Prupe.5G236900_v2.0.a1

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001823
Physical Location & Seq
Reverse (-)
17911322 .. 17912829
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G236900.1

Sequence Viewer

Length: 975 bp
ATGGCTCTCATACGAGAACGCCGACAGCTTAATCTCCGCCTACCCTTGCCCGAACCCTCCGAGTGCCGCCCACGTTTCGCCCTTCCTCTTCCTCCCACAGCCGCCGCCACCACCGCCCTCACAAACAACTCTTCCTCCGCCGCTATCTCCGCCGCCGATCTCGACACACTCCAAGTCCTCGGCCACGGTAACGGCGGCACCGTCTACAAGGTACGCCACAAGCGCACTTCCACCACTTACGCTCTCAAACTCGTCTACGGCGACTCCGACCCCACCGTCCGCCGTCAGATCTTCCGCGAGATGGAGATCCTCCGCCGCACGGACTCTCCCTACGTCGTCCGCTGCCACGCAATCTTTGAGAAGCCGTCGGGAGATATCGGGATCGTCATGGAGTACATGGACTCCGGCACCCTCGAGACCTTGCTTAAAGCCCACGGCACCTTCTCCGAGCCAAAGCTTGCCCACGTCGCCCGCCAAGTTCTCAACGGCCTCAACTACCTCCACACCCACAAGATCATCCACCGCGACATTAAACCCACCAATCTTTTGGTCAACAGCAACATGGAAGTGAAGATCGCCGACTTTGGCGTGAGCAAGATCATGTGCCGGACCTTGGACGCCTGCAATTCCTACGTGGGTACTTGCGCTTACATGAGCCCGGAAAGATTCGACCCGGACACTTACGGTGGCAATTACAACGGCTACGCCAGTGATATATGGAGCCTGGGGCTGACCCTGATGGAGCTTTACATGGGTCACTTTCCGTTCTTGCCTCCGGGTCAGAGACCCGACTGGGCCACCCTTATGTGCGCCATATGTTTCGGAGAGCCGCCGAGCTTGCCCGATGGGGTGTCGGAGGAGTTCAGGAGTTTCATGGAGTGTTGCTTGCAGAAGGAGTCCGGAAAACGGTGGACGGCCGCTCAGCTTTTGACCCACCCGTTCGTCTGTAAAGATCCGAGTGTATCCGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000165 GO:0000187 GO:0001101 GO:0001932 GO:0001934 GO:0002229 GO:0002239 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007568 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009403 GO:0009404 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009700 GO:0009719 GO:0009723 GO:0009725 GO:0009751 GO:0009755 GO:0009791 GO:0009814 GO:0009838 GO:0009862 GO:0009863 GO:0009873 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010120 GO:0010150 GO:0010227 GO:0010229 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010817 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019207 GO:0019209 GO:0019219 GO:0019220 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019887 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030234 GO:0030295 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0034641 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043449 GO:0043450 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044272 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0046217 GO:0046483 GO:0046677 GO:0046777 GO:0048366 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071369 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0090567 GO:0090693 GO:0098542 GO:0098772 GO:0099402 GO:0140096 GO:1900673 GO:1900674 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

325

Amino Acids

36.09

Weight (kDa)

7.58

Isoelectric Point (pI)

