Rh6DG150500

NADP-dependent D-sorbitol-6-phosphate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003235
Physical Location & Seq
Forward (+)
21771343 .. 21775214
3872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG150500.1

Sequence Viewer

Length: 837 bp
ATGTATGTGATGTACATTGACAAGATAATTCTGCTTTCAGCTGATTACAAGAATGAATCAGAAGTTGGAGAGGCACTAGCAGAAGCATTTTCCACTGGGCTTGTTAAGCGAGAGGATCTCTTCATCACCACCAAGCTTTGGAATTCAGATCACGGACATGTTGTGGAGGCCTGTAAAGACAGTCTAAAGAAGCTTCGGTTGGATTATCTAGATCTGTACCTTGTTCACTTTCCTGTTGCCACGAAGCACACCGGAGTTGGTGCAACTGACAGTGCTTTGGATGAGGATGGGGTGCTAGAGATAGACACAACCATTTCTCTAGAAACTACTTGGCGTGCTATGGAAGATCTGGTTTCTCTGGGTTTAGTTCGTAGTATTGGGATCAGCAACTATGACATCTTTCTCACTAGAGATTGCTTGGCCTATTCCAAAGTGAAGCCTGCTGTGAATCAGATTGAAACTCACCCATACTTCCAGCGTGATTCTCTTGTCAAATTCTGTCTGAAACATGGCATCTCCGTCACAGCCCACACTCCACTTGGAGGTGCTGCAGCCAACACTGAATGGTTTGGTTCAGTTTCAGCTCTGGATGATCCAGTTCTCAAGAATCTTGCTGAGAAATACAAAAAGACAGTGGCCCAGGTTGTTCTTCGATGGGGCATTCAACGCAACACGGTTGTAATCCCGAAGACGTCAAAACTCGAGAGATTGAAAGAGAACTTTCAAGTCTTTGACTTTGAGATTTCCAAAGAGGACATGGACCTCATTAAAAAGGTGGACAGGAAACTCCGGACCAATCAACCTGCCAAGTTTTGGGGCGTTGATCTATTTGCTTAG

Protein Analysis

278

Amino Acids

31.39

Weight (kDa)

6.01

Isoelectric Point (pI)

