Rroxscaffold_5G00339630

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001823
Physical Location & Seq
Reverse (-)
8063212 .. 8078972
15761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00339630.1

Sequence Viewer

Length: 1239 bp
ATGGCAAAAGCTGCGAAGAAATTCTCTTCCTTTGGAGTGAACGGTGATTGCACGTTTCTTGAATTGTTCCACGTCCCCTTGATTGCCACGTATGTGGGAGACCTTGCTTCCTTGCTTAATTGGCCCACTATAGGACTTAATTATTTGAGGAGGATGGTCTGCGTCTCAAGCCACTTCGATCGGCCAAGTTTGGCTGATTTTGGTGGCCGAAAACTAATTAATGAAGCGTTGACCGAAATCGGCACCGTGGTGTTCTCGGCCTTGGGTCTTCATTGTCGGCCAAGACGCCGTGATCTACCACCATGGGGAGGTTGGGTACGGGCAGAAGAGTCCAACGGTGCCCCCCTCATGTTCCCGGGCGGCCGGAGGAGGAAGTTGCGAGAGTTTTCCGTGGAAAAGACCAACAATCGGTCATTTGAGATTGCTTTCAATAGGATAAATATGTATAGGAGTAGCATCACTCTGGTATCCCACTCACAACTTCAGATCGATCGATCGATCAACATGGCTCTTCTCCAACAACGTCGCAAGATCCCCTCCTTGTCTTTAGCCTCCACCGCCGTCCACCACCTCCCACTTCTCTCTCTACAAACCATCCCTCGCCCCGCTGTCACCACCACCTTCACCTCTTCCTCTTCCACAGACCCCATCTCCGCCTTAGACCTAGAGCGACTCCAATTCTTGGGCCACGGCAGCGGCGGCACCGTCTACAAGGTCCTTCACAAGCCCACTTCCACCCCTTACGCTCTCAAACAACTCCACTCGCCGGCTGCTGACTCCTCCTCCGATAACAACACACTTCAGAGTGAACTCGACATCCTCCGCCGCACAACCGGCTCTCCCTACGTCGTACACTGCCACGCCATCTTCAAGAAGCCCTCCGGAGACGTGTCGGTTCTCATGGAGTACATGGAGTTGGGTTCTCTTGAAACCTTGCTCAAGTCCCAAGGTCCCTTCTCCGAAGAAAAGCTTGCCCCCATTGCACGCCAAGTCCTCGAAGGCCTCGACTACCTTCACAAGACTCACAAGATTGTTCACCGCGACATCAAGCCCGGGAATCTTTTAATGAACAACAAGAAGGAGATCAAGATCGCGGACTTTGGTTGCAGTGGTGATGAGTCTTGCAGTTCTTCCGTGGGGACTTGTGCTTATATGAGCCCGGAGCGGTTTGATCCGGCGAGATACGGTACGGTGGAAGTCACAATGGCTATACCAGTGATATATGGAGCTTTGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

412

Amino Acids

45.5

Weight (kDa)

9.23

Isoelectric Point (pI)

55.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 223 - 396 7.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 225 - 388 3.3e-23 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 242, 338, 701
AccB7I CCANNNNNTGG 1 cut(s) 682
AccBSI CCGCTC 1 cut(s) 1165
AccI GTMKAC 1 cut(s) 708
AccII CGCG 2 cut(s) 1041, 1094
AccIII TCCGGA 1 cut(s) 881
AclWI GGATC 2 cut(s) 526, 1166
AcoI YGGCCR 4 cut(s) 182, 205, 278, 361
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 2 cut(s) 467, 785
AcyI GRCGYC 1 cut(s) 286
AfaI GTAC 4 cut(s) 318, 852, 908, 1189
AfiI CCNNNNNNNGG 6 cut(s) 131, 305, 308, 408, 682, 766
AflIII ACRYGT 1 cut(s) 888
AgsI TTSAA 4 cut(s) 62, 430, 871, 929