50.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
AccB1I GGYRCC 3 cut(s) 197, 407, 437
AccBSI CCGCTC 1 cut(s) 920
AccI GTMKAC 2 cut(s) 204, 255
AccII CGCG 2 cut(s) 297, 525
AccIII TCCGGA 1 cut(s) 899
AclWI GGATC 3 cut(s) 301, 389, 947
AcoI YGGCCR 2 cut(s) 181, 915
AcyI GRCGYC 1 cut(s) 618
AfaI GTAC 3 cut(s) 213, 395, 640
AfiI CCNNNNNNNGG 4 cut(s) 301, 319, 613, 906
AjiI CACGTC 1 cut(s) 466
AjnI CCWGG 1 cut(s) 723
AluBI AGCT 5 cut(s) 28, 457, 745, 837, 925
AluI AGCT 5 cut(s) 28, 457, 745, 837, 925
Alw26I GTCTC 2 cut(s) 410, 778
AlwI GGATC 3 cut(s) 301, 389, 947
Ama87I CYCGRG 1 cut(s) 413
Aor13HI TCCGGA 1 cut(s) 899
AoxI GGCC 4 cut(s) 181, 487, 795, 915
ApeKI GCWGC 1 cut(s) 342
AspLEI GCGC 3 cut(s) 225, 647, 812
AspS9I GGNCC 2 cut(s) 609, 795
AsuC2I CCSGG 3 cut(s) 659, 674, 777
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 1 cut(s) 609
BanI GGYRCC 3 cut(s) 197, 407, 437
BanII GRGCYC 1 cut(s) 659
BbvI GCAGC 1 cut(s) 329
BccI CCATC 3 cut(s) 295, 733, 839
BceAI ACGGC 8 cut(s) 208, 267, 274, 349, 451, 502, 715, 930
BciT130I CCWGG 1 cut(s) 725
BciVI GTATCC 1 cut(s) 973
BcnI CCSGG 3 cut(s) 659, 674, 777
BcoDI GTCTC 2 cut(s) 410, 778
BfuI GTATCC 1 cut(s) 973
BglII AGATCT 1 cut(s) 288
BlpI GCTNAGC 1 cut(s) 921
Bme1390I CCNGG 4 cut(s) 659, 674, 725, 777
Bme18I GGWCC 1 cut(s) 609
BmeT110I CYCGRG 1 cut(s) 413
BmgBI CACGTC 1 cut(s) 466
BmgT120I GGNCC 2 cut(s) 609, 795
BmiI GGNNCC 4 cut(s) 199, 409, 439, 722
BmrFI CCNGG 4 cut(s) 659, 674, 725, 777
BmrI ACTGGG 1 cut(s) 802
BmuI ACTGGG 1 cut(s) 802
Bpu1102I GCTNAGC 1 cut(s) 921
BpuMI CCSGG 3 cut(s) 659, 674, 777
BsaAI YACGTR 1 cut(s) 634
BsaBI GATNNNNATC 1 cut(s) 305
BsaHI GRCGYC 1 cut(s) 618
BsaI GGTCTC 2 cut(s) 410, 778
BsaJI CCNNGG 5 cut(s) 178, 184, 433, 612, 724
BsaWI WCCGGW 1 cut(s) 899
BsaXI ACNNNNNCTCC 8 cut(s) 119, 149, 248, 278, 310, 340, 386, 416
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 319, 613, 906
Bse1I ACTGG 2 cut(s) 708, 797
Bse8I GATNNNNATC 1 cut(s) 305
BseAI TCCGGA 1 cut(s) 899
BseBI CCWGG 1 cut(s) 725
BseDI CCNNGG 5 cut(s) 178, 184, 433, 612, 724
BseGI GGATG 1 cut(s) 516
BseJI GATNNNNATC 1 cut(s) 305
BseLI CCNNNNNNNGG 4 cut(s) 301, 319, 613, 906
BseMII CTCAG 1 cut(s) 935
BseNI ACTGG 2 cut(s) 708, 797
BseRI GAGGAG 1 cut(s) 872
BseX3I CGGCCG 1 cut(s) 915
BseXI GCAGC 1 cut(s) 329
Bsh1236I CGCG 2 cut(s) 297, 525
Bsh1285I CGRYCG 1 cut(s) 918
BshFI GGCC 4 cut(s) 183, 489, 797, 917
