RLG00000005487

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001823
Physical Location & Seq
Reverse (-)
68030820 .. 68031791
972 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005487

Sequence Viewer

Length: 972 bp
ATGGCTCTCATCCGGGAACGCCGCCAGCTCAACCTCAGCCTCCCCCTGCCGGAACCCTCCGAGCGTTGCCCACGTTTCACCCTACCTCTCCCTCCCACCGCCGCCGTGGCAACCACCACCAACTGCTCCTCCGCTGCCGCCTCCATCTCCGCCTCCGACCTCGAAAGACTAGAGGTACTAGGCCACGGTAACGGCGGCACAGTCTACAAAGTCCGCCACAAGCGCACTGGAGCTAACTACGCCCTGAAAATAGTGAAAGGCGACTCCGACCCCACCGTCCGCCGCCAGCTCTACCGCGAAATAGAAATCCTCCGCCGCACGGACTCCCCCCACGTGGTTCACTGCAACGCCATAATCGAGAAGCCCACCGGAGACATCTGGATTCTCATGGAGTACATGGACTCGGGAACATTAGAGACCTCGCTCAAAGAGAACGGCACGATCTCCGAGGCCAAGCTCGCTCATGTAGCTCGTCAAGTCCTCAACGGTCTCAATTACCTCCACGCTCAGAAGATCATCCACCGTGACATCAAGCCAGCCAATCTGTTAGTCAACAGCAACATGGAAGTCAAGATTGCCGACTTCGGCGTCAGCAAAATGATGTGCCGAACTCTCGATAACTGTAACTCGTACGTTGGGACTTGTGCTTACATGAGCCCGGAGAGATTTGACCCTGACACTTACGGTGGAAACTACAATGGATACGCCAGTGATATATGGAGCTTGGGTCTAACCCTCATGGAGCTTTACATGGGCCACTTTCCGTTCTTGCCTCCGGGTCAGAGACCCGACTGGGCGACCCTTATGTGCGCCATATGTTTCGGGGAGCCACCGGCCTTGCCGGAGGGGGTGTCCGAGGAGTTTAGGAGTTTTATGGAGTGTTGCTTGCAAAAGGAGTCGGGGAAGAGGTGGACGGCGGCTCAGCTTTTGACGCACCCGTTTGTCTGTAAAGATCCGAAAGCCATTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

324

Amino Acids

36.03

Weight (kDa)

