FvH4_2g10630

NADP-dependent D-sorbitol-6-phosphate dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003235
Physical Location & Seq
Forward (+)
9393818 .. 9398054
4237 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g10630.t1

Sequence Viewer

Length: 930 bp
ATGTCTGTAACACTCAACAGCGGGTTCAAAATGCCGGTTCTCGGTCTTGGCGTTTGGCGCATGGAAGGAAAAGAAATCAGAGACCTCATCATTAATTCCATCAACATTGGTTATCGCCATTTCGACTGCGCTGCTGATTACAAGAATGAGTCAGAAGTTGGAGACGCACTGGCAGAAGCAATTTCCACTGGGCATGTTAAGCGAGAGGATCTCTTCATCACCACCAAGCTTTGGAATTCAGATCATGGACATGTTGTGGAGGCGTGTAAAGACAGTCTAAAGAAGCTTCGGTTGGATTATCTAGATCTGTATCTTGTTCACTTTCCTGTTGCTACGAAGCACACTGGAGTTGGTGCAACTGGCAGTGCTTTGGATGAGGAAGGGGTGCTAGAGATAGACACAACCATTTCTCTAGAAACTACTTGGCATGCTATGGAAGATCTGGTTTCTCTGGGTTTAGTTCGTAGTATTGGGATCAGCAACTACGACATCTTTCTCACTAGAGATTGCTTAGCCTATTCCAAAGTGAAGCCTGCTGTGAATCAGATTGAAACTCACCCATACTTCCAGCGTGATTCTCTTGTCAAATTCTGTCTGAAGCATGGCATCTCTGTCACAGCCCACACTCCACTGGGAGGTGCTGCAGCCAACACTGAATGGTTTGGCTCAGTTTCACCTCTGGATGATCCAGTTCTCAAGAATCTTGCTGTGAAATACAAAAAGACAGTAGCCCAGGTTGTTCTTCAATGGGGCATTCAACGCAACACGGTTGTAATCCCGAAGACATCAAAACTCGAGAGATTGAAAGAGAATTTTCAAGTCTTTGACTTTGAGATTTCAAAAGAGGACATGGACCTGATTAAAAAGGTGGACAAGAATCTCCGGACCAATCAACCTGCGAAGTTTTGGGGCGTTGATCTATATGCTTAG

Protein Analysis

310

Amino Acids

34.63

Weight (kDa)

6.21

Isoelectric Point (pI)

