RLG00000003933

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001823
Physical Location & Seq
Reverse (-)
54426061 .. 54427205
1145 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003933

Sequence Viewer

Length: 453 bp
ATGGAGTACATGGACTTGGGTTCTCTTGAAACCTTGCTCAAGTCCCAAAGTCCCTTATCCGAAGAAAAGCTTGCCCCCGTCGCAAGCCAAGTCCTCGAAGGCCTCGACTGCCTTCACAATACTCACAAGATTGTTCACGGCGACATCAAGCCCGGGCATCTTTTAATGAATAGCAAGATGGAGAGGCGCCATGCTTGGCGGAGGGTGCGTCTGAAGACTTTTGGGAGCTTTCTTGAGTGTTGCATGCACGAGGAGGCCTCCAAGAGGTGGACGGCGGCTCAACTTTTGACCCACCCGTTCGTCTGCAAGGCTCAATCCGATGAGTGCAAGCCAAACTTGTTGAACAAGAAAAGAAAACTTCCAAATGGGAAGAACAAGAAAATCGAGCCAGACAAAGCCCATCATTGGCCGTATACAAATGGGCAAGGGAGGAAGATGGGGAAGCTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

151

Amino Acids

17.22

Weight (kDa)

9.54

Isoelectric Point (pI)

