Prupe.1G082800_v2.0.a1

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001823
Physical Location & Seq
Forward (+)
6143647 .. 6144612
966 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G082800.1

Sequence Viewer

Length: 966 bp
ATGGCTCTCATTCAACAACGCCGCCAGCTTAATCTCCGCCTCCCCTTTCCCGAACCCTCCGAGTGCCGCCCATGTTTCTCCGTTCCCCTTCCTCCCACCACCGCCGTCACAAACAAATCTTCCTTCGGTGCTATCTCCGCCGCCGATATCGAGAAACTCCAAGTCCTCGGCTACGGTAACAGCGGCACCGTCTACAAGGTAAATCACAAGCGGACTTCCACCACCTACGCTCTCAAACTCGTCCATAGCGACTCCAACGACTCCACAGTCCGCCGTCAGCTCTTCCACGAGATGGAGATCCTCCGCCACACCGACTCTCCCCATGTCGTCCGCTGCCACGCTATCTTTGAGAAGTCGTCGGGAGATATCGGGATCCTCATAGAGTACATAGACTCCGGCACCCTCGAGACCTTGCTTAAAGCCCAAGGCACCTTCTCCGAGCCCAATCTTGCCCACGTCGCCCGCCAAGTTCTTAAAGGCCTCAACTACCTCCACACCAACAAGATCATCCACCGCAACATCAAACCCGCAAATGTTTTGGTGAACAGCAACATGGAAGTGAAAATCGCCGACTTTGGCGTGAGCAAGATCCTATGCCTGACCTCGGACCCCTGCAATTCCTATGTGGGTACTTGTGCTTACATGAGCCCGGAAAGATTTGACCCGGATACTTACGGCGGCAATTACAGTGGCTACGCCGGTGACATATGGAGCCTAGGGCTAACCCTGATGGAGCTTTACATGGGTCACTTTCCGTTGTTGCCTCCGGGTCAGAAACCCGACTGGGCCACGCTTATGTGCGCCATATGTTTTGGAGAGCCGCCGAGCTTGCCCGAGGGGGTGTCGGAGGAGTTTCGGAGTTTCATGGAATGTTGCTTGCAGATGGAGTCTGAAATGCGGTGGACCGCCGCTCAGCTTTTGACCCACCCGTTCGTCTCTAAAGATCCCAGTATATCCGTTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

322

Amino Acids

35.68

Weight (kDa)

6.26

Isoelectric Point (pI)

