Rmu_sc0002154.1_g000010

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002154.1
OG0001823
Physical Location & Seq
Forward (+)
43889 .. 47976
4088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002154.1_g000010.1.cds

Sequence Viewer

Length: 1965 bp
atggctctcatccgggaacgccgccagctcaacctcagcctccccttgccggaaccctccgagcgtcgcccacgtttcactctacctctccctcccaccgccgctgtggcaaccaccaccaactgctcctccgccttctccgcctcggacctcgaaagactagaggtactaggccacggtaacggcggcacagttgaaaggcgactccgaccccaccgtccgccgcctgtggcaaccaccaccaactgctcctccgccttctccgcctcggacctcgaaagactagaggtactaggccacggtaacggcggcacagtctacaaagtccgccacaagcgcactggagctaactacgccctaaaaatagtgaaaggcgactccgaccccaccgtccgccgccagctctaccgcgaaatagaaatcctccgccgcacggactccccccacgtggttcactgcaacgccatcatcgagaagcccaccggagacatctggattctcatggagtacatggactcgggaacattagagacctcgctcaaagaaaacggcacgttctccgaggccaaggtcgctcgtgtagctcgtcaagtcctcaacggtctcaattacctccacactcagaagatcatccaccgtgacatcaagccggccaatctgttagtcaacagcaacatggaagtcaagattgccgacttcggcgtcagcaaaatgatgtgccgaactctcgataactgtaactcatacgttgggacttgtgcttacatgagcccggagagatttgaccctgacacttacggtggaaactacaatggatacgccagtgatatatggagcttgggtctaaccctcatggagctttacatgggccactttccgttcttgcctccgggtcagagacccgactgggcgacccttatgtgcgccatttgtttcggggagccaccggccttgccggagggggtgtcggaggagttcaggagttttatggagtgttgcttgcaaaaggagtcggggaagaggtggacggcggctcagcttttgacgcacccgtttgtttgtaaagatccgaaagccattcgacccaattcaccaccacctagtccgatgcttcagtcgtcgaccccaaaccaacaacaactatccaacttctgctgctcgtcgaggcttggtcgtcacaactcccccaaaccgactgccgacatccatttcaatctccttcgaccccaaaccagtaaccaagatccaaatcccaacccctctaggtctgcaagatccgaaaacccacccctcagcgcccctgaactctccttcctcctcccatctaccatcgtcgcctatggccagagctcgtcctacgacaacgggagtgacagtataaagtttgaggcctcctttgggctgcttgcagggaggcccccttcacctccatcagatctctcagattctcgatcttcggcatcgcgggctcgttggttgacagtgatatgtctcaagttagggttgcgaccaccgcatggtagatatgggttcgagatcgggcagcgcctaccttcttaccgtgattggaggagctttgatggcagtactgggcaacttgcggcggtgcaaagtgttgttcgggcctcgttttggtggatgggattgctggcggcgccggtgatttggccagctttggtattgacaccgacgtcgatggcttggatgctcggattcttaatccagagaggctgttgccgtgctctctggtggctacgttgggtggagacgaagctgggggcgtctgaaaggcgacgaccatatctaggtgcccggcggtgggtgtccaaatcagattgggtcttcaggaggttgggctggtgtggggtccacttcactactctgaagggccctatttctctatgggcttggatttgggaccctggagattttgtgcatgatgtttggtttgattacactctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

