Rh4CG058000

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001823
Physical Location & Seq
Reverse (-)
11227660 .. 11228451
792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG058000.1

Sequence Viewer

Length: 792 bp
ATGGCTATTCTCCAACAACGTCGCAAGATCCCCTCCTTGTCTTTAGCCTCCACCGCCGTCCACCACCTCCCACTTCTCTCTCTACAAACCATCCCTCGCCCCGCCGTCACCACCACCTTCACCTCTTCCTCTTCCACAGACCCCATCTCCGCCTTAGACCTAGAGCGACTCCAAGTCTTGGGCCACGGCAGCGGCGGCACCGTCTACAAGGTCCTTCACAAGCCCACTTCCACCCCTTACGCTCTCAAACAACTCCACTCTCCGGCTGCTGACTCCTCCTCCGATAACAACACACTTCAGAGTGAACTCGACATCCTCCGCCGCACAACCGGCTCTCCCTACGTCGTACACTGCCACGCCATCTTCGAGAAGCCCGCCGGAGACGTGTCGGTTCTCATGGAGTACATGGAGTTGGGTTCTCTTGAAACCTTGCTCAAGTCCCAAGGTCCCTTCTCCGAAGAAAAGCTTGCCCCCATCGCACGCCAAGTCCTCGAAGGCCTCGACTACCTTCACAAGACTCACAAGATTGTTCACCGCGACATCAAGCCCGGGAATCTTTTAATGAACAACAAGAAGGAGGTCAAGATCGCGGACTTTGGTTGCAGTAGTGATGAGTCTTGCAGTTCTTCCGTGGGGACTTGTGCTTATATGAGCCCGGAGCGGTTTGATCCGGAGAGATACGTTGGAAGTCACAATGGCTATACCAGTGATATATGGAGCTTTGGGGTGACTCTAATGGAACTCTACATGGGTTTCTTCCCCTTGCTCCCCAAGGGTCAGAGACCGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

263

Amino Acids

28.93

Weight (kDa)

6.59

Isoelectric Point (pI)