55.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 266
AccB1I GGYRCC 3 cut(s) 185, 398, 428
AccB7I CCANNNNNTGG 1 cut(s) 292
AccBSI CCGCTC 1 cut(s) 911
AccI GTMKAC 1 cut(s) 192
AclWI GGATC 5 cut(s) 292, 367, 380, 583, 938
AfaI GTAC 2 cut(s) 386, 631
AfiI CCNNNNNNNGG 2 cut(s) 292, 604
AgsI TTSAA 1 cut(s) 14
AjiI CACGTC 1 cut(s) 457
AluBI AGCT 5 cut(s) 28, 280, 736, 828, 916
AluI AGCT 5 cut(s) 28, 280, 736, 828, 916
Alw26I GTCTC 2 cut(s) 401, 940
AlwI GGATC 5 cut(s) 292, 367, 380, 583, 938
Ama87I CYCGRG 2 cut(s) 404, 833
AoxI GGCC 2 cut(s) 478, 786
ApeKI GCWGC 1 cut(s) 333
AspA2I CCTAGG 1 cut(s) 715
AspLEI GCGC 1 cut(s) 803
AspS9I GGNCC 3 cut(s) 607, 786, 903
AsuC2I CCSGG 3 cut(s) 650, 665, 768
AsuHPI GGTGA 2 cut(s) 553, 713
AvaI CYCGRG 2 cut(s) 404, 833
AvaII GGWCC 2 cut(s) 607, 903
AvrII CCTAGG 1 cut(s) 715
BamHI GGATCC 1 cut(s) 372
BanI GGYRCC 3 cut(s) 185, 398, 428
BanII GRGCYC 2 cut(s) 444, 650
BauI CACGAG 1 cut(s) 287
BbvI GCAGC 1 cut(s) 320
BccI CCATC 3 cut(s) 286, 724, 877
BceAI ACGGC 3 cut(s) 89, 258, 691
BciVI GTATCC 1 cut(s) 661
BcnI CCSGG 3 cut(s) 650, 665, 768
BcoDI GTCTC 2 cut(s) 401, 940
BfaI CTAG 1 cut(s) 716
BfuI GTATCC 1 cut(s) 661
BisI GCNGC 8 cut(s) 22, 67, 141, 184, 334, 679, 821, 909
BlnI CCTAGG 1 cut(s) 715
BlpI GCTNAGC 1 cut(s) 912
BlsI GCNGC 8 cut(s) 23, 68, 142, 185, 335, 680, 822, 910
Bme1390I CCNGG 3 cut(s) 650, 665, 768
Bme18I GGWCC 2 cut(s) 607, 903
BmeT110I CYCGRG 2 cut(s) 404, 833
BmgBI CACGTC 1 cut(s) 457
BmgT120I GGNCC 3 cut(s) 607, 786, 903
BmiI GGNNCC 6 cut(s) 187, 374, 400, 430, 609, 713
BmrFI CCNGG 3 cut(s) 650, 665, 768
BmrI ACTGGG 2 cut(s) 793, 942
BmuI ACTGGG 2 cut(s) 793, 942
Bpu1102I GCTNAGC 1 cut(s) 912
BpuMI CCSGG 3 cut(s) 650, 665, 768
BsaBI GATNNNNATC 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 5 cut(s) 166, 424, 603, 715, 834
BsaXI ACNNNNNCTCC 4 cut(s) 301, 331, 377, 407
Bsc4I CCNNNNNNNGG 2 cut(s) 292, 604
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 2 cut(s) 788, 948
Bse8I GATNNNNATC 1 cut(s) 296
BseDI CCNNGG 5 cut(s) 166, 424, 603, 715, 834
BseGI GGATG 1 cut(s) 507
BseJI GATNNNNATC 1 cut(s) 296
BseLI CCNNNNNNNGG 2 cut(s) 292, 604
BseMII CTCAG 1 cut(s) 926
BseNI ACTGG 2 cut(s) 788, 948
BseRI GAGGAG 1 cut(s) 863
BseXI GCAGC 1 cut(s) 320
BshFI GGCC 2 cut(s) 480, 788
BshNI GGYRCC 3 cut(s) 185, 398, 428
BsiHKCI CYCGRG 2 cut(s) 404, 833
BsiSI CCGG 5 cut(s) 396, 650, 665, 699, 767
BslI CCNNNNNNNGG 2 cut(s) 292, 604
BsmAI GTCTC 2 cut(s) 401, 940
BsmBI CGTCTC 1 cut(s) 940
BsnI GGCC 2 cut(s) 480, 788
Bso31I GGTCTC 1 cut(s) 401
BsoBI CYCGRG 2 cut(s) 404, 833
Bsp1286I GDGCHC 2 cut(s) 444, 650
Bsp143I GATC 5 cut(s) 297, 372, 504, 588, 943
Bsp1720I GCTNAGC 1 cut(s) 912
BspANI GGCC 2 cut(s) 480, 788
BspCNI CTCAG 1 cut(s) 925
BspLI GGNNCC 6 cut(s) 187, 374, 400, 430, 609, 713
BspPI GGATC 5 cut(s) 292, 367, 380, 583, 938
BspQI GCTCTTC 1 cut(s) 287
BspT107I GGYRCC 3 cut(s) 185, 398, 428
BspTNI GGTCTC 1 cut(s) 401
BsrBI CCGCTC 1 cut(s) 911
BsrFI RCCGGY 1 cut(s) 698
BsrI ACTGG 2 cut(s) 788, 948