654

Amino Acids

73.28

Weight (kDa)

9.16

Isoelectric Point (pI)

59.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 216, 389, 701, 1690
AatII GACGTC 1 cut(s) 1694
AccB1I GGYRCC 2 cut(s) 1654, 1808
AccB7I CCANNNNNTGG 1 cut(s) 1517
AccI GTMKAC 2 cut(s) 318, 1121
AccII CGCG 2 cut(s) 411, 1465
AclWI GGATC 3 cut(s) 1061, 1238, 1269
AcoI YGGCCR 3 cut(s) 651, 1342, 1667
AcuI CTGAAG 3 cut(s) 1097, 1828, 1904
AcvI CACGTG 1 cut(s) 448
AcyI GRCGYC 4 cut(s) 702, 1655, 1691, 1781
AfaI GTAC 4 cut(s) 168, 291, 509, 1588
AfiI CCNNNNNNNGG 9 cut(s) 49, 433, 448, 1398, 1517, 1632, 1805, 1818, 1819
AgsI TTSAA 2 cut(s) 197, 1213
AjnI CCWGG 1 cut(s) 1921
AloI GAACNNNNNNTCC 2 cut(s) 1296, 1328
Alw21I GWGCWC 2 cut(s) 1352, 1744
Alw26I GTCTC 6 cut(s) 480, 524, 608, 892, 1496, 1760
AlwI GGATC 3 cut(s) 1061, 1238, 1269
Ama87I CYCGRG 1 cut(s) 517
ApaI GGGCCC 1 cut(s) 1892
ApeKI GCWGC 3 cut(s) 1155, 1402, 1543
AspLEI GCGC 5 cut(s) 339, 926, 1298, 1548, 1657
AspS9I GGNCC 9 cut(s) 148, 271, 868, 1416, 1623, 1867, 1888, 1889, 1918
AsuC2I CCSGG 4 cut(s) 14, 773, 891, 1813
AsuHPI GGTGA 3 cut(s) 1083, 1416, 1672
AvaI CYCGRG 1 cut(s) 517
AvaII GGWCC 4 cut(s) 148, 271, 1867, 1918
BaeGI GKGCMC 2 cut(s) 1813, 1892
BaeI ACNNNNGTAYC 2 cut(s) 808, 841
BalI TGGCCA 2 cut(s) 1344, 1669
BanI GGYRCC 2 cut(s) 1654, 1808
BanII GRGCYC 4 cut(s) 773, 1352, 1471, 1892
BauI CACGAG 1 cut(s) 576
BbrPI CACGTG 1 cut(s) 448
BbsI GAAGAC 1 cut(s) 1834
Bbv12I GWGCWC 2 cut(s) 1352, 1744
BbvCI CCTCAGC 2 cut(s) 35, 1292
BbvI GCAGC 3 cut(s) 1142, 1389, 1555
BccI CCATC 7 cut(s) 473, 1330, 1337, 1438, 1574, 1633, 1690
BceAI ACGGC 5 cut(s) 199, 322, 565, 1044, 1722
BciT130I CCWGG 1 cut(s) 1923
BciVI GTATCC 1 cut(s) 809
BcnI CCSGG 4 cut(s) 14, 773, 891, 1813
BcoDI GTCTC 6 cut(s) 480, 524, 608, 892, 1496, 1760
BfaI CTAG 7 cut(s) 161, 170, 284, 293, 1101, 1263, 1805
BfoI RGCGCY 3 cut(s) 1299, 1549, 1658
BfuI GTATCC 1 cut(s) 809
BglI GCCNNNNNGGC 1 cut(s) 107
BglII AGATCT 1 cut(s) 1435
BlpI GCTNAGC 1 cut(s) 1035
BmcAI AGTACT 1 cut(s) 1588
Bme1390I CCNGG 5 cut(s) 14, 773, 891, 1813, 1923
