Rorug07G0040300

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001823
Physical Location & Seq
Forward (+)
2830074 .. 2832074
2001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0040300.1

Sequence Viewer

Length: 657 bp
ATGGCAGAGGCAACCGTCGCTGAAATCGCCGAGACCAACACCAACACCGCGGCGGCCGAGGACATGGACCTCGAAACCGCCCCGGCCCAAAACCCAGACGAGGCCCAGAACGGCGACGCAAACTCTAAGCGGGCCAGGGAGGAAGAAGAAGAGAGCGGCGGCGACGCGAAGAAGACGAAGGTGGTGGAGGAGAAGTCAGCGGAAGAGCAACGGTTGGAGAAGTTAGGGGAAGGAGAGAGCGAGGCGGGTCGGGTCATCTTGGGTCCGAAGAGTTTCGGGTCGGCCGTTGAGATGTTCGACTATTTCTATAAGCTACTCCATTACTGGCCAACTGATCTTAATATTAATAAGTATGAATATCTGGTTTTGCTGGACTTGCTCAAGAAGGGTCACTCTGAGCCTGATAAAAAGATTGGTGGAGGAATCCAGGCTTTCCAAGTTCGGTTCCACCCATTGTATAGAAGTAGGTGCTACTTCCTTGTTAGAGAAGATGGAGCCAGTGATGATTTTAGTTTCAGGAAGTGTGTGGATCATATACTTCCCTTGCCAGAGAACATGAAAGCACACGCTGATGCCAACAAAGCCTTAGGCGGTGGCAAGGGTGGTGGGGGGAGAGGAAGCTGGCGTGGCCGTGGAAGGGGCAGGTCAAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

218

Amino Acids

24.1

Weight (kDa)