AjiI CACGTC 2 cut(s) 73, 889
AleI CACNNNNGTG 2 cut(s) 92, 248
AloI GAACNNNNNNTCC 2 cut(s) 801, 833
AluBI AGCT 3 cut(s) 11, 970, 1229
AluI AGCT 3 cut(s) 11, 970, 1229
Alw26I GTCTC 3 cut(s) 93, 169, 879
AlwI GGATC 2 cut(s) 526, 1166
Ama87I CYCGRG 2 cut(s) 355, 1052
Aor13HI TCCGGA 1 cut(s) 881
AoxI GGCC 8 cut(s) 122, 182, 205, 258, 278, 361, 685, 1000
ApeKI GCWGC 3 cut(s) 11, 693, 770
ApoI RAATTY 1 cut(s) 20
AseI ATTAAT 1 cut(s) 219
Asp700I GAANNNNTTC 1 cut(s) 20
AspS9I GGNCC 4 cut(s) 123, 685, 715, 950
AsuC2I CCSGG 5 cut(s) 356, 357, 1053, 1054, 1160
AsuHPI GGTGA 5 cut(s) 56, 604, 616, 1028, 1124
AvaI CYCGRG 2 cut(s) 355, 1052
AvaII GGWCC 2 cut(s) 715, 950
BaeGI GKGCMC 1 cut(s) 343
BaeI ACNNNNGTAYC 2 cut(s) 450, 483
BanI GGYRCC 3 cut(s) 242, 338, 701
BanII GRGCYC 1 cut(s) 1160
BbsI GAAGAC 1 cut(s) 260
BbvI GCAGC 2 cut(s) 705, 757
BccI CCATC 4 cut(s) 148, 602, 656, 872
BceAI ACGGC 3 cut(s) 273, 545, 706
BciVI GTATCC 1 cut(s) 478
BcnI CCSGG 5 cut(s) 356, 357, 1053, 1054, 1160
BcoDI GTCTC 3 cut(s) 93, 169, 879
BfaI CTAG 1 cut(s) 665
BfmI CTRYAG 1 cut(s) 129
BfuI GTATCC 1 cut(s) 478
BisI GCNGC 7 cut(s) 12, 361, 694, 697, 700, 771, 826
BlsI GCNGC 7 cut(s) 13, 362, 695, 698, 701, 772, 827
Bme1390I CCNGG 5 cut(s) 356, 357, 1053, 1054, 1160
Bme18I GGWCC 2 cut(s) 715, 950
BmeT110I CYCGRG 2 cut(s) 355, 1052
BmgBI CACGTC 2 cut(s) 73, 889
BmgT120I GGNCC 4 cut(s) 123, 685, 715, 950
BmiI GGNNCC 4 cut(s) 244, 340, 703, 952
BmrFI CCNGG 5 cut(s) 356, 357, 1053, 1054, 1160
BmsI GCATC 1 cut(s) 465
BpiI GAAGAC 1 cut(s) 260
BpuEI CTTGAG 2 cut(s) 151, 923
BpuMI CCSGG 5 cut(s) 356, 357, 1053, 1054, 1160
Bsa29I ATCGAT 3 cut(s) 489, 493, 497
BsaAI YACGTR 1 cut(s) 90
BsaBI GATNNNNATC 1 cut(s) 1088
BsaHI GRCGYC 1 cut(s) 286
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 9 cut(s) 246, 261, 302, 355, 390, 688, 946, 1052, 1134
BsaWI WCCGGW 1 cut(s) 881
BsaXI ACNNNNNCTCC 8 cut(s) 300, 330, 635, 665, 767, 797, 823, 853
Bsc4I CCNNNNNNNGG 6 cut(s) 131, 305, 308, 408, 682, 766
Bse118I RCCGGY 2 cut(s) 766, 833
Bse1I ACTGG 1 cut(s) 1214
Bse3DI GCAATG 1 cut(s) 978
Bse8I GATNNNNATC 1 cut(s) 1088
BseAI TCCGGA 1 cut(s) 881
BseCI ATCGAT 3 cut(s) 489, 493, 497
BseDI CCNNGG 9 cut(s) 246, 261, 302, 355, 390, 688, 946, 1052, 1134
BseGI GGATG 3 cut(s) 159, 594, 816