Bme18I GGWCC 4 cut(s) 148, 271, 1867, 1918
BmeT110I CYCGRG 1 cut(s) 517
BmgT120I GGNCC 9 cut(s) 148, 271, 868, 1416, 1623, 1867, 1888, 1889, 1918
BmiI GGNNCC 9 cut(s) 54, 942, 1418, 1656, 1810, 1868, 1890, 1919, 1920
BmrFI CCNGG 5 cut(s) 14, 773, 891, 1813, 1923
BmrI ACTGGG 2 cut(s) 916, 1599
BmsI GCATC 3 cut(s) 1098, 1469, 1695
BmuI ACTGGG 2 cut(s) 916, 1599
BpiI GAAGAC 1 cut(s) 1834
BpmI CTGGAG 2 cut(s) 363, 1944
Bpu10I CCTNAGC 2 cut(s) 35, 1292
Bpu1102I GCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 1 cut(s) 1478
BpuMI CCSGG 4 cut(s) 14, 773, 891, 1813
BsaAI YACGTR 1 cut(s) 448
BsaBI GATNNNNATC 1 cut(s) 1248
BsaHI GRCGYC 4 cut(s) 702, 1655, 1691, 1781
BsaI GGTCTC 3 cut(s) 524, 608, 892
BsaJI CCNNGG 7 cut(s) 144, 175, 267, 298, 561, 567, 1921
BsaWI WCCGGW 1 cut(s) 482
Bsc4I CCNNNNNNNGG 9 cut(s) 49, 433, 448, 1398, 1517, 1632, 1805, 1818, 1819
Bse118I RCCGGY 3 cut(s) 649, 946, 1657
Bse1I ACTGG 5 cut(s) 346, 822, 911, 1233, 1594
Bse8I GATNNNNATC 1 cut(s) 1248
BseBI CCWGG 1 cut(s) 1923
BseDI CCNNGG 7 cut(s) 144, 175, 267, 298, 561, 567, 1921
BseGI GGATG 5 cut(s) 9, 630, 1203, 1644, 1710
BseJI GATNNNNATC 1 cut(s) 1248
BseLI CCNNNNNNNGG 9 cut(s) 49, 433, 448, 1398, 1517, 1632, 1805, 1818, 1819
BseMII CTCAG 5 cut(s) 49, 635, 1049, 1306, 1455
BseNI ACTGG 5 cut(s) 346, 822, 911, 1233, 1594
BseRI GAGGAG 5 cut(s) 118, 241, 986, 1307, 1585
BseSI GKGCMC 2 cut(s) 1813, 1892
BseXI GCAGC 3 cut(s) 1142, 1389, 1555
BseYI CCCAGC 1 cut(s) 1774
Bsh1236I CGCG 2 cut(s) 411, 1465
BshNI GGYRCC 2 cut(s) 1654, 1808
BsiHKAI GWGCWC 2 cut(s) 1352, 1744
BsiHKCI CYCGRG 1 cut(s) 517
BslFI GGGAC 2 cut(s) 766, 1931
BslI CCNNNNNNNGG 9 cut(s) 49, 433, 448, 1398, 1517, 1632, 1805, 1818, 1819
BsmAI GTCTC 6 cut(s) 480, 524, 608, 892, 1496, 1760
BsmBI CGTCTC 1 cut(s) 1760
BsmFI GGGAC 2 cut(s) 766, 1931
Bso31I GGTCTC 3 cut(s) 524, 608, 892
BsoBI CYCGRG 1 cut(s) 517
Bsp120I GGGCCC 1 cut(s) 1888
Bsp1286I GDGCHC 6 cut(s) 773, 1352, 1471, 1744, 1813, 1892
Bsp143I GATC 7 cut(s) 627, 1066, 1243, 1274, 1435, 1451, 1536
Bsp1720I GCTNAGC 1 cut(s) 1035
BspCNI CTCAG 5 cut(s) 48, 634, 1048, 1305, 1454
BspFNI CGCG 2 cut(s) 411, 1465
BspLI GGNNCC 9 cut(s) 54, 942, 1418, 1656, 1810, 1868, 1890, 1919, 1920
