AT2G21250

NADP-dependent D-sorbitol-6-phosphate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003235
Physical Location & Seq
Reverse (-)
9103136 .. 9105187
2052 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G21250.1

Sequence Viewer

Length: 930 bp
ATGGAGATAACACTGAACAGTGGCTTCAAGATGCCGATCGTCGGCTTAGGAGTATGGAGAATGGAGAAGGAAGGGATTCGAGACCTTATCCTCAATGCCATCAAGATCGGCTATCGCCATCTCGACTGTGCTGCTGATTATAGGAATGAGACCGAAGTTGGTGATGCTCTTACAGAAGCATTCAAGACTGGTCTGGTTAAGAGGGAGGATCTCTTCATTACAACCAAGCTATGGAATTCAGACCATGGCCATGTCATTGAGGCCTGCAAAGACAGTCTCAAGAAACTTCAACTTGATTACCTCGATCTCTTCCTCGTTCATTTTCCCGTAGCAACGAAACACACCGGAGTAGGGACAACCGATAGTGCTTTGGGCGATGACGGGGTATTGGACATAGACACGACCATCTCTCTGGAAACTACATGGCACGACATGGAAAAGCTTGTTTCTATGGGTTTAGTCCGCAGCATTGGAATCAGTAACTATGATGTCTTTCTAACGAGAGATTGCTTGGCTTACTCCAAGATCAAACCTGCTGTGAATCAGATCGAGACACATCCTTACTTCCAACGTGATTCTCTTGTCAAATTCTGCCAGAAACATGGTATCTGTGTCACTGCTCATACTCCTCTCGGAGGTGCCACAGCCAATGCCGAGTGGTTTGGCACCGTGTCATGCCTGGATGATCCAGTTCTCAAAGATGTGGCCGAGAAGTACAAAAAGACAGTGGCACAAGTTGTCCTGAGGTGGGGAATCCAGAGAAAAACGGTGGTGATACCAAAGACATCAAAGCCTGCGAGATTGGAAGAGAACTTTCAGGTTTTCGACTTCGAACTATCGAAAGAGGACATGGAAGTGATCAAAAGCATGGAGAGGAAATACCGCACGAACCAACCAGCCAAGTTTTGGAACATTGATCTCTACGCTTGA

Protein Analysis

309

Amino Acids

35.01

Weight (kDa)

6.16

Isoelectric Point (pI)

