MD07G1054400.v1.1

NADP-dependent D-sorbitol-6-phosphate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003235
Physical Location & Seq
Forward (+)
5022528 .. 5026767
4240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1054400.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGCGATAACACTCAACAGTGGATTCAAGATGCCAGTAGTGGGGCTGGGTGTTTGGCGCATGGAAGGAAAAGAAATCAGAGACCTCGTTTTCAATGCCCTCAACCTAGGCTATCGCCATTTCGATTGCGCTGCTGATTACAAGAATGAACCAGAAGTTGGGGAGGCACTTGCAGAAGCACTTTCGACTGGGCTTGTTAAGCGAGAGGATCTCTTCATCACCACCAAGCTTTGGAATTCAGATCATGGACATGTGGTAGAGGCCTGTAAAGATAGTCTGAAGAAACTTCGGTTGGATTATCTAGATCTGTACCTTGTTCACTTTCCTGTTGCCACCAAGCATACCGGAGTTGGTACGACTGGCAGTGCTTTGGATGAGGATGGGGTGCTAGAAATAGACACCACTGTATCTTTGGAAACTACCTGGCATGCTATGGAAGAGCTGGTCTCTATGGGTTTAGTTCGTAGCATTGGAATTAGTAACTATGATATCTTTCTAACGAGAGATTGCTTAGTTTATTCCAAAGTGAAGCCGGCCGTGAATCAGATTGAGACTCACCCATACTTCCAGCGTGAATCTCTTGTCAAATTCTGTCAGAAGCATGGCATCTGTGTCACTGCCCACACTCCTCTTGGAGGTGCTGCCGCCAACATCGAATGGTTTGGTTCAGTTTCATGTCTGGATGATCCAGTTATCAAGAATCTGGCTGAGAAATACAAAAAGACTGTCGCCCAAGTTGTTCTAAGGTGGGGCATCCAACGCAACACGGTTGTCATCCCGAAGACATCAAAACTTGAGAGATTGAAAGAGAATATCCAAGTTTTCGACTTTGAGCTTACCAAAGAGGACATGGACCTGATTGGGAAGGTAGACAGGAAACACCGGACAAATCAACCTGCCAAGTTTTGGGGCGTTGATCTGTATGCATAG

Protein Analysis

310

Amino Acids

34.77

Weight (kDa)

6.22

Isoelectric Point (pI)

