RchiOBHm_Chr7g0177961

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001823
Physical Location & Seq
Forward (+)
767052 .. 768413
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15857

Sequence Viewer

Length: 987 bp
ATGGCTCTCATCCGGGAACGCCGCCAGCTCAACCTCAGCCTCCCCTTGCCGGAACCCTCCGAGCGTCGCCCACGTTTCACTCTACCTCTCCCTCCCACCGCCGCCGTGGCAACCACCACAAACTGCTCCTCCGCCGCCGCCGCCTCCTTCTCCGCCTCGGACCTCGAAAGACTAGAGGTACTAGGCCACGGTAACGGCGGCACAGTCTACAAAGTCCGCCACAAGCGCACTGGAGCTAACTACGCCCTAAAAATAGTGAAAGGCGACTCCGACCCCACCGTCCGCCGCCAGCTCTACCGCGAAATAGAAATCCTCCGCCGCACGGACTCCCCCCACGTGGTTCACTGCAACGCCATCATCGAGAAGCCCACCGGAGACATCTGGATTCTCATGGAGTACATGGACTCGGGAACATTAGAGACCTCGCTCAAAGAAAACGGCACGTTCTCCGAGGCCAAGGTCGCTCGTGTAGCTCGTCAAGTCCTCAACGGTCTCAATTACCTCCACACTCAGAAGATCATCCACCGTGACATCAAGCCAGCCAATCTGTTAGTCAACAGCAACATGGAAGTCAAGATTGCCGACTTCGGCGTCAGCAAAATGATGTGCCGAACTCTCGATAACTGTAACTCATACGTTGGGACTTGTGCTTACATGAGCCCGGAGAGATTTGACCCCGACACTTACGGTGGAAACTACAATGGGTACGCCAGTGATATATGGAGCTTGGGTCTAACCCTCATGGAGCTTTACATGGGCCACTTTCCGTTCTTGCCTCCGGGTCAGAGACCCGACTGGGCGACCCTTATGTGCGCCATATGTTTCGGGGAGCCACCGGCCTTGCCGGAGGGGGTGTCGGAGGAGTTCAGGAGTTTTATGGAGTGTTGCTTGCAAAAGGAGTCGGGGAAGAGGTGGACGGCGGCTCAGCTTTTGACGCACCCGTTTGTTTGTAAAGATCCGAAAGCCATTCGGTACCTGAAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

328

Amino Acids

36.78

Weight (kDa)

8.27

Isoelectric Point (pI)