5.52

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 104 - 176 2.3e-25 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 633
AccBSI CCGCTC 1 cut(s) 156
AccII CGCG 2 cut(s) 50, 167
AclWI GGATC 1 cut(s) 537
AcoI YGGCCR 4 cut(s) 54, 282, 326, 628
AfiI CCNNNNNNNGG 2 cut(s) 100, 637
AjnI CCWGG 2 cut(s) 134, 426
AluBI AGCT 2 cut(s) 313, 621
AluI AGCT 2 cut(s) 313, 621
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 1 cut(s) 537
AoxI GGCC 7 cut(s) 54, 84, 102, 132, 282, 326, 628
AseI ATTAAT 1 cut(s) 345
Asp700I GAANNNNTTC 1 cut(s) 272
AspS9I GGNCC 5 cut(s) 67, 85, 103, 132, 263
AsuC2I CCSGG 1 cut(s) 83
AvaII GGWCC 2 cut(s) 67, 263
AxyI CCTNAGG 1 cut(s) 586
BalI TGGCCA 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 179
BccI CCATC 1 cut(s) 485
BceAI ACGGC 3 cut(s) 127, 269, 615
BciT130I CCWGG 2 cut(s) 136, 428
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 26
BfuAI ACCTGC 1 cut(s) 633
BisI GCNGC 4 cut(s) 51, 54, 157, 160
BlsI GCNGC 4 cut(s) 52, 55, 158, 161
Bme1390I CCNGG 3 cut(s) 83, 136, 428
Bme18I GGWCC 2 cut(s) 67, 263
BmgT120I GGNCC 5 cut(s) 67, 85, 103, 132, 263
BmiI GGNNCC 3 cut(s) 264, 446, 496
BmrFI CCNGG 3 cut(s) 83, 136, 428
BmsI GCATC 1 cut(s) 562
BpiI GAAGAC 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 83
BsaI GGTCTC 1 cut(s) 26
BsaJI CCNNGG 5 cut(s) 48, 57, 81, 135, 631
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 637
Bse1I ACTGG 2 cut(s) 329, 498
Bse21I CCTNAGG 1 cut(s) 586
BseBI CCWGG 2 cut(s) 136, 428
BseDI CCNNGG 5 cut(s) 48, 57, 81, 135, 631
BseLI CCNNNNNNNGG 2 cut(s) 100, 637
BseMII CTCAG 1 cut(s) 387
BseNI ACTGG 2 cut(s) 329, 498
BseRI GAGGAG 1 cut(s) 203
BseX3I CGGCCG 2 cut(s) 54, 282
Bsh1236I CGCG 2 cut(s) 50, 167
Bsh1285I CGRYCG 2 cut(s) 57, 285
BshFI GGCC 7 cut(s) 56, 86, 104, 134, 284, 328, 630
BsiEI CGRYCG 2 cut(s) 57, 285
BsiSI CCGG 1 cut(s) 83
BslI CCNNNNNNNGG 2 cut(s) 100, 637
BsmAI GTCTC 1 cut(s) 26
BsnI GGCC 7 cut(s) 56, 86, 104, 134, 284, 328, 630
Bso31I GGTCTC 1 cut(s) 26
Bsp143I GATC 2 cut(s) 334, 529
BspANI GGCC 7 cut(s) 56, 86, 104, 134, 284, 328, 630
BspCNI CTCAG 1 cut(s) 388
BspFNI CGCG 2 cut(s) 50, 167
BspLI GGNNCC 3 cut(s) 264, 446, 496
BspMI ACCTGC 1 cut(s) 633
BspPI GGATC 1 cut(s) 537
BspQI GCTCTTC 1 cut(s) 198
BspTNI GGTCTC 1 cut(s) 26
BsrBI CCGCTC 1 cut(s) 156
BsrI ACTGG 2 cut(s) 329, 498
BssECI CCNNGG 5 cut(s) 48, 57, 81, 135, 631
BssMI GATC 2 cut(s) 334, 529
Bst2UI CCWGG 2 cut(s) 136, 428
Bst4CI ACNGT 2 cut(s) 16, 213
Bst6I CTCTTC 3 cut(s) 144, 198, 263
BstC8I GCNNGC 2 cut(s) 132, 623
BstDEI CTNAG 3 cut(s) 126, 396, 586
BstDSI CCRYGG 2 cut(s) 48, 631
BstFNI CGCG 2 cut(s) 50, 167
BstKTI GATC 2 cut(s) 337, 532
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 2 cut(s) 334, 529
BstMCI CGRYCG 2 cut(s) 57, 285
BstMWI GCNNNNNNNGC 5 cut(s) 17, 26, 376, 581, 627
BstNI CCWGG 2 cut(s) 136, 428
BstSCI CCNGG 3 cut(s) 81, 134, 426
BstUI CGCG 2 cut(s) 50, 167
BstV2I GAAGAC 1 cut(s) 179
BstZI CGGCCG 2 cut(s) 54, 282
Bsu36I CCTNAGG 1 cut(s) 586
BsuRI GGCC 7 cut(s) 56, 86, 104, 134, 284, 328, 630
BtgI CCRYGG 2 cut(s) 48, 631
BtsIMutI CAGTG 1 cut(s) 505
BveI ACCTGC 1 cut(s) 633
Cac8I GCNNGC 2 cut(s) 132, 623
Cfr13I GGNCC 5 cut(s) 67, 85, 103, 132, 263
Cfr42I CCGCGG 1 cut(s) 51
CseI GACGC 2 cut(s) 125, 173
CspCI CAANNNNNGTGG 2 cut(s) 586, 621
CviAII CATG 2 cut(s) 64, 556
DdeI CTNAG 3 cut(s) 126, 396, 586
DpnI GATC 2 cut(s) 336, 531