26.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 14 - 291 1e-51 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 904
AccB7I CCANNNNNTGG 1 cut(s) 231
AccIII TCCGGA 1 cut(s) 882
AciI CCGC 1 cut(s) 21
AclWI GGATC 3 cut(s) 216, 482, 680
AcsI RAATTY 3 cut(s) 235, 587, 811
AcuI CTGAAG 1 cut(s) 617
AfiI CCNNNNNNNGG 3 cut(s) 41, 231, 635
AflIII ACRYGT 1 cut(s) 250
AgsI TTSAA 7 cut(s) 28, 551, 746, 758, 805, 818, 840
AjnI CCWGG 1 cut(s) 732
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AluBI AGCT 2 cut(s) 229, 286
AluI AGCT 2 cut(s) 229, 286
Alw26I GTCTC 2 cut(s) 75, 156
AlwI GGATC 3 cut(s) 216, 482, 680
Ama87I CYCGRG 1 cut(s) 794
Aor13HI TCCGGA 1 cut(s) 882
ApeKI GCWGC 3 cut(s) 131, 641, 644
ApoI RAATTY 3 cut(s) 235, 587, 811
AseI ATTAAT 1 cut(s) 93
AspLEI GCGC 2 cut(s) 60, 131
AspS9I GGNCC 2 cut(s) 853, 885
AsuHPI GGTGA 3 cut(s) 211, 548, 666
AvaI CYCGRG 1 cut(s) 794
AvaII GGWCC 2 cut(s) 853, 885
BbsI GAAGAC 1 cut(s) 788
BbvI GCAGC 3 cut(s) 118, 628, 656
BccI CCATC 1 cut(s) 107
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 1 cut(s) 734
BcoDI GTCTC 2 cut(s) 75, 156
BfaI CTAG 4 cut(s) 302, 389, 413, 501
BfmI CTRYAG 1 cut(s) 642
BfuAI ACCTGC 1 cut(s) 904
BglII AGATCT 2 cut(s) 304, 439
BisI GCNGC 3 cut(s) 132, 642, 645
BlpI GCTNAGC 1 cut(s) 511
BlsI GCNGC 3 cut(s) 133, 643, 646
Bme1390I CCNGG 1 cut(s) 734
Bme18I GGWCC 2 cut(s) 853, 885
BmeT110I CYCGRG 1 cut(s) 794
BmgT120I GGNCC 2 cut(s) 853, 885
BmrFI CCNGG 1 cut(s) 734
BmrI ACTGGG 2 cut(s) 198, 641
BmsI GCATC 1 cut(s) 615
BmuI ACTGGG 2 cut(s) 198, 641
BpiI GAAGAC 1 cut(s) 788
BplI GAGNNNNNCTC 2 cut(s) 195, 227
BpmI CTGGAG 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 511
BpuEI CTTGAG 1 cut(s) 680
BsaBI GATNNNNATC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 732
BsaWI WCCGGW 1 cut(s) 882
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 231, 635
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 6 cut(s) 174, 193, 349, 364, 636, 689
Bse8I GATNNNNATC 1 cut(s) 309
BseAI TCCGGA 1 cut(s) 882
BseBI CCWGG 1 cut(s) 734
BseDI CCNNGG 1 cut(s) 732
BseGI GGATG 2 cut(s) 379, 688
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 3 cut(s) 41, 231, 635
BseMII CTCAG 1 cut(s) 681
BseNI ACTGG 6 cut(s) 174, 193, 349, 364, 636, 689
BseXI GCAGC 3 cut(s) 118, 628, 656
BsiHKCI CYCGRG 1 cut(s) 794
BsiSI CCGG 2 cut(s) 35, 883
BslI CCNNNNNNNGG 3 cut(s) 41, 231, 635
BsmAI GTCTC 2 cut(s) 75, 156
BsmBI CGTCTC 1 cut(s) 156
BsmI GAATGC 1 cut(s) 753
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 1 cut(s) 794
Bsp13I TCCGGA 1 cut(s) 882
Bsp143I GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
Bsp1720I GCTNAGC 1 cut(s) 511
BspACI CCGC 1 cut(s) 21
BspCNI CTCAG 1 cut(s) 680
BspEI TCCGGA 1 cut(s) 882
BspMAI CTGCAG 1 cut(s) 646
BspMI ACCTGC 1 cut(s) 904
BspPI GGATC 3 cut(s) 216, 482, 680
BspTNI GGTCTC 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 6 cut(s) 174, 193, 349, 364, 636, 689