27.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 15 - 290 6e-54 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 904
AccB7I CCANNNNNTGG 3 cut(s) 158, 231, 906
AccI GTMKAC 1 cut(s) 870
AciI CCGC 1 cut(s) 645
AclWI GGATC 2 cut(s) 216, 680
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 2 cut(s) 235, 587
AcuI CTGAAG 1 cut(s) 299
AfaI GTAC 2 cut(s) 311, 355
AfiI CCNNNNNNNGG 5 cut(s) 41, 158, 231, 635, 906
AflIII ACRYGT 1 cut(s) 250
AgsI TTSAA 3 cut(s) 28, 94, 805
AjnI CCWGG 1 cut(s) 422
AjuI GAANNNNNNNTTGG 4 cut(s) 141, 173, 275, 307
AluBI AGCT 3 cut(s) 229, 442, 835
AluI AGCT 3 cut(s) 229, 442, 835
Alw26I GTCTC 3 cut(s) 75, 451, 545
AlwI GGATC 2 cut(s) 216, 680
AoxI GGCC 2 cut(s) 261, 534
ApeKI GCWGC 2 cut(s) 131, 641
ApoI RAATTY 2 cut(s) 235, 587
ArsI GACNNNNNNTTYG 2 cut(s) 711, 743
AspA2I CCTAGG 1 cut(s) 106
AspLEI GCGC 2 cut(s) 60, 131
AspS9I GGNCC 1 cut(s) 853
AsuHPI GGTGA 2 cut(s) 211, 548
AvaII GGWCC 1 cut(s) 853
AvrII CCTAGG 1 cut(s) 106
BaeI ACNNNNGTAYC 2 cut(s) 390, 423
BbsI GAAGAC 1 cut(s) 788
BbvI GCAGC 2 cut(s) 118, 628
BccI CCATC 1 cut(s) 374
BceAI ACGGC 1 cut(s) 521
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 1 cut(s) 424
BcoDI GTCTC 3 cut(s) 75, 451, 545
BfaI CTAG 3 cut(s) 107, 302, 389
BfuAI ACCTGC 1 cut(s) 904
BglII AGATCT 1 cut(s) 304
BisI GCNGC 3 cut(s) 132, 642, 645
BlnI CCTAGG 1 cut(s) 106
BlsI GCNGC 3 cut(s) 133, 643, 646
Bme1390I CCNGG 1 cut(s) 424
Bme18I GGWCC 1 cut(s) 853
BmgT120I GGNCC 1 cut(s) 853
BmrFI CCNGG 1 cut(s) 424
BmrI ACTGGG 1 cut(s) 198
BmsI GCATC 3 cut(s) 21, 615, 762
BmuI ACTGGG 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 788
BplI GAGNNNNNCTC 4 cut(s) 195, 227, 431, 463
BpuEI CTTGAG 1 cut(s) 815
BsaI GGTCTC 2 cut(s) 75, 451
BsaJI CCNNGG 1 cut(s) 106
BsaWI WCCGGW 2 cut(s) 344, 882
Bsc4I CCNNNNNNNGG 5 cut(s) 41, 158, 231, 635, 906
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 4 cut(s) 35, 193, 364, 689
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 5 cut(s) 379, 385, 688, 753, 774
BseLI CCNNNNNNNGG 5 cut(s) 41, 158, 231, 635, 906
BseMII CTCAG 1 cut(s) 699
BseNI ACTGG 4 cut(s) 35, 193, 364, 689
BseRI GAGGAG 1 cut(s) 618
BseX3I CGGCCG 1 cut(s) 534
BseXI GCAGC 2 cut(s) 118, 628
BseYI CCCAGC 1 cut(s) 46
Bsh1285I CGRYCG 1 cut(s) 537
BshFI GGCC 2 cut(s) 263, 536
BsiEI CGRYCG 1 cut(s) 537
BsiSI CCGG 3 cut(s) 345, 533, 883
BslI CCNNNNNNNGG 5 cut(s) 41, 158, 231, 635, 906
BsmAI GTCTC 3 cut(s) 75, 451, 545
BsnI GGCC 2 cut(s) 263, 536
Bso31I GGTCTC 2 cut(s) 75, 451
Bsp143I GATC 5 cut(s) 208, 241, 304, 685, 916
BspACI CCGC 1 cut(s) 645
BspANI GGCC 2 cut(s) 263, 536
BspCNI CTCAG 1 cut(s) 700
BspMI ACCTGC 1 cut(s) 904
BspPI GGATC 2 cut(s) 216, 680
BspQI GCTCTTC 1 cut(s) 432
BspTNI GGTCTC 2 cut(s) 75, 451
BsrFI RCCGGY 1 cut(s) 532
BsrI ACTGG 4 cut(s) 35, 193, 364, 689
BssAI RCCGGY 1 cut(s) 532
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 5 cut(s) 208, 241, 304, 685, 916
BssT1I CCWWGG 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 4 cut(s) 20, 406, 727, 769
