Rorug06G0411800

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001823
Physical Location & Seq
Forward (+)
55357514 .. 55358850
1337 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0411800.1

Sequence Viewer

Length: 609 bp
ATGAAAGACGAAGATGCACCACCGACACTCGTCTCAATCCCCTCTCCAACCACCGCCGCAACTCCGCTGATCAGGAAACCGGAGAGCTCACCCTCGTCTTCTTCAAAAGCAGCTCGGCTGTTTGTTGTCAAGAATCGCTACAAGTTCTGGGCTCTAGCGGCGATTCTGTTGCTAGCTTTCTGGTCTATGTTCACCGGCTCCGTTACGCTCAGATGGTCTGCCGGCAACTTAACCGGCCTTTCCGACCAGCTCAACCTCCCGAGTTACGACGATCTTGATATTCTGGAAGTGGAGGACAGAGAGAAGGTGGTGAGGCACTTGTGGGATTTGTACACGCAGAGGCAGACGGGGGGGTCGAGCAGAAGAAGCACGACCCGACTGCCTCGGTTTTGGCAGGAGGCTTTTGAAGCTGCTTATGATCCTTTGAGCAGTGATGTTCCTGCTGTCAGAAACGCTGCCGTTTCTGAGATTGCTAAGATGTCCATTCGCTCCATCAATGTTGTTGACCCCTTCCCACTCCAATCCAAGGGCGTTAGGGAATTGAAAAGGAAGATCAAGAAAACAAAGGAAAACAAGGAGGTAACTCATACAGTAGGGAGCAACTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

202

Amino Acids

22.59

Weight (kDa)

9.69

Isoelectric Point (pI)