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 732
BssMI GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
Bst2UI CCWGG 1 cut(s) 734
Bst4CI ACNGT 3 cut(s) 275, 727, 769
Bst6I CTCTTC 1 cut(s) 218
BstC8I GCNNGC 2 cut(s) 429, 534
BstDEI CTNAG 3 cut(s) 511, 667, 927
BstF5I GGATG 2 cut(s) 379, 688
BstHHI GCGC 2 cut(s) 60, 131
BstKTI GATC 7 cut(s) 211, 244, 307, 442, 477, 688, 919
BstMAI GTCTC 2 cut(s) 75, 156
BstMBI GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
BstMWI GCNNNNNNNGC 3 cut(s) 57, 199, 759
BstNI CCWGG 1 cut(s) 734
BstNSI RCATGY 3 cut(s) 197, 254, 431
BstSCI CCNGG 1 cut(s) 732
BstSFI CTRYAG 1 cut(s) 642
BstV1I GCAGC 3 cut(s) 118, 628, 656
BstV2I GAAGAC 1 cut(s) 788
BstX2I RGATCY 3 cut(s) 208, 304, 439
BstYI RGATCY 3 cut(s) 208, 304, 439
BtsCI GGATG 2 cut(s) 379, 688
BtsI GCAGTG 1 cut(s) 370
BtsIMutI CAGTG 6 cut(s) 167, 186, 342, 370, 629, 651
BveI ACCTGC 1 cut(s) 904
Cac8I GCNNGC 2 cut(s) 429, 534
CfoI GCGC 2 cut(s) 60, 131
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 853, 885
CseI GACGC 1 cut(s) 173
CviAII CATG 7 cut(s) 61, 194, 245, 251, 428, 602, 850
CviJI RGCY 8 cut(s) 229, 286, 515, 532, 620, 647, 666, 731
CviKI_1 RGCY 8 cut(s) 229, 286, 515, 532, 620, 647, 666, 731
DdeI CTNAG 3 cut(s) 511, 667, 927
DpnI GATC 7 cut(s) 210, 243, 306, 441, 476, 687, 918
DpnII GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 853, 885
Eco57I CTGAAG 1 cut(s) 617
Eco88I CYCGRG 1 cut(s) 794
EcoRI GAATTC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 732
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 7 cut(s) 64, 197, 248, 254, 431, 605, 853
FatI CATG 7 cut(s) 60, 193, 244, 250, 427, 601, 849
FauI CCCGC 1 cut(s) 14
Fnu4HI GCNGC 3 cut(s) 132, 642, 645
FokI GGATG 2 cut(s) 386, 695
Fsp4HI GCNGC 3 cut(s) 132, 642, 645
FspBI CTAG 4 cut(s) 302, 389, 413, 501
GlaI GCGC 2 cut(s) 59, 130
GluI GCNGC 3 cut(s) 132, 642, 645
GsuI CTGGAG 1 cut(s) 366
HapII CCGG 2 cut(s) 35, 883
HgaI GACGC 1 cut(s) 173
HhaI GCGC 2 cut(s) 60, 131
Hin1II CATG 7 cut(s) 64, 197, 248, 254, 431, 605, 853
Hin6I GCGC 2 cut(s) 58, 129
HinP1I GCGC 2 cut(s) 58, 129
HindIII AAGCTT 2 cut(s) 227, 284
HinfI GANTC 5 cut(s) 149, 541, 575, 700, 877
HpaII CCGG 2 cut(s) 35, 883
HphI GGTGA 3 cut(s) 211, 548, 666
Hpy166II GTNNAC 2 cut(s) 319, 871
Hpy188I TCNGA 5 cut(s) 80, 154, 241, 546, 597
Hpy188III TCNNGA 7 cut(s) 302, 413, 680, 697, 778, 796, 883
Hpy8I GTNNAC 2 cut(s) 319, 871
HpyAV CCTTC 2 cut(s) 59, 374
HpyCH4III ACNGT 3 cut(s) 275, 727, 769
HpyCH4V TGCA 2 cut(s) 356, 644
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 199, 759
HpyF3I CTNAG 3 cut(s) 511, 667, 927
Hsp92II CATG 7 cut(s) 64, 197, 248, 254, 431, 605, 853
HspAI GCGC 2 cut(s) 58, 129
Kpn2I TCCGGA 1 cut(s) 882
Kzo9I GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
Lsp1109I GCAGC 3 cut(s) 118, 628, 656