26.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 19 - 290 1.7e-52 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 541
AccB1I GGYRCC 2 cut(s) 638, 665
AccB7I CCANNNNNTGG 2 cut(s) 231, 906
AciI CCGC 2 cut(s) 463, 883
AclWI GGATC 2 cut(s) 216, 680
AcoI YGGCCR 2 cut(s) 247, 705
AcsI RAATTY 2 cut(s) 235, 587
AfaI GTAC 1 cut(s) 716
AfiI CCNNNNNNNGG 6 cut(s) 41, 231, 351, 635, 748, 906
AgsI TTSAA 3 cut(s) 28, 184, 290
AjnI CCWGG 1 cut(s) 678
AluBI AGCT 2 cut(s) 229, 442
AluI AGCT 2 cut(s) 229, 442
Alw26I GTCTC 4 cut(s) 75, 143, 281, 545
AlwI GGATC 2 cut(s) 216, 680
AoxI GGCC 3 cut(s) 247, 261, 705
ApeKI GCWGC 2 cut(s) 131, 465
ApoI RAATTY 2 cut(s) 235, 587
Asp700I GAANNNNTTC 1 cut(s) 75
AsuHPI GGTGA 2 cut(s) 173, 784
AsuII TTCGAA 1 cut(s) 831
AxyI CCTNAGG 1 cut(s) 743
BalI TGGCCA 1 cut(s) 249
BanI GGYRCC 2 cut(s) 638, 665
BbvI GCAGC 2 cut(s) 118, 477
BccI CCATC 3 cut(s) 107, 126, 413
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 1 cut(s) 680
BclI TGATCA 1 cut(s) 858
BcoDI GTCTC 4 cut(s) 75, 143, 281, 545
BfuAI ACCTGC 1 cut(s) 541
BisI GCNGC 2 cut(s) 132, 466
BlsI GCNGC 2 cut(s) 133, 467
Bme1390I CCNGG 1 cut(s) 680
BmiI GGNNCC 2 cut(s) 640, 667
BmrFI CCNGG 1 cut(s) 680
BmsI GCATC 2 cut(s) 21, 154
Bpu10I CCTNAGC 1 cut(s) 46
Bpu14I TTCGAA 1 cut(s) 831
BpuEI CTTGAG 1 cut(s) 263
BsaBI GATNNNNATC 1 cut(s) 35
BsaI GGTCTC 2 cut(s) 75, 143
BsaJI CCNNGG 1 cut(s) 244
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 6 cut(s) 41, 231, 351, 635, 748, 906
Bse1I ACTGG 2 cut(s) 193, 689
Bse21I CCTNAGG 1 cut(s) 743
Bse8I GATNNNNATC 1 cut(s) 35
BseBI CCWGG 1 cut(s) 680
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 2 cut(s) 556, 688
BseJI GATNNNNATC 1 cut(s) 35
BseLI CCNNNNNNNGG 6 cut(s) 41, 231, 351, 635, 748, 906
BseMII CTCAG 1 cut(s) 734
BseNI ACTGG 2 cut(s) 193, 689
BseRI GAGGAG 1 cut(s) 618
BseXI GCAGC 2 cut(s) 118, 477
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 3 cut(s) 249, 263, 707
BshNI GGYRCC 2 cut(s) 638, 665
BsiEI CGRYCG 1 cut(s) 39
BsiSI CCGG 1 cut(s) 345
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 6 cut(s) 41, 231, 351, 635, 748, 906
BsmAI GTCTC 4 cut(s) 75, 143, 281, 545
BsmFI GGGAC 1 cut(s) 367
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 3 cut(s) 249, 263, 707
Bso31I GGTCTC 2 cut(s) 75, 143
Bsp119I TTCGAA 1 cut(s) 831
Bsp143I GATC 9 cut(s) 36, 105, 208, 304, 525, 546, 685, 858, 916
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 2 cut(s) 463, 883
BspANI GGCC 3 cut(s) 249, 263, 707
BspCNI CTCAG 1 cut(s) 735
BspLI GGNNCC 2 cut(s) 640, 667
BspMI ACCTGC 1 cut(s) 541
BspPI GGATC 2 cut(s) 216, 680
BspT104I TTCGAA 1 cut(s) 831
BspT107I GGYRCC 2 cut(s) 638, 665
BspTNI GGTCTC 2 cut(s) 75, 143
BsrI ACTGG 2 cut(s) 193, 689
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 9 cut(s) 36, 105, 208, 304, 525, 546, 685, 858, 916
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 6 cut(s) 20, 128, 275, 670, 727, 769
Bst6I CTCTTC 3 cut(s) 218, 314, 801
BstBI TTCGAA 1 cut(s) 831
BstC8I GCNNGC 2 cut(s) 265, 795
BstDEI CTNAG 2 cut(s) 46, 743
BstDSI CCRYGG 1 cut(s) 244
BstENI CCTNNNNNAGG 1 cut(s) 633
BstF5I GGATG 2 cut(s) 556, 688
BstKTI GATC 9 cut(s) 39, 108, 211, 307, 528, 549, 688, 861, 919
BstMAI GTCTC 4 cut(s) 75, 143, 281, 545
BstMBI GATC 9 cut(s) 36, 105, 208, 304, 525, 546, 685, 858, 916
BstMCI CGRYCG 1 cut(s) 39
BstNI CCWGG 1 cut(s) 680
BstSCI CCNGG 1 cut(s) 678
BstV1I GCAGC 2 cut(s) 118, 477
BstX2I RGATCY 1 cut(s) 208
BstXI CCANNNNNNTGG 2 cut(s) 412, 602
BstYI RGATCY 1 cut(s) 208
Bsu36I CCTNAGG 1 cut(s) 743
BsuRI GGCC 3 cut(s) 249, 263, 707
BtgI CCRYGG 1 cut(s) 244
BtgZI GCGATG 1 cut(s) 390
BtsCI GGATG 2 cut(s) 556, 688
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 4 cut(s) 11, 25, 615, 732
BveI ACCTGC 1 cut(s) 541
Cac8I GCNNGC 2 cut(s) 265, 795
Csp6I GTAC 1 cut(s) 715
CviAII CATG 8 cut(s) 245, 251, 423, 433, 602, 675, 850, 868
CviQI GTAC 1 cut(s) 715
DdeI CTNAG 2 cut(s) 46, 743
DpnI GATC 9 cut(s) 38, 107, 210, 306, 527, 548, 687, 860, 918
DpnII GATC 9 cut(s) 36, 105, 208, 304, 525, 546, 685, 858, 916