BspPI GGATC 3 cut(s) 1061, 1238, 1269
BspT107I GGYRCC 2 cut(s) 1654, 1808
BspTNI GGTCTC 3 cut(s) 524, 608, 892
BsrFI RCCGGY 3 cut(s) 649, 946, 1657
BsrI ACTGG 5 cut(s) 346, 822, 911, 1233, 1594
BssAI RCCGGY 3 cut(s) 649, 946, 1657
BssECI CCNNGG 7 cut(s) 144, 175, 267, 298, 561, 567, 1921
BssMI GATC 7 cut(s) 627, 1066, 1243, 1274, 1435, 1451, 1536
BssNI GRCGYC 4 cut(s) 702, 1655, 1691, 1781
BssSI CACGAG 1 cut(s) 576
BssT1I CCWWGG 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 576
Bst2UI CCWGG 1 cut(s) 1923
Bst6I CTCTTC 1 cut(s) 1013
BstACI GRCGYC 4 cut(s) 702, 1655, 1691, 1781
BstBAI YACGTR 1 cut(s) 448
BstC8I GCNNGC 8 cut(s) 26, 401, 651, 1001, 1407, 1467, 1650, 1671
BstDEI CTNAG 5 cut(s) 35, 621, 1035, 1292, 1441
BstDSI CCRYGG 2 cut(s) 175, 298
BstF5I GGATG 5 cut(s) 9, 630, 1203, 1644, 1710
BstFNI CGCG 2 cut(s) 411, 1465
BstH2I RGCGCY 3 cut(s) 1299, 1549, 1658
BstHHI GCGC 5 cut(s) 339, 926, 1298, 1548, 1657
BstKTI GATC 7 cut(s) 630, 1069, 1246, 1277, 1438, 1454, 1539
BstMAI GTCTC 6 cut(s) 480, 524, 608, 892, 1496, 1760
BstMBI GATC 7 cut(s) 627, 1066, 1243, 1274, 1435, 1451, 1536
BstNI CCWGG 1 cut(s) 1923
BstSCI CCNGG 5 cut(s) 12, 771, 889, 1811, 1921
BstSLI GKGCMC 2 cut(s) 1813, 1892
BstUI CGCG 2 cut(s) 411, 1465
BstV1I GCAGC 3 cut(s) 1142, 1389, 1555
BstV2I GAAGAC 1 cut(s) 1834
BstX2I RGATCY 4 cut(s) 1066, 1243, 1274, 1435
BstYI RGATCY 4 cut(s) 1066, 1243, 1274, 1435
BsuI GTATCC 1 cut(s) 809
BtgI CCRYGG 2 cut(s) 175, 298
BtgZI GCGATG 1 cut(s) 1446
BtsCI GGATG 5 cut(s) 9, 630, 1203, 1644, 1710
BtsI GCAGTG 1 cut(s) 454
BtsIMutI CAGTG 4 cut(s) 339, 454, 829, 1488
Cac8I GCNNGC 8 cut(s) 26, 401, 651, 1001, 1407, 1467, 1650, 1671
CfoI GCGC 5 cut(s) 339, 926, 1298, 1548, 1657
Cfr10I RCCGGY 3 cut(s) 649, 946, 1657
Cfr13I GGNCC 9 cut(s) 148, 271, 868, 1416, 1623, 1867, 1888, 1889, 1918
CseI GACGC 4 cut(s) 53, 691, 1054, 1770
Csp6I GTAC 4 cut(s) 167, 290, 508, 1587
CspCI CAANNNNNGTGG 2 cut(s) 933, 968
CviAII CATG 8 cut(s) 502, 511, 676, 766, 853, 865, 1517, 1937
CviQI GTAC 4 cut(s) 167, 290, 508, 1587
DdeI CTNAG 5 cut(s) 35, 621, 1035, 1292, 1441
DinI GGCGCC 1 cut(s) 1656
DpnI GATC 7 cut(s) 629, 1068, 1245, 1276, 1437, 1453, 1538