BshNI GGYRCC 3 cut(s) 197, 407, 437
BsiEI CGRYCG 1 cut(s) 918
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 6 cut(s) 405, 607, 659, 674, 776, 900
BslI CCNNNNNNNGG 4 cut(s) 301, 319, 613, 906
BsmAI GTCTC 2 cut(s) 410, 778
BsnI GGCC 4 cut(s) 183, 489, 797, 917
Bso31I GGTCTC 2 cut(s) 410, 778
BsoBI CYCGRG 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 659
Bsp13I TCCGGA 1 cut(s) 899
Bsp143I GATC 8 cut(s) 157, 288, 306, 381, 513, 573, 597, 952
Bsp1720I GCTNAGC 1 cut(s) 921
BspANI GGCC 4 cut(s) 183, 489, 797, 917
BspCNI CTCAG 1 cut(s) 934
BspEI TCCGGA 1 cut(s) 899
BspFNI CGCG 2 cut(s) 297, 525
BspLI GGNNCC 4 cut(s) 199, 409, 439, 722
BspPI GGATC 3 cut(s) 301, 389, 947
BspT107I GGYRCC 3 cut(s) 197, 407, 437
BspTNI GGTCTC 2 cut(s) 410, 778
BsrBI CCGCTC 1 cut(s) 920
BsrI ACTGG 2 cut(s) 708, 797
BssECI CCNNGG 5 cut(s) 178, 184, 433, 612, 724
BssMI GATC 8 cut(s) 157, 288, 306, 381, 513, 573, 597, 952
BssNI GRCGYC 1 cut(s) 618
BssT1I CCWWGG 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 725
Bst4CI ACNGT 5 cut(s) 188, 202, 277, 686, 909
Bst6I CTCTTC 2 cut(s) 93, 136
BstACI GRCGYC 1 cut(s) 618
BstBAI YACGTR 1 cut(s) 634
BstC8I GCNNGC 5 cut(s) 459, 472, 622, 839, 887
BstDEI CTNAG 1 cut(s) 921
BstDSI CCRYGG 2 cut(s) 184, 433
BstF5I GGATG 1 cut(s) 516
BstFNI CGCG 2 cut(s) 297, 525
BstHHI GCGC 3 cut(s) 225, 647, 812
BstKTI GATC 8 cut(s) 160, 291, 309, 384, 516, 576, 600, 955
BstMAI GTCTC 2 cut(s) 410, 778
BstMBI GATC 8 cut(s) 157, 288, 306, 381, 513, 573, 597, 952
BstMCI CGRYCG 1 cut(s) 918
BstMWI GCNNNNNNNGC 5 cut(s) 113, 149, 222, 467, 838
BstNI CCWGG 1 cut(s) 725
BstSCI CCNGG 4 cut(s) 657, 672, 723, 775
BstUI CGCG 2 cut(s) 297, 525
BstV1I GCAGC 1 cut(s) 329
BstX2I RGATCY 3 cut(s) 288, 306, 952
BstXI CCANNNNNNTGG 1 cut(s) 547
BstYI RGATCY 3 cut(s) 288, 306, 952
BstZI CGGCCG 1 cut(s) 915
BsuI GTATCC 1 cut(s) 973
BsuRI GGCC 4 cut(s) 183, 489, 797, 917
BtgI CCRYGG 2 cut(s) 184, 433
BtrI CACGTC 1 cut(s) 466
BtsCI GGATG 1 cut(s) 516
BtsIMutI CAGTG 1 cut(s) 715
Cac8I GCNNGC 5 cut(s) 459, 472, 622, 839, 887
CfoI GCGC 3 cut(s) 225, 647, 812
Cfr13I GGNCC 2 cut(s) 609, 795
CseI GACGC 1 cut(s) 626
Csp6I GTAC 3 cut(s) 212, 394, 639
CviAII CATG 7 cut(s) 388, 397, 562, 601, 652, 751, 874
CviQI GTAC 3 cut(s) 212, 394, 639
DdeI CTNAG 1 cut(s) 921
DpnI GATC 8 cut(s) 159, 290, 308, 383, 515, 575, 599, 954