50.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 56 - 316 5.5e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 56 - 311 4.6e-41 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 119 - 202 3.3e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 278, 590
Acc65I GGTACC 1 cut(s) 972
AccB1I GGYRCC 1 cut(s) 972
AccI GTMKAC 1 cut(s) 207
AccII CGCG 1 cut(s) 300
AclWI GGATC 1 cut(s) 950
AcvI CACGTG 1 cut(s) 337
AcyI GRCGYC 1 cut(s) 591
AfaI GTAC 4 cut(s) 180, 398, 707, 974
AfiI CCNNNNNNNGG 3 cut(s) 49, 322, 337
AluBI AGCT 7 cut(s) 28, 236, 292, 473, 726, 748, 928
AluI AGCT 7 cut(s) 28, 236, 292, 473, 726, 748, 928
Alw26I GTCTC 4 cut(s) 369, 413, 497, 781
AlwI GGATC 1 cut(s) 950
Ama87I CYCGRG 1 cut(s) 406
AoxI GGCC 4 cut(s) 184, 453, 757, 837
Asp718I GGTACC 1 cut(s) 972
AspLEI GCGC 2 cut(s) 228, 815
AspS9I GGNCC 2 cut(s) 160, 757
AsuC2I CCSGG 3 cut(s) 14, 662, 780
AvaI CYCGRG 1 cut(s) 406
AvaII GGWCC 1 cut(s) 160
BaeI ACNNNNGTAYC 2 cut(s) 697, 730
BanI GGYRCC 1 cut(s) 972
BanII GRGCYC 1 cut(s) 662
BauI CACGAG 1 cut(s) 465
BbrPI CACGTG 1 cut(s) 337
BbvCI CCTCAGC 1 cut(s) 35
BccI CCATC 1 cut(s) 362
BceAI ACGGC 4 cut(s) 89, 211, 454, 933
BcnI CCSGG 3 cut(s) 14, 662, 780
BcoDI GTCTC 4 cut(s) 369, 413, 497, 781
BfaI CTAG 2 cut(s) 173, 182
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 9 cut(s) 22, 102, 135, 138, 141, 199, 286, 319, 921
BlpI GCTNAGC 1 cut(s) 924
BlsI GCNGC 9 cut(s) 23, 103, 136, 139, 142, 200, 287, 320, 922
Bme1390I CCNGG 3 cut(s) 14, 662, 780
Bme18I GGWCC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 406
BmgT120I GGNCC 2 cut(s) 160, 757
BmiI GGNNCC 3 cut(s) 54, 831, 974
BmrFI CCNGG 3 cut(s) 14, 662, 780
BmrI ACTGGG 1 cut(s) 805
BmuI ACTGGG 1 cut(s) 805
BpmI CTGGAG 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 35
Bpu1102I GCTNAGC 1 cut(s) 924
BpuMI CCSGG 3 cut(s) 14, 662, 780
BsaAI YACGTR 1 cut(s) 337
BsaHI GRCGYC 1 cut(s) 591
BsaI GGTCTC 3 cut(s) 413, 497, 781
BsaJI CCNNGG 5 cut(s) 105, 156, 187, 450, 456
BsaWI WCCGGW 1 cut(s) 371
BsaXI ACNNNNNCTCC 6 cut(s) 113, 143, 715, 745, 839, 869
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 322, 337
Bse118I RCCGGY 1 cut(s) 835
Bse1I ACTGG 3 cut(s) 235, 711, 800
BseDI CCNNGG 5 cut(s) 105, 156, 187, 450, 456
BseGI GGATG 2 cut(s) 9, 519
BseLI CCNNNNNNNGG 3 cut(s) 49, 322, 337
BseMII CTCAG 3 cut(s) 49, 524, 938
BseNI ACTGG 3 cut(s) 235, 711, 800
BseRI GAGGAG 2 cut(s) 118, 875
Bsh1236I CGCG 1 cut(s) 300
BshFI GGCC 4 cut(s) 186, 455, 759, 839
BshNI GGYRCC 1 cut(s) 972
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 7 cut(s) 13, 50, 372, 662, 779, 836, 845
BslFI GGGAC 1 cut(s) 655
BslI CCNNNNNNNGG 3 cut(s) 49, 322, 337
BsmAI GTCTC 4 cut(s) 369, 413, 497, 781
BsmFI GGGAC 1 cut(s) 655
BsnI GGCC 4 cut(s) 186, 455, 759, 839
Bso31I GGTCTC 3 cut(s) 413, 497, 781
BsoBI CYCGRG 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 662
Bsp143I GATC 2 cut(s) 516, 955
Bsp1720I GCTNAGC 1 cut(s) 924
BspANI GGCC 4 cut(s) 186, 455, 759, 839
BspCNI CTCAG 3 cut(s) 48, 523, 937
BspFNI CGCG 1 cut(s) 300
