RLG00000013919

NADP-dependent D-sorbitol-6-phosphate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003235
Physical Location & Seq
Reverse (-)
44260722 .. 44268589
7868 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013919

Sequence Viewer

Length: 930 bp
ATGTCGATAACACTCAACAGCGGGTTCAAAATGCCAGTAATTGGTCTTGGTGTTTGGCGCATGGAAGGAAAAGAAATCAGAGACCTCATCATCAATTCCATCAACATCGGCTATCGCCATTTCGACTGCGCTGCTGATTACAAGAATGAATCAGAAGTTGGAGAGGCACTAGCAGAAGCATTGTCCACTGGGCTTGTTAAGCGAGAGGATCTCTTCATCACCACCAAGCTTTGGAATTCAGATCACGGACATGTTGTGGAGGCCTGTAAAGACAGTCTAAAGAAGCTTCGGTTGGATTATCTAGATCTGTACCTTGTTCACTTTCCTGTTGCCACGAAGCACACCGGAGTTGGTGCAACTGACAGTGCTTTGGATGAGGATGGGGTGCTAGAGATAGACACAACCATTTCTCTAGAAACTACTTGGCATGCTATGGAAGATCTGGTTTCTCTGGGTTTAGTTCGTAGTATTGGGATCAGCAACTATGACATCTTTCTCACTAGAGATTGCCTAGCCTATTCCAAAGTGAAGCCTGCTGTGAATCAGATTGAAACTCACCCATACTTCCAGCGTGATTCTCTTGTCAAATTCTGTCTGAAACACGGCATCTCTGTCACAGCCCACACTCCACTTGGAGGTGCTGCAGCCAACACTGAATGGTTTGGTTCAGTTTCAGCTCCGGATGATCCAGTTCTCAAGAATCTTGCTGAGAAATACAAAAAGACAGTAGCCCAGGTTGTTCTTCGATGGGGCATTCAACGCAACACAGTTGTAATCCCGAAGACGTCAAAACTTGAGAGATTGAAAGAGAAATTTCAAGTCTTCGACTTTGAGATTTCCAAAGAGGACATGGACCTGATTAAAAAGGTGGACAGGAAACTCCGGACTAATCAACCTGCCAAGTTTTGGGGCGTTGATCTATTTGCTTAG

Protein Analysis

310

Amino Acids

34.73

Weight (kDa)

6.32

Isoelectric Point (pI)