BseJI GATNNNNATC 1 cut(s) 1088
BseLI CCNNNNNNNGG 6 cut(s) 131, 305, 308, 408, 682, 766
BseMI GCAATG 1 cut(s) 978
BseNI ACTGG 1 cut(s) 1214
BseRI GAGGAG 4 cut(s) 163, 382, 769, 772
BseSI GKGCMC 1 cut(s) 343
BseX3I CGGCCG 1 cut(s) 361
BseXI GCAGC 2 cut(s) 705, 757
Bsh1236I CGCG 2 cut(s) 1041, 1094
Bsh1285I CGRYCG 4 cut(s) 181, 364, 493, 497
BshFI GGCC 8 cut(s) 124, 184, 207, 260, 280, 363, 687, 1002
BshNI GGYRCC 3 cut(s) 242, 338, 701
BshVI ATCGAT 3 cut(s) 489, 493, 497
BsiEI CGRYCG 4 cut(s) 181, 364, 493, 497
BsiHKCI CYCGRG 2 cut(s) 355, 1052
BsiSI CCGG 8 cut(s) 356, 364, 767, 834, 882, 1053, 1160, 1175
BslFI GGGAC 4 cut(s) 59, 928, 936, 1153
BslI CCNNNNNNNGG 6 cut(s) 131, 305, 308, 408, 682, 766
BsmAI GTCTC 3 cut(s) 93, 169, 879
BsmBI CGTCTC 2 cut(s) 169, 879
BsmFI GGGAC 4 cut(s) 59, 928, 936, 1153
BsnI GGCC 8 cut(s) 124, 184, 207, 260, 280, 363, 687, 1002
Bso31I GGTCTC 1 cut(s) 93
BsoBI CYCGRG 2 cut(s) 355, 1052
Bsp1286I GDGCHC 2 cut(s) 343, 1160
Bsp13I TCCGGA 1 cut(s) 881
Bsp19I CCATGG 1 cut(s) 302
BspANI GGCC 8 cut(s) 124, 184, 207, 260, 280, 363, 687, 1002
BspDI ATCGAT 3 cut(s) 489, 493, 497
BspEI TCCGGA 1 cut(s) 881
BspFNI CGCG 2 cut(s) 1041, 1094
BspLI GGNNCC 4 cut(s) 244, 340, 703, 952
BspPI GGATC 2 cut(s) 526, 1166
BspQI GCTCTTC 1 cut(s) 516
BspT107I GGYRCC 3 cut(s) 242, 338, 701
BspTNI GGTCTC 1 cut(s) 93
BsrBI CCGCTC 1 cut(s) 1165
BsrDI GCAATG 1 cut(s) 978
BsrFI RCCGGY 2 cut(s) 766, 833
BsrI ACTGG 1 cut(s) 1214
BssAI RCCGGY 2 cut(s) 766, 833
BssECI CCNNGG 9 cut(s) 246, 261, 302, 355, 390, 688, 946, 1052, 1134
BssNI GRCGYC 1 cut(s) 286
BssT1I CCWWGG 3 cut(s) 261, 302, 946
Bst4CI ACNGT 6 cut(s) 44, 247, 338, 706, 1187, 1192
Bst6I CTCTTC 5 cut(s) 31, 321, 516, 634, 640
BstACI GRCGYC 1 cut(s) 286
BstAPI GCANNNNNTGC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 90
BstC8I GCNNGC 3 cut(s) 768, 972, 985
BstDEI CTNAG 1 cut(s) 658
BstDSI CCRYGG 5 cut(s) 246, 302, 390, 688, 1134
BstF5I GGATG 3 cut(s) 159, 594, 816
BstFNI CGCG 2 cut(s) 1041, 1094
BstMAI GTCTC 3 cut(s) 93, 169, 879
BstMCI CGRYCG 4 cut(s) 181, 364, 493, 497
BstMWI GCNNNNNNNGC 8 cut(s) 11, 121, 168, 557, 693, 699, 834, 980
BstSCI CCNGG 5 cut(s) 354, 355, 1051, 1052, 1158
BstSFI CTRYAG 1 cut(s) 129
BstSLI GKGCMC 1 cut(s) 343
BstUI CGCG 2 cut(s) 1041, 1094
