MD02G1264600.v1.1

NADP-dependent D-sorbitol-6-phosphate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003235
Physical Location & Seq
Forward (+)
31896846 .. 31903206
6361 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1264600.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGCGATAACACTCAACAGTGGGTTCAACATGCCAGTGTTGGGTTCGATTGCGCTGGTATCCAACTATCCATCGACAGTAGAAATAGATAGTAATATACAAGATATCCGGACTCGCTCCGGCCTTTGGCATCATACCAGTCGAAATTTGGAATCTGATTCCGTTCACAGATCATTCTTGTGCCTGCTAAAACATTTGAATCTACGTTCTCAGAATCATTTGTGCCCATATGGCGCTGATTACAAGAATGAACAAGAAGTTGGGGAGGCACTTGCAGAAGCATTTTCGACTGGGCTTGTTAAGCGAGAGGATCTCTTCATCACCACCAAGCTTTGGAATTCGGATCATGGACATGTTGTAGAGGCCTGTAAAGACAGTCTGAAGAAGCTTCGGTTGGATTATCTGGATCTGTACCTTGTTCACTTTCCTGTTGCCACCAAGCATACCGGAGTTGGTACGACTGGCAGTGCTTTGGATGAGGATGGGGTGCTAGAAATAGACACAACCATATCTTTGGAAACTACCTGGCATGCTATGGAAGAGCTAGTCTCTATGGGTTTAGTTCGTAGCATTGGAATTAGTAACTATGATATCTTTCTCACGAGAGATTGCTTAGCTTATTCCAAAGTGAAGCCCGCCGTGAATCAGATTGAGACTCACCCATACTTCCAACGTGAATCTCTTGTCAAATTCTGTCAGAAGCATGGCATCTGTGTCACTGCCCACACTCCTCTTGGAGGTGCTGTGGCCAAAAGCGAGTGGTTTGGTTCAGTTTCATGTCTAGACGATCCAGTTCTTAAGAATCTGGCTGAGAAATACAAAAAGACTGCTGCCCAAGTTGTTCTAAGGTGGGGCATCGAACGGAACACAGTTGTCATCCCGAAGACGTCAAAACTTGAGAGATTGAAAGAGAATTTCCAAGTTTTGACTTTGAGCTCACCAAAGAGGACATGGACCTGA

Protein Analysis

320

Amino Acids

35.76

Weight (kDa)