22.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 16 - 260 1.4e-47 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 695
Acc36I ACCTGC 1 cut(s) 811
AccB7I CCANNNNNTGG 2 cut(s) 138, 813
AccIII TCCGGA 1 cut(s) 789
AclWI GGATC 3 cut(s) 123, 389, 587
AcsI RAATTY 2 cut(s) 142, 494
AcyI GRCGYC 1 cut(s) 692
AfaI GTAC 2 cut(s) 14, 218
AfiI CCNNNNNNNGG 3 cut(s) 138, 542, 813
AflIII ACRYGT 1 cut(s) 157
AgsI TTSAA 4 cut(s) 458, 665, 712, 725
AjnI CCWGG 1 cut(s) 639
AjuI GAANNNNNNNTTGG 4 cut(s) 48, 80, 182, 214
AluBI AGCT 4 cut(s) 41, 136, 193, 584
AluI AGCT 4 cut(s) 41, 136, 193, 584
AlwI GGATC 3 cut(s) 123, 389, 587
Ama87I CYCGRG 1 cut(s) 701
Aor13HI TCCGGA 1 cut(s) 789
AoxI GGCC 3 cut(s) 168, 420, 636
ApeKI GCWGC 2 cut(s) 548, 551
ApoI RAATTY 2 cut(s) 142, 494
AspS9I GGNCC 3 cut(s) 637, 760, 792
AsuHPI GGTGA 2 cut(s) 118, 455
AvaI CYCGRG 1 cut(s) 701
AvaII GGWCC 2 cut(s) 760, 792
BbsI GAAGAC 1 cut(s) 695
BbvI GCAGC 2 cut(s) 535, 563
BccI CCATC 2 cut(s) 281, 648
BciT130I CCWGG 1 cut(s) 641
BfaI CTAG 5 cut(s) 77, 209, 296, 320, 408
BfmI CTRYAG 1 cut(s) 549
BfuAI ACCTGC 1 cut(s) 811
BglII AGATCT 2 cut(s) 211, 346
BisI GCNGC 2 cut(s) 549, 552
BlsI GCNGC 2 cut(s) 550, 553
Bme1390I CCNGG 1 cut(s) 641
Bme18I GGWCC 2 cut(s) 760, 792
BmeT110I CYCGRG 1 cut(s) 701
BmgT120I GGNCC 3 cut(s) 637, 760, 792
BmrFI CCNGG 1 cut(s) 641
BmrI ACTGGG 1 cut(s) 105
BmsI GCATC 1 cut(s) 522
BmuI ACTGGG 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 695
BplI GAGNNNNNCTC 2 cut(s) 102, 134
BpuEI CTTGAG 1 cut(s) 587
BsaHI GRCGYC 1 cut(s) 692
BsaJI CCNNGG 1 cut(s) 639
BsaWI WCCGGW 2 cut(s) 251, 789
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 542, 813
Bse1I ACTGG 2 cut(s) 100, 596
BseAI TCCGGA 1 cut(s) 789
BseBI CCWGG 1 cut(s) 641
BseDI CCNNGG 1 cut(s) 639
BseGI GGATG 3 cut(s) 286, 292, 595
BseLI CCNNNNNNNGG 3 cut(s) 138, 542, 813
BseMII CTCAG 1 cut(s) 606
BseNI ACTGG 2 cut(s) 100, 596
BseXI GCAGC 2 cut(s) 535, 563
BshFI GGCC 3 cut(s) 170, 422, 638
BsiHKCI CYCGRG 1 cut(s) 701
BsiSI CCGG 2 cut(s) 252, 790
BslI CCNNNNNNNGG 3 cut(s) 138, 542, 813
BsmI GAATGC 1 cut(s) 660
BsnI GGCC 3 cut(s) 170, 422, 638
BsoBI CYCGRG 1 cut(s) 701
Bsp13I TCCGGA 1 cut(s) 789
Bsp1407I TGTACA 1 cut(s) 12
Bsp143I GATC 7 cut(s) 115, 148, 211, 346, 381, 592, 823
BspANI GGCC 3 cut(s) 170, 422, 638
BspCNI CTCAG 1 cut(s) 607
BspEI TCCGGA 1 cut(s) 789
BspMAI CTGCAG 1 cut(s) 553
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 3 cut(s) 123, 389, 587
BsrGI TGTACA 1 cut(s) 12
BsrI ACTGG 2 cut(s) 100, 596
BssECI CCNNGG 1 cut(s) 639
BssMI GATC 7 cut(s) 115, 148, 211, 346, 381, 592, 823
BssNI GRCGYC 1 cut(s) 692
Bst2UI CCWGG 1 cut(s) 641
Bst4CI ACNGT 4 cut(s) 182, 272, 634, 676
Bst6I CTCTTC 1 cut(s) 125
BstACI GRCGYC 1 cut(s) 692
BstAUI TGTACA 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 336, 441
BstDEI CTNAG 2 cut(s) 615, 834
BstF5I GGATG 3 cut(s) 286, 292, 595
BstKTI GATC 7 cut(s) 118, 151, 214, 349, 384, 595, 826
BstMBI GATC 7 cut(s) 115, 148, 211, 346, 381, 592, 823
BstMWI GCNNNNNNNGC 2 cut(s) 106, 666
BstNI CCWGG 1 cut(s) 641
BstNSI RCATGY 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 639
BstSFI CTRYAG 1 cut(s) 549
BstV1I GCAGC 2 cut(s) 535, 563
BstV2I GAAGAC 1 cut(s) 695
BstX2I RGATCY 3 cut(s) 115, 211, 346
BstYI RGATCY 3 cut(s) 115, 211, 346
BsuRI GGCC 3 cut(s) 170, 422, 638
BtsCI GGATG 3 cut(s) 286, 292, 595
BtsIMutI CAGTG 4 cut(s) 93, 277, 558, 639
BveI ACCTGC 1 cut(s) 811
Cac8I GCNNGC 2 cut(s) 336, 441
Cfr13I GGNCC 3 cut(s) 637, 760, 792
Csp6I GTAC 2 cut(s) 13, 217
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviAII CATG 3 cut(s) 158, 509, 757
CviQI GTAC 2 cut(s) 13, 217
DdeI CTNAG 2 cut(s) 615, 834
DpnI GATC 7 cut(s) 117, 150, 213, 348, 383, 594, 825
DpnII GATC 7 cut(s) 115, 148, 211, 346, 381, 592, 823
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Eco147I AGGCCT 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 760, 792
Eco88I CYCGRG 1 cut(s) 701