BstV1I GCAGC 2 cut(s) 705, 757
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 531
BstXI CCANNNNNNTGG 1 cut(s) 94
BstYI RGATCY 1 cut(s) 531
BstZI CGGCCG 1 cut(s) 361
Bsu15I ATCGAT 3 cut(s) 489, 493, 497
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 8 cut(s) 124, 184, 207, 260, 280, 363, 687, 1002
BsuTUI ATCGAT 3 cut(s) 489, 493, 497
BtgI CCRYGG 5 cut(s) 246, 302, 390, 688, 1134
BtrI CACGTC 2 cut(s) 73, 889
BtsCI GGATG 3 cut(s) 159, 594, 816
BtsI GCAGTG 2 cut(s) 853, 1114
BtsIMutI CAGTG 3 cut(s) 853, 1114, 1221
Cac8I GCNNGC 3 cut(s) 768, 972, 985
Cfr10I RCCGGY 2 cut(s) 766, 833
Cfr13I GGNCC 4 cut(s) 123, 685, 715, 950
Cfr9I CCCGGG 2 cut(s) 355, 1052
ClaI ATCGAT 3 cut(s) 489, 493, 497
CseI GACGC 2 cut(s) 151, 294
Csp6I GTAC 4 cut(s) 317, 851, 907, 1188
CviAII CATG 5 cut(s) 303, 349, 505, 901, 910
CviQI GTAC 4 cut(s) 317, 851, 907, 1188
DdeI CTNAG 1 cut(s) 658
EaeI YGGCCR 4 cut(s) 182, 205, 278, 361
EagI CGGCCG 1 cut(s) 361
Eam1104I CTCTTC 5 cut(s) 31, 321, 516, 634, 640
EarI CTCTTC 5 cut(s) 31, 321, 516, 634, 640
EciI GGCGGA 2 cut(s) 643, 812
EclXI CGGCCG 1 cut(s) 361
Eco130I CCWWGG 3 cut(s) 261, 302, 946
Eco147I AGGCCT 1 cut(s) 1002
Eco24I GRGCYC 1 cut(s) 1160
Eco31I GGTCTC 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 715, 950
Eco52I CGGCCG 1 cut(s) 361
Eco57I CTGAAG 2 cut(s) 467, 785
Eco88I CYCGRG 2 cut(s) 355, 1052
EcoO109I RGGNCCY 2 cut(s) 715, 950
EcoT14I CCWWGG 3 cut(s) 261, 302, 946
EcoT38I GRGCYC 1 cut(s) 1160
ErhI CCWWGG 3 cut(s) 261, 302, 946
Esp3I CGTCTC 2 cut(s) 169, 879
FaeI CATG 5 cut(s) 306, 352, 508, 904, 913
FalI AAGNNNNNCTT 2 cut(s) 954, 986
FaqI GGGAC 4 cut(s) 59, 928, 936, 1153
FatI CATG 5 cut(s) 302, 348, 504, 900, 909
FauI CCCGC 1 cut(s) 613
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 7 cut(s) 12, 361, 694, 697, 700, 771, 826
FokI GGATG 3 cut(s) 166, 581, 803
FriOI GRGCYC 1 cut(s) 1160
Fsp4HI GCNGC 7 cut(s) 12, 361, 694, 697, 700, 771, 826
FspBI CTAG 1 cut(s) 665
GluI GCNGC 7 cut(s) 12, 361, 694, 697, 700, 771, 826
HaeIII GGCC 8 cut(s) 124, 184, 207, 260, 280, 363, 687, 1002
HapII CCGG 8 cut(s) 356, 364, 767, 834, 882, 1053, 1160, 1175
HgaI GACGC 2 cut(s) 151, 294
Hin1I GRCGYC 1 cut(s) 286
Hin1II CATG 5 cut(s) 306, 352, 508, 904, 913