27.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 14 - 291 6.1e-52 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 788
Acc36I ACCTGC 1 cut(s) 904
AccB7I CCANNNNNTGG 3 cut(s) 41, 231, 906
AccIII TCCGGA 2 cut(s) 679, 882
AciI CCGC 1 cut(s) 21
AclWI GGATC 3 cut(s) 216, 482, 680
AcsI RAATTY 3 cut(s) 235, 587, 812
AcyI GRCGYC 1 cut(s) 785
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 4 cut(s) 41, 231, 635, 906
AflIII ACRYGT 1 cut(s) 250
AgsI TTSAA 5 cut(s) 28, 551, 758, 805, 818
AjnI CCWGG 1 cut(s) 732
AjuI GAANNNNNNNTTGG 4 cut(s) 141, 173, 275, 307
AluBI AGCT 3 cut(s) 229, 286, 677
AluI AGCT 3 cut(s) 229, 286, 677
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 3 cut(s) 216, 482, 680
Aor13HI TCCGGA 2 cut(s) 679, 882
AoxI GGCC 1 cut(s) 261
ApeKI GCWGC 3 cut(s) 131, 641, 644
ApoI RAATTY 3 cut(s) 235, 587, 812
AspLEI GCGC 2 cut(s) 60, 131
AspS9I GGNCC 1 cut(s) 853
AsuHPI GGTGA 2 cut(s) 211, 548
AvaII GGWCC 1 cut(s) 853
BbsI GAAGAC 2 cut(s) 788, 814
BbvI GCAGC 3 cut(s) 118, 628, 656
BccI CCATC 3 cut(s) 107, 374, 741
BceAI ACGGC 1 cut(s) 619
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 1 cut(s) 734
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 6 cut(s) 170, 302, 389, 413, 501, 512
BfmI CTRYAG 1 cut(s) 642
BfuAI ACCTGC 1 cut(s) 904
BglII AGATCT 2 cut(s) 304, 439
BisI GCNGC 3 cut(s) 132, 642, 645
BlsI GCNGC 3 cut(s) 133, 643, 646
Bme1390I CCNGG 1 cut(s) 734
Bme18I GGWCC 1 cut(s) 853
BmgT120I GGNCC 1 cut(s) 853
BmrFI CCNGG 1 cut(s) 734
BmrI ACTGGG 1 cut(s) 198
BmsI GCATC 1 cut(s) 615
BmuI ACTGGG 1 cut(s) 198
BpiI GAAGAC 2 cut(s) 788, 814
BplI GAGNNNNNCTC 2 cut(s) 195, 227
BpuEI CTTGAG 2 cut(s) 680, 815
BsaHI GRCGYC 1 cut(s) 785
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 732
BsaWI WCCGGW 3 cut(s) 344, 679, 882
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 231, 635, 906
Bse1I ACTGG 3 cut(s) 35, 193, 689
BseAI TCCGGA 2 cut(s) 679, 882
BseBI CCWGG 1 cut(s) 734
BseDI CCNNGG 1 cut(s) 732
BseGI GGATG 3 cut(s) 379, 385, 688
BseLI CCNNNNNNNGG 4 cut(s) 41, 231, 635, 906
BseMII CTCAG 1 cut(s) 699
BseNI ACTGG 3 cut(s) 35, 193, 689
BseXI GCAGC 3 cut(s) 118, 628, 656
BshFI GGCC 1 cut(s) 263
BsiSI CCGG 3 cut(s) 345, 680, 883
BslI CCNNNNNNNGG 4 cut(s) 41, 231, 635, 906
BsmAI GTCTC 1 cut(s) 75
BsmI GAATGC 1 cut(s) 753
BsnI GGCC 1 cut(s) 263
Bso31I GGTCTC 1 cut(s) 75
Bsp13I TCCGGA 2 cut(s) 679, 882
Bsp143I GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
BspACI CCGC 1 cut(s) 21
BspANI GGCC 1 cut(s) 263
BspCNI CTCAG 1 cut(s) 700
BspEI TCCGGA 2 cut(s) 679, 882
BspMAI CTGCAG 1 cut(s) 646
BspMI ACCTGC 1 cut(s) 904
BspPI GGATC 3 cut(s) 216, 482, 680
BspTNI GGTCTC 1 cut(s) 75
BsrI ACTGG 3 cut(s) 35, 193, 689
BssECI CCNNGG 1 cut(s) 732
BssMI GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
BssNI GRCGYC 1 cut(s) 785
Bst2UI CCWGG 1 cut(s) 734
Bst4CI ACNGT 4 cut(s) 275, 365, 727, 769
Bst6I CTCTTC 1 cut(s) 218
BstACI GRCGYC 1 cut(s) 785
BstC8I GCNNGC 2 cut(s) 429, 534
BstDEI CTNAG 2 cut(s) 708, 927
BstF5I GGATG 3 cut(s) 379, 385, 688
BstHHI GCGC 2 cut(s) 60, 131
BstKTI GATC 7 cut(s) 211, 244, 307, 442, 477, 688, 919
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
BstMWI GCNNNNNNNGC 2 cut(s) 199, 759
BstNI CCWGG 1 cut(s) 734
BstNSI RCATGY 2 cut(s) 254, 431
BstSCI CCNGG 1 cut(s) 732
BstSFI CTRYAG 1 cut(s) 642
BstV1I GCAGC 3 cut(s) 118, 628, 656
BstV2I GAAGAC 2 cut(s) 788, 814
BstX2I RGATCY 3 cut(s) 208, 304, 439
BstYI RGATCY 3 cut(s) 208, 304, 439
BsuRI GGCC 1 cut(s) 263
BtsCI GGATG 3 cut(s) 379, 385, 688
BtsIMutI CAGTG 3 cut(s) 186, 370, 651
BveI ACCTGC 1 cut(s) 904
Cac8I GCNNGC 2 cut(s) 429, 534
CfoI GCGC 2 cut(s) 60, 131
Cfr13I GGNCC 1 cut(s) 853
Csp6I GTAC 1 cut(s) 310
CspCI CAANNNNNGTGG 2 cut(s) 175, 210
CviAII CATG 4 cut(s) 61, 251, 428, 850
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 2 cut(s) 708, 927
DpnI GATC 7 cut(s) 210, 243, 306, 441, 476, 687, 918
DpnII GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Eco147I AGGCCT 1 cut(s) 263
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 853
EcoRI GAATTC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 732