Bst6I CTCTTC 2 cut(s) 218, 432
BstC8I GCNNGC 2 cut(s) 429, 534
BstDEI CTNAG 3 cut(s) 511, 708, 743
BstENI CCTNNNNNAGG 1 cut(s) 633
BstF5I GGATG 5 cut(s) 379, 385, 688, 753, 774
BstHHI GCGC 2 cut(s) 60, 131
BstKTI GATC 5 cut(s) 211, 244, 307, 688, 919
BstMAI GTCTC 3 cut(s) 75, 451, 545
BstMBI GATC 5 cut(s) 208, 241, 304, 685, 916
BstMCI CGRYCG 1 cut(s) 537
BstMWI GCNNNNNNNGC 2 cut(s) 199, 759
BstNI CCWGG 1 cut(s) 424
BstNSI RCATGY 2 cut(s) 254, 431
BstSCI CCNGG 1 cut(s) 422
BstV1I GCAGC 2 cut(s) 118, 628
BstV2I GAAGAC 1 cut(s) 788
BstX2I RGATCY 2 cut(s) 208, 304
BstYI RGATCY 2 cut(s) 208, 304
BstZI CGGCCG 1 cut(s) 534
BsuRI GGCC 2 cut(s) 263, 536
BtsCI GGATG 5 cut(s) 379, 385, 688, 753, 774
BtsI GCAGTG 2 cut(s) 370, 615
BtsIMutI CAGTG 4 cut(s) 25, 370, 402, 615
BveI ACCTGC 1 cut(s) 904
Cac8I GCNNGC 2 cut(s) 429, 534
CfoI GCGC 2 cut(s) 60, 131
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 1 cut(s) 853
Csp6I GTAC 2 cut(s) 310, 354
CviAII CATG 7 cut(s) 61, 245, 251, 428, 602, 675, 850
CviQI GTAC 2 cut(s) 310, 354
DdeI CTNAG 3 cut(s) 511, 708, 743
DpnI GATC 5 cut(s) 210, 243, 306, 687, 918
DpnII GATC 5 cut(s) 208, 241, 304, 685, 916
EaeI YGGCCR 1 cut(s) 534
EagI CGGCCG 1 cut(s) 534
Eam1104I CTCTTC 2 cut(s) 218, 432
EarI CTCTTC 2 cut(s) 218, 432
EclXI CGGCCG 1 cut(s) 534
Eco130I CCWWGG 1 cut(s) 106
Eco147I AGGCCT 1 cut(s) 263
Eco31I GGTCTC 2 cut(s) 75, 451
Eco32I GATATC 1 cut(s) 490
Eco47I GGWCC 1 cut(s) 853
Eco52I CGGCCG 1 cut(s) 534
Eco57I CTGAAG 1 cut(s) 299
EcoNI CCTNNNNNAGG 1 cut(s) 633
EcoRI GAATTC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 422
EcoRV GATATC 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 106
EcoT22I ATGCAT 1 cut(s) 928
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 7 cut(s) 64, 248, 254, 431, 605, 678, 853
FatI CATG 7 cut(s) 60, 244, 250, 427, 601, 674, 849
FblI GTMKAC 1 cut(s) 870
Fnu4HI GCNGC 3 cut(s) 132, 642, 645
FokI GGATG 5 cut(s) 386, 392, 695, 740, 761
Fsp4HI GCNGC 3 cut(s) 132, 642, 645
FspBI CTAG 3 cut(s) 107, 302, 389
GlaI GCGC 2 cut(s) 59, 130
GluI GCNGC 3 cut(s) 132, 642, 645
GsaI CCCAGC 1 cut(s) 50
HaeIII GGCC 2 cut(s) 263, 536
HapII CCGG 3 cut(s) 345, 533, 883
HhaI GCGC 2 cut(s) 60, 131
Hin1II CATG 7 cut(s) 64, 248, 254, 431, 605, 678, 853
Hin6I GCGC 2 cut(s) 58, 129
HinP1I GCGC 2 cut(s) 58, 129
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 5 cut(s) 24, 541, 553, 575, 700
HpaII CCGG 3 cut(s) 345, 533, 883
HphI GGTGA 2 cut(s) 211, 548
Hpy166II GTNNAC 2 cut(s) 319, 871
Hpy188I TCNGA 5 cut(s) 80, 241, 279, 546, 597
Hpy188III TCNNGA 5 cut(s) 28, 302, 680, 697, 778
Hpy8I GTNNAC 2 cut(s) 319, 871
HpyAV CCTTC 2 cut(s) 59, 859
HpyCH4III ACNGT 4 cut(s) 20, 406, 727, 769
HpyCH4V TGCA 2 cut(s) 173, 926
HpyF10VI GCNNNNNNNGC 2 cut(s) 199, 759
HpyF3I CTNAG 3 cut(s) 511, 708, 743
Hsp92II CATG 7 cut(s) 64, 248, 254, 431, 605, 678, 853
HspAI GCGC 2 cut(s) 58, 129
KroI GCCGGC 1 cut(s) 532
KroNI GCCGGC 1 cut(s) 534