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HindIII AAGCTT 1 cut(s) 968
HinfI GANTC 6 cut(s) 329, 672, 776, 1021, 1057, 1118
HpaII CCGG 8 cut(s) 356, 364, 767, 834, 882, 1053, 1160, 1175
HphI GGTGA 5 cut(s) 56, 604, 616, 1028, 1124
Hpy166II GTNNAC 7 cut(s) 40, 231, 565, 709, 809, 853, 1036
Hpy188I TCNGA 4 cut(s) 486, 787, 804, 961
Hpy188III TCNNGA 5 cut(s) 59, 871, 882, 926, 1087
Hpy8I GTNNAC 7 cut(s) 40, 231, 565, 709, 809, 853, 1036
Hpy99I CGWCG 2 cut(s) 528, 851
HpyAV CCTTC 6 cut(s) 631, 728, 964, 992, 1022, 1072
HpyCH4III ACNGT 6 cut(s) 44, 247, 338, 706, 1187, 1192
HpyCH4IV ACGT 6 cut(s) 53, 72, 89, 523, 846, 888
HpyCH4V TGCA 4 cut(s) 51, 983, 1107, 1125
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 121, 168, 557, 693, 699, 834, 980
HpyF3I CTNAG 1 cut(s) 658
HpySE526I ACGT 6 cut(s) 53, 72, 89, 523, 846, 888
Hsp92I GRCGYC 1 cut(s) 286
Hsp92II CATG 5 cut(s) 306, 352, 508, 904, 913
Kpn2I TCCGGA 1 cut(s) 881
KroI GCCGGC 1 cut(s) 766
KroNI GCCGGC 1 cut(s) 768
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 2 cut(s) 1162, 1226
Lsp1109I GCAGC 2 cut(s) 705, 757
LweI GCATC 1 cut(s) 465
MaeI CTAG 1 cut(s) 665
MaeII ACGT 6 cut(s) 53, 72, 89, 523, 846, 888
MaeIII GTNAC 2 cut(s) 610, 1198
MbiI CCGCTC 1 cut(s) 1165
MflI RGATCY 1 cut(s) 531
MhlI GDGCHC 2 cut(s) 343, 1160
MluCI AATT 6 cut(s) 20, 62, 118, 139, 216, 677
MlyI GAGTC 5 cut(s) 338, 666, 770, 1015, 1127
MmeI TCCRAC 2 cut(s) 357, 541
MroI TCCGGA 1 cut(s) 881
MroNI GCCGGC 1 cut(s) 766
MroXI GAANNNNTTC 1 cut(s) 20
MseI TTAA 4 cut(s) 117, 138, 219, 1064
MslI CAYNNNNRTG 2 cut(s) 92, 248
MspA1I CMGCKG 2 cut(s) 608, 696
MspI CCGG 8 cut(s) 356, 364, 767, 834, 882, 1053, 1160, 1175
MspR9I CCNGG 5 cut(s) 356, 357, 1053, 1054, 1160
MvnI CGCG 2 cut(s) 1041, 1094
MwoI GCNNNNNNNGC 8 cut(s) 11, 121, 168, 557, 693, 699, 834, 980
NaeI GCCGGC 1 cut(s) 768
NciI CCSGG 5 cut(s) 356, 357, 1053, 1054, 1160
NcoI CCATGG 1 cut(s) 302
NgoMIV GCCGGC 1 cut(s) 766
NlaIII CATG 5 cut(s) 306, 352, 508, 904, 913
NlaIV GGNNCC 4 cut(s) 244, 340, 703, 952
NmeAIII GCCGAG 1 cut(s) 236
NmuCI GTSAC 2 cut(s) 610, 1198
OliI CACNNNNGTG 2 cut(s) 92, 248
PceI AGGCCT 1 cut(s) 1002
PciSI GCTCTTC 1 cut(s) 516
PcsI WCGNNNNNNNCGW 1 cut(s) 1002
PdiI GCCGGC 1 cut(s) 768
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 1 cut(s) 1057