DpnII GATC 8 cut(s) 157, 288, 306, 381, 513, 573, 597, 952
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 2 cut(s) 181, 915
EagI CGGCCG 1 cut(s) 915
Eam1104I CTCTTC 2 cut(s) 93, 136
EarI CTCTTC 2 cut(s) 93, 136
EciI GGCGGA 5 cut(s) 26, 127, 139, 269, 302
EclXI CGGCCG 1 cut(s) 915
Eco130I CCWWGG 1 cut(s) 612
Eco24I GRGCYC 1 cut(s) 659
Eco31I GGTCTC 2 cut(s) 410, 778
Eco32I GATATC 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 609
Eco52I CGGCCG 1 cut(s) 915
Eco88I CYCGRG 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 723
EcoRV GATATC 1 cut(s) 376
EcoT14I CCWWGG 1 cut(s) 612
EcoT38I GRGCYC 1 cut(s) 659
ErhI CCWWGG 1 cut(s) 612
FaeI CATG 7 cut(s) 391, 400, 565, 604, 655, 754, 877
FatI CATG 7 cut(s) 387, 396, 561, 600, 651, 750, 873
FauI CCCGC 1 cut(s) 479
FauNDI CATATG 1 cut(s) 815
FblI GTMKAC 2 cut(s) 204, 255
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 659
GlaI GCGC 3 cut(s) 224, 646, 811
HaeIII GGCC 4 cut(s) 183, 489, 797, 917
HapII CCGG 6 cut(s) 405, 607, 659, 674, 776, 900
HgaI GACGC 1 cut(s) 626
HhaI GCGC 3 cut(s) 225, 647, 812
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 7 cut(s) 391, 400, 565, 604, 655, 754, 877
Hin6I GCGC 3 cut(s) 223, 645, 810
HinP1I GCGC 3 cut(s) 223, 645, 810
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 5 cut(s) 263, 323, 401, 666, 896
HpaII CCGG 6 cut(s) 405, 607, 659, 674, 776, 900
Hpy166II GTNNAC 4 cut(s) 205, 256, 553, 912
Hpy188I TCNGA 8 cut(s) 61, 268, 288, 448, 783, 824, 856, 957
Hpy188III TCNNGA 7 cut(s) 161, 369, 379, 415, 865, 900, 972
Hpy8I GTNNAC 4 cut(s) 205, 256, 553, 912
Hpy99I CGWCG 3 cut(s) 338, 370, 470
HpyAV CCTTC 3 cut(s) 92, 451, 886
HpyCH4III ACNGT 5 cut(s) 188, 202, 277, 686, 909
HpyCH4IV ACGT 4 cut(s) 73, 333, 465, 633
HpyCH4V TGCA 2 cut(s) 624, 889
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 149, 222, 467, 838
HpyF3I CTNAG 1 cut(s) 921
HpySE526I ACGT 4 cut(s) 73, 333, 465, 633
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 7 cut(s) 391, 400, 565, 604, 655, 754, 877
HspAI GCGC 3 cut(s) 223, 645, 810
Kpn2I TCCGGA 1 cut(s) 899
Kzo9I GATC 8 cut(s) 157, 288, 306, 381, 513, 573, 597, 952
LmnI GCTCC 2 cut(s) 720, 742
Lsp1109I GCAGC 1 cut(s) 329
MaeII ACGT 4 cut(s) 73, 333, 465, 633
MaeIII GTNAC 2 cut(s) 188, 755
MalI GATC 8 cut(s) 159, 290, 308, 383, 515, 575, 599, 954
MbiI CCGCTC 1 cut(s) 920
MboI GATC 8 cut(s) 157, 288, 306, 381, 513, 573, 597, 952