BspLI GGNNCC 3 cut(s) 54, 831, 974
BspPI GGATC 1 cut(s) 950
BspT107I GGYRCC 1 cut(s) 972
BspTNI GGTCTC 3 cut(s) 413, 497, 781
BsrFI RCCGGY 1 cut(s) 835
BsrI ACTGG 3 cut(s) 235, 711, 800
BssAI RCCGGY 1 cut(s) 835
BssECI CCNNGG 5 cut(s) 105, 156, 187, 450, 456
BssMI GATC 2 cut(s) 516, 955
BssNI GRCGYC 1 cut(s) 591
BssSI CACGAG 1 cut(s) 465
BssT1I CCWWGG 1 cut(s) 456
Bst2BI CACGAG 1 cut(s) 465
Bst4CI ACNGT 7 cut(s) 191, 205, 280, 491, 527, 626, 689
Bst6I CTCTTC 1 cut(s) 902
BstACI GRCGYC 1 cut(s) 591
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 4 cut(s) 26, 290, 540, 890
BstDEI CTNAG 3 cut(s) 35, 510, 924
BstDSI CCRYGG 2 cut(s) 105, 187
BstF5I GGATG 2 cut(s) 9, 519
BstFNI CGCG 1 cut(s) 300
BstHHI GCGC 2 cut(s) 228, 815
BstKTI GATC 2 cut(s) 519, 958
BstMAI GTCTC 4 cut(s) 369, 413, 497, 781
BstMBI GATC 2 cut(s) 516, 955
BstMWI GCNNNNNNNGC 7 cut(s) 107, 140, 225, 242, 461, 470, 934
BstSCI CCNGG 3 cut(s) 12, 660, 778
BstUI CGCG 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 955
BstYI RGATCY 1 cut(s) 955
BsuRI GGCC 4 cut(s) 186, 455, 759, 839
BtgI CCRYGG 2 cut(s) 105, 187
BtsCI GGATG 2 cut(s) 9, 519
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 3 cut(s) 228, 343, 718
Cac8I GCNNGC 4 cut(s) 26, 290, 540, 890
CfoI GCGC 2 cut(s) 228, 815
Cfr10I RCCGGY 1 cut(s) 835
Cfr13I GGNCC 2 cut(s) 160, 757
CseI GACGC 3 cut(s) 53, 580, 943
Csp6I GTAC 4 cut(s) 179, 397, 706, 973
CspCI CAANNNNNGTGG 2 cut(s) 822, 857
CviAII CATG 6 cut(s) 391, 400, 565, 655, 742, 754
CviQI GTAC 4 cut(s) 179, 397, 706, 973
DdeI CTNAG 3 cut(s) 35, 510, 924
DpnI GATC 2 cut(s) 518, 957
DpnII GATC 2 cut(s) 516, 955
DrdI GACNNNNNNGTC 2 cut(s) 278, 590
DseDI GACNNNNNNGTC 2 cut(s) 278, 590
Eam1104I CTCTTC 1 cut(s) 902
EarI CTCTTC 1 cut(s) 902
EciI GGCGGA 5 cut(s) 121, 142, 206, 272, 305
Eco130I CCWWGG 1 cut(s) 456
Eco24I GRGCYC 1 cut(s) 662
Eco31I GGTCTC 3 cut(s) 413, 497, 781
Eco47I GGWCC 1 cut(s) 160
Eco72I CACGTG 1 cut(s) 337
Eco88I CYCGRG 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 456
EcoT38I GRGCYC 1 cut(s) 662
ErhI CCWWGG 1 cut(s) 456
FaeI CATG 6 cut(s) 394, 403, 568, 658, 745, 757
FaqI GGGAC 1 cut(s) 655
FatI CATG 6 cut(s) 390, 399, 564, 654, 741, 753
FauNDI CATATG 1 cut(s) 818
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 9 cut(s) 22, 102, 135, 138, 141, 199, 286, 319, 921
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 1 cut(s) 662
Fsp4HI GCNGC 9 cut(s) 22, 102, 135, 138, 141, 199, 286, 319, 921
FspBI CTAG 2 cut(s) 173, 182
GlaI GCGC 2 cut(s) 227, 814
GluI GCNGC 9 cut(s) 22, 102, 135, 138, 141, 199, 286, 319, 921
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 4 cut(s) 186, 455, 759, 839
HapII CCGG 7 cut(s) 13, 50, 372, 662, 779, 836, 845
HgaI GACGC 3 cut(s) 53, 580, 943
HhaI GCGC 2 cut(s) 228, 815
Hin1I GRCGYC 1 cut(s) 591
Hin1II CATG 6 cut(s) 394, 403, 568, 658, 745, 757
Hin6I GCGC 2 cut(s) 226, 813
HinP1I GCGC 2 cut(s) 226, 813
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 5 cut(s) 266, 326, 385, 404, 899
HpaII CCGG 7 cut(s) 13, 50, 372, 662, 779, 836, 845