7.55

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 55 - 315 6.3e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 55 - 310 1e-41 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 118 - 201 5e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 275, 587
AccI GTMKAC 1 cut(s) 204
AccII CGCG 1 cut(s) 297
AclWI GGATC 1 cut(s) 947
AcvI CACGTG 1 cut(s) 334
AcyI GRCGYC 1 cut(s) 588
AfaI GTAC 3 cut(s) 177, 395, 632
AfiI CCNNNNNNNGG 3 cut(s) 49, 319, 334
AluBI AGCT 8 cut(s) 28, 233, 289, 457, 470, 723, 745, 925
AluI AGCT 8 cut(s) 28, 233, 289, 457, 470, 723, 745, 925
Alw26I GTCTC 4 cut(s) 366, 410, 494, 778
AlwI GGATC 1 cut(s) 947
Ama87I CYCGRG 1 cut(s) 403
AoxI GGCC 4 cut(s) 181, 450, 754, 834
ApeKI GCWGC 1 cut(s) 134
AspLEI GCGC 2 cut(s) 225, 812
AspS9I GGNCC 1 cut(s) 754
AsuC2I CCSGG 3 cut(s) 14, 659, 777
AsuHPI GGTGA 1 cut(s) 70
AvaI CYCGRG 1 cut(s) 403
BaeI ACNNNNGTAYC 2 cut(s) 694, 727
BanII GRGCYC 1 cut(s) 659
BbrPI CACGTG 1 cut(s) 334
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 121
BccI CCATC 1 cut(s) 152
BceAI ACGGC 4 cut(s) 89, 208, 451, 930
BciVI GTATCC 1 cut(s) 695
BcnI CCSGG 3 cut(s) 14, 659, 777
BcoDI GTCTC 4 cut(s) 366, 410, 494, 778
BfaI CTAG 2 cut(s) 170, 179
BfuI GTATCC 1 cut(s) 695
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 8 cut(s) 22, 102, 135, 138, 196, 283, 316, 918
BlpI GCTNAGC 1 cut(s) 921
BlsI GCNGC 8 cut(s) 23, 103, 136, 139, 197, 284, 317, 919
Bme1390I CCNGG 3 cut(s) 14, 659, 777
BmeT110I CYCGRG 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 754
BmiI GGNNCC 2 cut(s) 54, 828
BmrFI CCNGG 3 cut(s) 14, 659, 777
BmrI ACTGGG 1 cut(s) 802
BmuI ACTGGG 1 cut(s) 802
BpmI CTGGAG 1 cut(s) 249
Bpu10I CCTNAGC 1 cut(s) 35
Bpu1102I GCTNAGC 1 cut(s) 921
BpuMI CCSGG 3 cut(s) 14, 659, 777
BsaAI YACGTR 1 cut(s) 334
BsaHI GRCGYC 1 cut(s) 588
BsaI GGTCTC 3 cut(s) 410, 494, 778
BsaJI CCNNGG 4 cut(s) 105, 184, 447, 855
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 6 cut(s) 113, 143, 712, 742, 836, 866
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 319, 334
Bse118I RCCGGY 1 cut(s) 832
Bse1I ACTGG 3 cut(s) 232, 708, 797
BseDI CCNNGG 4 cut(s) 105, 184, 447, 855
BseGI GGATG 2 cut(s) 9, 516
BseLI CCNNNNNNNGG 3 cut(s) 49, 319, 334
BseMII CTCAG 3 cut(s) 49, 521, 935
BseNI ACTGG 3 cut(s) 232, 708, 797
BseRI GAGGAG 2 cut(s) 118, 872
BseXI GCAGC 1 cut(s) 121
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 4 cut(s) 183, 452, 756, 836
BsiHKCI CYCGRG 1 cut(s) 403
BsiSI CCGG 7 cut(s) 13, 50, 369, 659, 776, 833, 842
BsiWI CGTACG 1 cut(s) 630
BslFI GGGAC 1 cut(s) 652
BslI CCNNNNNNNGG 3 cut(s) 49, 319, 334
BsmAI GTCTC 4 cut(s) 366, 410, 494, 778
BsmFI GGGAC 1 cut(s) 652
BsnI GGCC 4 cut(s) 183, 452, 756, 836
Bso31I GGTCTC 3 cut(s) 410, 494, 778
BsoBI CYCGRG 1 cut(s) 403
Bsp1286I GDGCHC 1 cut(s) 659
Bsp143I GATC 3 cut(s) 441, 513, 952
Bsp1720I GCTNAGC 1 cut(s) 921
BspANI GGCC 4 cut(s) 183, 452, 756, 836