55.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 55 - 256 3e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 55 - 250 1e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 197
AccB7I CCANNNNNTGG 1 cut(s) 178
AccBSI CCGCTC 1 cut(s) 661
AccI GTMKAC 1 cut(s) 204
AccII CGCG 2 cut(s) 537, 590
AccIII TCCGGA 1 cut(s) 670
AclWI GGATC 2 cut(s) 22, 662
AcuI CTGAAG 1 cut(s) 281
AfaI GTAC 2 cut(s) 348, 404
AfiI CCNNNNNNNGG 2 cut(s) 178, 262
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 1 cut(s) 425
AhdI GACNNNNNGTC 1 cut(s) 173
AjiI CACGTC 1 cut(s) 385
AloI GAACNNNNNNTCC 2 cut(s) 297, 329
AluBI AGCT 2 cut(s) 466, 720
AluI AGCT 2 cut(s) 466, 720
Alw26I GTCTC 2 cut(s) 375, 775
AlwI GGATC 2 cut(s) 22, 662
Ama87I CYCGRG 1 cut(s) 548
Aor13HI TCCGGA 1 cut(s) 670
AoxI GGCC 2 cut(s) 181, 496
ApeKI GCWGC 2 cut(s) 189, 266
AspS9I GGNCC 3 cut(s) 181, 211, 446
AsuC2I CCSGG 3 cut(s) 549, 550, 656
AsuHPI GGTGA 4 cut(s) 100, 112, 524, 739
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 2 cut(s) 211, 446
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 656
BbvI GCAGC 2 cut(s) 201, 253
BccI CCATC 4 cut(s) 98, 152, 368, 482
BceAI ACGGC 3 cut(s) 41, 89, 202
BcnI CCSGG 3 cut(s) 549, 550, 656
BcoDI GTCTC 2 cut(s) 375, 775
BfaI CTAG 1 cut(s) 161
BisI GCNGC 5 cut(s) 190, 193, 196, 267, 322
BlsI GCNGC 5 cut(s) 191, 194, 197, 268, 323
Bme1390I CCNGG 3 cut(s) 549, 550, 656
Bme18I GGWCC 2 cut(s) 211, 446
BmeRI GACNNNNNGTC 1 cut(s) 173
BmeT110I CYCGRG 1 cut(s) 548
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 3 cut(s) 181, 211, 446
BmiI GGNNCC 2 cut(s) 199, 448
BmrFI CCNGG 3 cut(s) 549, 550, 656
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 3 cut(s) 549, 550, 656
BsaI GGTCTC 1 cut(s) 775
BsaJI CCNNGG 5 cut(s) 184, 442, 548, 630, 771
BsaWI WCCGGW 2 cut(s) 670, 784
BsaXI ACNNNNNCTCC 6 cut(s) 131, 161, 263, 293, 319, 349
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 262
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 1 cut(s) 705
BseAI TCCGGA 1 cut(s) 670
BseDI CCNNGG 5 cut(s) 184, 442, 548, 630, 771
BseGI GGATG 2 cut(s) 90, 312
BseLI CCNNNNNNNGG 2 cut(s) 178, 262
BseNI ACTGG 1 cut(s) 705
BseRI GAGGAG 2 cut(s) 265, 268
BseXI GCAGC 2 cut(s) 201, 253
Bsh1236I CGCG 2 cut(s) 537, 590
BshFI GGCC 2 cut(s) 183, 498
BshNI GGYRCC 1 cut(s) 197
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 7 cut(s) 263, 330, 378, 549, 656, 671, 785
BslFI GGGAC 3 cut(s) 424, 432, 649
BslI CCNNNNNNNGG 2 cut(s) 178, 262
BsmAI GTCTC 2 cut(s) 375, 775
BsmBI CGTCTC 1 cut(s) 375
BsmFI GGGAC 3 cut(s) 424, 432, 649
BsnI GGCC 2 cut(s) 183, 498
Bso31I GGTCTC 1 cut(s) 775
BsoBI CYCGRG 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 656
Bsp13I TCCGGA 1 cut(s) 670
Bsp143I GATC 3 cut(s) 27, 585, 667
BspANI GGCC 2 cut(s) 183, 498
BspEI TCCGGA 1 cut(s) 670
BspFNI CGCG 2 cut(s) 537, 590
BspLI GGNNCC 2 cut(s) 199, 448
BspPI GGATC 2 cut(s) 22, 662