65.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 75 5.4e-11 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 186
AccB7I CCANNNNNTGG 1 cut(s) 267
AccI GTMKAC 1 cut(s) 413
AciI CCGC 2 cut(s) 199, 275
AcoI YGGCCR 1 cut(s) 407
AcuI CTGAAG 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 187
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 3 cut(s) 264, 267, 405
AgsI TTSAA 2 cut(s) 29, 343
AluBI AGCT 3 cut(s) 70, 228, 445
AluI AGCT 3 cut(s) 70, 228, 445
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 3 cut(s) 100, 255, 407
AspLEI GCGC 1 cut(s) 189
AsuC2I CCSGG 2 cut(s) 153, 154
AvaI CYCGRG 1 cut(s) 152
BanI GGYRCC 1 cut(s) 186
BauI CACGAG 1 cut(s) 248
BbsI GAAGAC 1 cut(s) 221
BccI CCATC 3 cut(s) 172, 408, 430
BceAI ACGGC 3 cut(s) 154, 288, 394
BcnI CCSGG 2 cut(s) 153, 154
BfoI RGCGCY 1 cut(s) 190
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
Bme1390I CCNGG 2 cut(s) 153, 154
BmeT110I CYCGRG 1 cut(s) 152
BmiI GGNNCC 1 cut(s) 188
BmrFI CCNGG 2 cut(s) 153, 154
BmsI GCATC 1 cut(s) 166
BpiI GAAGAC 1 cut(s) 221
BplI GAGNNNNNCTC 2 cut(s) 242, 274
BpuEI CTTGAG 2 cut(s) 23, 254
BpuMI CCSGG 2 cut(s) 153, 154
BsaHI GRCGYC 1 cut(s) 187
BsaJI CCNNGG 1 cut(s) 152
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bsc4I CCNNNNNNNGG 3 cut(s) 264, 267, 405
BseDI CCNNGG 1 cut(s) 152
BseLI CCNNNNNNNGG 3 cut(s) 264, 267, 405
BseRI GAGGAG 1 cut(s) 266
BshFI GGCC 3 cut(s) 102, 257, 409
BshNI GGYRCC 1 cut(s) 186
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 1 cut(s) 153
BslFI GGGAC 2 cut(s) 28, 36
BslI CCNNNNNNNGG 3 cut(s) 264, 267, 405
BsmFI GGGAC 2 cut(s) 28, 36
BsnI GGCC 3 cut(s) 102, 257, 409
BsoBI CYCGRG 1 cut(s) 152
BspACI CCGC 2 cut(s) 199, 275
BspANI GGCC 3 cut(s) 102, 257, 409
BspLI GGNNCC 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 186
BssECI CCNNGG 1 cut(s) 152
BssNAI GTATAC 1 cut(s) 414
BssNI GRCGYC 1 cut(s) 187
BssSI CACGAG 1 cut(s) 248
Bst1107I GTATAC 1 cut(s) 414
Bst2BI CACGAG 1 cut(s) 248
BstACI GRCGYC 1 cut(s) 187
BstC8I GCNNGC 4 cut(s) 72, 85, 245, 329
BstENI CCTNNNNNAGG 1 cut(s) 262
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 1 cut(s) 189
BstMWI GCNNNNNNNGC 3 cut(s) 80, 108, 205
BstNSI RCATGY 1 cut(s) 247
BstSCI CCNGG 2 cut(s) 151, 152
BstV2I GAAGAC 1 cut(s) 221
BstZ17I GTATAC 1 cut(s) 414
BsuRI GGCC 3 cut(s) 102, 257, 409
Cac8I GCNNGC 4 cut(s) 72, 85, 245, 329
CfoI GCGC 1 cut(s) 189
Cfr9I CCCGGG 1 cut(s) 152
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 7
CviAII CATG 3 cut(s) 10, 191, 244
CviQI GTAC 1 cut(s) 7
DinI GGCGCC 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 407
EciI GGCGGA 1 cut(s) 214
Eco147I AGGCCT 2 cut(s) 102, 257
Eco57I CTGAAG 1 cut(s) 233
Eco88I CYCGRG 1 cut(s) 152
EcoNI CCTNNNNNAGG 1 cut(s) 262
EgeI GGCGCC 1 cut(s) 188
EheI GGCGCC 1 cut(s) 188
FaeI CATG 3 cut(s) 13, 194, 247
FaiI YATR 4 cut(s) 11, 192, 245, 414
FalI AAGNNNNNCTT 4 cut(s) 54, 86, 320, 352
FaqI GGGAC 2 cut(s) 28, 36
FatI CATG 3 cut(s) 9, 190, 243
FblI GTMKAC 1 cut(s) 413
Fnu4HI GCNGC 1 cut(s) 276
Fsp4HI GCNGC 1 cut(s) 276
GlaI GCGC 1 cut(s) 188
GluI GCNGC 1 cut(s) 276
HaeII RGCGCY 1 cut(s) 190
HaeIII GGCC 3 cut(s) 102, 257, 409
HapII CCGG 1 cut(s) 153
HgaI GACGC 1 cut(s) 197
HhaI GCGC 1 cut(s) 189
Hin1I GRCGYC 1 cut(s) 187
Hin1II CATG 3 cut(s) 13, 194, 247
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HindIII AAGCTT 1 cut(s) 68
HpaII CCGG 1 cut(s) 153
Hpy166II GTNNAC 3 cut(s) 136, 270, 414
Hpy188I TCNGA 3 cut(s) 61, 213, 319
Hpy188III TCNNGA 2 cut(s) 26, 233
Hpy8I GTNNAC 3 cut(s) 136, 270, 414
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 2 cut(s) 92, 122
HpyCH4V TGCA 4 cut(s) 243, 247, 306, 327
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 108, 205
Hsp92I GRCGYC 1 cut(s) 187
Hsp92II CATG 3 cut(s) 13, 194, 247
HspAI GCGC 1 cut(s) 187
KasI GGCGCC 1 cut(s) 186
LmnI GCTCC 1 cut(s) 225
LpnPI CCDG 2 cut(s) 166, 402
LweI GCATC 1 cut(s) 166
MboII GAAGA 4 cut(s) 74, 226, 382, 445
Mly113I GGCGCC 1 cut(s) 187
MnlI CCTC 9 cut(s) 104, 113, 177, 195, 244, 247, 258, 268, 423
MseI TTAA 1 cut(s) 164
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 2 cut(s) 153, 154
MwoI GCNNNNNNNGC 3 cut(s) 80, 108, 205
NarI GGCGCC 1 cut(s) 187
NciI CCSGG 2 cut(s) 153, 154
NlaIII CATG 3 cut(s) 13, 194, 247
NlaIV GGNNCC 1 cut(s) 188
NspI RCATGY 1 cut(s) 247
PaeI GCATGC 1 cut(s) 247
PceI AGGCCT 2 cut(s) 102, 257
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PflMI CCANNNNNTGG 1 cut(s) 267
PkrI GCNGC 1 cut(s) 277
PluTI GGCGCC 1 cut(s) 190
PspN4I GGNNCC 1 cut(s) 188
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SaqAI TTAA 1 cut(s) 164
SatI GCNGC 1 cut(s) 276
ScrFI CCNGG 2 cut(s) 153, 154
SetI ASST 5 cut(s) 35, 72, 230, 269, 447
SfaNI GCATC 1 cut(s) 166
SfoI GGCGCC 1 cut(s) 188
SmaI CCCGGG 1 cut(s) 154
SmlI CTYRAG 2 cut(s) 38, 233
SmoI CTYRAG 2 cut(s) 38, 233
SphI GCATGC 1 cut(s) 247
SrfI GCCCGGGC 1 cut(s) 154
SseBI AGGCCT 2 cut(s) 102, 257
SsiI CCGC 2 cut(s) 199, 275
SspDI GGCGCC 1 cut(s) 186
StuI AGGCCT 2 cut(s) 102, 257
StyD4I CCNGG 2 cut(s) 151, 152
TaqI TCGA 3 cut(s) 96, 105, 384
TatI WGTACW 1 cut(s) 6
TauI GCSGC 1 cut(s) 278
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TspDTI ATGAA 1 cut(s) 182
TspMI CCCGGG 1 cut(s) 152
Van91I CCANNNNNTGG 1 cut(s) 267
XagI CCTNNNNNAGG 1 cut(s) 262
XceI RCATGY 1 cut(s) 247
XmaI CCCGGG 1 cut(s) 152
XmiI GTMKAC 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.