6.89

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 81 - 311 1.8e-43 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 890
AccB7I CCANNNNNTGG 1 cut(s) 333
AccIII TCCGGA 1 cut(s) 108
AciI CCGC 1 cut(s) 636
AclWI GGATC 4 cut(s) 318, 351, 414, 782
AcoI YGGCCR 1 cut(s) 747
AcsI RAATTY 4 cut(s) 145, 337, 689, 913
AcuI CTGAAG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 887
AfaI GTAC 2 cut(s) 413, 457
AfiI CCNNNNNNNGG 4 cut(s) 41, 126, 333, 737
AflII CTTAAG 1 cut(s) 797
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 3 cut(s) 28, 199, 907
AjnI CCWGG 1 cut(s) 524
AjuI GAANNNNNNNTTGG 4 cut(s) 243, 275, 377, 409
AluBI AGCT 5 cut(s) 331, 388, 544, 617, 936
AluI AGCT 5 cut(s) 331, 388, 544, 617, 936
Alw21I GWGCWC 1 cut(s) 938
Alw26I GTCTC 2 cut(s) 553, 647
AlwI GGATC 4 cut(s) 318, 351, 414, 782
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 3 cut(s) 121, 363, 747
ApeKI GCWGC 1 cut(s) 830
ApoI RAATTY 4 cut(s) 145, 337, 689, 913
AspLEI GCGC 2 cut(s) 55, 236
AspS9I GGNCC 1 cut(s) 954
AsuHPI GGTGA 3 cut(s) 313, 650, 930
AvaII GGWCC 1 cut(s) 954
BaeGI GKGCMC 1 cut(s) 227
BalI TGGCCA 1 cut(s) 749
BanII GRGCYC 1 cut(s) 938
BauI CACGAG 1 cut(s) 601
BbsI GAAGAC 1 cut(s) 890
Bbv12I GWGCWC 1 cut(s) 938
BbvI GCAGC 1 cut(s) 817
BccI CCATC 2 cut(s) 79, 476
BceAI ACGGC 1 cut(s) 623
BciT130I CCWGG 1 cut(s) 526
BciVI GTATCC 1 cut(s) 70
BcoDI GTCTC 2 cut(s) 553, 647
BfaI CTAG 3 cut(s) 491, 545, 782
BfoI RGCGCY 1 cut(s) 237
BfrI CTTAAG 1 cut(s) 797
BfuI GTATCC 1 cut(s) 70
BglI GCCNNNNNGGC 1 cut(s) 231
BisI GCNGC 1 cut(s) 831
BlpI GCTNAGC 1 cut(s) 613
BlsI GCNGC 1 cut(s) 832
Bme1390I CCNGG 1 cut(s) 526
Bme18I GGWCC 1 cut(s) 954
BmgT120I GGNCC 1 cut(s) 954
BmrFI CCNGG 1 cut(s) 526
BmrI ACTGGG 1 cut(s) 300
BmsI GCATC 3 cut(s) 139, 717, 864
BmuI ACTGGG 1 cut(s) 300
BpiI GAAGAC 1 cut(s) 890
BplI GAGNNNNNCTC 4 cut(s) 297, 329, 533, 565
Bpu1102I GCTNAGC 1 cut(s) 613
BpuEI CTTGAG 1 cut(s) 917
BsaHI GRCGYC 1 cut(s) 887
BsaWI WCCGGW 2 cut(s) 108, 446
BsaXI ACNNNNNCTCC 2 cut(s) 729, 759
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 126, 333, 737
Bse1I ACTGG 5 cut(s) 35, 138, 295, 466, 791
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 1 cut(s) 526
BseGI GGATG 3 cut(s) 481, 487, 876
BseLI CCNNNNNNNGG 4 cut(s) 41, 126, 333, 737
BseMII CTCAG 2 cut(s) 224, 801
BseNI ACTGG 5 cut(s) 35, 138, 295, 466, 791
BseRI GAGGAG 1 cut(s) 720
BseSI GKGCMC 1 cut(s) 227
BseXI GCAGC 1 cut(s) 817
BshFI GGCC 3 cut(s) 123, 365, 749
BsiHKAI GWGCWC 1 cut(s) 938
BsiSI CCGG 3 cut(s) 109, 120, 447
BslI CCNNNNNNNGG 4 cut(s) 41, 126, 333, 737
BsmAI GTCTC 2 cut(s) 553, 647
BsnI GGCC 3 cut(s) 123, 365, 749
Bsp1286I GDGCHC 2 cut(s) 227, 938
Bsp13I TCCGGA 1 cut(s) 108
Bsp143I GATC 5 cut(s) 170, 310, 343, 406, 787
Bsp1720I GCTNAGC 1 cut(s) 613
BspACI CCGC 1 cut(s) 636
BspANI GGCC 3 cut(s) 123, 365, 749
BspCNI CTCAG 2 cut(s) 223, 802
BspEI TCCGGA 1 cut(s) 108
BspPI GGATC 4 cut(s) 318, 351, 414, 782
BspQI GCTCTTC 1 cut(s) 534
BspTI CTTAAG 1 cut(s) 797
BsrI ACTGG 5 cut(s) 35, 138, 295, 466, 791
BssMI GATC 5 cut(s) 170, 310, 343, 406, 787
BssNI GRCGYC 1 cut(s) 887
BssSI CACGAG 1 cut(s) 601
Bst2BI CACGAG 1 cut(s) 601
Bst2UI CCWGG 1 cut(s) 526