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 5 cut(s) 166, 424, 603, 715, 834
BssMI GATC 5 cut(s) 297, 372, 504, 588, 943
BssSI CACGAG 1 cut(s) 287
BssT1I CCWWGG 2 cut(s) 424, 715
Bst2BI CACGAG 1 cut(s) 287
Bst4CI ACNGT 4 cut(s) 176, 190, 268, 689
Bst6I CTCTTC 1 cut(s) 287
BstC8I GCNNGC 4 cut(s) 26, 463, 830, 878
BstDEI CTNAG 1 cut(s) 912
BstF5I GGATG 1 cut(s) 507
BstHHI GCGC 1 cut(s) 803
BstKTI GATC 5 cut(s) 300, 375, 507, 591, 946
BstMAI GTCTC 2 cut(s) 401, 940
BstMBI GATC 5 cut(s) 297, 372, 504, 588, 943
BstMWI GCNNNNNNNGC 3 cut(s) 137, 458, 829
BstSCI CCNGG 3 cut(s) 648, 663, 766
BstV1I GCAGC 1 cut(s) 320
BstX2I RGATCY 4 cut(s) 297, 372, 588, 943
BstYI RGATCY 4 cut(s) 297, 372, 588, 943
BsuI GTATCC 1 cut(s) 661
BsuRI GGCC 2 cut(s) 480, 788
BtrI CACGTC 1 cut(s) 457
BtsCI GGATG 1 cut(s) 507
BtsIMutI CAGTG 1 cut(s) 694
Cac8I GCNNGC 4 cut(s) 26, 463, 830, 878
CfoI GCGC 1 cut(s) 803
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 3 cut(s) 607, 786, 903
Csp6I GTAC 2 cut(s) 385, 630
CspCI CAANNNNNGTGG 2 cut(s) 670, 705
CviAII CATG 6 cut(s) 72, 323, 553, 643, 742, 865
CviQI GTAC 2 cut(s) 385, 630
DdeI CTNAG 1 cut(s) 912
DpnI GATC 5 cut(s) 299, 374, 506, 590, 945
DpnII GATC 5 cut(s) 297, 372, 504, 588, 943
DrdI GACNNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 266
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
EciI GGCGGA 4 cut(s) 26, 127, 260, 293
Eco130I CCWWGG 2 cut(s) 424, 715
Eco147I AGGCCT 1 cut(s) 480
Eco24I GRGCYC 2 cut(s) 444, 650
Eco31I GGTCTC 1 cut(s) 401
Eco32I GATATC 2 cut(s) 148, 367
Eco47I GGWCC 2 cut(s) 607, 903
Eco88I CYCGRG 2 cut(s) 404, 833
EcoRV GATATC 2 cut(s) 148, 367
EcoT14I CCWWGG 2 cut(s) 424, 715
EcoT38I GRGCYC 2 cut(s) 444, 650
ErhI CCWWGG 2 cut(s) 424, 715
Esp3I CGTCTC 1 cut(s) 940
FaeI CATG 6 cut(s) 75, 326, 556, 646, 745, 868
FatI CATG 6 cut(s) 71, 322, 552, 642, 741, 864
FauI CCCGC 2 cut(s) 470, 535
FauNDI CATATG 2 cut(s) 707, 806
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 8 cut(s) 22, 67, 141, 184, 334, 679, 821, 909
FokI GGATG 1 cut(s) 494
FriOI GRGCYC 2 cut(s) 444, 650
Fsp4HI GCNGC 8 cut(s) 22, 67, 141, 184, 334, 679, 821, 909
FspBI CTAG 1 cut(s) 716
GlaI GCGC 1 cut(s) 802
GluI GCNGC 8 cut(s) 22, 67, 141, 184, 334, 679, 821, 909
HaeIII GGCC 2 cut(s) 480, 788
HapII CCGG 5 cut(s) 396, 650, 665, 699, 767
HhaI GCGC 1 cut(s) 803
Hin1II CATG 6 cut(s) 75, 326, 556, 646, 745, 868
Hin6I GCGC 1 cut(s) 801
HinP1I GCGC 1 cut(s) 801
HinfI GANTC 5 cut(s) 251, 260, 314, 392, 887
HpaII CCGG 5 cut(s) 396, 650, 665, 699, 767
HphI GGTGA 2 cut(s) 553, 713
Hpy166II GTNNAC 3 cut(s) 193, 544, 903
Hpy188I TCNGA 7 cut(s) 61, 439, 607, 774, 847, 858, 892
Hpy188III TCNNGA 6 cut(s) 50, 151, 360, 370, 406, 963
Hpy8I GTNNAC 3 cut(s) 193, 544, 903
Hpy99I CGWCG 2 cut(s) 361, 461
HpyAV CCTTC 3 cut(s) 98, 133, 442
HpyCH4III ACNGT 4 cut(s) 176, 190, 268, 689
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 2 cut(s) 615, 880
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 458, 829