DpnII GATC 7 cut(s) 627, 1066, 1243, 1274, 1435, 1451, 1536
DrdI GACNNNNNNGTC 4 cut(s) 216, 389, 701, 1690
DseDI GACNNNNNNGTC 4 cut(s) 216, 389, 701, 1690
EaeI YGGCCR 3 cut(s) 651, 1342, 1667
Eam1104I CTCTTC 1 cut(s) 1013
EarI CTCTTC 1 cut(s) 1013
EciI GGCGGA 8 cut(s) 121, 130, 210, 244, 253, 317, 383, 416
Ecl136II GAGCTC 1 cut(s) 1350
Eco130I CCWWGG 1 cut(s) 567
Eco147I AGGCCT 1 cut(s) 1391
Eco24I GRGCYC 4 cut(s) 773, 1352, 1471, 1892
Eco31I GGTCTC 3 cut(s) 524, 608, 892
Eco47I GGWCC 4 cut(s) 148, 271, 1867, 1918
Eco53kI GAGCTC 1 cut(s) 1350
Eco57I CTGAAG 3 cut(s) 1097, 1828, 1904
Eco72I CACGTG 1 cut(s) 448
Eco88I CYCGRG 1 cut(s) 517
EcoICRI GAGCTC 1 cut(s) 1350
EcoO109I RGGNCCY 4 cut(s) 1416, 1888, 1889, 1918
EcoRII CCWGG 1 cut(s) 1921
EcoT14I CCWWGG 1 cut(s) 567
EcoT38I GRGCYC 4 cut(s) 773, 1352, 1471, 1892
EgeI GGCGCC 1 cut(s) 1656
EheI GGCGCC 1 cut(s) 1656
ErhI CCWWGG 1 cut(s) 567
Esp3I CGTCTC 1 cut(s) 1760
FaeI CATG 8 cut(s) 505, 514, 679, 769, 856, 868, 1520, 1940
FaqI GGGAC 2 cut(s) 766, 1931
FatI CATG 8 cut(s) 501, 510, 675, 765, 852, 864, 1516, 1936
FauI CCCGC 1 cut(s) 1458
FblI GTMKAC 2 cut(s) 318, 1121
FokI GGATG 4 cut(s) 617, 1190, 1651, 1717
FriOI GRGCYC 4 cut(s) 773, 1352, 1471, 1892
FspBI CTAG 7 cut(s) 161, 170, 284, 293, 1101, 1263, 1805
GlaI GCGC 5 cut(s) 338, 925, 1297, 1547, 1656
GsaI CCCAGC 1 cut(s) 1778
GsuI CTGGAG 2 cut(s) 363, 1944
HaeII RGCGCY 3 cut(s) 1299, 1549, 1658
HgaI GACGC 4 cut(s) 53, 691, 1054, 1770
HhaI GCGC 5 cut(s) 339, 926, 1298, 1548, 1657
Hin1I GRCGYC 4 cut(s) 702, 1655, 1691, 1781
Hin1II CATG 8 cut(s) 505, 514, 679, 769, 856, 868, 1520, 1940
Hin6I GCGC 5 cut(s) 337, 924, 1296, 1546, 1655
HinP1I GCGC 5 cut(s) 337, 924, 1296, 1546, 1655
HincII GTYRAC 3 cut(s) 667, 1122, 1479
HindII GTYRAC 3 cut(s) 667, 1122, 1479
HinfI GANTC 8 cut(s) 204, 377, 437, 496, 515, 1010, 1445, 1713
HphI GGTGA 3 cut(s) 1083, 1416, 1672
Hpy166II GTNNAC 7 cut(s) 319, 454, 667, 1026, 1122, 1479, 1870
Hpy8I GTNNAC 7 cut(s) 319, 454, 667, 1026, 1122, 1479, 1870
Hpy99I CGWCG 7 cut(s) 69, 1123, 1165, 1337, 1693, 1696, 1797
HpyAV CCTTC 7 cut(s) 145, 268, 1229, 1321, 1431, 1563, 1879
HpyCH4IV ACGT 6 cut(s) 73, 447, 554, 747, 1691, 1756