58.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1195 PF06708 15 - 170 1.1e-60 Protein of unknown function (DUF1195)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 352
AciI CCGC 4 cut(s) 54, 57, 65, 158
AclWI GGATC 1 cut(s) 413
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 1 cut(s) 526
AgsI TTSAA 3 cut(s) 105, 407, 544
AluBI AGCT 5 cut(s) 87, 113, 176, 250, 410
AluI AGCT 5 cut(s) 87, 113, 176, 250, 410
Alw21I GWGCWC 1 cut(s) 89
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 1 cut(s) 413
Ama87I CYCGRG 1 cut(s) 259
AoxI GGCC 1 cut(s) 235
ApeKI GCWGC 3 cut(s) 110, 410, 455
AsuHPI GGTGA 3 cut(s) 81, 184, 322
AsuNHI GCTAGC 1 cut(s) 172
AvaI CYCGRG 1 cut(s) 259
BanII GRGCYC 2 cut(s) 89, 154
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 89
BbvI GCAGC 3 cut(s) 122, 397, 442
BccI CCATC 2 cut(s) 207, 500
BceAI ACGGC 1 cut(s) 443
BcgI CGANNNNNNTGC 4 cut(s) 151, 185, 361, 395
BclI TGATCA 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 2 cut(s) 155, 173
BisI GCNGC 5 cut(s) 57, 111, 159, 411, 456
BlsI GCNGC 5 cut(s) 58, 112, 160, 412, 457
BmeT110I CYCGRG 1 cut(s) 259
BmiI GGNNCC 1 cut(s) 199
BmsI GCATC 1 cut(s) 4
BmtI GCTAGC 1 cut(s) 176
BoxI GACNNNNGTC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 383, 525
BsaWI WCCGGW 1 cut(s) 79
Bsc4I CCNNNNNNNGG 1 cut(s) 526
Bse118I RCCGGY 3 cut(s) 194, 221, 233
BseDI CCNNGG 2 cut(s) 383, 525
BseLI CCNNNNNNNGG 1 cut(s) 526
BseMII CTCAG 2 cut(s) 223, 456
BseXI GCAGC 3 cut(s) 122, 397, 442
BshFI GGCC 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 89
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 4 cut(s) 80, 195, 222, 234
BslI CCNNNNNNNGG 1 cut(s) 526
BsmAI GTCTC 1 cut(s) 37
BsmBI CGTCTC 1 cut(s) 37
BsnI GGCC 1 cut(s) 237
BsoBI CYCGRG 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 89, 154
Bsp1407I TGTACA 1 cut(s) 330
Bsp143I GATC 4 cut(s) 69, 271, 418, 552
BspACI CCGC 4 cut(s) 54, 57, 65, 158
BspANI GGCC 1 cut(s) 237
BspCNI CTCAG 2 cut(s) 222, 457
BspLI GGNNCC 1 cut(s) 199
BspOI GCTAGC 1 cut(s) 176
BspPI GGATC 1 cut(s) 413
BsrFI RCCGGY 3 cut(s) 194, 221, 233
BsrGI TGTACA 1 cut(s) 330
BssAI RCCGGY 3 cut(s) 194, 221, 233
BssECI CCNNGG 2 cut(s) 383, 525
BssMI GATC 4 cut(s) 69, 271, 418, 552
BssT1I CCWWGG 1 cut(s) 525
Bst4CI ACNGT 1 cut(s) 592
BstAUI TGTACA 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 174, 223
BstDEI CTNAG 3 cut(s) 209, 465, 474
BstKTI GATC 4 cut(s) 72, 274, 421, 555
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 4 cut(s) 69, 271, 418, 552
BstMWI GCNNNNNNNGC 3 cut(s) 158, 366, 407
BstPAI GACNNNNGTC 1 cut(s) 29
BstV1I GCAGC 3 cut(s) 122, 397, 442
BstV2I GAAGAC 1 cut(s) 90
BsuRI GGCC 1 cut(s) 237
BtsI GCAGTG 1 cut(s) 436
BtsIMutI CAGTG 1 cut(s) 436
Cac8I GCNNGC 2 cut(s) 174, 223
Cfr10I RCCGGY 3 cut(s) 194, 221, 233
Csp6I GTAC 1 cut(s) 331
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 3 cut(s) 209, 465, 474
DpnI GATC 4 cut(s) 71, 273, 420, 554
DpnII GATC 4 cut(s) 69, 271, 418, 552
DrdI GACNNNNNNGTC 1 cut(s) 352
DseDI GACNNNNNNGTC 1 cut(s) 352
Ecl136II GAGCTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 525
Eco24I GRGCYC 2 cut(s) 89, 154
Eco53kI GAGCTC 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 259
EcoICRI GAGCTC 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 525
EcoT38I GRGCYC 2 cut(s) 89, 154
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 1 cut(s) 37
FaiI YATR 4 cut(s) 188, 417, 588, 607
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 5 cut(s) 57, 111, 159, 411, 456
FriOI GRGCYC 2 cut(s) 89, 154
Fsp4HI GCNGC 5 cut(s) 57, 111, 159, 411, 456
FspBI CTAG 2 cut(s) 155, 173
GluI GCNGC 5 cut(s) 57, 111, 159, 411, 456
HaeIII GGCC 1 cut(s) 237
HapII CCGG 4 cut(s) 80, 195, 222, 234
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 2 cut(s) 133, 163
HpaII CCGG 4 cut(s) 80, 195, 222, 234
HphI GGTGA 3 cut(s) 81, 184, 322
Hpy166II GTNNAC 3 cut(s) 192, 333, 505
Hpy188I TCNGA 4 cut(s) 212, 244, 449, 466
Hpy188III TCNNGA 6 cut(s) 73, 130, 259, 275, 284, 556
Hpy8I GTNNAC 3 cut(s) 192, 333, 505
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 2 cut(s) 298, 520
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4V TGCA 1 cut(s) 17
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 366, 407
HpyF3I CTNAG 3 cut(s) 209, 465, 474
KroI GCCGGC 1 cut(s) 221
KroNI GCCGGC 1 cut(s) 223
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 4 cut(s) 69, 271, 418, 552
LmnI GCTCC 3 cut(s) 203, 494, 597
Lsp1109I GCAGC 3 cut(s) 122, 397, 442
LweI GCATC 1 cut(s) 4
MaeI CTAG 2 cut(s) 155, 173
MaeIII GTNAC 3 cut(s) 202, 263, 580
MalI GATC 4 cut(s) 71, 273, 420, 554
MboI GATC 4 cut(s) 69, 271, 418, 552
MboII GAAGA 5 cut(s) 23, 90, 93, 375, 562
MhlI GDGCHC 2 cut(s) 89, 154
MluCI AATT 1 cut(s) 539
MmeI TCCRAC 2 cut(s) 71, 267
MnlI CCTC 9 cut(s) 52, 103, 266, 286, 306, 333, 391, 393, 571
MroNI GCCGGC 1 cut(s) 221
MseI TTAA 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 4 cut(s) 80, 195, 222, 234
MwoI GCNNNNNNNGC 3 cut(s) 158, 366, 407
NaeI GCCGGC 1 cut(s) 223
NdeII GATC 4 cut(s) 69, 271, 418, 552
NgoMIV GCCGGC 1 cut(s) 221
NheI GCTAGC 1 cut(s) 172
NlaIV GGNNCC 1 cut(s) 199
NmeAIII GCCGAG 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 353
PdiI GCCGGC 1 cut(s) 223
PfeI GAWTC 2 cut(s) 133, 163
PkrI GCNGC 5 cut(s) 58, 112, 160, 412, 457
PshAI GACNNNNGTC 1 cut(s) 29
Psp124BI GAGCTC 1 cut(s) 89
PspN4I GGNNCC 1 cut(s) 199
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SacI GAGCTC 1 cut(s) 89
SaqAI TTAA 1 cut(s) 230
SatI GCNGC 5 cut(s) 57, 111, 159, 411, 456
Sau3AI GATC 4 cut(s) 69, 271, 418, 552
SduI GDGCHC 2 cut(s) 89, 154
SetI ASST 8 cut(s) 89, 115, 178, 252, 258, 309, 412, 582
SfaNI GCATC 1 cut(s) 4
Sse9I AATT 1 cut(s) 539
SsiI CCGC 4 cut(s) 54, 57, 65, 158
SspMI CTAG 2 cut(s) 155, 173
SstI GAGCTC 1 cut(s) 89
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 1 cut(s) 592
TaqI TCGA 1 cut(s) 356
TasI AATT 1 cut(s) 539
TatI WGTACW 1 cut(s) 330
TauI GCSGC 2 cut(s) 59, 161
TfiI GAWTC 2 cut(s) 133, 163
Tru1I TTAA 1 cut(s) 230
Tru9I TTAA 1 cut(s) 230
TscAI CASTG 1 cut(s) 436
TseI GCWGC 3 cut(s) 110, 410, 455
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 436
XspI CTAG 2 cut(s) 155, 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.