DpnII GATC 2 cut(s) 334, 529
EaeI YGGCCR 4 cut(s) 54, 282, 326, 628
EagI CGGCCG 2 cut(s) 54, 282
Eam1104I CTCTTC 3 cut(s) 144, 198, 263
EarI CTCTTC 3 cut(s) 144, 198, 263
EclXI CGGCCG 2 cut(s) 54, 282
Eco31I GGTCTC 1 cut(s) 26
Eco47I GGWCC 2 cut(s) 67, 263
Eco52I CGGCCG 2 cut(s) 54, 282
Eco81I CCTNAGG 1 cut(s) 586
EcoRII CCWGG 2 cut(s) 134, 426
FaeI CATG 2 cut(s) 67, 559
FaiI YATR 7 cut(s) 65, 309, 354, 459, 534, 536, 557
FatI CATG 2 cut(s) 63, 555
FauI CCCGC 2 cut(s) 123, 238
Fnu4HI GCNGC 4 cut(s) 51, 54, 157, 160
Fsp4HI GCNGC 4 cut(s) 51, 54, 157, 160
GluI GCNGC 4 cut(s) 51, 54, 157, 160
HaeIII GGCC 7 cut(s) 56, 86, 104, 134, 284, 328, 630
HapII CCGG 1 cut(s) 83
HgaI GACGC 2 cut(s) 125, 173
Hin1II CATG 2 cut(s) 67, 559
HinfI GANTC 1 cut(s) 423
HpaII CCGG 1 cut(s) 83
Hpy188I TCNGA 2 cut(s) 267, 397
Hpy188III TCNNGA 3 cut(s) 382, 517, 648
Hpy99I CGWCG 3 cut(s) 20, 119, 167
HpyAV CCTTC 4 cut(s) 172, 224, 379, 630
HpyCH4III ACNGT 2 cut(s) 16, 213
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 26, 376, 581, 627
HpyF3I CTNAG 3 cut(s) 126, 396, 586
Hsp92II CATG 2 cut(s) 67, 559
KspI CCGCGG 1 cut(s) 51
Kzo9I GATC 2 cut(s) 334, 529
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 1 cut(s) 494
LweI GCATC 1 cut(s) 562
MaeIII GTNAC 1 cut(s) 389
MalI GATC 2 cut(s) 336, 531
MbiI CCGCTC 1 cut(s) 156
MboI GATC 2 cut(s) 334, 529
MboII GAAGA 8 cut(s) 155, 158, 161, 181, 184, 215, 280, 500
MlsI TGGCCA 1 cut(s) 328
MluNI TGGCCA 1 cut(s) 328
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 8 cut(s) 52, 80, 94, 133, 181, 235, 413, 608
Mox20I TGGCCA 1 cut(s) 328
MroXI GAANNNNTTC 1 cut(s) 272
MscI TGGCCA 1 cut(s) 328
MseI TTAA 2 cut(s) 339, 345
MslI CAYNNNNRTG 1 cut(s) 570
Msp20I TGGCCA 1 cut(s) 328
MspA1I CMGCKG 2 cut(s) 50, 200
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 3 cut(s) 83, 136, 428
MvaI CCWGG 2 cut(s) 136, 428
MvnI CGCG 2 cut(s) 50, 167
MwoI GCNNNNNNNGC 5 cut(s) 17, 26, 376, 581, 627
NciI CCSGG 1 cut(s) 83
NdeII GATC 2 cut(s) 334, 529
NlaIII CATG 2 cut(s) 67, 559
NlaIV GGNNCC 3 cut(s) 264, 446, 496
NmeAIII GCCGAG 2 cut(s) 55, 82
NmuCI GTSAC 1 cut(s) 389
PciSI GCTCTTC 1 cut(s) 198
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 1 cut(s) 423
PkrI GCNGC 4 cut(s) 52, 55, 158, 161
PshBI ATTAAT 1 cut(s) 345
Psp6I CCWGG 2 cut(s) 134, 426
PspGI CCWGG 2 cut(s) 134, 426
PspN4I GGNNCC 3 cut(s) 264, 446, 496
PspPI GGNCC 5 cut(s) 67, 85, 103, 132, 263
RseI CAYNNNNRTG 1 cut(s) 570
SacII CCGCGG 1 cut(s) 51
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 2 cut(s) 339, 345
SatI GCNGC 4 cut(s) 51, 54, 157, 160
Sau3AI GATC 2 cut(s) 334, 529
Sau96I GGNCC 5 cut(s) 67, 85, 103, 132, 263
ScrFI CCNGG 3 cut(s) 83, 136, 428
SetI ASST 6 cut(s) 72, 183, 315, 470, 623, 647
SfaNI GCATC 1 cut(s) 562
Sfr303I CCGCGG 1 cut(s) 51
SgrBI CCGCGG 1 cut(s) 51
SinI GGWCC 2 cut(s) 67, 263
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SspI AATATT 1 cut(s) 343
StyD4I CCNGG 3 cut(s) 81, 134, 426
TaaI ACNGT 2 cut(s) 16, 213
TaqI TCGA 2 cut(s) 72, 297
TauI GCSGC 4 cut(s) 53, 56, 159, 162
TfiI GAWTC 1 cut(s) 423
Tru1I TTAA 2 cut(s) 339, 345
Tru9I TTAA 2 cut(s) 339, 345
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 1 cut(s) 389
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 2 cut(s) 369, 572
TspRI CASTG 1 cut(s) 505
VpaK11BI GGWCC 2 cut(s) 67, 263
VspI ATTAAT 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.