 275
MnlI CCTC 7 cut(s) 49, 86, 93, 167, 302, 322, 490
MroXI GAANNNNTTC 1 cut(s) 189
MseI TTAA 1 cut(s) 476
MslI CAYNNNNRTG 1 cut(s) 434
MspA1I CMGCKG 2 cut(s) 101, 222
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 1 cut(s) 338
NdeII GATC 4 cut(s) 49, 261, 441, 465
NlaIII CATG 3 cut(s) 61, 280, 433
NlaIV GGNNCC 2 cut(s) 289, 490
NmeAIII GCCGAG 1 cut(s) 138
NspI RCATGY 1 cut(s) 61
PaeR7I CTCGAG 1 cut(s) 140
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 147
PdmI GAANNNNTTC 1 cut(s) 189
PkrI GCNGC 3 cut(s) 100, 103, 176
PleI GAGTC 2 cut(s) 197, 275
PmaCI CACGTG 1 cut(s) 346
PmlI CACGTG 1 cut(s) 346
PpsI GAGTC 2 cut(s) 197, 275
Ppu21I YACGTR 1 cut(s) 346
PspCI CACGTG 1 cut(s) 346
PspN4I GGNNCC 2 cut(s) 289, 490
PsuI RGATCY 2 cut(s) 261, 441
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
RseI CAYNNNNRTG 1 cut(s) 434
SaqAI TTAA 1 cut(s) 476
SatI GCNGC 3 cut(s) 99, 102, 175
Sau3AI GATC 4 cut(s) 49, 261, 441, 465
SchI GAGTC 2 cut(s) 197, 275
SduI GDGCHC 1 cut(s) 290
SetI ASST 7 cut(s) 19, 169, 246, 339, 348, 355, 482
SfcI CTRYAG 1 cut(s) 493
Sfr274I CTCGAG 1 cut(s) 140
SlaI CTCGAG 1 cut(s) 140
SmiMI CAYNNNNRTG 1 cut(s) 434
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
SsiI CCGC 5 cut(s) 55, 99, 117, 196, 220
StyI CCWWGG 2 cut(s) 33, 163
TaaI ACNGT 2 cut(s) 425, 488
TaiI ACGT 3 cut(s) 19, 348, 355
TaqI TCGA 4 cut(s) 13, 141, 150, 294
TauI GCSGC 1 cut(s) 101
Tru1I TTAA 1 cut(s) 476
Tru9I TTAA 1 cut(s) 476
TscAI CASTG 1 cut(s) 205
TseI GCWGC 2 cut(s) 101, 174
TspDTI ATGAA 1 cut(s) 265
TspRI CASTG 1 cut(s) 205
XceI RCATGY 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 189
ZraI GACGTC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.