BspT107I GGYRCC 1 cut(s) 197
BspTNI GGTCTC 1 cut(s) 775
BsrBI CCGCTC 1 cut(s) 661
BsrFI RCCGGY 1 cut(s) 329
BsrI ACTGG 1 cut(s) 705
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 5 cut(s) 184, 442, 548, 630, 771
BssMI GATC 3 cut(s) 27, 585, 667
BssT1I CCWWGG 2 cut(s) 442, 771
Bst4CI ACNGT 1 cut(s) 202
Bst6I CTCTTC 2 cut(s) 130, 136
BstC8I GCNNGC 3 cut(s) 375, 468, 481
BstDEI CTNAG 1 cut(s) 154
BstDSI CCRYGG 2 cut(s) 184, 630
BstF5I GGATG 2 cut(s) 90, 312
BstFNI CGCG 2 cut(s) 537, 590
BstKTI GATC 3 cut(s) 30, 588, 670
BstMAI GTCTC 2 cut(s) 375, 775
BstMBI GATC 3 cut(s) 27, 585, 667
BstMWI GCNNNNNNNGC 5 cut(s) 53, 189, 195, 330, 476
BstSCI CCNGG 3 cut(s) 547, 548, 654
BstUI CGCG 2 cut(s) 537, 590
BstV1I GCAGC 2 cut(s) 201, 253
BstX2I RGATCY 1 cut(s) 27
BstYI RGATCY 1 cut(s) 27
BsuRI GGCC 2 cut(s) 183, 498
BtgI CCRYGG 2 cut(s) 184, 630
BtgZI GCGATG 1 cut(s) 460
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 2 cut(s) 90, 312
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 349, 712
Cac8I GCNNGC 3 cut(s) 375, 468, 481
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 3 cut(s) 181, 211, 446
Cfr9I CCCGGG 1 cut(s) 548
Csp6I GTAC 2 cut(s) 347, 403
CviAII CATG 3 cut(s) 397, 406, 748
CviQI GTAC 2 cut(s) 347, 403
DdeI CTNAG 1 cut(s) 154
DpnI GATC 3 cut(s) 29, 587, 669
DpnII GATC 3 cut(s) 27, 585, 667
DriI GACNNNNNGTC 1 cut(s) 173
Eam1104I CTCTTC 2 cut(s) 130, 136
Eam1105I GACNNNNNGTC 1 cut(s) 173
EarI CTCTTC 2 cut(s) 130, 136
EciI GGCGGA 2 cut(s) 139, 308
Eco130I CCWWGG 2 cut(s) 442, 771
Eco147I AGGCCT 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 656
Eco31I GGTCTC 1 cut(s) 775
Eco47I GGWCC 2 cut(s) 211, 446
Eco57I CTGAAG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 548
EcoO109I RGGNCCY 2 cut(s) 211, 446
EcoT14I CCWWGG 2 cut(s) 442, 771
EcoT38I GRGCYC 1 cut(s) 656
ErhI CCWWGG 2 cut(s) 442, 771
Esp3I CGTCTC 1 cut(s) 375
FaeI CATG 3 cut(s) 400, 409, 751
FaiI YATR 8 cut(s) 398, 407, 648, 650, 702, 713, 715, 749
FalI AAGNNNNNCTT 2 cut(s) 450, 482
FaqI GGGAC 3 cut(s) 424, 432, 649
FatI CATG 3 cut(s) 396, 405, 747
FauI CCCGC 2 cut(s) 109, 382
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 5 cut(s) 190, 193, 196, 267, 322
FokI GGATG 2 cut(s) 77, 299
FriOI GRGCYC 1 cut(s) 656
Fsp4HI GCNGC 5 cut(s) 190, 193, 196, 267, 322
FspBI CTAG 1 cut(s) 161
GluI GCNGC 5 cut(s) 190, 193, 196, 267, 322
HaeIII GGCC 2 cut(s) 183, 498
HapII CCGG 7 cut(s) 263, 330, 378, 549, 656, 671, 785
Hin1II CATG 3 cut(s) 400, 409, 751
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 6 cut(s) 168, 272, 517, 553, 614, 730
HpaII CCGG 7 cut(s) 263, 330, 378, 549, 656, 671, 785
HphI GGTGA 4 cut(s) 100, 112, 524, 739
Hpy166II GTNNAC 5 cut(s) 61, 205, 305, 349, 532
Hpy188I TCNGA 4 cut(s) 283, 300, 457, 780
Hpy188III TCNNGA 4 cut(s) 367, 422, 583, 671
Hpy8I GTNNAC 5 cut(s) 61, 205, 305, 349, 532
Hpy99I CGWCG 2 cut(s) 24, 347
HpyAV CCTTC 6 cut(s) 127, 224, 460, 488, 518, 568