HpyCH4V TGCA 6 cut(s) 459, 1003, 1271, 1409, 1609, 1936
HpyF3I CTNAG 5 cut(s) 35, 621, 1035, 1292, 1441
HpySE526I ACGT 6 cut(s) 73, 447, 554, 747, 1691, 1756
Hsp92I GRCGYC 4 cut(s) 702, 1655, 1691, 1781
Hsp92II CATG 8 cut(s) 505, 514, 679, 769, 856, 868, 1520, 1940
HspAI GCGC 5 cut(s) 337, 924, 1296, 1546, 1655
KasI GGCGCC 1 cut(s) 1654
KflI GGGWCCC 1 cut(s) 1918
KroI GCCGGC 1 cut(s) 649
KroNI GCCGGC 1 cut(s) 651
Kzo9I GATC 7 cut(s) 627, 1066, 1243, 1274, 1435, 1451, 1536
LmnI GCTCC 7 cut(s) 131, 254, 344, 834, 856, 940, 1572
Lsp1109I GCAGC 3 cut(s) 1142, 1389, 1555
LweI GCATC 3 cut(s) 1098, 1469, 1695
MaeI CTAG 7 cut(s) 161, 170, 284, 293, 1101, 1263, 1805
MaeII ACGT 6 cut(s) 73, 447, 554, 747, 1691, 1756
MaeIII GTNAC 7 cut(s) 179, 302, 638, 737, 1175, 1235, 1370
MalI GATC 7 cut(s) 629, 1068, 1245, 1276, 1437, 1453, 1538
MboI GATC 7 cut(s) 627, 1066, 1243, 1274, 1435, 1451, 1536
MboII GAAGA 4 cut(s) 637, 1030, 1446, 1834
MflI RGATCY 4 cut(s) 1066, 1243, 1274, 1435
MhlI GDGCHC 6 cut(s) 773, 1352, 1471, 1744, 1813, 1892
MlsI TGGCCA 2 cut(s) 1344, 1669
MluCI AATT 2 cut(s) 607, 1087
MluNI TGGCCA 2 cut(s) 1344, 1669
Mly113I GGCGCC 1 cut(s) 1655
MlyI GAGTC 5 cut(s) 198, 371, 431, 509, 1019
MmeI TCCRAC 4 cut(s) 232, 405, 948, 1170
Mox20I TGGCCA 2 cut(s) 1344, 1669
MroNI GCCGGC 1 cut(s) 649
MscI TGGCCA 2 cut(s) 1344, 1669
MseI TTAA 1 cut(s) 1718
Msp20I TGGCCA 2 cut(s) 1344, 1669
MspA1I CMGCKG 1 cut(s) 104
MspR9I CCNGG 5 cut(s) 14, 773, 891, 1813, 1923
MvaI CCWGG 1 cut(s) 1923
MvnI CGCG 2 cut(s) 411, 1465
NaeI GCCGGC 1 cut(s) 651
NarI GGCGCC 1 cut(s) 1655
NciI CCSGG 4 cut(s) 14, 773, 891, 1813
NdeII GATC 7 cut(s) 627, 1066, 1243, 1274, 1435, 1451, 1536
NgoMIV GCCGGC 1 cut(s) 649
NlaIII CATG 8 cut(s) 505, 514, 679, 769, 856, 868, 1520, 1940
NlaIV GGNNCC 9 cut(s) 54, 942, 1418, 1656, 1810, 1868, 1890, 1919, 1920
NmuCI GTSAC 3 cut(s) 638, 1175, 1370
PceI AGGCCT 1 cut(s) 1391
PcsI WCGNNNNNNNCGW 2 cut(s) 468, 583
PdiI GCCGGC 1 cut(s) 651
PfeI GAWTC 3 cut(s) 496, 1445, 1713
PflMI CCANNNNNTGG 1 cut(s) 1517
PfoI TCCNGGA 1 cut(s) 12
PleI GAGTC 5 cut(s) 198, 371, 431, 509, 1018
PluTI GGCGCC 1 cut(s) 1658
PmaCI CACGTG 1 cut(s) 448