Bst4CI ACNGT 4 cut(s) 20, 79, 377, 871
Bst6I CTCTTC 2 cut(s) 320, 534
BstACI GRCGYC 1 cut(s) 887
BstAFI CTTAAG 1 cut(s) 797
BstC8I GCNNGC 3 cut(s) 185, 531, 636
BstDEI CTNAG 4 cut(s) 210, 613, 810, 845
BstENI CCTNNNNNAGG 1 cut(s) 735
BstF5I GGATG 3 cut(s) 481, 487, 876
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 2 cut(s) 55, 236
BstKTI GATC 5 cut(s) 173, 313, 346, 409, 790
BstMAI GTCTC 2 cut(s) 553, 647
BstMBI GATC 5 cut(s) 170, 310, 343, 406, 787
BstMWI GCNNNNNNNGC 2 cut(s) 231, 301
BstNI CCWGG 1 cut(s) 526
BstNSI RCATGY 3 cut(s) 34, 356, 533
BstSCI CCNGG 1 cut(s) 524
BstSLI GKGCMC 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 817
BstV2I GAAGAC 1 cut(s) 890
BstX2I RGATCY 2 cut(s) 310, 406
BstXI CCANNNNNNTGG 1 cut(s) 514
BstYI RGATCY 2 cut(s) 310, 406
BsuI GTATCC 1 cut(s) 70
BsuRI GGCC 3 cut(s) 123, 365, 749
BtsCI GGATG 3 cut(s) 481, 487, 876
BtsI GCAGTG 2 cut(s) 472, 717
BtsIMutI CAGTG 4 cut(s) 25, 42, 472, 717
Cac8I GCNNGC 3 cut(s) 185, 531, 636
CfoI GCGC 2 cut(s) 55, 236
Cfr13I GGNCC 1 cut(s) 954
Csp6I GTAC 2 cut(s) 412, 456
CviAII CATG 7 cut(s) 31, 347, 353, 530, 704, 777, 951
CviQI GTAC 2 cut(s) 412, 456
DdeI CTNAG 4 cut(s) 210, 613, 810, 845
DpnI GATC 5 cut(s) 172, 312, 345, 408, 789
DpnII GATC 5 cut(s) 170, 310, 343, 406, 787
EaeI YGGCCR 1 cut(s) 747
Eam1104I CTCTTC 2 cut(s) 320, 534
EarI CTCTTC 2 cut(s) 320, 534
Ecl136II GAGCTC 1 cut(s) 936
Eco147I AGGCCT 1 cut(s) 365
Eco24I GRGCYC 1 cut(s) 938
Eco32I GATATC 2 cut(s) 106, 592
Eco47I GGWCC 1 cut(s) 954
Eco53kI GAGCTC 1 cut(s) 936
Eco57I CTGAAG 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 936
EcoNI CCTNNNNNAGG 1 cut(s) 735
EcoRI GAATTC 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 524
EcoRV GATATC 2 cut(s) 106, 592
EcoT38I GRGCYC 1 cut(s) 938
FaeI CATG 7 cut(s) 34, 350, 356, 533, 707, 780, 954
FatI CATG 7 cut(s) 30, 346, 352, 529, 703, 776, 950
FauI CCCGC 1 cut(s) 643
FauNDI CATATG 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 831
FokI GGATG 3 cut(s) 488, 494, 863
FriOI GRGCYC 1 cut(s) 938
Fsp4HI GCNGC 1 cut(s) 831
FspBI CTAG 3 cut(s) 491, 545, 782
GlaI GCGC 2 cut(s) 54, 235
GluI GCNGC 1 cut(s) 831
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 3 cut(s) 123, 365, 749
HapII CCGG 3 cut(s) 109, 120, 447
HhaI GCGC 2 cut(s) 55, 236
Hin1I GRCGYC 1 cut(s) 887
Hin1II CATG 7 cut(s) 34, 350, 356, 533, 707, 780, 954
Hin6I GCGC 2 cut(s) 53, 234
HinP1I GCGC 2 cut(s) 53, 234
HindIII AAGCTT 2 cut(s) 329, 386
HinfI GANTC 9 cut(s) 112, 152, 158, 199, 214, 643, 655, 677, 802
HpaII CCGG 3 cut(s) 109, 120, 447
HphI GGTGA 3 cut(s) 313, 650, 930
Hpy166II GTNNAC 2 cut(s) 166, 421
Hpy188I TCNGA 6 cut(s) 157, 213, 343, 381, 648, 699
Hpy188III TCNNGA 5 cut(s) 109, 404, 601, 782, 880
Hpy8I GTNNAC 2 cut(s) 166, 421
HpyCH4III ACNGT 4 cut(s) 20, 79, 377, 871
HpyCH4IV ACGT 3 cut(s) 205, 673, 887
HpyCH4V TGCA 1 cut(s) 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 301
HpyF3I CTNAG 4 cut(s) 210, 613, 810, 845
HpySE526I ACGT 3 cut(s) 205, 673, 887
Hsp92I GRCGYC 1 cut(s) 887
Hsp92II CATG 7 cut(s) 34, 350, 356, 533, 707, 780, 954
HspAI GCGC 2 cut(s) 53, 234
Kpn2I TCCGGA 1 cut(s) 108
Kzo9I GATC 5 cut(s) 170, 310, 343, 406, 787