BspCNI CTCAG 3 cut(s) 48, 520, 934
BspFNI CGCG 1 cut(s) 297
BspLI GGNNCC 2 cut(s) 54, 828
BspPI GGATC 1 cut(s) 947
BspTNI GGTCTC 3 cut(s) 410, 494, 778
BsrFI RCCGGY 1 cut(s) 832
BsrI ACTGG 3 cut(s) 232, 708, 797
BssAI RCCGGY 1 cut(s) 832
BssECI CCNNGG 4 cut(s) 105, 184, 447, 855
BssMI GATC 3 cut(s) 441, 513, 952
BssNI GRCGYC 1 cut(s) 588
Bst4CI ACNGT 7 cut(s) 188, 202, 277, 488, 524, 623, 686
Bst6I CTCTTC 1 cut(s) 899
BstACI GRCGYC 1 cut(s) 588
BstBAI YACGTR 1 cut(s) 334
BstC8I GCNNGC 5 cut(s) 26, 287, 459, 537, 887
BstDEI CTNAG 3 cut(s) 35, 507, 921
BstDSI CCRYGG 2 cut(s) 105, 184
BstF5I GGATG 2 cut(s) 9, 516
BstFNI CGCG 1 cut(s) 297
BstHHI GCGC 2 cut(s) 225, 812
BstKTI GATC 3 cut(s) 444, 516, 955
BstMAI GTCTC 4 cut(s) 366, 410, 494, 778
BstMBI GATC 3 cut(s) 441, 513, 952
BstMWI GCNNNNNNNGC 6 cut(s) 107, 222, 239, 458, 467, 931
BstSCI CCNGG 3 cut(s) 12, 657, 775
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 1 cut(s) 121
BstX2I RGATCY 1 cut(s) 952
BstYI RGATCY 1 cut(s) 952
BsuI GTATCC 1 cut(s) 695
BsuRI GGCC 4 cut(s) 183, 452, 756, 836
BtgI CCRYGG 2 cut(s) 105, 184
BtsCI GGATG 2 cut(s) 9, 516
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 3 cut(s) 225, 340, 715
Cac8I GCNNGC 5 cut(s) 26, 287, 459, 537, 887
CfoI GCGC 2 cut(s) 225, 812
Cfr10I RCCGGY 1 cut(s) 832
Cfr13I GGNCC 1 cut(s) 754
CseI GACGC 2 cut(s) 577, 940
Csp6I GTAC 3 cut(s) 176, 394, 631
CspCI CAANNNNNGTGG 2 cut(s) 819, 854
CviAII CATG 7 cut(s) 388, 397, 464, 562, 652, 739, 751
CviQI GTAC 3 cut(s) 176, 394, 631
DdeI CTNAG 3 cut(s) 35, 507, 921
DpnI GATC 3 cut(s) 443, 515, 954
DpnII GATC 3 cut(s) 441, 513, 952
DrdI GACNNNNNNGTC 2 cut(s) 275, 587
DseDI GACNNNNNNGTC 2 cut(s) 275, 587
Eam1104I CTCTTC 1 cut(s) 899
EarI CTCTTC 1 cut(s) 899
EciI GGCGGA 4 cut(s) 139, 203, 269, 302
Eco24I GRGCYC 1 cut(s) 659
Eco31I GGTCTC 3 cut(s) 410, 494, 778
Eco72I CACGTG 1 cut(s) 334
Eco88I CYCGRG 1 cut(s) 403
EcoT38I GRGCYC 1 cut(s) 659
FaeI CATG 7 cut(s) 391, 400, 467, 565, 655, 742, 754
FaqI GGGAC 1 cut(s) 652
FatI CATG 7 cut(s) 387, 396, 463, 561, 651, 738, 750
FauNDI CATATG 1 cut(s) 815
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 8 cut(s) 22, 102, 135, 138, 196, 283, 316, 918
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 659
Fsp4HI GCNGC 8 cut(s) 22, 102, 135, 138, 196, 283, 316, 918
FspBI CTAG 2 cut(s) 170, 179
GlaI GCGC 2 cut(s) 224, 811
GluI GCNGC 8 cut(s) 22, 102, 135, 138, 196, 283, 316, 918
GsuI CTGGAG 1 cut(s) 249
HaeIII GGCC 4 cut(s) 183, 452, 756, 836
HapII CCGG 7 cut(s) 13, 50, 369, 659, 776, 833, 842
HgaI GACGC 2 cut(s) 577, 940
HhaI GCGC 2 cut(s) 225, 812
Hin1I GRCGYC 1 cut(s) 588
Hin1II CATG 7 cut(s) 391, 400, 467, 565, 655, 742, 754
Hin6I GCGC 2 cut(s) 223, 810
HinP1I GCGC 2 cut(s) 223, 810
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HinfI GANTC 5 cut(s) 263, 323, 382, 401, 896
HpaII CCGG 7 cut(s) 13, 50, 369, 659, 776, 833, 842