PmlI CACGTG 1 cut(s) 448
PpsI GAGTC 5 cut(s) 198, 371, 431, 509, 1018
Ppu21I YACGTR 1 cut(s) 448
PpuMI RGGWCCY 1 cut(s) 1918
Psp124BI GAGCTC 1 cut(s) 1352
Psp5II RGGWCCY 1 cut(s) 1918
Psp6I CCWGG 1 cut(s) 1921
PspCI CACGTG 1 cut(s) 448
PspFI CCCAGC 1 cut(s) 1774
PspGI CCWGG 1 cut(s) 1921
PspN4I GGNNCC 9 cut(s) 54, 942, 1418, 1656, 1810, 1868, 1890, 1919, 1920
PspOMI GGGCCC 1 cut(s) 1888
PspPI GGNCC 9 cut(s) 148, 271, 868, 1416, 1623, 1867, 1888, 1889, 1918
PspPPI RGGWCCY 1 cut(s) 1918
PsuI RGATCY 4 cut(s) 1066, 1243, 1274, 1435
RsaI GTAC 4 cut(s) 168, 291, 509, 1588
RsaNI GTAC 4 cut(s) 167, 290, 508, 1587
SacI GAGCTC 1 cut(s) 1352
SalI GTCGAC 1 cut(s) 1120
SaqAI TTAA 1 cut(s) 1718
Sau3AI GATC 7 cut(s) 627, 1066, 1243, 1274, 1435, 1451, 1536
Sau96I GGNCC 9 cut(s) 148, 271, 868, 1416, 1623, 1867, 1888, 1889, 1918
ScaI AGTACT 1 cut(s) 1588
SchI GAGTC 5 cut(s) 198, 371, 431, 509, 1019
ScrFI CCNGG 5 cut(s) 14, 773, 891, 1813, 1923
SduI GDGCHC 6 cut(s) 773, 1352, 1471, 1744, 1813, 1892
SfaNI GCATC 3 cut(s) 1098, 1469, 1695
SfoI GGCGCC 1 cut(s) 1656
SgrAI CRCCGGYG 1 cut(s) 1657
SinI GGWCC 4 cut(s) 148, 271, 1867, 1918
SmlI CTYRAG 1 cut(s) 1493
SmoI CTYRAG 1 cut(s) 1493
Sse9I AATT 2 cut(s) 607, 1087
SseBI AGGCCT 1 cut(s) 1391
SspDI GGCGCC 1 cut(s) 1654
SspMI CTAG 7 cut(s) 161, 170, 284, 293, 1101, 1263, 1805
SstI GAGCTC 1 cut(s) 1352
StuI AGGCCT 1 cut(s) 1391
StyD4I CCNGG 5 cut(s) 12, 771, 889, 1811, 1921
StyI CCWWGG 1 cut(s) 567
TaiI ACGT 6 cut(s) 76, 450, 557, 750, 1694, 1759
TasI AATT 2 cut(s) 607, 1087
TatI WGTACW 2 cut(s) 507, 1586
TfiI GAWTC 3 cut(s) 496, 1445, 1713
Tru1I TTAA 1 cut(s) 1718
Tru9I TTAA 1 cut(s) 1718
TscAI CASTG 4 cut(s) 346, 461, 829, 1488
TseFI GTSAC 3 cut(s) 638, 1175, 1370
TseI GCWGC 3 cut(s) 1155, 1402, 1543
Tsp45I GTSAC 3 cut(s) 638, 1175, 1370
TspGWI ACGGA 2 cut(s) 449, 867
TspRI CASTG 4 cut(s) 346, 461, 829, 1488
Van91I CCANNNNNTGG 1 cut(s) 1517
VpaK11BI GGWCC 4 cut(s) 148, 271, 1867, 1918
XcmI CCANNNNNNNNNTGG 2 cut(s) 103, 338
XmiI GTMKAC 2 cut(s) 318, 1121
XspI CTAG 7 cut(s) 161, 170, 284, 293, 1101, 1263, 1805
ZraI GACGTC 1 cut(s) 1692
ZrmI AGTACT 1 cut(s) 1588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.