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 4 cut(s) 19, 342, 384, 681
HpyCH4V TGCA 2 cut(s) 603, 621
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 189, 195, 330, 476
HpyF3I CTNAG 1 cut(s) 154
HpySE526I ACGT 4 cut(s) 19, 342, 384, 681
Hsp92II CATG 3 cut(s) 400, 409, 751
Kpn2I TCCGGA 1 cut(s) 670
Kzo9I GATC 3 cut(s) 27, 585, 667
LmnI GCTCC 3 cut(s) 658, 717, 771
LpnPI CCDG 7 cut(s) 276, 343, 391, 562, 669, 684, 718
Lsp1109I GCAGC 2 cut(s) 201, 253
MaeI CTAG 1 cut(s) 161
MaeII ACGT 4 cut(s) 19, 342, 384, 681
MaeIII GTNAC 3 cut(s) 106, 689, 727
MalI GATC 3 cut(s) 29, 587, 669
MbiI CCGCTC 1 cut(s) 661
MboI GATC 3 cut(s) 27, 585, 667
MboII GAAGA 6 cut(s) 117, 123, 355, 470, 618, 748
MflI RGATCY 1 cut(s) 27
MhlI GDGCHC 1 cut(s) 656
MlyI GAGTC 5 cut(s) 162, 266, 511, 623, 724
MmeI TCCRAC 2 cut(s) 37, 664
MroI TCCGGA 1 cut(s) 670
MseI TTAA 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 192
MspI CCGG 7 cut(s) 263, 330, 378, 549, 656, 671, 785
MspR9I CCNGG 3 cut(s) 549, 550, 656
MvnI CGCG 2 cut(s) 537, 590
MwoI GCNNNNNNNGC 5 cut(s) 53, 189, 195, 330, 476
NciI CCSGG 3 cut(s) 549, 550, 656
NdeII GATC 3 cut(s) 27, 585, 667
NlaIII CATG 3 cut(s) 400, 409, 751
NlaIV GGNNCC 2 cut(s) 199, 448
NmuCI GTSAC 3 cut(s) 106, 689, 727
PceI AGGCCT 1 cut(s) 498
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 498
PfeI GAWTC 1 cut(s) 553
PflMI CCANNNNNTGG 1 cut(s) 178
PkrI GCNGC 5 cut(s) 191, 194, 197, 268, 323
PleI GAGTC 5 cut(s) 162, 266, 511, 622, 724
PpsI GAGTC 5 cut(s) 162, 266, 511, 622, 724
PpuMI RGGWCCY 2 cut(s) 211, 446
Psp5II RGGWCCY 2 cut(s) 211, 446
PspN4I GGNNCC 2 cut(s) 199, 448
PspPI GGNCC 3 cut(s) 181, 211, 446
PspPPI RGGWCCY 2 cut(s) 211, 446
PsuI RGATCY 1 cut(s) 27
RsaI GTAC 2 cut(s) 348, 404
RsaNI GTAC 2 cut(s) 347, 403
SaqAI TTAA 1 cut(s) 560
SatI GCNGC 5 cut(s) 190, 193, 196, 267, 322
Sau3AI GATC 3 cut(s) 27, 585, 667
Sau96I GGNCC 3 cut(s) 181, 211, 446
SchI GAGTC 5 cut(s) 162, 266, 511, 623, 724
ScrFI CCNGG 3 cut(s) 549, 550, 656
SduI GDGCHC 1 cut(s) 656
SinI GGWCC 2 cut(s) 211, 446
SmaI CCCGGG 1 cut(s) 550
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
SseBI AGGCCT 1 cut(s) 498
SspMI CTAG 1 cut(s) 161
StuI AGGCCT 1 cut(s) 498
StyD4I CCNGG 3 cut(s) 547, 548, 654
StyI CCWWGG 2 cut(s) 442, 771
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 4 cut(s) 22, 345, 387, 684
TaqI TCGA 4 cut(s) 309, 366, 492, 501
TatI WGTACW 1 cut(s) 402
TauI GCSGC 3 cut(s) 195, 198, 324
TfiI GAWTC 1 cut(s) 553
Tru1I TTAA 1 cut(s) 560
Tru9I TTAA 1 cut(s) 560
TscAI CASTG 2 cut(s) 356, 712
TseFI GTSAC 3 cut(s) 106, 689, 727
TseI GCWGC 2 cut(s) 189, 266
Tsp45I GTSAC 3 cut(s) 106, 689, 727
TspDTI ATGAA 1 cut(s) 578
TspGWI ACGGA 1 cut(s) 619
TspMI CCCGGG 1 cut(s) 548
TspRI CASTG 2 cut(s) 356, 712
Van91I CCANNNNNTGG 1 cut(s) 178
VpaK11BI GGWCC 2 cut(s) 211, 446
XmaI CCCGGG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.