LweI GCATC 1 cut(s) 615
MaeI CTAG 4 cut(s) 302, 389, 413, 501
MaeIII GTNAC 2 cut(s) 7, 613
MalI GATC 7 cut(s) 210, 243, 306, 441, 476, 687, 918
MboI GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
MboII GAAGA 4 cut(s) 205, 449, 734, 793
MflI RGATCY 3 cut(s) 208, 304, 439
MluCI AATT 5 cut(s) 94, 180, 235, 587, 811
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 2 cut(s) 139, 273
MnlI CCTC 7 cut(s) 95, 199, 253, 370, 629, 687, 838
MroI TCCGGA 1 cut(s) 882
MseI TTAA 3 cut(s) 93, 198, 861
MslI CAYNNNNRTG 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 21
MspI CCGG 2 cut(s) 35, 883
MspR9I CCNGG 1 cut(s) 734
Mva1269I GAATGC 1 cut(s) 753
MvaI CCWGG 1 cut(s) 734
MwoI GCNNNNNNNGC 3 cut(s) 57, 199, 759
NdeII GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
NlaIII CATG 7 cut(s) 64, 197, 248, 254, 431, 605, 853
NmuCI GTSAC 1 cut(s) 613
NspI RCATGY 3 cut(s) 197, 254, 431
PaeI GCATGC 1 cut(s) 431
PaeR7I CTCGAG 1 cut(s) 794
PciI ACATGT 1 cut(s) 250
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 753
PfeI GAWTC 4 cut(s) 541, 575, 700, 877
PflMI CCANNNNNTGG 1 cut(s) 231
PkrI GCNGC 3 cut(s) 133, 643, 646
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PscI ACATGT 1 cut(s) 250
PshBI ATTAAT 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 732
PspGI CCWGG 1 cut(s) 732
PspPI GGNCC 2 cut(s) 853, 885
PstI CTGCAG 1 cut(s) 646
PsuI RGATCY 3 cut(s) 208, 304, 439
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 3 cut(s) 93, 198, 861
SatI GCNGC 3 cut(s) 132, 642, 645
Sau3AI GATC 7 cut(s) 208, 241, 304, 439, 474, 685, 916
Sau96I GGNCC 2 cut(s) 853, 885
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 734
SetI ASST 9 cut(s) 87, 231, 288, 640, 679, 738, 858, 870, 898
SfaNI GCATC 1 cut(s) 615
SfcI CTRYAG 1 cut(s) 642
Sfr274I CTCGAG 1 cut(s) 794
SinI GGWCC 2 cut(s) 853, 885
SlaI CTCGAG 1 cut(s) 794
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 695, 794
SmoI CTYRAG 2 cut(s) 695, 794
SphI GCATGC 1 cut(s) 431
Sse9I AATT 5 cut(s) 94, 180, 235, 587, 811
SsiI CCGC 1 cut(s) 21
SspMI CTAG 4 cut(s) 302, 389, 413, 501
StyD4I CCNGG 1 cut(s) 732
TaaI ACNGT 3 cut(s) 275, 727, 769
TaqI TCGA 2 cut(s) 123, 795
TaqII GACCGA 1 cut(s) 32
TasI AATT 5 cut(s) 94, 180, 235, 587, 811
TfiI GAWTC 4 cut(s) 541, 575, 700, 877
Tru1I TTAA 3 cut(s) 93, 198, 861
Tru9I TTAA 3 cut(s) 93, 198, 861
TscAI CASTG 6 cut(s) 174, 193, 349, 370, 636, 658
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 3 cut(s) 131, 641, 644
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 1 cut(s) 205
TspRI CASTG 6 cut(s) 174, 193, 349, 370, 636, 658
Van91I CCANNNNNTGG 1 cut(s) 231
VpaK11BI GGWCC 2 cut(s) 853, 885
VspI ATTAAT 1 cut(s) 93
XapI RAATTY 3 cut(s) 235, 587, 811
XbaI TCTAGA 2 cut(s) 301, 412
XceI RCATGY 3 cut(s) 197, 254, 431
XhoI CTCGAG 1 cut(s) 794
XspI CTAG 4 cut(s) 302, 389, 413, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.