Hpy166II GTNNAC 4 cut(s) 208, 343, 556, 915
Hpy188I TCNGA 8 cut(s) 61, 160, 271, 451, 513, 786, 859, 960
Hpy188III TCNNGA 6 cut(s) 361, 382, 408, 574, 617, 868
Hpy8I GTNNAC 4 cut(s) 208, 343, 556, 915
Hpy99I CGWCG 1 cut(s) 69
HpyAV CCTTC 2 cut(s) 157, 973
HpyCH4III ACNGT 7 cut(s) 191, 205, 280, 491, 527, 626, 689
HpyCH4IV ACGT 4 cut(s) 73, 336, 443, 636
HpyCH4V TGCA 2 cut(s) 348, 892
HpyF10VI GCNNNNNNNGC 7 cut(s) 107, 140, 225, 242, 461, 470, 934
HpyF3I CTNAG 3 cut(s) 35, 510, 924
HpySE526I ACGT 4 cut(s) 73, 336, 443, 636
Hsp92I GRCGYC 1 cut(s) 591
Hsp92II CATG 6 cut(s) 394, 403, 568, 658, 745, 757
HspAI GCGC 2 cut(s) 226, 813
KpnI GGTACC 1 cut(s) 976
Kzo9I GATC 2 cut(s) 516, 955
LmnI GCTCC 5 cut(s) 131, 233, 723, 745, 829
MaeI CTAG 2 cut(s) 173, 182
MaeII ACGT 4 cut(s) 73, 336, 443, 636
MaeIII GTNAC 3 cut(s) 191, 527, 626
MalI GATC 2 cut(s) 518, 957
MboI GATC 2 cut(s) 516, 955
MboII GAAGA 2 cut(s) 526, 919
MflI RGATCY 1 cut(s) 955
MhlI GDGCHC 1 cut(s) 662
MluCI AATT 1 cut(s) 496
MlyI GAGTC 4 cut(s) 260, 320, 398, 908
MmeI TCCRAC 2 cut(s) 294, 837
MseI TTAA 1 cut(s) 985
MspI CCGG 7 cut(s) 13, 50, 372, 662, 779, 836, 845
MspR9I CCNGG 3 cut(s) 14, 662, 780
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 7 cut(s) 107, 140, 225, 242, 461, 470, 934
NciI CCSGG 3 cut(s) 14, 662, 780
NdeI CATATG 1 cut(s) 818
NdeII GATC 2 cut(s) 516, 955
NlaIII CATG 6 cut(s) 394, 403, 568, 658, 745, 757
NlaIV GGNNCC 3 cut(s) 54, 831, 974
NmuCI GTSAC 1 cut(s) 527
PcsI WCGNNNNNNNCGW 2 cut(s) 357, 472
PfeI GAWTC 1 cut(s) 385
PfoI TCCNGGA 1 cut(s) 12
PkrI GCNGC 9 cut(s) 23, 103, 136, 139, 142, 200, 287, 320, 922
PleI GAGTC 4 cut(s) 260, 320, 398, 907
PmaCI CACGTG 1 cut(s) 337
PmlI CACGTG 1 cut(s) 337
PpsI GAGTC 4 cut(s) 260, 320, 398, 907
Ppu21I YACGTR 1 cut(s) 337
PspCI CACGTG 1 cut(s) 337
PspN4I GGNNCC 3 cut(s) 54, 831, 974
PspPI GGNCC 2 cut(s) 160, 757
PsuI RGATCY 1 cut(s) 955
RsaI GTAC 4 cut(s) 180, 398, 707, 974
RsaNI GTAC 4 cut(s) 179, 397, 706, 973
SaqAI TTAA 1 cut(s) 985
SatI GCNGC 9 cut(s) 22, 102, 135, 138, 141, 199, 286, 319, 921
Sau3AI GATC 2 cut(s) 516, 955
Sau96I GGNCC 2 cut(s) 160, 757
SchI GAGTC 4 cut(s) 260, 320, 398, 908
ScrFI CCNGG 3 cut(s) 14, 662, 780
SduI GDGCHC 1 cut(s) 662
SinI GGWCC 1 cut(s) 160
Sse9I AATT 1 cut(s) 496
SspMI CTAG 2 cut(s) 173, 182
StyD4I CCNGG 3 cut(s) 12, 660, 778
StyI CCWWGG 1 cut(s) 456
TaaI ACNGT 7 cut(s) 191, 205, 280, 491, 527, 626, 689
TaiI ACGT 4 cut(s) 76, 339, 446, 639
TaqI TCGA 3 cut(s) 165, 360, 618
TasI AATT 1 cut(s) 496
TatI WGTACW 1 cut(s) 396
TauI GCSGC 9 cut(s) 24, 104, 137, 140, 143, 201, 288, 321, 923
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 1 cut(s) 985
Tru9I TTAA 1 cut(s) 985
TscAI CASTG 3 cut(s) 235, 350, 718
TseFI GTSAC 1 cut(s) 527
Tsp45I GTSAC 1 cut(s) 527
TspGWI ACGGA 2 cut(s) 338, 756
TspRI CASTG 3 cut(s) 235, 350, 718
VpaK11BI GGWCC 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 2 cut(s) 103, 227
XmiI GTMKAC 1 cut(s) 207
XspI CTAG 2 cut(s) 173, 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.