FaeI CATG 4 cut(s) 64, 254, 431, 853
FaiI YATR 7 cut(s) 62, 252, 429, 434, 486, 562, 851
FatI CATG 4 cut(s) 60, 250, 427, 849
FauI CCCGC 1 cut(s) 14
Fnu4HI GCNGC 3 cut(s) 132, 642, 645
FokI GGATG 3 cut(s) 386, 392, 695
Fsp4HI GCNGC 3 cut(s) 132, 642, 645
FspBI CTAG 6 cut(s) 170, 302, 389, 413, 501, 512
GlaI GCGC 2 cut(s) 59, 130
GluI GCNGC 3 cut(s) 132, 642, 645
HaeIII GGCC 1 cut(s) 263
HapII CCGG 3 cut(s) 345, 680, 883
HhaI GCGC 2 cut(s) 60, 131
Hin1I GRCGYC 1 cut(s) 785
Hin1II CATG 4 cut(s) 64, 254, 431, 853
Hin6I GCGC 2 cut(s) 58, 129
HinP1I GCGC 2 cut(s) 58, 129
HindIII AAGCTT 2 cut(s) 227, 284
HinfI GANTC 4 cut(s) 149, 541, 575, 700
HpaII CCGG 3 cut(s) 345, 680, 883
HphI GGTGA 2 cut(s) 211, 548
Hpy166II GTNNAC 3 cut(s) 186, 319, 871
Hpy188I TCNGA 5 cut(s) 80, 154, 241, 546, 597
Hpy188III TCNNGA 6 cut(s) 302, 413, 680, 697, 778, 883
Hpy8I GTNNAC 3 cut(s) 186, 319, 871
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 4 cut(s) 275, 365, 727, 769
HpyCH4IV ACGT 1 cut(s) 785
HpyCH4V TGCA 2 cut(s) 356, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 199, 759
HpyF3I CTNAG 2 cut(s) 708, 927
HpySE526I ACGT 1 cut(s) 785
Hsp92I GRCGYC 1 cut(s) 785
Hsp92II CATG 4 cut(s) 64, 254, 431, 853
HspAI GCGC 2 cut(s) 58, 129
Kpn2I TCCGGA 2 cut(s) 679, 882
Kzo9I GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
LmnI GCTCC 1 cut(s) 682
Lsp1109I GCAGC 3 cut(s) 118, 628, 656
LweI GCATC 1 cut(s) 615
MaeI CTAG 6 cut(s) 170, 302, 389, 413, 501, 512
MaeII ACGT 1 cut(s) 785
MaeIII GTNAC 1 cut(s) 613
MalI GATC 7 cut(s) 210, 243, 306, 441, 476, 687, 918
MboI GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
MboII GAAGA 5 cut(s) 205, 449, 734, 793, 814
MflI RGATCY 3 cut(s) 208, 304, 439
MluCI AATT 5 cut(s) 39, 94, 235, 587, 812
MmeI TCCRAC 2 cut(s) 139, 273
MnlI CCTC 7 cut(s) 95, 157, 199, 253, 370, 629, 838
MroI TCCGGA 2 cut(s) 679, 882
MseI TTAA 2 cut(s) 198, 861
MslI CAYNNNNRTG 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 21
MspI CCGG 3 cut(s) 345, 680, 883
MspR9I CCNGG 1 cut(s) 734
Mva1269I GAATGC 1 cut(s) 753
MvaI CCWGG 1 cut(s) 734
MwoI GCNNNNNNNGC 2 cut(s) 199, 759
NdeII GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
NlaIII CATG 4 cut(s) 64, 254, 431, 853
NmuCI GTSAC 1 cut(s) 613
NspI RCATGY 2 cut(s) 254, 431
PaeI GCATGC 1 cut(s) 431
PceI AGGCCT 1 cut(s) 263
PciI ACATGT 1 cut(s) 250
PctI GAATGC 1 cut(s) 753
PfeI GAWTC 4 cut(s) 149, 541, 575, 700
PflMI CCANNNNNTGG 3 cut(s) 41, 231, 906
PkrI GCNGC 3 cut(s) 133, 643, 646
PscI ACATGT 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 732
PspGI CCWGG 1 cut(s) 732
PspPI GGNCC 1 cut(s) 853
PstI CTGCAG 1 cut(s) 646
PsuI RGATCY 3 cut(s) 208, 304, 439
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 2 cut(s) 198, 861
SatI GCNGC 3 cut(s) 132, 642, 645
Sau3AI GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
Sau96I GGNCC 1 cut(s) 853
ScrFI CCNGG 1 cut(s) 734
SfaNI GCATC 1 cut(s) 615
SfcI CTRYAG 1 cut(s) 642
SinI GGWCC 1 cut(s) 853
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 695, 794
SmoI CTYRAG 2 cut(s) 695, 794
SphI GCATGC 1 cut(s) 431
Sse9I AATT 5 cut(s) 39, 94, 235, 587, 812
SseBI AGGCCT 1 cut(s) 263
SsiI CCGC 1 cut(s) 21
SspMI CTAG 6 cut(s) 170, 302, 389, 413, 501, 512
StuI AGGCCT 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 732
TaaI ACNGT 4 cut(s) 275, 365, 727, 769
TaiI ACGT 1 cut(s) 788
TaqI TCGA 4 cut(s) 5, 123, 745, 825
TasI AATT 5 cut(s) 39, 94, 235, 587, 812
TfiI GAWTC 4 cut(s) 149, 541, 575, 700
Tru1I TTAA 2 cut(s) 198, 861
Tru9I TTAA 2 cut(s) 198, 861
TscAI CASTG 3 cut(s) 193, 370, 658
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 3 cut(s) 131, 641, 644
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 2 cut(s) 162, 205
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 3 cut(s) 193, 370, 658
Van91I CCANNNNNTGG 3 cut(s) 41, 231, 906
VpaK11BI GGWCC 1 cut(s) 853
XapI RAATTY 3 cut(s) 235, 587, 812
XbaI TCTAGA 2 cut(s) 301, 412
XceI RCATGY 2 cut(s) 254, 431
XcmI CCANNNNNNNNNTGG 2 cut(s) 629, 847
XspI CTAG 6 cut(s) 170, 302, 389, 413, 501, 512
ZraI GACGTC 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.