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 4 cut(s) 205, 340, 553, 912
Hpy188I TCNGA 8 cut(s) 61, 157, 268, 448, 510, 783, 856, 957
Hpy188III TCNNGA 5 cut(s) 358, 379, 405, 571, 614
Hpy8I GTNNAC 4 cut(s) 205, 340, 553, 912
HpyCH4III ACNGT 7 cut(s) 188, 202, 277, 488, 524, 623, 686
HpyCH4IV ACGT 3 cut(s) 73, 333, 633
HpyCH4V TGCA 2 cut(s) 345, 889
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 222, 239, 458, 467, 931
HpyF3I CTNAG 3 cut(s) 35, 507, 921
HpySE526I ACGT 3 cut(s) 73, 333, 633
Hsp92I GRCGYC 1 cut(s) 588
Hsp92II CATG 7 cut(s) 391, 400, 467, 565, 655, 742, 754
HspAI GCGC 2 cut(s) 223, 810
Kzo9I GATC 3 cut(s) 441, 513, 952
LmnI GCTCC 5 cut(s) 131, 230, 720, 742, 826
Lsp1109I GCAGC 1 cut(s) 121
MaeI CTAG 2 cut(s) 170, 179
MaeII ACGT 3 cut(s) 73, 333, 633
MaeIII GTNAC 3 cut(s) 188, 524, 623
MalI GATC 3 cut(s) 443, 515, 954
MboI GATC 3 cut(s) 441, 513, 952
MboII GAAGA 2 cut(s) 523, 916
MflI RGATCY 1 cut(s) 952
MhlI GDGCHC 1 cut(s) 659
MluCI AATT 1 cut(s) 493
MlyI GAGTC 4 cut(s) 257, 317, 395, 905
MmeI TCCRAC 2 cut(s) 180, 291
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 7 cut(s) 13, 50, 369, 659, 776, 833, 842
MspR9I CCNGG 3 cut(s) 14, 659, 777
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 6 cut(s) 107, 222, 239, 458, 467, 931
NciI CCSGG 3 cut(s) 14, 659, 777
NdeI CATATG 1 cut(s) 815
NdeII GATC 3 cut(s) 441, 513, 952
NlaIII CATG 7 cut(s) 391, 400, 467, 565, 655, 742, 754
NlaIV GGNNCC 2 cut(s) 54, 828
NmuCI GTSAC 1 cut(s) 524
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PfeI GAWTC 1 cut(s) 382
Pfl23II CGTACG 1 cut(s) 630
PfoI TCCNGGA 1 cut(s) 12
PkrI GCNGC 8 cut(s) 23, 103, 136, 139, 197, 284, 317, 919
PleI GAGTC 4 cut(s) 257, 317, 395, 904
PmaCI CACGTG 1 cut(s) 334
PmlI CACGTG 1 cut(s) 334
PpsI GAGTC 4 cut(s) 257, 317, 395, 904
Ppu21I YACGTR 1 cut(s) 334
PspCI CACGTG 1 cut(s) 334
PspLI CGTACG 1 cut(s) 630
PspN4I GGNNCC 2 cut(s) 54, 828
PspPI GGNCC 1 cut(s) 754
PsuI RGATCY 1 cut(s) 952
RsaI GTAC 3 cut(s) 177, 395, 632
RsaNI GTAC 3 cut(s) 176, 394, 631
SatI GCNGC 8 cut(s) 22, 102, 135, 138, 196, 283, 316, 918
Sau3AI GATC 3 cut(s) 441, 513, 952
Sau96I GGNCC 1 cut(s) 754
SchI GAGTC 4 cut(s) 257, 317, 395, 905
ScrFI CCNGG 3 cut(s) 14, 659, 777
SduI GDGCHC 1 cut(s) 659
Sse9I AATT 1 cut(s) 493
SspMI CTAG 2 cut(s) 170, 179
StyD4I CCNGG 3 cut(s) 12, 657, 775
TaaI ACNGT 7 cut(s) 188, 202, 277, 488, 524, 623, 686
TaiI ACGT 3 cut(s) 76, 336, 636
TaqI TCGA 3 cut(s) 162, 357, 615
TasI AATT 1 cut(s) 493
TatI WGTACW 1 cut(s) 393
TauI GCSGC 7 cut(s) 24, 104, 140, 198, 285, 318, 920
TfiI GAWTC 1 cut(s) 382
TscAI CASTG 3 cut(s) 232, 347, 715
TseFI GTSAC 1 cut(s) 524
TseI GCWGC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 524
TspGWI ACGGA 2 cut(s) 335, 753
TspRI CASTG 3 cut(s) 232, 347, 715
XcmI CCANNNNNNNNNTGG 2 cut(s) 103, 224
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 2 cut(s) 170, 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.