HpyF3I CTNAG 1 cut(s) 912
HpySE526I ACGT 1 cut(s) 456
Hsp92II CATG 6 cut(s) 75, 326, 556, 646, 745, 868
HspAI GCGC 1 cut(s) 801
Kzo9I GATC 5 cut(s) 297, 372, 504, 588, 943
LguI GCTCTTC 1 cut(s) 287
LmnI GCTCC 2 cut(s) 711, 733
Lsp1109I GCAGC 1 cut(s) 320
MaeI CTAG 1 cut(s) 716
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 4 cut(s) 106, 176, 701, 746
MalI GATC 5 cut(s) 299, 374, 506, 590, 945
MbiI CCGCTC 1 cut(s) 911
MboI GATC 5 cut(s) 297, 372, 504, 588, 943
MboII GAAGA 2 cut(s) 111, 274
MflI RGATCY 4 cut(s) 297, 372, 588, 943
MhlI GDGCHC 2 cut(s) 444, 650
MluCI AATT 2 cut(s) 616, 682
MlyI GAGTC 5 cut(s) 245, 254, 308, 386, 896
MmeI TCCRAC 2 cut(s) 279, 825
MseI TTAA 3 cut(s) 30, 417, 474
MslI CAYNNNNRTG 2 cut(s) 557, 794
MspA1I CMGCKG 2 cut(s) 183, 333
MspI CCGG 5 cut(s) 396, 650, 665, 699, 767
MspR9I CCNGG 3 cut(s) 650, 665, 768
MwoI GCNNNNNNNGC 3 cut(s) 137, 458, 829
NciI CCSGG 3 cut(s) 650, 665, 768
NdeI CATATG 2 cut(s) 707, 806
NdeII GATC 5 cut(s) 297, 372, 504, 588, 943
NlaIII CATG 6 cut(s) 75, 326, 556, 646, 745, 868
NlaIV GGNNCC 6 cut(s) 187, 374, 400, 430, 609, 713
NmeAIII GCCGAG 2 cut(s) 147, 849
NmuCI GTSAC 3 cut(s) 106, 701, 746
PaeR7I CTCGAG 1 cut(s) 404
PceI AGGCCT 1 cut(s) 480
PciSI GCTCTTC 1 cut(s) 287
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PflMI CCANNNNNTGG 1 cut(s) 292
PkrI GCNGC 8 cut(s) 23, 68, 142, 185, 335, 680, 822, 910
PleI GAGTC 5 cut(s) 245, 254, 308, 386, 895
PpsI GAGTC 5 cut(s) 245, 254, 308, 386, 895
PspN4I GGNNCC 6 cut(s) 187, 374, 400, 430, 609, 713
PspPI GGNCC 3 cut(s) 607, 786, 903
PsuI RGATCY 4 cut(s) 297, 372, 588, 943
RsaI GTAC 2 cut(s) 386, 631
RsaNI GTAC 2 cut(s) 385, 630
RseI CAYNNNNRTG 2 cut(s) 557, 794
SapI GCTCTTC 1 cut(s) 287
SaqAI TTAA 3 cut(s) 30, 417, 474
SatI GCNGC 8 cut(s) 22, 67, 141, 184, 334, 679, 821, 909
Sau3AI GATC 5 cut(s) 297, 372, 504, 588, 943
Sau96I GGNCC 3 cut(s) 607, 786, 903
SchI GAGTC 5 cut(s) 245, 254, 308, 386, 896
ScrFI CCNGG 3 cut(s) 650, 665, 768
SduI GDGCHC 2 cut(s) 444, 650
Sfr274I CTCGAG 1 cut(s) 404
SgrAI CRCCGGYG 1 cut(s) 698
SinI GGWCC 2 cut(s) 607, 903
SlaI CTCGAG 1 cut(s) 404
SmiMI CAYNNNNRTG 2 cut(s) 557, 794
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 2 cut(s) 616, 682
SseBI AGGCCT 1 cut(s) 480
SspMI CTAG 1 cut(s) 716
StuI AGGCCT 1 cut(s) 480
StyD4I CCNGG 3 cut(s) 648, 663, 766
StyI CCWWGG 2 cut(s) 424, 715
TaaI ACNGT 4 cut(s) 176, 190, 268, 689
TaiI ACGT 1 cut(s) 459
TaqI TCGA 2 cut(s) 150, 405
TasI AATT 2 cut(s) 616, 682
TatI WGTACW 1 cut(s) 384
TauI GCSGC 7 cut(s) 24, 69, 143, 186, 681, 823, 911
Tru1I TTAA 3 cut(s) 30, 417, 474
Tru9I TTAA 3 cut(s) 30, 417, 474
TscAI CASTG 1 cut(s) 694
TseFI GTSAC 3 cut(s) 106, 701, 746
TseI GCWGC 1 cut(s) 333
Tsp45I GTSAC 3 cut(s) 106, 701, 746
TspDTI ATGAA 1 cut(s) 853
TspGWI ACGGA 3 cut(s) 70, 744, 946
TspRI CASTG 1 cut(s) 694
Van91I CCANNNNNTGG 1 cut(s) 292
VpaK11BI GGWCC 2 cut(s) 607, 903
XhoI CTCGAG 1 cut(s) 404
XmaJI CCTAGG 1 cut(s) 715
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.