EcoRI GAATTC 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 639
FaeI CATG 3 cut(s) 161, 512, 760
FaiI YATR 7 cut(s) 6, 159, 341, 393, 469, 510, 758
FatI CATG 3 cut(s) 157, 508, 756
Fnu4HI GCNGC 2 cut(s) 549, 552
FokI GGATG 3 cut(s) 293, 299, 602
Fsp4HI GCNGC 2 cut(s) 549, 552
FspBI CTAG 5 cut(s) 77, 209, 296, 320, 408
GluI GCNGC 2 cut(s) 549, 552
HaeIII GGCC 3 cut(s) 170, 422, 638
HapII CCGG 2 cut(s) 252, 790
Hin1I GRCGYC 1 cut(s) 692
Hin1II CATG 3 cut(s) 161, 512, 760
HindIII AAGCTT 2 cut(s) 134, 191
HinfI GANTC 4 cut(s) 56, 448, 482, 607
HpaII CCGG 2 cut(s) 252, 790
HphI GGTGA 2 cut(s) 118, 455
Hpy166II GTNNAC 2 cut(s) 226, 778
Hpy188I TCNGA 4 cut(s) 61, 148, 453, 504
Hpy188III TCNNGA 7 cut(s) 209, 320, 587, 604, 685, 703, 790
Hpy8I GTNNAC 2 cut(s) 226, 778
HpyCH4III ACNGT 4 cut(s) 182, 272, 634, 676
HpyCH4IV ACGT 1 cut(s) 692
HpyCH4V TGCA 2 cut(s) 263, 551
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 666
HpyF3I CTNAG 2 cut(s) 615, 834
HpySE526I ACGT 1 cut(s) 692
Hsp92I GRCGYC 1 cut(s) 692
Hsp92II CATG 3 cut(s) 161, 512, 760
Kpn2I TCCGGA 1 cut(s) 789
Kzo9I GATC 7 cut(s) 115, 148, 211, 346, 381, 592, 823
Lsp1109I GCAGC 2 cut(s) 535, 563
LweI GCATC 1 cut(s) 522
MaeI CTAG 5 cut(s) 77, 209, 296, 320, 408
MaeII ACGT 1 cut(s) 692
MaeIII GTNAC 1 cut(s) 520
MalI GATC 7 cut(s) 117, 150, 213, 348, 383, 594, 825
MboI GATC 7 cut(s) 115, 148, 211, 346, 381, 592, 823
MboII GAAGA 4 cut(s) 112, 356, 641, 700
MflI RGATCY 3 cut(s) 115, 211, 346
MluCI AATT 3 cut(s) 27, 142, 494
MmeI TCCRAC 2 cut(s) 46, 180
MnlI CCTC 7 cut(s) 64, 106, 160, 277, 536, 745, 773
MroI TCCGGA 1 cut(s) 789
MseI TTAA 2 cut(s) 105, 768
MslI CAYNNNNRTG 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 41
MspI CCGG 2 cut(s) 252, 790
MspR9I CCNGG 1 cut(s) 641
Mva1269I GAATGC 1 cut(s) 660
MvaI CCWGG 1 cut(s) 641
MwoI GCNNNNNNNGC 2 cut(s) 106, 666
NdeII GATC 7 cut(s) 115, 148, 211, 346, 381, 592, 823
NlaIII CATG 3 cut(s) 161, 512, 760
NmuCI GTSAC 1 cut(s) 520
NspI RCATGY 1 cut(s) 161
PaeR7I CTCGAG 1 cut(s) 701
PceI AGGCCT 1 cut(s) 170
PciI ACATGT 1 cut(s) 157
PctI GAATGC 1 cut(s) 660
PfeI GAWTC 4 cut(s) 56, 448, 482, 607
PflMI CCANNNNNTGG 2 cut(s) 138, 813
PkrI GCNGC 2 cut(s) 550, 553
PscI ACATGT 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 639
PspGI CCWGG 1 cut(s) 639
PspPI GGNCC 3 cut(s) 637, 760, 792
PstI CTGCAG 1 cut(s) 553
PsuI RGATCY 3 cut(s) 115, 211, 346
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 2 cut(s) 14, 218
RsaNI GTAC 2 cut(s) 13, 217
RseI CAYNNNNRTG 1 cut(s) 156
SaqAI TTAA 2 cut(s) 105, 768
SatI GCNGC 2 cut(s) 549, 552
Sau3AI GATC 7 cut(s) 115, 148, 211, 346, 381, 592, 823
Sau96I GGNCC 3 cut(s) 637, 760, 792
ScrFI CCNGG 1 cut(s) 641
SfaNI GCATC 1 cut(s) 522
SfcI CTRYAG 1 cut(s) 549
Sfr274I CTCGAG 1 cut(s) 701
SinI GGWCC 2 cut(s) 760, 792
SlaI CTCGAG 1 cut(s) 701
SmiMI CAYNNNNRTG 1 cut(s) 156
SmlI CTYRAG 2 cut(s) 602, 701
SmoI CTYRAG 2 cut(s) 602, 701
Sse9I AATT 3 cut(s) 27, 142, 494
SseBI AGGCCT 1 cut(s) 170
SspMI CTAG 5 cut(s) 77, 209, 296, 320, 408
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 639
TaaI ACNGT 4 cut(s) 182, 272, 634, 676
TaiI ACGT 1 cut(s) 695
TaqI TCGA 2 cut(s) 652, 702
TasI AATT 3 cut(s) 27, 142, 494
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 4 cut(s) 56, 448, 482, 607
Tru1I TTAA 2 cut(s) 105, 768
Tru9I TTAA 2 cut(s) 105, 768
TscAI CASTG 4 cut(s) 100, 277, 565, 639
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 2 cut(s) 548, 551
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 2 cut(s) 69, 112
TspGWI ACGGA 2 cut(s) 168, 508
TspRI CASTG 4 cut(s) 100, 277, 565, 639
Van91I CCANNNNNTGG 2 cut(s) 138, 813
VpaK11BI GGWCC 2 cut(s) 760, 792
XapI RAATTY 2 cut(s) 142, 494
XbaI TCTAGA 2 cut(s) 208, 319
XceI RCATGY 1 cut(s) 161
XcmI CCANNNNNNNNNTGG 2 cut(s) 536, 754
XhoI CTCGAG 1 cut(s) 701
XspI CTAG 5 cut(s) 77, 209, 296, 320, 408
ZraI GACGTC 1 cut(s) 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.