EaeI YGGCCR 2 cut(s) 247, 705
Eam1104I CTCTTC 3 cut(s) 218, 314, 801
EarI CTCTTC 3 cut(s) 218, 314, 801
Eco130I CCWWGG 1 cut(s) 244
Eco147I AGGCCT 1 cut(s) 263
Eco31I GGTCTC 2 cut(s) 75, 143
Eco81I CCTNAGG 1 cut(s) 743
EcoNI CCTNNNNNAGG 1 cut(s) 633
EcoRI GAATTC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 678
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 8 cut(s) 248, 254, 426, 436, 605, 678, 853, 871
FaqI GGGAC 1 cut(s) 367
FatI CATG 8 cut(s) 244, 250, 422, 432, 601, 674, 849, 867
FbaI TGATCA 1 cut(s) 858
Fnu4HI GCNGC 2 cut(s) 132, 466
FokI GGATG 2 cut(s) 543, 695
Fsp4HI GCNGC 2 cut(s) 132, 466
GluI GCNGC 2 cut(s) 132, 466
HaeIII GGCC 3 cut(s) 249, 263, 707
HapII CCGG 1 cut(s) 345
Hin1II CATG 8 cut(s) 248, 254, 426, 436, 605, 678, 853, 871
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 5 cut(s) 76, 474, 541, 575, 753
HpaII CCGG 1 cut(s) 345
HphI GGTGA 2 cut(s) 173, 784
Hpy188I TCNGA 3 cut(s) 241, 546, 635
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 2 cut(s) 61, 65
HpyCH4III ACNGT 6 cut(s) 20, 128, 275, 670, 727, 769
HpyCH4IV ACGT 1 cut(s) 571
HpyCH4V TGCA 1 cut(s) 267
HpyF3I CTNAG 2 cut(s) 46, 743
HpySE526I ACGT 1 cut(s) 571
Hsp92II CATG 8 cut(s) 248, 254, 426, 436, 605, 678, 853, 871
Ksp22I TGATCA 1 cut(s) 858
Kzo9I GATC 9 cut(s) 36, 105, 208, 304, 525, 546, 685, 858, 916
Lsp1109I GCAGC 2 cut(s) 118, 477
LweI GCATC 2 cut(s) 21, 154
MaeII ACGT 1 cut(s) 571
MaeIII GTNAC 2 cut(s) 479, 613
MalI GATC 9 cut(s) 38, 107, 210, 306, 527, 548, 687, 860, 918
MboI GATC 9 cut(s) 36, 105, 208, 304, 525, 546, 685, 858, 916
MboII GAAGA 3 cut(s) 205, 301, 818
MflI RGATCY 1 cut(s) 208
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 2 cut(s) 235, 587
MluNI TGGCCA 1 cut(s) 249
MmeI TCCRAC 1 cut(s) 592
Mox20I TGGCCA 1 cut(s) 249
MroXI GAANNNNTTC 1 cut(s) 75
MscI TGGCCA 1 cut(s) 249
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 2 cut(s) 249, 854
Msp20I TGGCCA 1 cut(s) 249
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 680
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 1 cut(s) 680
NcoI CCATGG 1 cut(s) 244
NdeII GATC 9 cut(s) 36, 105, 208, 304, 525, 546, 685, 858, 916
NlaIII CATG 8 cut(s) 248, 254, 426, 436, 605, 678, 853, 871
NlaIV GGNNCC 2 cut(s) 640, 667
NmeAIII GCCGAG 2 cut(s) 679, 733
NmuCI GTSAC 1 cut(s) 613
NspV TTCGAA 1 cut(s) 831
PceI AGGCCT 1 cut(s) 263
PctI GAATGC 1 cut(s) 179
PdmI GAANNNNTTC 1 cut(s) 75
PfeI GAWTC 5 cut(s) 76, 474, 541, 575, 753
PflMI CCANNNNNTGG 2 cut(s) 231, 906
PkrI GCNGC 2 cut(s) 133, 467
Ple19I CGATCG 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 678
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 2 cut(s) 640, 667
PsuI RGATCY 1 cut(s) 208
PvuI CGATCG 1 cut(s) 39
RsaI GTAC 1 cut(s) 716
RsaNI GTAC 1 cut(s) 715
RseI CAYNNNNRTG 2 cut(s) 249, 854
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 2 cut(s) 132, 466
Sau3AI GATC 9 cut(s) 36, 105, 208, 304, 525, 546, 685, 858, 916
ScrFI CCNGG 1 cut(s) 680
SetI ASST 9 cut(s) 87, 231, 303, 444, 535, 574, 640, 749, 822
SfaNI GCATC 2 cut(s) 21, 154
SfuI TTCGAA 1 cut(s) 831
SmiMI CAYNNNNRTG 2 cut(s) 249, 854
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 2 cut(s) 235, 587
SseBI AGGCCT 1 cut(s) 263
SsiI CCGC 2 cut(s) 463, 883
StuI AGGCCT 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 678
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 6 cut(s) 20, 128, 275, 670, 727, 769
TaiI ACGT 1 cut(s) 574
TaqI TCGA 7 cut(s) 79, 123, 303, 549, 825, 831, 839
TaqII GACCGA 1 cut(s) 167
TasI AATT 2 cut(s) 235, 587
TatI WGTACW 1 cut(s) 714
TfiI GAWTC 5 cut(s) 76, 474, 541, 575, 753
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 4 cut(s) 18, 25, 622, 732
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 2 cut(s) 131, 465
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 2 cut(s) 205, 308
TspRI CASTG 4 cut(s) 18, 25, 622, 732
Van91I CCANNNNNTGG 2 cut(s) 231, 906
XagI CCTNNNNNAGG 1 cut(s) 633
XapI RAATTY 2 cut(s) 235, 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 903
XmnI GAANNNNTTC 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.