LguI GCTCTTC 1 cut(s) 534
LmnI GCTCC 1 cut(s) 122
Lsp1109I GCAGC 1 cut(s) 817
LweI GCATC 3 cut(s) 139, 717, 864
MaeI CTAG 3 cut(s) 491, 545, 782
MaeII ACGT 3 cut(s) 205, 673, 887
MaeIII GTNAC 2 cut(s) 581, 715
MalI GATC 5 cut(s) 172, 312, 345, 408, 789
MboI GATC 5 cut(s) 170, 310, 343, 406, 787
MboII GAAGA 4 cut(s) 307, 394, 551, 895
MflI RGATCY 2 cut(s) 310, 406
MhlI GDGCHC 2 cut(s) 227, 938
MlsI TGGCCA 1 cut(s) 749
MluCI AATT 5 cut(s) 145, 337, 576, 689, 913
MluNI TGGCCA 1 cut(s) 749
MlyI GAGTC 2 cut(s) 106, 649
MmeI TCCRAC 3 cut(s) 87, 375, 694
MnlI CCTC 7 cut(s) 259, 301, 355, 472, 731, 741, 939
Mox20I TGGCCA 1 cut(s) 749
MroI TCCGGA 1 cut(s) 108
MscI TGGCCA 1 cut(s) 749
MseI TTAA 2 cut(s) 300, 798
MslI CAYNNNNRTG 3 cut(s) 35, 178, 351
Msp20I TGGCCA 1 cut(s) 749
MspCI CTTAAG 1 cut(s) 797
MspI CCGG 3 cut(s) 109, 120, 447
MspR9I CCNGG 1 cut(s) 526
MvaI CCWGG 1 cut(s) 526
MwoI GCNNNNNNNGC 2 cut(s) 231, 301
NdeI CATATG 1 cut(s) 229
NdeII GATC 5 cut(s) 170, 310, 343, 406, 787
NlaIII CATG 7 cut(s) 34, 350, 356, 533, 707, 780, 954
NmuCI GTSAC 1 cut(s) 715
NspI RCATGY 3 cut(s) 34, 356, 533
PaeI GCATGC 1 cut(s) 533
PceI AGGCCT 1 cut(s) 365
PciI ACATGT 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 534
PfeI GAWTC 7 cut(s) 152, 158, 199, 214, 643, 677, 802
PflMI CCANNNNNTGG 1 cut(s) 333
PkrI GCNGC 1 cut(s) 832
PleI GAGTC 2 cut(s) 106, 649
PpsI GAGTC 2 cut(s) 106, 649
PscI ACATGT 1 cut(s) 352
Psp124BI GAGCTC 1 cut(s) 938
Psp6I CCWGG 1 cut(s) 524
PspGI CCWGG 1 cut(s) 524
PspPI GGNCC 1 cut(s) 954
PsuI RGATCY 2 cut(s) 310, 406
RsaI GTAC 2 cut(s) 413, 457
RsaNI GTAC 2 cut(s) 412, 456
RseI CAYNNNNRTG 3 cut(s) 35, 178, 351
SacI GAGCTC 1 cut(s) 938
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 2 cut(s) 300, 798
SatI GCNGC 1 cut(s) 831
Sau3AI GATC 5 cut(s) 170, 310, 343, 406, 787
Sau96I GGNCC 1 cut(s) 954
SchI GAGTC 2 cut(s) 106, 649
ScrFI CCNGG 1 cut(s) 526
SduI GDGCHC 2 cut(s) 227, 938
SfaNI GCATC 3 cut(s) 139, 717, 864
SinI GGWCC 1 cut(s) 954
SmiMI CAYNNNNRTG 3 cut(s) 35, 178, 351
SmlI CTYRAG 2 cut(s) 797, 896
SmoI CTYRAG 2 cut(s) 797, 896
SphI GCATGC 1 cut(s) 533
Sse9I AATT 5 cut(s) 145, 337, 576, 689, 913
SseBI AGGCCT 1 cut(s) 365
SsiI CCGC 1 cut(s) 636
SspMI CTAG 3 cut(s) 491, 545, 782
SstI GAGCTC 1 cut(s) 938
StuI AGGCCT 1 cut(s) 365
StyD4I CCNGG 1 cut(s) 524
TaaI ACNGT 4 cut(s) 20, 79, 377, 871
TaiI ACGT 3 cut(s) 208, 676, 890
TaqI TCGA 5 cut(s) 47, 74, 142, 287, 858
TasI AATT 5 cut(s) 145, 337, 576, 689, 913
TfiI GAWTC 7 cut(s) 152, 158, 199, 214, 643, 677, 802
Tru1I TTAA 2 cut(s) 300, 798
Tru9I TTAA 2 cut(s) 300, 798
TscAI CASTG 4 cut(s) 25, 42, 472, 724
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 1 cut(s) 830
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 3 cut(s) 264, 307, 765
TspGWI ACGGA 2 cut(s) 151, 877
TspRI CASTG 4 cut(s) 25, 42, 472, 724
Van91I CCANNNNNTGG 1 cut(s) 333
Vha464I CTTAAG 1 cut(s) 797
VpaK11BI GGWCC 1 cut(s) 954
XagI CCTNNNNNAGG 1 cut(s) 735
XapI RAATTY 4 cut(s) 145, 337, 689, 913
XbaI TCTAGA 1 cut(s) 781
XceI RCATGY 3 cut(s) 34, 356, 533
XcmI CCANNNNNNNNNTGG 3 cut(s) 145, 731, 948
XspI CTAG 3 cut(s) 491, 545, 782
ZraI GACGTC 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.