MboII GAAGA 4 cut(s) 80, 123, 283, 583
MflI RGATCY 3 cut(s) 288, 306, 952
MhlI GDGCHC 1 cut(s) 659
MluCI AATT 2 cut(s) 625, 691
MlyI GAGTC 4 cut(s) 257, 317, 395, 905
MmeI TCCRAC 2 cut(s) 291, 834
MroI TCCGGA 1 cut(s) 899
MseI TTAA 3 cut(s) 30, 426, 531
MslI CAYNNNNRTG 2 cut(s) 566, 803
MspA1I CMGCKG 1 cut(s) 342
MspI CCGG 6 cut(s) 405, 607, 659, 674, 776, 900
MspR9I CCNGG 4 cut(s) 659, 674, 725, 777
MvaI CCWGG 1 cut(s) 725
MvnI CGCG 2 cut(s) 297, 525
MwoI GCNNNNNNNGC 5 cut(s) 113, 149, 222, 467, 838
NciI CCSGG 3 cut(s) 659, 674, 777
NdeI CATATG 1 cut(s) 815
NdeII GATC 8 cut(s) 157, 288, 306, 381, 513, 573, 597, 952
NlaIII CATG 7 cut(s) 391, 400, 565, 604, 655, 754, 877
NlaIV GGNNCC 4 cut(s) 199, 409, 439, 722
NmeAIII GCCGAG 2 cut(s) 159, 858
NmuCI GTSAC 1 cut(s) 755
PaeR7I CTCGAG 1 cut(s) 413
PcsI WCGNNNNNNNCGW 4 cut(s) 19, 198, 258, 264
PfeI GAWTC 1 cut(s) 666
PleI GAGTC 4 cut(s) 257, 317, 395, 904
PpsI GAGTC 4 cut(s) 257, 317, 395, 904
Ppu21I YACGTR 1 cut(s) 634
Psp6I CCWGG 1 cut(s) 723
PspGI CCWGG 1 cut(s) 723
PspN4I GGNNCC 4 cut(s) 199, 409, 439, 722
PspPI GGNCC 2 cut(s) 609, 795
PsuI RGATCY 3 cut(s) 288, 306, 952
RsaI GTAC 3 cut(s) 213, 395, 640
RsaNI GTAC 3 cut(s) 212, 394, 639
RseI CAYNNNNRTG 2 cut(s) 566, 803
SaqAI TTAA 3 cut(s) 30, 426, 531
Sau3AI GATC 8 cut(s) 157, 288, 306, 381, 513, 573, 597, 952
Sau96I GGNCC 2 cut(s) 609, 795
SchI GAGTC 4 cut(s) 257, 317, 395, 905
ScrFI CCNGG 4 cut(s) 659, 674, 725, 777
SduI GDGCHC 1 cut(s) 659
Sfr274I CTCGAG 1 cut(s) 413
SinI GGWCC 1 cut(s) 609
SlaI CTCGAG 1 cut(s) 413
SmiMI CAYNNNNRTG 2 cut(s) 566, 803
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 2 cut(s) 625, 691
StyD4I CCNGG 4 cut(s) 657, 672, 723, 775
StyI CCWWGG 1 cut(s) 612
TaaI ACNGT 5 cut(s) 188, 202, 277, 686, 909
TaiI ACGT 4 cut(s) 76, 336, 468, 636
TaqI TCGA 3 cut(s) 162, 414, 669
TasI AATT 2 cut(s) 625, 691
TatI WGTACW 1 cut(s) 393
TauI GCSGC 9 cut(s) 69, 104, 107, 143, 155, 198, 318, 832, 920
TfiI GAWTC 1 cut(s) 666
Tru1I TTAA 3 cut(s) 30, 426, 531
Tru9I TTAA 3 cut(s) 30, 426, 531
TscAI CASTG 1 cut(s) 715
TseFI GTSAC 1 cut(s) 755
TseI GCWGC 1 cut(s) 342
Tsp45I GTSAC 1 cut(s) 755
TspDTI ATGAA 1 cut(s) 862
TspGWI ACGGA 3 cut(s) 335, 753, 955
TspRI CASTG 1 cut(s) 715
VpaK11BI GGWCC 1 cut(s) 609
XcmI CCANNNNNNNNNTGG 1 cut(s) 544
XhoI CTCGAG 1 cut(s) 413
XmiI GTMKAC 2 cut(s) 204, 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.