Kzo9I GATC 5 cut(s) 208, 241, 304, 685, 916
LguI GCTCTTC 1 cut(s) 432
Lsp1109I GCAGC 2 cut(s) 118, 628
LweI GCATC 3 cut(s) 21, 615, 762
MaeI CTAG 3 cut(s) 107, 302, 389
MaeIII GTNAC 2 cut(s) 479, 613
MalI GATC 5 cut(s) 210, 243, 306, 687, 918
MboI GATC 5 cut(s) 208, 241, 304, 685, 916
MboII GAAGA 4 cut(s) 205, 292, 449, 793
MflI RGATCY 2 cut(s) 208, 304
MluCI AATT 3 cut(s) 235, 474, 587
MlyI GAGTC 1 cut(s) 547
MmeI TCCRAC 2 cut(s) 273, 781
MnlI CCTC 9 cut(s) 95, 110, 157, 199, 253, 370, 629, 639, 838
Mph1103I ATGCAT 1 cut(s) 928
MroNI GCCGGC 1 cut(s) 532
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 1 cut(s) 249
MspI CCGG 3 cut(s) 345, 533, 883
MspR9I CCNGG 1 cut(s) 424
MvaI CCWGG 1 cut(s) 424
MwoI GCNNNNNNNGC 2 cut(s) 199, 759
NaeI GCCGGC 1 cut(s) 534
NdeII GATC 5 cut(s) 208, 241, 304, 685, 916
NgoMIV GCCGGC 1 cut(s) 532
NlaIII CATG 7 cut(s) 64, 248, 254, 431, 605, 678, 853
NmuCI GTSAC 1 cut(s) 613
NsiI ATGCAT 1 cut(s) 928
NspI RCATGY 2 cut(s) 254, 431
PaeI GCATGC 1 cut(s) 431
PceI AGGCCT 1 cut(s) 263
PciI ACATGT 1 cut(s) 250
PciSI GCTCTTC 1 cut(s) 432
PdiI GCCGGC 1 cut(s) 534
PfeI GAWTC 4 cut(s) 24, 541, 575, 700
PflMI CCANNNNNTGG 3 cut(s) 158, 231, 906
PkrI GCNGC 3 cut(s) 133, 643, 646
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
PscI ACATGT 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 422
PspFI CCCAGC 1 cut(s) 46
PspGI CCWGG 1 cut(s) 422
PspPI GGNCC 1 cut(s) 853
PsuI RGATCY 2 cut(s) 208, 304
RsaI GTAC 2 cut(s) 311, 355
RsaNI GTAC 2 cut(s) 310, 354
RseI CAYNNNNRTG 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 432
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 3 cut(s) 132, 642, 645
Sau3AI GATC 5 cut(s) 208, 241, 304, 685, 916
Sau96I GGNCC 1 cut(s) 853
SchI GAGTC 1 cut(s) 547
ScrFI CCNGG 1 cut(s) 424
SfaNI GCATC 3 cut(s) 21, 615, 762
SinI GGWCC 1 cut(s) 853
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
SphI GCATGC 1 cut(s) 431
Sse9I AATT 3 cut(s) 235, 474, 587
SseBI AGGCCT 1 cut(s) 263
SsiI CCGC 1 cut(s) 645
SspMI CTAG 3 cut(s) 107, 302, 389
StuI AGGCCT 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 422
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 4 cut(s) 20, 406, 727, 769
TaqI TCGA 4 cut(s) 123, 185, 654, 825
TasI AATT 3 cut(s) 235, 474, 587
TauI GCSGC 1 cut(s) 647
TfiI GAWTC 4 cut(s) 24, 541, 575, 700
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 4 cut(s) 25, 370, 409, 622
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 2 cut(s) 131, 641
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 3 cut(s) 162, 205, 663
TspRI CASTG 4 cut(s) 25, 370, 409, 622
Van91I CCANNNNNTGG 3 cut(s) 158, 231, 906
VpaK11BI GGWCC 1 cut(s) 853
XagI CCTNNNNNAGG 1 cut(s) 633
XapI RAATTY 2 cut(s) 235, 587
XbaI TCTAGA 1 cut(s) 301
XceI RCATGY 2 cut(s) 254, 431
XcmI CCANNNNNNNNNTGG 3 cut(s) 409, 629, 847
XmaJI CCTAGG 1 cut(s) 106
XmiI GTMKAC 1 cut(s) 870
XspI CTAG 3 cut(s) 107, 302, 389
Zsp2I ATGCAT 1 cut(s) 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.