PflMI CCANNNNNTGG 1 cut(s) 682
PkrI GCNGC 7 cut(s) 13, 362, 695, 698, 701, 772, 827
Ple19I CGATCG 3 cut(s) 181, 493, 497
PleI GAGTC 5 cut(s) 337, 666, 770, 1015, 1126
PpsI GAGTC 5 cut(s) 337, 666, 770, 1015, 1126
Ppu21I YACGTR 1 cut(s) 90
PpuMI RGGWCCY 2 cut(s) 715, 950
PshBI ATTAAT 1 cut(s) 219
Psp5II RGGWCCY 2 cut(s) 715, 950
PspN4I GGNNCC 4 cut(s) 244, 340, 703, 952
PspPI GGNCC 4 cut(s) 123, 685, 715, 950
PspPPI RGGWCCY 2 cut(s) 715, 950
PsuI RGATCY 1 cut(s) 531
PvuI CGATCG 3 cut(s) 181, 493, 497
RsaI GTAC 4 cut(s) 318, 852, 908, 1189
RsaNI GTAC 4 cut(s) 317, 851, 907, 1188
RseI CAYNNNNRTG 2 cut(s) 92, 248
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 4 cut(s) 117, 138, 219, 1064
SatI GCNGC 7 cut(s) 12, 361, 694, 697, 700, 771, 826
Sau96I GGNCC 4 cut(s) 123, 685, 715, 950
SchI GAGTC 5 cut(s) 338, 666, 770, 1015, 1127
ScrFI CCNGG 5 cut(s) 356, 357, 1053, 1054, 1160
SduI GDGCHC 2 cut(s) 343, 1160
SfaNI GCATC 1 cut(s) 465
SfcI CTRYAG 1 cut(s) 129
SinI GGWCC 2 cut(s) 715, 950
SmaI CCCGGG 2 cut(s) 357, 1054
SmiMI CAYNNNNRTG 2 cut(s) 92, 248
SmlI CTYRAG 2 cut(s) 166, 938
SmoI CTYRAG 2 cut(s) 166, 938
Sse9I AATT 6 cut(s) 20, 62, 118, 139, 216, 677
SseBI AGGCCT 1 cut(s) 1002
SspMI CTAG 1 cut(s) 665
StuI AGGCCT 1 cut(s) 1002
StyD4I CCNGG 5 cut(s) 354, 355, 1051, 1052, 1158
StyI CCWWGG 3 cut(s) 261, 302, 946
TaaI ACNGT 6 cut(s) 44, 247, 338, 706, 1187, 1192
TaiI ACGT 6 cut(s) 56, 75, 92, 526, 849, 891
TaqI TCGA 7 cut(s) 177, 489, 493, 497, 813, 996, 1005
TaqII GACCGA 2 cut(s) 248, 399
TasI AATT 6 cut(s) 20, 62, 118, 139, 216, 677
TatI WGTACW 1 cut(s) 906
TauI GCSGC 4 cut(s) 363, 699, 702, 828
TfiI GAWTC 1 cut(s) 1057
Tru1I TTAA 4 cut(s) 117, 138, 219, 1064
Tru9I TTAA 4 cut(s) 117, 138, 219, 1064
TscAI CASTG 3 cut(s) 860, 1114, 1221
TseFI GTSAC 2 cut(s) 610, 1198
TseI GCWGC 3 cut(s) 11, 693, 770
Tsp45I GTSAC 2 cut(s) 610, 1198
TspDTI ATGAA 3 cut(s) 237, 260, 1082
TspGWI ACGGA 2 cut(s) 379, 1123
TspMI CCCGGG 2 cut(s) 355, 1052
TspRI CASTG 3 cut(s) 860, 1114, 1221
Van91I CCANNNNNTGG 1 cut(s) 682
VpaK11BI GGWCC 2 cut(s) 715, 950
VspI ATTAAT 1 cut(s) 219
XapI RAATTY 1 cut(s) 20
XcmI CCANNNNNNNNNTGG 1 cut(s) 309
XmaI CCCGGG 2 cut(s) 355, 1052
XmiI GTMKAC 1 cut(s) 708
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.